ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Figure 1Loading Img

Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach

  • Huanchao Zeng
    Huanchao Zeng
    Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
  • Yiru Qin
    Yiru Qin
    Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
    More by Yiru Qin
  • Erxia Du
    Erxia Du
    Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
    More by Erxia Du
  • Qiulan Wei
    Qiulan Wei
    Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    More by Qiulan Wei
  • Ying Li
    Ying Li
    Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    More by Ying Li
  • Danyan Huang
    Danyan Huang
    Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    More by Danyan Huang
  • Guirong Wang
    Guirong Wang
    Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China
    More by Guirong Wang
  • Jan A. Veenstra
    Jan A. Veenstra
    INCIA, UMR 5287 CNRS, Université de Bordeaux, Pessac F33615, France
  • Sheng Li
    Sheng Li
    Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
    More by Sheng Li
  • , and 
  • Na Li*
    Na Li
    Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
    *Email: [email protected]
    More by Na Li
Cite this: J. Proteome Res. 2021, 20, 2, 1217–1228
Publication Date (Web):November 9, 2020
https://doi.org/10.1021/acs.jproteome.0c00596
Copyright © 2020 American Chemical Society

    Article Views

    1026

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (1)»

    Abstract

    Abstract Image

    As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, is of great interest in both basic and applied research. Previous studies on P. americana neuropeptide identification have been based on biochemical isolation and molecular cloning. In the present study, an integrated approach of genomics- and peptidomics-based discovery was performed for neuropeptide identification in this insect species. First, 67 conserved neuropeptide or neurohormone precursor genes were predicted via an in silico analysis of the P. americana genome and transcriptome. Using a large-scale peptidomic analysis of peptide extracts from four different tissues (the central nervous system, corpora cardiac and corpora allata complex, midgut, and male accessory gland), 35 conserved (predicted) neuropeptides and a potential (novel) neuropeptide were then identified. Subsequent experiments revealed the tissue distribution, sex difference, and developmental patterns of two conserved neuropeptides (allatostatin B and short neuropeptide F) and a novel neuropeptide (PaOGS36577). Our study shows a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, providing a solid basis for future functional studies of neuropeptides in the American cockroach (data are available via ProteomeXchange with identifier PXD021660).

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jproteome.0c00596.

    • Primers used for qRT-PCR (Table S1); predicted neuropeptide transcript not validated by MS (Table S1); amino acid sequence for neuropeptide precursors detected by MS (Figure S1); amino acid sequence for predicted neuropeptide precursors not validated by MS (Figure S2); and alignment analysis of PaOGS36577 and homologues (Figure S3) (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 20 publications.

