Organized Hybrid Molecular Films from Natural Phospholipids and Synthetic Block Copolymers: A Physicochemical InvestigationClick to copy article linkArticle link copied!
- Arianna BalestriArianna BalestriDepartment of Chemistry “Ugo Schiff” and CSGI, via della Lastruccia 3-13, 50019 Florence, ItalyInstitut Laue Langevin, 71 avenue des Martyrs, 38000 Grenoble, FranceMore by Arianna Balestri
- Leonardo Chiappisi*Leonardo Chiappisi*Email: [email protected]Institut Laue Langevin, 71 avenue des Martyrs, 38000 Grenoble, FranceMore by Leonardo Chiappisi
- Costanza MontisCostanza MontisDepartment of Chemistry “Ugo Schiff” and CSGI, via della Lastruccia 3-13, 50019 Florence, ItalyMore by Costanza Montis
- Samantha MicciullaSamantha MicciullaInstitut Laue Langevin, 71 avenue des Martyrs, 38000 Grenoble, FranceMore by Samantha Micciulla
- Barbara Lonetti*Barbara Lonetti*Email: [email protected]Laboratoire des IMRCP, Université de Toulouse, CNRS UMR 5623, Université Toulouse III- Paul Sabatier, 118 route de Narbonne, F-31062 Toulouse, Cedex 9, FranceMore by Barbara Lonetti
- Debora BertiDebora BertiDepartment of Chemistry “Ugo Schiff” and CSGI, via della Lastruccia 3-13, 50019 Florence, ItalyMore by Debora Berti
Abstract
In the last few years, hybrid lipid-copolymer assemblies have attracted increasing attention as possible two-dimensional (2D) membrane platforms, combining the biorelevance of the lipid building blocks with the stability and chemical tunability of copolymers. The relevance of these systems varies from fundamental studies on biological membrane-related phenomena to the construction of 2D complex devices for material science and biosensor technology. Both the fundamental understanding and the application of hybrid lipid-copolymer-supported bilayers require thorough physicochemical comprehension and structural control. Herein, we report a comprehensive physicochemical and structural characterization of hybrid monolayers at the air/water interface and of solid-supported hybrid membranes constituted by 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and the block copolymer poly(butadiene-b-ethyleneoxide) (PBD-b-PEO). Hybrid lipid-copolymer supported bilayers (HSLBs) with variable copolymer contents were prepared through spontaneous rupture and fusion of hybrid vesicles onto a hydrophilic substrate. The properties of the thin films and the parent vesicles were probed through dynamic light scattering (DLS), differential scanning calorimetry (DSC), optical ellipsometry, quartz crystal microbalance with dissipation monitoring (QCM-D), and confocal scanning laser microscopy (CSLM). Stable, hybrid lipid/copolymer systems were obtained for a copolymer content of 10–65 mol %. In particular, DSC and CSLM show lateral phase separation in these hybrid systems. These results improve our fundamental understanding of HSLBs, which is necessary for future applications of hybrid systems as biomimetic membranes or as drug delivery systems, with additional properties with respect to phospholipid liposomes.
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This article is cited by 11 publications.
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