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Nanoroughness Strongly Impacts Lipid Mobility in Supported Membranes

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Université Lyon, Université Claude Bernard Lyon 1, CNRS, Institut Lumière Matière, F-69622 Villeurbanne, France
Doctoral School for Science and Technology, Platform for Research in NanoSciences and Nanotechnology, Campus Pierre Gemayel, Lebanese University, Fanar-Metn BP 90239 Beirut, Lebanon
§ CNRS, INSA de Lyon, LaMCoS, UMR5259, Université de Lyon, 69621 Lyon, France
Université de Strasbourg, Institut Charles Sadron, UPR22, CNRS, 67034 Strasbourg Cedex 2, France
Institut Laue-Langevin, 71 Avenue des Martyrs, F-38042 Grenoble, France
# CINaM-CNRS, Aix-Marseille Université, UMR7325, 13288 Marseille, France
Cite this: Langmuir 2017, 33, 9, 2444–2453
Publication Date (Web):February 20, 2017
https://doi.org/10.1021/acs.langmuir.6b03276
Copyright © 2017 American Chemical Society

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    Abstract

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    In vivo lipid membranes interact with rough supramolecular structures such as protein clusters and fibrils. How these features whose size ranges from a few nanometers to a few tens of nanometers impact lipid and protein mobility is still being investigated. Here, we study supported phospholipid bilayers, a unique biomimetic model, deposited on etched surfaces bearing nanometric corrugations. The surface roughness and mean curvature are carefully characterized by AFM imaging using ultrasharp tips. Neutron specular reflectivity supplements this surface characterization and indicates that the bilayers follow the large-scale corrugations of the substrate. We measure the lateral mobility of lipids in both the fluid and gel phases by fluorescence recovery after patterned photobleaching. Although the mobility is independent of the roughness in the gel phase, it exhibits a 5-fold decrease in the fluid phase when the roughness increases from 0.2 to 10 nm. These results are interpreted with a two-phase model allowing for a strong decrease in the lipid mobility in highly curved or defect-induced gel-like nanoscale regions. This suggests a strong link between membrane curvature and fluidity, which is a key property for various cell functions such as signaling and adhesion.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.langmuir.6b03276.

    • Local unbinding of lipid membranes on rough substrates: models and discussion. Bare surface characterization and comparison of images with or without SPBs: complementary AFM analysis. Neutron reflectivity: specular reflectivity curves and fitted SLD. Fluorescence recovery after patterned photobleaching: typical curves. (PDF)

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