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Proteome-wide Adaptations of Mouse Skeletal Muscles during a Full Month in Space

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Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Proteome Res. 2017, 16, 7, 2623-2638
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    Proteome-wide Adaptations of Mouse Skeletal Muscles during a Full Month in Space
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    † Université de Strasbourg, CNRS, IPHC UMR 7178, F-670000 Strasbourg, France
    ‡ Centre National d’Etudes Spatiales, CNES, 75039 Paris, France
    § Université de Montpellier, INRA, UMR 866 Dynamique Musculaire et Métabolisme, Montpellier F-34060, France
    ∥ National Institute for Cellular Biotechnology and the School of Health and Human Performance, Dublin City University, Dublin 9, Ireland
    *Tel: +33 3 68852681. E-mail: [email protected]
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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2017, 16, 7, 2623–2638
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    https://doi.org/10.1021/acs.jproteome.7b00201
    Published June 7, 2017
    Copyright © 2017 American Chemical Society
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    Abstract

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    The safety of space flight is challenged by a severe loss of skeletal muscle mass, strength, and endurance that may compromise the health and performance of astronauts. The molecular mechanisms underpinning muscle atrophy and decreased performance have been studied mostly after short duration flights and are still not fully elucidated. By deciphering the muscle proteome changes elicited in mice after a full month aboard the BION-M1 biosatellite, we observed that the antigravity soleus incurred the greatest changes compared with locomotor muscles. Proteomics data notably suggested mitochondrial dysfunction, metabolic and fiber type switching toward glycolytic type II fibers, structural alterations, and calcium signaling-related defects to be the main causes for decreased muscle performance in flown mice. Alterations of the protein balance, mTOR pathway, myogenesis, and apoptosis were expected to contribute to muscle atrophy. Moreover, several signs reflecting alteration of telomere maintenance, oxidative stress, and insulin resistance were found as possible additional deleterious effects. Finally, 8 days of recovery post flight were not sufficient to restore completely flight-induced changes. Thus in-depth proteomics analysis unraveled the complex and multifactorial remodeling of skeletal muscle structure and function during long-term space flight, which should help define combined sets of countermeasures before, during, and after the flight.

    Copyright © 2017 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jproteome.7b00201.

    • Table S-7. Comparison of the effects of actual and simulated microgravity on skeletal muscles. Supplementary Figure S-1. Overview of vastus lateralis regulated proteins in response to spaceflight in Bion-M1 mice. Figure S-2. Annotated MS/MS spectra for all single peptide-based protein identifications in the EDL, soleus, and vastus lateralis muscles of Bion M1 mice. (PDF)

    • Table S-1. List of analyzed proteins in the EDL, soleus, and vastus lateralis muscles of Bion M1 mice, their annotations, and statistical analysis. (XLSX)

    • Table S-2. Significantly enriched GO terms from differential muscle proteins in flown Bion-M1 mice (Flight group; F) compared to the Ground Control (GC) and Vivarium Control (VC) animals. (XLSX)

    • Table S-3. Significantly enriched GO terms from down- and up-regulated muscle proteins in flown Bion-M1 mice (Flight group) compared to both the Ground Control and Vivarium Control animals. (XLSX)

    • Table S-4. Significantly down- and up-regulated muscle proteins in flown Bion-M1 mice (Flight group) compared to the Ground Control (GC) and Vivarium Control (VC) groups. (XLSX)

    • Table S-5. Overview of recovery-induced restoration of protein abundances in Bion-M1 mice. (XLSX)

    • Table S-6. List of single peptide-based protein identifications in the EDL, soleus, and vastus lateralis muscles of Bion M1 mice and their annotations. (XLSX)

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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2017, 16, 7, 2623–2638
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jproteome.7b00201
    Published June 7, 2017
    Copyright © 2017 American Chemical Society
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