ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Figure 1Loading Img

Proteomic Profile of a Chronic Binge Ethanol Exposure Model

  • Phillip Starski
    Phillip Starski
    Neuroscience Program, Mayo Clinic, Rochester, Minnesota 55905, United States
  • Lee Peyton
    Lee Peyton
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota 55905, United States
    More by Lee Peyton
  • Alfredo Oliveros
    Alfredo Oliveros
    Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States
  • Carrie J. Heppelmann
    Carrie J. Heppelmann
    Proteomics Research Center, Mayo Clinic, Rochester, Minnesota 55905, United States
  • Surendra Dasari
    Surendra Dasari
    Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota 55905, United States
  • , and 
  • Doo-Sup Choi*
    Doo-Sup Choi
    Neuroscience Program,  Department of Molecular Pharmacology and Experimental Therapeutics  and  Department of Psychiatry and Psychology, Mayo Clinic, Rochester, Minnesota 55905, United States
    *Tel: 507-284-5602. Fax: 507-284-1762. E-mail: [email protected]
    More by Doo-Sup Choi
Cite this: J. Proteome Res. 2019, 18, 9, 3492–3502
Publication Date (Web):July 22, 2019
https://doi.org/10.1021/acs.jproteome.9b00394
Copyright © 2019 American Chemical Society

    Article Views

    809

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (1)»

    Abstract

    Abstract Image

    Chronic binge alcohol drinking is known to increase risky decision through pathological impulsive behaviors. Recently, we established a novel rodent model of ethanol-induced waiting impulsivity using 5-choice serial reaction time task (5-CSRTT) in mice. However, molecular mechanisms underlying the chronic binge ethanol-induced waiting impulsivity is not well characterized. Among brain regions involved in impulsivity, the anterior cingulate cortex (ACC) is a major neural substrate for mediating the 5-CSRTT-based waiting impulsivity. Thus, we sought to determine the ACC proteomic profile using label-free proteomics of mice exhibiting ethanol-induced impulsivity. Ingenuity pathway analysis revealed that impulsivity-related proteins involved in ion channel complexes such as KCNIP3 (potassium voltage-gated channel interacting protein 3) and CACNG2 (calcium voltage-gated channel auxiliary subunit gamma 2) are downregulated in the ACC. We identified significant protein expression changes in the mechanistic target of rapamycin (mTOR) canonical pathway between control and ethanol-induced impulsive mice. Impulsive mice showed over 60% of proteins involved in the mTOR canonical pathway have been altered. This pathway has been previously implicated in the neuroadaptation in drugs of abuse and impulsivity. We found substantial changes in the protein levels involved in neurological disorders such as schizophrenia and Alzheimer’s disease. Our findings provide a neuroproteomic profile of ethanol-induced impulsive mice.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jproteome.9b00394.

    • Behavioral data of the entire cohort; behavioral data of the mice used for proteomic analysis; mass spectrometry quality control; top upregulated proteins; top downregulated proteins (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 8 publications.

    1. Ada Man-Choi Ho, Mina P. Peyton, Samantha J. Scaletty, Sarah Trapp, Areonna Schreiber, Benjamin J. Madden, Doo-Sup Choi, Douglas B. Matthews. Chronic Intermittent Ethanol Exposure Alters Behavioral Flexibility in Aged Rats Compared to Adult Rats and Modifies Protein and Protein Pathways Related to Alzheimer’s Disease. ACS Omega 2022, 7 (50) , 46260-46276. https://doi.org/10.1021/acsomega.2c04528
    2. Phillip Starski, Addyson Siegle, F. Woodward Hopf. Engagement for alcohol escalates in the 5-choice serial reaction time task after intermittent access. Alcohol 2024, 115 , 79-92. https://doi.org/10.1016/j.alcohol.2024.01.004
    3. Yingping Ma, Shaofei Jiang, Xin Zhao, Shen Li, Liping Chen, Zhe Zhao, Wei Shen, Yan Wu, Haitao Wu. CaMKIIα neurons in the anterior insular cortex regulate attention behavior in mice. Frontiers in Neural Circuits 2023, 17 https://doi.org/10.3389/fncir.2023.1197541
    4. Phillip Starski, Danielle Maulucci, Hunter Mead, Frederic Hopf. Adaptation of the 5-choice serial reaction time task to measure engagement and motivation for alcohol in mice. Frontiers in Behavioral Neuroscience 2022, 16 https://doi.org/10.3389/fnbeh.2022.968359
    5. Marcos Ucha, David Roura-Martínez, Emilio Ambrosio, Alejandro Higuera-Matas. The role of the mTOR pathway in models of drug-induced reward and the behavioural constituents of addiction. Journal of Psychopharmacology 2020, 34 (11) , 1176-1199. https://doi.org/10.1177/0269881120944159
    6. Pei Shang, Daniel Lindberg, Phillip Starski, Lee Peyton, Sa-Ik Hong, Sun Choi, Doo-Sup Choi. Chronic Alcohol Exposure Induces Aberrant Mitochondrial Morphology and Inhibits Respiratory Capacity in the Medial Prefrontal Cortex of Mice. Frontiers in Neuroscience 2020, 14 https://doi.org/10.3389/fnins.2020.561173
    7. Yan Zhou, Yupu Liang, Mary Jeanne Kreek. mTORC1 pathway is involved in the kappa opioid receptor activation-induced increase in excessive alcohol drinking in mice. Pharmacology Biochemistry and Behavior 2020, 195 , 172954. https://doi.org/10.1016/j.pbb.2020.172954
    8. Reginald Cannady, Sudarat Nimitvilai-Roberts, Sarah D. Jennings, John J. Woodward, Patrick J. Mulholland. Distinct Region- and Time-Dependent Functional Cortical Adaptations in C57BL/6J Mice after Short and Prolonged Alcohol Drinking. eneuro 2020, 7 (3) , ENEURO.0077-20.2020. https://doi.org/10.1523/ENEURO.0077-20.2020

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect