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New Enzymatic Approach to Distinguish Fucosylation Isomers of N-Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry

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    New Enzymatic Approach to Distinguish Fucosylation Isomers of N-Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry
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    • Connor A. West
      Connor A. West
      Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 173 Ashley Avenue, Basic Science Building 358, Charleston South Carolina 29425, United States
      More by Connor A. West
    • Hongyan Liang
      Hongyan Liang
      Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 173 Ashley Avenue, Basic Science Building 358, Charleston South Carolina 29425, United States
      More by Hongyan Liang
    • Richard R. Drake
      Richard R. Drake
      Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 173 Ashley Avenue, Basic Science Building 358, Charleston South Carolina 29425, United States
      More by Richard R. Drake
    • Anand S. Mehta*
      Anand S. Mehta
      Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 173 Ashley Avenue, Basic Science Building 358, Charleston South Carolina 29425, United States
      *Email: [email protected]. Phone: 843-792-9946.
      More by Anand S. Mehta
      Orcidhttp://orcid.org/0000-0002-9846-9389
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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2020, 19, 8, 2989–2996
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    https://doi.org/10.1021/acs.jproteome.0c00024
    Published May 22, 2020
    Copyright © 2020 American Chemical Society
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    Abstract

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    Specific alterations in N-linked glycans, such as core fucosylation, are associated with many cancers and other disease states. Because of the many possible anomeric linkages associated with fucosylated N-glycans, determination of specific anomeric linkages and the site of fucosylation (i.e., core vs outer arm) can be difficult to elucidate. A new MALDI mass spectrometry imaging workflow in formalin-fixed clinical tissues is described using recombinant endoglycosidase F3 (Endo F3), an enzyme with a specific preference for cleaving core-fucosylated N-glycans attached to glycoproteins. In contrast to the broader substrate enzyme peptide-N-glycosidase F (PNGaseF), Endo F3 cleaves between the two core N-acetylglucosamine residues at the protein attachment site. On tissues, this results in a mass shift of 349.137 a.m.u. for core-fucosylated N-glycans when compared to N-glycans released with standard PNGaseF. Endo F3 can be used singly and in combination with PNGaseF digestion of the same tissue sections. Initial results in liver and prostate tissues indicate core-fucosylated glycans associated to specific tissue regions while still demonstrating a diverse mix of core- and outer arm-fucosylated glycans throughout all regions of tissue. By determining these specific linkages while preserving localization, more targeted diagnostic biomarkers for disease states are possible without the need for microdissection or solubilization of the tissue.

    Copyright © 2020 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jproteome.0c00024.

    • Hematoxylin and eosin stain of prostate cancer tissue, multiple tissue types treated with Endo F3, mixture of PNGase F and Endo F3, survival probability of noncore-fucosylated N-linked glycans, full SDS-PAGE gel image, master list of N-linked glycans with PNGase F, and master list of N-linked glycans with Endo F3 (PDF)

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    Cited By

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    This article is cited by 37 publications.

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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2020, 19, 8, 2989–2996
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jproteome.0c00024
    Published May 22, 2020
    Copyright © 2020 American Chemical Society
    Request reuse permissions

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