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Distinguish Esophageal Cancer Cells through VOCs Induced by Methionine Regulation

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    Distinguish Esophageal Cancer Cells through VOCs Induced by Methionine Regulation
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    • Jijuan Zhou
      Jijuan Zhou
      Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      University of Science and Technology of China, Hefei 230026, Anhui, China
      More by Jijuan Zhou
    • Dianlong Ge*
      Dianlong Ge
      Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      *Email: [email protected]
      More by Dianlong Ge
    • Yajing Chu
      Yajing Chu
      Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      University of Science and Technology of China, Hefei 230026, Anhui, China
      More by Yajing Chu
    • Yue Liu
      Yue Liu
      Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      University of Science and Technology of China, Hefei 230026, Anhui, China
      More by Yue Liu
    • Yan Lu
      Yan Lu
      Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      More by Yan Lu
    • Yannan Chu*
      Yannan Chu
      Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei 230031, Anhui, China
      *Email: [email protected]
      More by Yannan Chu
      Orcidhttps://orcid.org/0000-0001-7422-905X
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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2024, 23, 7, 2552–2560
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    https://doi.org/10.1021/acs.jproteome.4c00270
    Published June 12, 2024
    Copyright © 2024 American Chemical Society
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    Abstract

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    Detection of exhaled volatile organic compounds (VOCs) is promising for noninvasive screening of esophageal cancer (EC). Cellular VOC analysis can be used to investigate potential biomarkers. Considering the crucial role of methionine (Met) during cancer development, exploring associated abnormal metabolic phenotypes becomes imperative. In this work, we employed headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) to investigate the volatile metabolic profiles of EC cells (KYSE150) and normal esophageal epithelial cells (HEECs) under a Met regulation strategy. Using untargeted approaches, we analyzed the metabolic VOCs of the two cell types and explored the differential VOCs between them. Subsequently, we utilized targeted approaches to analyze the differential VOCs in both cell types under gradient Met culture conditions. The results revealed that there were five/six differential VOCs between cells under Met-containing/Met-free culture conditions. And the difference in levels of two characteristic VOCs (1-butanol and ethyl 2-methylbutyrate) between the two cell types intensified with the increase of the Met concentration. Notably, this is the first report on VOC analysis of EC cells and the first to consider the effect of Met on volatile metabolic profiles. The present work indicates that EC cells can be distinguished through VOCs induced by Met regulation, which holds promise for providing novel insights into diagnostic strategies.

    Copyright © 2024 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jproteome.4c00270.

    • List of studies on exhaled VOCs in esophageal cancer patients (Table S2); detailed information on all of the identified VOCs (Table S1); cell viability of KYSE150 and HEEC under gradient Met concentration culture conditions (Figure S1); optimization of SPME conditions (Figure S2); GC–MS method validation results (Figure S3); chromatogram of VOCs qualitatively identified by standard reagents (Figure S4); peak areas of nine differential VOCs under EMEM/EMEM-Met culture conditions (Figure S5) (PDF)

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    This article is cited by 2 publications.

    1. Jijuan Zhou, Dianlong Ge, Yue Liu, Yajing Chu, Xiangxue Zheng, Yan Lu, Yannan Chu. Developing Multiple Media Approach to Investigate Reproducible Characteristic VOCs of Lung Cancer Cells. Analytical Chemistry 2024, 96 (52) , 20398-20405. https://doi.org/10.1021/acs.analchem.4c03894
    2. Dushyant Singh Dahiya, Sheza Malik, Ruchir Paladiya, Sidra Ahsan, Haniya Wasim, Hareesha Rishab Bharadwaj, Abhishek Goel, Ali Jaan, Umar Hayat, Fariha Hasan, Sneh Sonaiya, Hassam Ali. Advances in Non-Invasive Screening Methods for Gastrointestinal Cancers: How Continued Innovation Has Revolutionized Early Cancer Detection. Cancers 2025, 17 (7) , 1085. https://doi.org/10.3390/cancers17071085
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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2024, 23, 7, 2552–2560
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jproteome.4c00270
    Published June 12, 2024
    Copyright © 2024 American Chemical Society
    Request reuse permissions

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