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Biomimetic Planar Polymer Membranes Decorated with Enzymes as Functional Surfaces

  • Camelia Draghici
    Camelia Draghici
    Chemistry Department, University of Basel, Mattenstrasse 24a, BPR 1096, 4002 Basel, Switzerland
    Product Design, Mechatronics and Environment Department, Transilvania University of Brasov, 29 Eroilor Blv, 500036 Brasov, Romania
  • Viktoria Mikhalevich
    Viktoria Mikhalevich
    Chemistry Department, University of Basel, Mattenstrasse 24a, BPR 1096, 4002 Basel, Switzerland
  • Gesine Gunkel-Grabole
    Gesine Gunkel-Grabole
    Chemistry Department, University of Basel, Mattenstrasse 24a, BPR 1096, 4002 Basel, Switzerland
  • Justyna Kowal
    Justyna Kowal
    Chemistry Department, University of Basel, Mattenstrasse 24a, BPR 1096, 4002 Basel, Switzerland
  • Wolfgang Meier
    Wolfgang Meier
    Chemistry Department, University of Basel, Mattenstrasse 24a, BPR 1096, 4002 Basel, Switzerland
  • , and 
  • Cornelia G. Palivan*
    Cornelia G. Palivan
    Chemistry Department, University of Basel, Mattenstrasse 24a, BPR 1096, 4002 Basel, Switzerland
    *E-mail: [email protected]
Cite this: Langmuir 2018, 34, 30, 9015–9024
Publication Date (Web):July 4, 2018
https://doi.org/10.1021/acs.langmuir.8b00541
Copyright © 2018 American Chemical Society
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Abstract

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Functional surfaces were generated by a combination of enzymes with polymer membranes composed of an amphiphilic, asymmetric block copolymer poly(ethyleneglycol)-block-poly(γ-methyl-ε-caprolactone)-block-poly[(2-dimethylamino)ethylmethacrylate]. First, polymer films formed at the air–water interface were transferred in different sequences onto silica solid support using the Langmuir–Blodgett technique, generating homogeneous monolayers and bilayers. A detailed characterization of these films provided insight into their properties (film thickness, wettability, topography, and roughness). On the basis of these findings, the most promising membranes were selected for enzyme attachment. Functional surfaces were then generated by the adsorption of two model enzymes that can convert phenol and its derivatives (laccase and tyrosinase), well known as high-risk pollutants of drinking and natural water. Both enzymes preserved their activity upon immobilization with respect to their substrates. Depending on the properties of the polymer films, different degrees of enzymatic activity were observed: bilayers provided the best conditions in terms of both overall stability and enzymatic activity. The interaction between amphiphilic triblock copolymer films and enzymes is exploited to engineer “active surfaces” with specific functionalities and high efficacy resulting from the intrinsic activity of the biomolecules that is preserved by an appropriate synthetic environment.

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The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.langmuir.8b00541.

  • Characterization of the polymer films; AFM; roughness of synthetic films; QCM-D frequency changes; adsorption of enzymes on synthetic films; activity of the free laccase and tyrosinase; absorption curves for tyrosinase and laccase on the bilayer; calibration curves for the enzymatic product; activity of the enzymes on the bilayer two weeks after preparation; and relative enzymatic activity (PDF)

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Cited By


This article is cited by 10 publications.

  1. Stefano Di Leone, Jaicy Vallapurackal, Saziye Yorulmaz Avsar, Myrto Kyropolou, Thomas R. Ward, Cornelia G. Palivan, Wolfgang Meier. Expanding the Potential of the Solvent-Assisted Method to Create Bio-Interfaces from Amphiphilic Block Copolymers. Biomacromolecules 2021, 22 (7) , 3005-3016. https://doi.org/10.1021/acs.biomac.1c00424
  2. Stefano Di Leone, Saziye Yorulmaz Avsar, Andrea Belluati, Riccardo Wehr, Cornelia G. Palivan, Wolfgang Meier. Polymer–Lipid Hybrid Membranes as a Model Platform to Drive Membrane–Cytochrome c Interaction and Peroxidase-like Activity. The Journal of Physical Chemistry B 2020, 124 (22) , 4454-4465. https://doi.org/10.1021/acs.jpcb.0c02727
  3. Andrea Belluati, Viktoria Mikhalevich, Saziye Yorulmaz Avsar, Davy Daubian, Ioana Craciun, Mohamed Chami, Wolfgang P. Meier, Cornelia G. Palivan. How Do the Properties of Amphiphilic Polymer Membranes Influence the Functional Insertion of Peptide Pores?. Biomacromolecules 2020, 21 (2) , 701-715. https://doi.org/10.1021/acs.biomac.9b01416
  4. Luisa Zartner, Viviana Maffeis, Cora-Ann Schoenenberger, Ionel Adrian Dinu, Cornelia G. Palivan. Membrane protein channels equipped with a cleavable linker for inducing catalysis inside nanocompartments. Journal of Materials Chemistry B 2021, 9 (43) , 9012-9022. https://doi.org/10.1039/D1TB01463C
  5. Myrto Kyropoulou, Saziye Yorulmaz Avsar, Cora-Ann Schoenenberger, Cornelia G. Palivan, Wolfgang. P. Meier. From spherical compartments to polymer films: exploiting vesicle fusion to generate solid supported thin polymer membranes. Nanoscale 2021, 13 (14) , 6944-6952. https://doi.org/10.1039/D1NR01122G
  6. Saziye Yorulmaz Avsar, Larisa E. Kapinos, Cora-Ann Schoenenberger, Gebhard F. X. Schertler, Jonas Mühle, Benoit Meger, Roderick Y. H. Lim, Martin K. Ostermaier, Elena Lesca, Cornelia G. Palivan. Immobilization of arrestin-3 on different biosensor platforms for evaluating GPCR binding. Physical Chemistry Chemical Physics 2020, 22 (41) , 24086-24096. https://doi.org/10.1039/D0CP01464H
  7. Agata Krywko-Cendrowska, Stefano di Leone, Maryame Bina, Saziye Yorulmaz-Avsar, Cornelia G. Palivan, Wolfgang Meier. Recent Advances in Hybrid Biomimetic Polymer-Based Films: from Assembly to Applications. Polymers 2020, 12 (5) , 1003. https://doi.org/10.3390/polym12051003
  8. Vittoria Chimisso, Viviana Maffeis, Dimitri Hürlimann, Cornelia G. Palivan, Wolfgang Meier. Self‐Assembled Polymeric Membranes and Nanoassemblies on Surfaces: Preparation, Characterization, and Current Applications. Macromolecular Bioscience 2020, 20 (1) , 1900257. https://doi.org/10.1002/mabi.201900257
  9. Martina Garni, Riccardo Wehr, Saziye Yorulmaz Avsar, Christoph John, Cornelia Palivan, Wolfgang Meier. Polymer membranes as templates for bio-applications ranging from artificial cells to active surfaces. European Polymer Journal 2019, 112 , 346-364. https://doi.org/10.1016/j.eurpolymj.2018.12.047
  10. Saziye Yorulmaz Avsar, Myrto Kyropoulou, Stefano Di Leone, Cora-Ann Schoenenberger, Wolfgang P. Meier, Cornelia G. Palivan. Biomolecules Turn Self-Assembling Amphiphilic Block Co-polymer Platforms Into Biomimetic Interfaces. Frontiers in Chemistry 2019, 6 https://doi.org/10.3389/fchem.2018.00645

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