Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein
- Mingshu ZhangMingshu ZhangKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaMore by Mingshu Zhang
- Zhifei FuZhifei FuKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100149, ChinaMore by Zhifei Fu
- Changqing LiChangqing LiKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaMore by Changqing Li
- Anyuan LiuAnyuan LiuKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaSchool of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Anyuan Liu
- Dingming PengDingming PengKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100149, ChinaMore by Dingming Peng
- Fudong XueFudong XueKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100149, ChinaMore by Fudong Xue
- Wenting HeWenting HeKey Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaMore by Wenting He
- Shan GaoShan GaoCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100149, ChinaKey Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, ChinaMore by Shan Gao
- Fan XuFan XuKey Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, ChinaMore by Fan Xu
- Dan XuDan XuCollege of Biological Science and Engineering, Institute of Life Sciences, Fuzhou University, Fuzhou 350116, ChinaMore by Dan Xu
- Ling YuanLing YuanCenter for Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan 410078, ChinaMore by Ling Yuan
- Fa ZhangFa ZhangKey Lab of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, ChinaMore by Fa Zhang
- Zhiheng XuZhiheng XuCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100149, ChinaState Key Laboratory of Molecular Developmental Biology, CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaMore by Zhiheng Xu
- Tao Xu*Tao Xu*E-mail: [email protected]. Phone: +86-10-64888524.College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100149, ChinaNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing100101, ChinaMore by Tao Xu
- Pingyong Xu*Pingyong Xu*E-mail: [email protected]. Phone: +86-10-64888808. Fax: +86-10-64888808.Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100149, ChinaNational Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing100101, ChinaMore by Pingyong Xu
Abstract
Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1–0.25 s) with large field-of-view (76 × 9.4 μm2 −76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific “parallel three-pillar” structure in the neuronal-glial cell junction, helping to elucidate glial cell “locking” and support of neurons during Drosophila embryogenesis.