ACS Publications. Most Trusted. Most Cited. Most Read
My Activity

Rapid Mussel-Inspired Surface Zwitteration for Enhanced Antifouling and Antibacterial Properties

  • Anika Benozir Asha
    Anika Benozir Asha
    Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada
  • Yangjun Chen
    Yangjun Chen
    Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada
    School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China
    More by Yangjun Chen
  • Huixin Zhang
    Huixin Zhang
    Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada
    More by Huixin Zhang
  • Sina Ghaemi
    Sina Ghaemi
    Department of Mechanical Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada
    More by Sina Ghaemi
  • Kazuhiko Ishihara
    Kazuhiko Ishihara
    Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
  • Yang Liu
    Yang Liu
    Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada
    More by Yang Liu
  • , and 
  • Ravin Narain*
    Ravin Narain
    Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 2G6, Canada
    *E-mail: [email protected]. Phone: +1 780 492 1736.
    More by Ravin Narain
Cite this: Langmuir 2019, 35, 5, 1621–1630
Publication Date (Web):December 18, 2018
Copyright © 2018 American Chemical Society

    Article Views





    Other access options
    Supporting Info (1)»


    Abstract Image

    Mussel-inspired dopamine chemistry has increasingly been used for surface modification due to its simplicity, versatility, and strong reactivity for secondary functionalization with amine or thiol containing molecules. In this work, we demonstrate a facile surface modification technique using dopamine chemistry to prepare a zwitterionic polymer coating with both antifouling and antimicrobial property. Catechol containing adhesive monomer dopamine methacrylamide (DMA) was copolymerized with bioinspired zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) monomer, and the synthesized copolymers were covalently grafted onto the amino (−NH2) rich polyethylenimine (PEI)/polydopamine (PDA) codeposited surface to obtain a stable antifouling surface. The resulting surface was later used for in situ deposition of antimicrobial silver nanoparticles (AgNPs), facilitated by the presence of catechol groups of the coating. The modified surface was characterized using X-ray photoelectron spectroscopy (XPS), water contact angle measurements, and atomic force microscopy (AFM). This dual functional coating significantly reduced the adhesion of both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria and showed excellent resistance to bovine serum albumin (BSA) adsorption. This bioinspired and efficient surface modification strategy with dual functional coating promises its potential application in implantable biomedical devices.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.


    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    Jump To

    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.langmuir.8b03810.

    • 1H NMR spectra of dopamine methacrylamide (DMA) and other synthesized polymers and atomic ratio (X/C) elemental analysis of the high resolution XPS and EDX data for elemental compositions of the AgNP loaded surfaces (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system:

    Cited By

    This article is cited by 61 publications.

    1. Ming-Yen Tsai, Ming-Chen Chang, Hsiu-Wen Chien. Effect of Codeposition of Polydopamine with Polyethylenimine or Poly(ethylene glycol) Coatings on Silver Nanoparticle Synthesis. Langmuir 2023, 39 (19) , 6895-6904.
    2. Anika B. Asha, Yi-Yang Peng, Qiuli Cheng, Kazuhiko Ishihara, Yang Liu, Ravin Narain. Dopamine Assisted Self-Cleaning, Antifouling, and Antibacterial Coating via Dynamic Covalent Interactions. ACS Applied Materials & Interfaces 2022, 14 (7) , 9557-9569.
    3. Zhiyun Dong, Xiang Ke, Shuxian Tang, Shuai Wu, Wei Wu, Xingyu Chen, Jiaojiao Yang, Jing Xie, Jun Luo, Jianshu Li. A Stable Cell Membrane-Based Coating with Antibiofouling and Macrophage Immunoregulatory Properties for Implants at the Macroscopic Level. Chemistry of Materials 2021, 33 (20) , 7994-8006.
    4. Elif Nur Durmaz, Sevil Sahin, Ettore Virga, Sissi de Beer, Louis C. P. M. de Smet, Wiebe M. de Vos. Polyelectrolytes as Building Blocks for Next-Generation Membranes with Advanced Functionalities. ACS Applied Polymer Materials 2021, 3 (9) , 4347-4374.
    5. Wenda Wang, Zicheng Zeng, Li Xiang, Cong Liu, Diana Diaz-Dussan, Zunguo Du, Anika B. Asha, Wenshuai Yang, Yi-Yang Peng, Mingfei Pan, Ravin Narain, Jifang Liu, Hongbo Zeng. Injectable Self-Healing Hydrogel via Biological Environment-Adaptive Supramolecular Assembly for Gastric Perforation Healing. ACS Nano 2021, 15 (6) , 9913-9923.
    6. Debirupa Mitra, En-Tang Kang, Koon Gee Neoh. Polymer-Based Coatings with Integrated Antifouling and Bactericidal Properties for Targeted Biomedical Applications. ACS Applied Polymer Materials 2021, 3 (5) , 2233-2263.
    7. Carly Deusenbery, Yingying Wang, Anita Shukla. Recent Innovations in Bacterial Infection Detection and Treatment. ACS Infectious Diseases 2021, 7 (4) , 695-720.
    8. Qiuli Cheng, Anika Benozir Asha, Yang Liu, Yi-Yang Peng, Diana Diaz-Dussan, Zuosen Shi, Zhanchen Cui, Ravin Narain. Antifouling and Antibacterial Polymer-Coated Surfaces Based on the Combined Effect of Zwitterions and the Natural Borneol. ACS Applied Materials & Interfaces 2021, 13 (7) , 9006-9014.
    9. Jianyang Zhao, Diana Diaz-Dussan, Meng Wu, Yi-Yang Peng, Jinquan Wang, Hongbo Zeng, Wei Duan, Lingxue Kong, Xiaojuan Hao, Ravin Narain. Dual-Cross-Linked Network Hydrogels with Multiresponsive, Self-Healing, and Shear Strengthening Properties. Biomacromolecules 2021, 22 (2) , 800-810.
    10. Kai Feng, Lun Peng, Liyin Yu, Yuqing Zheng, Rui Chen, Weidong Zhang, Gaojian Chen. Universal Antifogging and Antimicrobial Thin Coating Based on Dopamine-Containing Glycopolymers. ACS Applied Materials & Interfaces 2020, 12 (24) , 27632-27639.
    11. Gongyan Liu, Jun Xiang, Qiongfen Xia, Kaijun Li, Hui Yan, Ling Yu. Fabrication of Durably Antibacterial Cotton Fabrics by Robust and Uniform Immobilization of Silver Nanoparticles via Mussel-Inspired Polydopamine/Polyethyleneimine Coating. Industrial & Engineering Chemistry Research 2020, 59 (20) , 9666-9678.
    12. Kazunari Yoshida, Leona Takahashi, Akito Takashima, Yasuhiro Fujii, Izumi Nishio. Antireflection in Green Lacewing Wings with Random Height Surface Protrusions. Langmuir 2020, 36 (15) , 4207-4213.
    13. Haoshuang Wu, Li Yang, Rifang Luo, Li Li, Tiantian Zheng, Kaiyang Huang, Yumei Qin, Xia Yang, Xingdong Zhang, Yunbing Wang. A drug-free cardiovascular stent functionalized with tailored collagen supports in-situ healing of vascular tissues. Nature Communications 2024, 15 (1)
    14. Survanshu Saxena, Payel Sen, Leyla Soleymani, Todd Hoare. Anti‐Fouling Polymer or Peptide‐Modified Electrochemical Biosensors for Improved Biosensing in Complex Media. Advanced Sensor Research 2024, 121
    15. Mohit Saraf, Prateek, Rahul Ranjan, Bhuvaneshwari Balasubramaniam, Vijay Kumar Thakur, Raju Kumar Gupta. Polydopamine‐Enabled Biomimetic Surface Engineering of Materials: New Insights and Promising Applications. Advanced Materials Interfaces 2023, 38