    1. Paweł Marciniak, Joanna Pacholska-Bogalska, Lapo Ragionieri. Neuropeptidomes of Tenebrio molitor L. and Zophobas atratus Fab. (Coleoptera, Polyphaga: Tenebrionidae). Journal of Proteome Research 2022, 21 (10) , 2247-2260. https://doi.org/10.1021/acs.jproteome.1c00694
    2. Shiming Zhu, Xiaoyi Chen, Sishi Xia, Qin Li, Ziqi Ye, Shaoting Zhao, Kexin Liu, Fangfang Liu. Hexamerin and allergen are required for female reproduction in the American cockroach, Periplaneta americana. Insect Science 2024, 31 (1) , 186-200. https://doi.org/10.1111/1744-7917.13218
    3. Chunyang Huang, Xiangli Dong, Xiang Yang, Jingmiao Zou, Mingwei Yang, Xinyi Wang, Wenhong Li, Yueping He. Identification of neuropeptides and their G protein‐coupled receptors in the predatory stink bug, Arma custos (Hemiptera: Pentatomidae). Archives of Insect Biochemistry and Physiology 2024, 115 (2) https://doi.org/10.1002/arch.22094
    4. Roland Hellinger, Arnar Sigurdsson, Wenxin Wu, Elena V. Romanova, Lingjun Li, Jonathan V. Sweedler, Roderich D. Süssmuth, Christian W. Gruber. Peptidomics. Nature Reviews Methods Primers 2023, 3 (1) https://doi.org/10.1038/s43586-023-00205-2
    5. Shixiong Jiang, Heather G. Marco, Nina Scheich, Shulin He, Zongqing Wang, Gerd Gäde, Dino P. McMahon. Comparative analysis of adipokinetic hormones and their receptors in Blattodea reveals novel patterns of gene evolution. Insect Molecular Biology 2023, 32 (6) , 615-633. https://doi.org/10.1111/imb.12861
    6. Heather G. Marco, Simone König, Gerd Gäde. Predicted novel hypertrehalosaemic peptides of cockroaches are verified by mass spectrometry. Amino Acids 2023, 55 (11) , 1641-1654. https://doi.org/10.1007/s00726-023-03337-7
    7. Li‐Lin Luo, Yang Lin, Jun‐Hong Linghu, Wei Gong, Yuan‐Hong Luo, Man Liu, Dao‐Chao Jin, Guy Smagghe, Tong‐Xian Liu, Shun‐Hua Gui, Tian‐Ci Yi. Genomics, transcriptomics, and peptidomics of the greater wax moth Galleria mellonella neuropeptides and their expression in response to lead stress. Insect Science 2023, 7 https://doi.org/10.1111/1744-7917.13264
    8. Jan A. Veenstra. Different neuroendocrine cell types in the pars intercerebralis of Periplaneta americana produce their own specific IGF-related peptides. General and Comparative Endocrinology 2023, 335 , 114233. https://doi.org/10.1016/j.ygcen.2023.114233
    9. Mako Sasao, Tomohide Uno, Risa Kitagawa, Asuka Matsui, Fumika Toryu, Akira Mizoguchi, Kengo Kanamaru, Katsuhiko Sakamoto, Yuichi Uno. Localization of SNARE proteins in the brain and corpus allatum of Bombyx mori. Histochemistry and Cell Biology 2023, 159 (2) , 199-208. https://doi.org/10.1007/s00418-022-02153-6
    10. Fangfang Liu, Yingying Cui, Huna Lu, Xiaoyi Chen, Qin Li, Ziqi Ye, Wanyi Chen, Shiming Zhu. Myofilaments promote wing expansion and maintain genitalia morphology in the American cockroach, Periplaneta americana. Insect Molecular Biology 2023, 32 (1) , 46-55. https://doi.org/10.1111/imb.12812
    11. Asuka MATSUI, Makoto TOKUSHIGE, Akira MIZOGUCHI, Kengo KANAMARU, Katsuhiko SAKAMOTO, Yuichi UNO, Tomohide UNO. Locations of Rab, Allatotropin, Prothoracicotropic hormone and Period in the larval brain, corpus allatum and frontal ganglion of Bombyx mori. European Journal of Entomology 2023, 120 , 93-104. https://doi.org/10.14411/eje.2023.012
    12. Jan A. Veenstra. Differential expression of some termite neuropeptides and insulin/IGF-related hormones and their plausible functions in growth, reproduction and caste determination. PeerJ 2023, 11 , e15259. https://doi.org/10.7717/peerj.15259
    13. Vanessa Althaus, Stefanie Jahn, Azar Massah, Monika Stengl, Uwe Homberg. 3D‐atlas of the brain of the cockroach Rhyparobia maderae. Journal of Comparative Neurology 2022, 530 (18) , 3126-3156. https://doi.org/10.1002/cne.25396
    14. Hai-Pan Wu, Xiao-Yun Wang, Jin Hu, Ran-Ran Su, Wen Lu, Xia-Lin Zheng. Identification of neuropeptides and neuropeptide receptor genes in Phauda flammans (Walker). Scientific Reports 2022, 12 (1) https://doi.org/10.1038/s41598-022-13590-7
    15. Jéssica Waldman, Marina Amaral Xavier, Larissa Rezende Vieira, Raquel Logullo, Gloria Regina Cardoso Braz, Lucas Tirloni, José Marcos C. Ribeiro, Jan A. Veenstra, Itabajara da Silva Vaz. Neuropeptides in Rhipicephalus microplus and other hard ticks. Ticks and Tick-borne Diseases 2022, 13 (3) , 101910. https://doi.org/10.1016/j.ttbdis.2022.101910
    16. Lapo Ragionieri, Rik Verdonck, Heleen Verlinden, Elisabeth Marchal, Jozef Vanden Broeck, Reinhard Predel. Schistocerca neuropeptides – An update. Journal of Insect Physiology 2022, 136 , 104326. https://doi.org/10.1016/j.jinsphys.2021.104326
    17. Jan A. Veenstra. Identification of cells expressing Calcitonins A and B, PDF and ACP in Locusta migratoria using cross-reacting antisera and in situ hybridization. Peptides 2021, 146 , 170667. https://doi.org/10.1016/j.peptides.2021.170667
    18. Jens Habenstein, Franziska Schmitt, Sander Liessem, Alice Ly, Dennis Trede, Christian Wegener, Reinhard Predel, Wolfgang Rössler, Susanne Neupert. Transcriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nodus. Journal of Neurochemistry 2021, 158 (2) , 391-412. https://doi.org/10.1111/jnc.15346
    19. Dick R. Nässel, Shun-Fan Wu. Leucokinins: Multifunctional Neuropeptides and Hormones in Insects and Other Invertebrates. International Journal of Molecular Sciences 2021, 22 (4) , 1531. https://doi.org/10.3390/ijms22041531
    20. Dick R. Nässel. Leucokinin and Associated Neuropeptides Regulate Multiple Aspects of Physiology and Behavior in Drosophila. International Journal of Molecular Sciences 2021, 22 (4) , 1940. https://doi.org/10.3390/ijms22041940

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect