Proteogenomics Study of Blastobotrys adeninivorans TMCC 70007—A Dominant Yeast in the Fermentation Process of Pu-erh TeaClick to copy article linkArticle link copied!
- Fei TianFei TianKey Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, Research Unit of Proteomics & Research and Development of New Drug, Chinese Academy of Medical Sciences, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, ChinaMore by Fei Tian
- Jiahui ShiJiahui ShiState Key Laboratory of Proteomics, Beijing Proteome Research Center, Research Unit of Proteomics & Research and Development of New Drug, Chinese Academy of Medical Sciences, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, ChinaHebei Province Key Lab of Research and Application on Microbial Diversity, College of Life Sciences, Hebei University, Baoding 071002, ChinaMore by Jiahui Shi
- Yanchang LiYanchang LiState Key Laboratory of Proteomics, Beijing Proteome Research Center, Research Unit of Proteomics & Research and Development of New Drug, Chinese Academy of Medical Sciences, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, ChinaMore by Yanchang Li
- Huiying GaoHuiying GaoState Key Laboratory of Proteomics, Beijing Proteome Research Center, Research Unit of Proteomics & Research and Development of New Drug, Chinese Academy of Medical Sciences, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, ChinaMore by Huiying Gao
- Lei ChangLei ChangState Key Laboratory of Proteomics, Beijing Proteome Research Center, Research Unit of Proteomics & Research and Development of New Drug, Chinese Academy of Medical Sciences, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, ChinaMore by Lei Chang
- Yao ZhangYao ZhangState Key Laboratory of Proteomics, Beijing Proteome Research Center, Research Unit of Proteomics & Research and Development of New Drug, Chinese Academy of Medical Sciences, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, ChinaMore by Yao Zhang
- Linrui GaoLinrui GaoYunnan Pu-erh Tea Fermentation Engineering Research Center, Yunnan TAETEA Microbial Technology Co., Ltd., Kunming 650217, ChinaMore by Linrui Gao
- Ping Xu*Ping Xu*Email: [email protected]State Key Laboratory of Proteomics, Beijing Proteome Research Center, Research Unit of Proteomics & Research and Development of New Drug, Chinese Academy of Medical Sciences, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, ChinaHebei Province Key Lab of Research and Application on Microbial Diversity, College of Life Sciences, Hebei University, Baoding 071002, ChinaMore by Ping Xu
- Shukun Tang*Shukun Tang*Email: [email protected]Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, ChinaYunnan Pu-erh Tea Fermentation Engineering Research Center, Yunnan TAETEA Microbial Technology Co., Ltd., Kunming 650217, ChinaMore by Shukun Tang
Abstract

Blastobotrys adeninivorans plays an essential role in pile-fermenting of Pu-erh tea. Its ability to assimilate various carbon and nitrogen sources makes it available for application in a wide range of industry sectors. The genome of B. adeninivorans TMCC 70007 isolated from pile-fermented Pu-erh tea was sequenced and assembled. Proteomics analysis indicated that 4900 proteins in TMCC 70007 were expressed under various culture conditions. Proteogenomics mapping revealed 48 previously unknown genes and corrected 118 gene models predicted by GeneMark-ES. Ortho-proteogenomics analysis identified 17 previously unidentified genes in B. adeninivorans LS3, the first strain with a sequenced genome among the genus Blastobotrys as well. More importantly, five species specific genes were identified from TMCC 70007, which could serve as a barcode for strain typing and were applicable for fermentation process protection of this industrial species. The datasets generated from tea aqueous extract culture not only increased the proteome coverage and accuracy but also contributed to the identification of proteins related to polyphenols and caffeine, which were considered to change greatly during the microbial fermentation of Pu-erh tea. This study provides a proteome perspective on TMCC 70007, which was considered to be an important strain in the production of Pu-erh tea. The systematic proteogenomics analysis not only made a better annotation on the genome of B. adeninivorans TMCC 70007 as previous proteogenomics study but also provided solution for fermentation process protection on valuable industrial species with species specific genes uniquely identified from proteogenomics study.
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