    16. Cuihong Chen, Lin Chen, Congyang Mao, Liguo Jin, Shuilin Wu, Yufeng Zheng, Zhenduo Cui, Zhaoyang Li, Yu Zhang, Shengli Zhu, Hui Jiang, Xiangmei Liu. Natural Extracts for Antibacterial Applications. Small 2023, 36
    17. Lorand Bonda, Janita Müller, Lukas Fischer, Maryna Löwe, Alexej Kedrov, Stephan Schmidt, Laura Hartmann. Facile Synthesis of Catechol-Containing Polyacrylamide Copolymers: Synergistic Effects of Amine, Amide and Catechol Residues in Mussel-Inspired Adhesives. Polymers 2023, 15 (18) , 3663.
    18. Meng Wu, Jifang Liu, Xiaogang Wang, Hongbo Zeng. Recent advances in antimicrobial surfaces via tunable molecular interactions: Nanoarchitectonics and bioengineering applications. Current Opinion in Colloid & Interface Science 2023, 66 , 101707.
    19. Hossein Omidian, Renae L. Wilson, Niloofar Babanejad. Bioinspired Polymers: Transformative Applications in Biomedicine and Regenerative Medicine. Life 2023, 13 (8) , 1673.
    20. Fabian Kopsch, Astrid Drechsler, Martina Priebs, Anja Caspari, Anett Müller, Sarah Lentz, Jens Friedrichs, Alla Synytska. Zwitterionic Polymer Brushes and Core‐Shell Particles Based thereon for Control of Biofouling. Macromolecular Chemistry and Physics 2023, 224 (9)
    21. De Liu, Huming Wang, Xuxu Dong, Xiaodan Liu, Sergi Dosta, Chao Zhang, Pan Cao. Enhancing antibacterial and anticorrosion properties of 304 stainless steel surfaces: a multi-modification approach based on DA/PEI/SiO2/AMPs. Journal of Coatings Technology and Research 2023, 20 (3) , 979-994.
    22. Xionggang Chen, Jianhong Zhou, Yu Qian, LingZhou Zhao. Antibacterial coatings on orthopedic implants. Materials Today Bio 2023, 19 , 100586.
    23. Youbing Mu, Qian Sun, Bowen Li, Xiaobo Wan. Advances in the Synthesis and Applications of Mussel-Inspired Polymers. Polymer Reviews 2023, 63 (1) , 1-39.
    24. Ziqiang Liu, Yunfeng Wu, Fengxia Lan, Gaoyi Xie, Mengchen Zhang, Chunping Ma, Jianbo Jia. Improvement of permeability and antifouling performance of PVDF membranes via dopamine-assisted deposition of zwitterionic copolymer. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2023, 656 , 130505.
    25. Weiyu Dou, Feng Qi, Yangyang Li, Fangfang Wei, Qitao Hu, Zhikan Yao, Jing Wang, Lin Zhang, Zhe Tang. Charge-biased nanofibrous membranes with uniform charge distribution and hemocompatibility for enhanced selective adsorption of endotoxin from plasma. Journal of Membrane Science 2023, 666 , 121134.
    26. Tim Egghe, Rino Morent, Richard Hoogenboom, Nathalie De Geyter. Substrate-independent and widely applicable deposition of antibacterial coatings. Trends in Biotechnology 2023, 41 (1) , 63-76.
    27. Kazuhiko Ishihara. Biomimetic materials based on zwitterionic polymers toward human-friendly medical devices. Science and Technology of Advanced Materials 2022, 23 (1) , 498-524.
    28. Anika Benozir Asha, Artjima Ounkaew, Yi-Yang Peng, Mohammad Reza Gholipour, Kazuhiko Ishihara, Yang Liu, Ravin Narain. Bioinspired antifouling and antibacterial polymer coating with intrinsic self-healing property. Biomaterials Science 2022, 11 (1) , 128-139.
    29. Wan Peng, Birong Fan, Youxin Li, Yaning Dong, Weijian Qian, Xiaoxue Ji, Donglin Gan, Pingsheng Liu, Jian Shen. Layer-by-layer construction of zwitterionic/biguanide polymers on silicone rubber as an antifouling and bactericidal coating. Journal of Materials Chemistry B 2022, 10 (39) , 8013-8023.
    30. Qingchen Lu, Xiaoming Zhang, Ngie Hing Wong, Jaka Sunarso, Nana Li. Anti-biofouling polyvinylidene fluoride/quaternized polyvinyl alcohol ultrafiltration membrane selectively separates aromatic contaminants from wastewater by host–guest interactions. Separation and Purification Technology 2022, 296 , 121387.
    31. Yu Fu, Qingrong Wu, Wanying Yang, Shouxin Liu. Synthesis and Properties of Hydrogels on Medical Titanium Alloy Surface by Modified Dopamine Adhesion. Gels 2022, 8 (8) , 458.
    32. Kathleen Hennig, Wolfdietrich Meyer. Synthesis and Characterization of Catechol-Containing Polyacrylamides with Adhesive Properties. Molecules 2022, 27 (13) , 4027.
    33. Kazuhiko Ishihara, Kyoko Fukazawa. Cell-membrane-inspired polymers for constructing biointerfaces with efficient molecular recognition. Journal of Materials Chemistry B 2022, 10 (18) , 3397-3419.
    34. Santiago Arango-Santander. Bioinspired Topographic Surface Modification of Biomaterials. Materials 2022, 15 (7) , 2383.
    35. Xingran Zhang, Jinxing Ma, Junjian Zheng, Ruobin Dai, Xueye Wang, Zhiwei Wang. Recent advances in nature-inspired antifouling membranes for water purification. Chemical Engineering Journal 2022, 432 , 134425.
    36. Chaoqi Chen, Zhaoshuang Li, Xiangzhou Li, Chuntao Kuang, Xiubo Liu, Zhanqian Song, He Liu, Yu Shan. Dual-functional antimicrobial coating based on the combination of zwitterionic and quaternary ammonium cation from rosin acid. Composites Part B: Engineering 2022, 232 , 109623.
    37. Gabrijela Begić, Mirna Petković Didović, Sanja Lučić Blagojević, Ivana Jelovica Badovinac, Jure Žigon, Marko Perčić, Olga Cvijanović Peloza, Ivana Gobin. Adhesion of Oral Bacteria to Commercial d-PTFE Membranes: Polymer Microstructure Makes a Difference. International Journal of Molecular Sciences 2022, 23 (6) , 2983.
    38. Li Yang, Haoshuang Wu, Yuqi Liu, Qiongfen Xia, Yuan Yang, Nuoya Chen, Ming Yang, Rifang Luo, Gongyan Liu, Yunbing Wang. A robust mussel-inspired zwitterionic coating on biodegradable poly(L-lactide) stent with enhanced anticoagulant, anti-inflammatory, and anti-hyperplasia properties. Chemical Engineering Journal 2022, 427 , 130910.
    39. Ileana Ielo, Fausta Giacobello, Angela Castellano, Silvia Sfameni, Giulia Rando, Maria Rosaria Plutino. Development of Antibacterial and Antifouling Innovative and Eco-Sustainable Sol–Gel Based Materials: From Marine Areas Protection to Healthcare Applications. Gels 2022, 8 (1) , 26.
    40. Anika Benozir Asha, Yangjun Chen, Ravin Narain. Bioinspired dopamine and zwitterionic polymers for non-fouling surface engineering. Chemical Society Reviews 2021, 50 (20) , 11668-11683.
    41. Peng Dong, Xiulin Shu, Ruqun Peng, Shunying Lu, Xiaobao Xie, Qingshan Shi. Macroporous zwitterionic composite cryogel based on chitosan oligosaccharide for antifungal application. Materials Science and Engineering: C 2021, 128 , 112327.
    42. Anwesha Sarkar, Siavash Soltanahmadi, Jianshe Chen, Jason R. Stokes. Oral tribology: Providing insight into oral processing of food colloids. Food Hydrocolloids 2021, 117 , 106635.
    43. Wansuk Choi, Min Gyu Shin, Cheol Hun Yoo, Hosik Park, You-In Park, Jong Suk Lee, Jung-Hyun Lee. Desalination membranes with ultralow biofouling via synergistic chemical and topological strategies. Journal of Membrane Science 2021, 626 , 119212.
    44. Chao Zhou, Jun-Tao Zhou, Cheng-Ju Sheng, Dicky Pranantyo, Yan Pan, Xiao-Jia Huang. Robust and Self-healable Antibiofilm Multilayer Coatings. Chinese Journal of Polymer Science 2021, 39 (4) , 425-440.
    45. Jun Xiang, Ruixin Zhu, Shiying Lang, Hui Yan, Gongyan Liu, Biyu Peng. Mussel-inspired immobilization of zwitterionic silver nanoparticles toward antibacterial cotton gauze for promoting wound healing. Chemical Engineering Journal 2021, 409 , 128291.
    46. Angelika E. Neitzel, Guilhem X. De Hoe, Matthew V. Tirrell. Expanding the structural diversity of polyelectrolyte complexes and polyzwitterions. Current Opinion in Solid State and Materials Science 2021, 25 (2) , 100897.
