ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Organized Hybrid Molecular Films from Natural Phospholipids and Synthetic Block Copolymers: A Physicochemical Investigation

Cite this: Langmuir 2020, 36, 37, 10941–10951
Publication Date (Web):August 27, 2020
https://doi.org/10.1021/acs.langmuir.0c01544
Copyright © 2020 American Chemical Society

    Article Views

    892

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (1)»

    Abstract

    Abstract Image

    In the last few years, hybrid lipid-copolymer assemblies have attracted increasing attention as possible two-dimensional (2D) membrane platforms, combining the biorelevance of the lipid building blocks with the stability and chemical tunability of copolymers. The relevance of these systems varies from fundamental studies on biological membrane-related phenomena to the construction of 2D complex devices for material science and biosensor technology. Both the fundamental understanding and the application of hybrid lipid-copolymer-supported bilayers require thorough physicochemical comprehension and structural control. Herein, we report a comprehensive physicochemical and structural characterization of hybrid monolayers at the air/water interface and of solid-supported hybrid membranes constituted by 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and the block copolymer poly(butadiene-b-ethyleneoxide) (PBD-b-PEO). Hybrid lipid-copolymer supported bilayers (HSLBs) with variable copolymer contents were prepared through spontaneous rupture and fusion of hybrid vesicles onto a hydrophilic substrate. The properties of the thin films and the parent vesicles were probed through dynamic light scattering (DLS), differential scanning calorimetry (DSC), optical ellipsometry, quartz crystal microbalance with dissipation monitoring (QCM-D), and confocal scanning laser microscopy (CSLM). Stable, hybrid lipid/copolymer systems were obtained for a copolymer content of 10–65 mol %. In particular, DSC and CSLM show lateral phase separation in these hybrid systems. These results improve our fundamental understanding of HSLBs, which is necessary for future applications of hybrid systems as biomimetic membranes or as drug delivery systems, with additional properties with respect to phospholipid liposomes.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.langmuir.0c01544.

    • Histograms of the intensity distribution for vesicles in water; derivation of eq 4; complete Π–A isothermal cycles; surface compressive modulus for the complete isothermal cycles; refractive index increments for pure DPPC and PBD-b-PEO solutions; ellipsometric studies for all of the monolayers; QCM-D-sensed mass for the supported bilayers; ellipsometric studies for all of the supported bilayers; and CSLM images for pure DPPC and PBD-b-PEO bilayers (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 9 publications.

    1. Samantha Micciulla, Philipp Gutfreund, Matej Kanduč, Leonardo Chiappisi. Pressure-Induced Phase Transitions of Nonionic Polymer Brushes. Macromolecules 2023, 56 (3) , 1177-1188. https://doi.org/10.1021/acs.macromol.2c01979
    2. Yoo Kyung Go, Cecilia Leal. Polymer–Lipid Hybrid Materials. Chemical Reviews 2021, 121 (22) , 13996-14030. https://doi.org/10.1021/acs.chemrev.1c00755
    3. Efstathia Triantafyllopoulou, Dimitrios Selianitis, Anastasia Balafouti, Nefeli Lagopati, Maria Gazouli, Georgia Valsami, Stergios Pispas, Natassa Pippa. Fabricating hybrid DSPC:DOPC:P(OEGMA-co-LMA) structures: Self-assembly as the milestone of their performance. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2024, 684 , 133015. https://doi.org/10.1016/j.colsurfa.2023.133015
    4. Jacopo Cardellini, Arianna Balestri, Luca Comparini, Barbara Lonetti, Marco Brucale, Francesco Valle, Debora Berti, Costanza Montis. Controlling plasmonic suprastructures through self-assembly of gold nanoparticles with hybrid copolymer-lipid vesicles. Journal of Colloid and Interface Science 2024, 654 , 848-858. https://doi.org/10.1016/j.jcis.2023.10.082
    5. Rashmi Seneviratne, Georgina Coates, Zexi Xu, Caitlin E. Cornell, Rebecca F. Thompson, Amin Sadeghpour, Daniel P. Maskell, Lars J. C. Jeuken, Michael Rappolt, Paul A. Beales. High Resolution Membrane Structures within Hybrid Lipid‐Polymer Vesicles Revealed by Combining X‐Ray Scattering and Electron Microscopy. Small 2023, 19 (22) https://doi.org/10.1002/smll.202206267
    6. Arianna Balestri, Barbara Lonetti, Simon Harrisson, Barbara Farias-Mancilla, Junliang Zhang, Heinz Amenitsch, Ulrich S. Schubert, Carlos Guerrero-Sanchez, Costanza Montis, Debora Berti. Thermo-responsive lipophilic NIPAM-based block copolymers as stabilizers for lipid-based cubic nanoparticles. Colloids and Surfaces B: Biointerfaces 2022, 220 , 112884. https://doi.org/10.1016/j.colsurfb.2022.112884
    7. Maryame Bina, Flavien Sciortino, Agata N. Mahrir. Phase separation in polymer-based biomimetic structures containing planar membranes. Biointerphases 2022, 17 (6) , 060802. https://doi.org/10.1116/6.0002078
    8. Subhadeep Koner, Joseph Tawfik, Farzin Mashali, Kristen B. Kennison, William T. McClintic, Frederick A. Heberle, Yu-Ming Tu, Manish Kumar, Stephen A. Sarles. Homogeneous hybrid droplet interface bilayers assembled from binary mixtures of DPhPC phospholipids and PB-b-PEO diblock copolymers. Biochimica et Biophysica Acta (BBA) - Biomembranes 2022, 1864 (10) , 183997. https://doi.org/10.1016/j.bbamem.2022.183997
    9. Jacopo Cardellini, Arianna Balestri, Costanza Montis, Debora Berti. Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems. Pharmaceutics 2021, 13 (6) , 861. https://doi.org/10.3390/pharmaceutics13060861

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect