ACS Publications. Most Trusted. Most Cited. Most Read
Molecular-Level Dysregulation of Insulin Pathways and Inflammatory Processes in Peripheral Blood Mononuclear Cells by Circadian Misalignment
My Activity

Figure 1Loading Img
    ADDITION/CORRECTION. This article has been corrected. View the notice.
    Article

    Molecular-Level Dysregulation of Insulin Pathways and Inflammatory Processes in Peripheral Blood Mononuclear Cells by Circadian Misalignment
    Click to copy article linkArticle link copied!

    • Jason E. McDermott*
      Jason E. McDermott
      Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States
      Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, United States
      *E-mail: [email protected]. Tel: 509-372-4360.
    • Jon M. Jacobs
      Jon M. Jacobs
      Environmental and Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States
    • Nathaniel J. Merrill
      Nathaniel J. Merrill
      Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States
    • Hugh D. Mitchell
      Hugh D. Mitchell
      Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States
    • Osama A. Arshad
      Osama A. Arshad
      Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States
    • Ryan McClure
      Ryan McClure
      Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States
      More by Ryan McClure
    • Justin Teeguarden
      Justin Teeguarden
      Environmental and Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States
    • Rajendra P. Gajula
      Rajendra P. Gajula
      Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, Washington 99202, United States
    • Kenneth I. Porter
      Kenneth I. Porter
      Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, Washington 99202, United States
    • Brieann C. Satterfield
      Brieann C. Satterfield
      Sleep and Performance Research Center, Washington State University, Spokane, Washington 99202, United States
      Department of Translational Medicine and Physiology, Washington State University, Spokane, Washington 99202, United States
    • Kirsie R. Lundholm
      Kirsie R. Lundholm
      Sleep and Performance Research Center, Washington State University, Spokane, Washington 99202, United States
      Department of Translational Medicine and Physiology, Washington State University, Spokane, Washington 99202, United States
    • Debra J. Skene
      Debra J. Skene
      Faculty of Health and Medical Sciences, University of Surrey, Guildford, GU2 7XH, United Kingdom
    • Shobhan Gaddameedhi
      Shobhan Gaddameedhi
      Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695, United States
      Center for Human Health and the Environment, North Carolina State University, Raleigh, North Carolina 27695, United States
    • H. P. A. Van Dongen
      H. P. A. Van Dongen
      Sleep and Performance Research Center, Washington State University, Spokane, Washington 99202, United States
      Department of Translational Medicine and Physiology, Washington State University, Spokane, Washington 99202, United States
    Other Access OptionsSupporting Information (5)

    Journal of Proteome Research

    Cite this: J. Proteome Res. 2024, 23, 5, 1547–1558
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jproteome.3c00418
    Published April 15, 2024
    Copyright © 2024 American Chemical Society

    Abstract

    Click to copy section linkSection link copied!
    Abstract Image

    Circadian misalignment due to night work has been associated with an elevated risk for chronic diseases. We investigated the effects of circadian misalignment using shotgun protein profiling of peripheral blood mononuclear cells taken from healthy humans during a constant routine protocol, which was conducted immediately after participants had been subjected to a 3-day simulated night shift schedule or a 3-day simulated day shift schedule. By comparing proteomic profiles between the simulated shift conditions, we identified proteins and pathways that are associated with the effects of circadian misalignment and observed that insulin regulation pathways and inflammation-related proteins displayed markedly different temporal patterns after simulated night shift. Further, by integrating the proteomic profiles with previously assessed metabolomic profiles in a network-based approach, we found key associations between circadian dysregulation of protein-level pathways and metabolites of interest in the context of chronic metabolic diseases. Endogenous circadian rhythms in circulating glucose and insulin differed between the simulated shift conditions. Overall, our results suggest that circadian misalignment is associated with a tug of war between central clock mechanisms controlling insulin secretion and peripheral clock mechanisms regulating insulin sensitivity, which may lead to adverse long-term outcomes such as diabetes and obesity. Our study provides a molecular-level mechanism linking circadian misalignment and adverse long-term health consequences of night work.

    Copyright © 2024 American Chemical Society

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Supporting Information

    Click to copy section linkSection link copied!

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jproteome.3c00418

    • Table of Supporting Information content (PDF)

    • Processed proteomics data from MS/MS analysis and results of the cosinor rhythmicity analysis (Table S1) (XLSX)

    • Functional enrichment results from the study (Table S2) (XLSX)

    • Processed data from glucose and insulin measurements and cosinor analysis (Table S3) (XLS)

    • Cosinor analysis of proteomics data for sex differences (Table S4) (XLSX)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    Click to copy section linkSection link copied!
    Citation Statements
    Explore this article's citation statements on scite.ai

    This article is cited by 2 publications.

    1. Matt Spick, Cheryl M. Isherwood, Lee A. Gethings, Christopher J. Hughes, Matthew E. Daly, Hana Hassanin, Daan R. van der Veen, Debra J. Skene, Jonathan D. Johnston. Challenges and opportunities for statistical power and biomarker identification arising from rhythmic variation in proteomics. npj Biological Timing and Sleep 2025, 2 (1) https://doi.org/10.1038/s44323-024-00020-2
    2. Ahmed Arabi, Dima Nasrallah, Sara Mohsen, Lana Abugharbieh, Dana Al-Hashimi, Shaikha AlMass, Shahd Albasti, Saeed A. Al-Ajmi, Muhammad Naseem Khan, Susu M. Zughaier. Association between Serum Vitamin D Status and Circadian Syndrome: A Cross-Sectional Study. Nutrients 2024, 16 (13) , 2111. https://doi.org/10.3390/nu16132111
    3. Matt Spick, Cheryl M Isherwood, Lee Gethings, Hana Hassanin, Daan R van der Veen, Debra J. Skene, Jonathan D Johnston. Rhythmic variation in proteomics: challenges and opportunities for statistical power and biomarker identification. 2024https://doi.org/10.1101/2024.06.28.601121

    Journal of Proteome Research

    Cite this: J. Proteome Res. 2024, 23, 5, 1547–1558
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jproteome.3c00418
    Published April 15, 2024
    Copyright © 2024 American Chemical Society

    Article Views

    1199

    Altmetric

    -

    Citations

    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.