ACS Publications. Most Trusted. Most Cited. Most Read
Rapid and Sensitive Detection of Mutations in SARS-CoV-2 by Surface-Enhanced Raman Spectroscopy
My Activity
    Article

    Rapid and Sensitive Detection of Mutations in SARS-CoV-2 by Surface-Enhanced Raman Spectroscopy
    Click to copy article linkArticle link copied!

    • Liping Chen
      Liping Chen
      School of Science, Xihua University, Chengdu 610039, PR China
      More by Liping Chen
    • Xuanjiang Zeng
      Xuanjiang Zeng
      School of Science, Xihua University, Chengdu 610039, PR China
    • Fan Yang
      Fan Yang
      School of Science, Xihua University, Chengdu 610039, PR China
      More by Fan Yang
    • Tao Yang
      Tao Yang
      School of Science, Xihua University, Chengdu 610039, PR China
      More by Tao Yang
    • Yushi Chen
      Yushi Chen
      School of Science, Xihua University, Chengdu 610039, PR China
      More by Yushi Chen
    • Yan Zhao*
      Yan Zhao
      School of Science, Xihua University, Chengdu 610039, PR China
      *Email: [email protected]
      More by Yan Zhao
    • Xiaojun Luo*
      Xiaojun Luo
      School of Science, Xihua University, Chengdu 610039, PR China
      *Email: [email protected]
      More by Xiaojun Luo
    • Yuanyuan Li*
      Yuanyuan Li
      Shanghai Institute of Doping Analyses, Shanghai University of Sport, Shanghai 200438, PR China
      *Email: [email protected]
      More by Yuanyuan Li
    Other Access OptionsSupporting Information (1)

    Langmuir

    Cite this: Langmuir 2025, 41, 5, 3583–3591
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.langmuir.4c04725
    Published January 30, 2025
    Copyright © 2025 American Chemical Society

    Abstract

    Click to copy section linkSection link copied!
    Abstract Image

    Since the outbreak of the novel coronavirus (SARS-CoV-2), the world has suffered significant losses. At present, the pneumonia disease caused by SARS-CoV-2 virus has not been eliminated, and SARS-CoV-2 has a high mutation rate, and its variant strains also have a high prevalence rate, which has always threatened the health of all mankind. This study aims to develop a rapid and sensitive method to complement existing SARS-CoV-2 diagnostic tools by utilizing surface-enhanced Raman spectroscopy (SERS) for the direct detection of the intrinsic SERS signal from the S proteins of SARS-CoV-2 and its variants (Omicron and Delta) within 5 min using a portable Raman spectrometer. The linear range of S protein detection of Wild-type, Omicron and Delta variants ranged from 1.0 × 10–7 to 1.0 × 10–3 g·mL–1, the limits of detection (LOD) were down to ∼10–8 g·mL–1 level. Our proposed method uses portable Raman spectrometer for direct detection, which is characterized by its simplicity, rapidity, portability, and wide applicability. It enables simultaneous detection of diverse mutated targets, thereby playing a pivotal role in the early diagnosis of viral diseases. Moreover, it holds promising prospects in the fields of chemistry, biology, and medicine.

    Copyright © 2025 American Chemical Society

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Supporting Information

    Click to copy section linkSection link copied!

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.langmuir.4c04725.

    • Calculation of enhancement factor (EF), Au NS synthesized under different heating time and corresponding UV–vis spectra, Raman bands of Wild-type, Omicron and Delta variants virus S proteins and their attribution, evaluation of several techniques for detecting SARS-CoV-2 and its variant S protein (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    Click to copy section linkSection link copied!

    This article has not yet been cited by other publications.

    Langmuir

    Cite this: Langmuir 2025, 41, 5, 3583–3591
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.langmuir.4c04725
    Published January 30, 2025
    Copyright © 2025 American Chemical Society

    Article Views

    104

    Altmetric

    -

    Citations

    -
    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.