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Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach

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    Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach
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    • Huanchao Zeng
      Huanchao Zeng
      Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
      Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
      More by Huanchao Zeng
    • Yiru Qin
      Yiru Qin
      Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
      Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
      More by Yiru Qin
    • Erxia Du
      Erxia Du
      Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
      Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
      More by Erxia Du
    • Qiulan Wei
      Qiulan Wei
      Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
      More by Qiulan Wei
    • Ying Li
      Ying Li
      Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
      More by Ying Li
    • Danyan Huang
      Danyan Huang
      Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
      More by Danyan Huang
    • Guirong Wang
      Guirong Wang
      Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China
      More by Guirong Wang
    • Jan A. Veenstra
      Jan A. Veenstra
      INCIA, UMR 5287 CNRS, Université de Bordeaux, Pessac F33615, France
      More by Jan A. Veenstra
      Orcidhttp://orcid.org/0000-0002-2783-0018
    • Sheng Li
      Sheng Li
      Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
      Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
      More by Sheng Li
    • Na Li*
      Na Li
      Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
      Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China
      *Email: [email protected]
      More by Na Li
      Orcidhttp://orcid.org/0000-0003-4202-6375
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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2021, 20, 2, 1217–1228
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jproteome.0c00596
    Published November 9, 2020
    Copyright © 2020 American Chemical Society
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    Abstract

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    As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, is of great interest in both basic and applied research. Previous studies on P. americana neuropeptide identification have been based on biochemical isolation and molecular cloning. In the present study, an integrated approach of genomics- and peptidomics-based discovery was performed for neuropeptide identification in this insect species. First, 67 conserved neuropeptide or neurohormone precursor genes were predicted via an in silico analysis of the P. americana genome and transcriptome. Using a large-scale peptidomic analysis of peptide extracts from four different tissues (the central nervous system, corpora cardiac and corpora allata complex, midgut, and male accessory gland), 35 conserved (predicted) neuropeptides and a potential (novel) neuropeptide were then identified. Subsequent experiments revealed the tissue distribution, sex difference, and developmental patterns of two conserved neuropeptides (allatostatin B and short neuropeptide F) and a novel neuropeptide (PaOGS36577). Our study shows a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, providing a solid basis for future functional studies of neuropeptides in the American cockroach (data are available via ProteomeXchange with identifier PXD021660).

    Copyright © 2020 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jproteome.0c00596.

    • Primers used for qRT-PCR (Table S1); predicted neuropeptide transcript not validated by MS (Table S1); amino acid sequence for neuropeptide precursors detected by MS (Figure S1); amino acid sequence for predicted neuropeptide precursors not validated by MS (Figure S2); and alignment analysis of PaOGS36577 and homologues (Figure S3) (PDF)

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    This article is cited by 26 publications.

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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2021, 20, 2, 1217–1228
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jproteome.0c00596
    Published November 9, 2020
    Copyright © 2020 American Chemical Society
    Request reuse permissions

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