    47. Kazuhiko Ishihara, Kohei Suzuki, Yuuki Inoue, Kyoko Fukazawa. Effects of molecular architecture of photoreactive phospholipid polymer on adsorption and reaction on substrate surface under aqueous condition. Journal of Biomaterials Science, Polymer Edition 2021, 32 (4) , 419-437.
    48. Trevor B. Donadt, Rong Yang. Amphiphilic Polymer Thin Films with Enhanced Resistance to Biofilm Formation at the Solid–Liquid–Air Interface. Advanced Materials Interfaces 2021, 8 (5)
    49. Anna Michalicha, Krzysztof Pałka, Agata Roguska, Marcin Pisarek, Anna Belcarz. Polydopamine-coated curdlan hydrogel as a potential carrier of free amino group-containing molecules. Carbohydrate Polymers 2021, 256 , 117524.
    50. Tahmineh Rahmani Eliato, Joshua T. Smith, Zhen Tian, Eun-Sik Kim, Wonseok Hwang, Cheryl P. Andam, Young Jo Kim. Melanin pigments extracted from horsehair as antibacterial agents. Journal of Materials Chemistry B 2021, 9 (6) , 1536-1545.
    51. Kazuhiko Ishihara, Yoichiro Kozaki, Yuuki Inoue, Kyoko Fukazawa. Biomimetic phospholipid polymers for suppressing adsorption of saliva proteins on dental hydroxyapatite substrate. Journal of Applied Polymer Science 2021, 138 (6)
    52. Chao Zhou, Juntao Zhou, Xiaoqing Ma, Dicky Pranantyo, Jingjing Li, Liqun Xu, Vinh X. Truong. Robust anti-infective multilayer coatings with rapid self-healing property. Materials Science and Engineering: C 2021, 121 , 111828.
    53. Indu Singh, Gagan Dhawan, Seema Gupta, Pradeep Kumar. Recent Advances in a Polydopamine-Mediated Antimicrobial Adhesion System. Frontiers in Microbiology 2021, 11
    54. Huiwen Pang, Shujun Zhao, Liuting Mo, Zhong Wang, Wei Zhang, Anmin Huang, Shifeng Zhang, Jianzhang Li. Mussel‐inspired bio‐based water‐resistant soy adhesives with low‐cost dopamine analogue‐modified silkworm silk Fiber. Journal of Applied Polymer Science 2020, 137 (23)
    55. Liman Zhao, Rongrong Chen, Liangjie Lou, Xiaoyan Jing, Qi Liu, Jingyuan Liu, Jing Yu, Peili Liu, Jun Wang. Layer-by-Layer-Assembled antifouling films with surface microtopography inspired by Laminaria japonica. Applied Surface Science 2020, 511 , 145564.
    56. Shigehito Osawa, Masato Kashiwakura, Hiro Yamaguchi, Hidenori Otsuka. Influence of anchoring segments on methacrylate-based comb-type hydrophilic polymer segments coated on silica surfaces using the grafting-to method. Colloid and Interface Science Communications 2020, 36 , 100258.
    57. Shengnan Zhou, Haixun Ji, Linjing Liu, Sijia Feng, Yuqin Fu, Yu Yang, Changli Lü. Mussel-inspired coordination functional polymer brushes-decorated rGO-stabilized silver nanoparticles composite for antibacterial application. Polymer Chemistry 2020, 11 (16) , 2822-2830.
    58. Emma A. Cuello, Lucinda E. Mulko, Cesar A. Barbero, Diego F. Acevedo, Edith I. Yslas. Development of micropatterning polyimide films for enhanced antifouling and antibacterial properties. Colloids and Surfaces B: Biointerfaces 2020, 188 , 110801.
    59. Fiona Baldwin, Tim J. Craig, Anna I. Shiel, Timothy Cox, Kyueui Lee, Jason P. Mansell. Polydopamine-Lysophosphatidate-Functionalised Titanium: A Novel Hybrid Surface Finish for Bone Regenerative Applications. Molecules 2020, 25 (7) , 1583.
    60. Bohan Cheng, Kazuhiko Ishihara, Hirotaka Ejima. Bio-inspired immobilization of low-fouling phospholipid polymers via a simple dipping process: a comparative study of phenol, catechol and gallol as tethering groups. Polymer Chemistry 2020, 11 (2) , 249-253.
    61. Yuanyuan He, Xinyuan Wan, Kecen Xiao, Weiwei Lin, Jiehua Li, Zhen Li, Feng Luo, Hong Tan, Jianshu Li, Qiang Fu. Anti-biofilm surfaces from mixed dopamine-modified polymer brushes: synergistic role of cationic and zwitterionic chains to resist staphyloccocus aureus. Biomaterials Science 2019, 7 (12) , 5369-5382.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Your Mendeley pairing has expired. Please reconnect