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CNC-Milled Superhydrophobic Macroporous Monoliths for 3D Cell Culture
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    CNC-Milled Superhydrophobic Macroporous Monoliths for 3D Cell Culture
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    ACS Applied Bio Materials

    Cite this: ACS Appl. Bio Mater. 2020, 3, 8, 4747–4750
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    https://doi.org/10.1021/acsabm.0c00719
    Published July 21, 2020
    Copyright © 2020 American Chemical Society

    Abstract

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    High-strength macroporous monoliths can be obtained by the simple mixing of boehmite nanofiber aqueous acetate dispersions with methyltrimethoxysilane. On the boehmite nanofiber–polymethylsilsesquioxane monoliths, we can fabricate structures smaller than a millimeter in size by computer numerical control (CNC) milling, resulting in a machined surface that is superhydrophobic and biocompatible. Using this strategy, we fabricated a superhydrophobic multiwell plate that holds water droplets to produce 3D cell culture environments for various cell types. We expect these superhydrophobic monoliths to have future applications in 3D tissue construction.

    Copyright © 2020 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsabm.0c00719.

    • Experimental procedures, photograph of a BNF–PMSQ monolith, properties of BNF–PMSQ monoliths, microstructure of BNF–PMSQ monoliths, mechanical properties of BNF–PMSQ monoliths, SEM images of a CNC-milled flat surface, superhydrophobicity, schematics of the microneedle array and multiwell plate, photographs of spheroids, confocal fluorescent images of spheroids, and changes in density and diameter of spheroids (PDF)

    • Microstructure of a BNF–PMSQ macroporous monolith (MP4)

    • Time-lapse movie during CNC milling (MP4)

    • Formation of a Janus water droplet on a water-repellent microneedle array fabricated by CNC milling (MP4)

    • Confocal fluorescent image sequence (MP4)

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    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

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    This article is cited by 11 publications.

    1. Gen Hayase. Boehmite Nanofiber–Melamine–Formaldehyde Composite Aerogels and Derivatives for Thermal Insulation and Optical Applications. ACS Applied Nano Materials 2023, 6 (15) , 13869-13873. https://doi.org/10.1021/acsanm.3c01980
    2. Kazuyo Ito, Yuta Iijima, Tomoki Misumi, Gen Hayase, Kazuki Tamura, Kenji Ikushima, Daisuke Yoshino. Biochemical state in tissue can be detected through ultrasound signal. 2024https://doi.org/10.1101/2024.12.27.630453
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    4. Hongyu Yin, Zhoujun Wang, Xiang Zhang, Weifeng Zhao, Ran Wei, Changsheng Zhao. Janus PES-based architectures integrated dense membrane with porous monolith for simultaneous plasma separation and toxins adsorption. Chemical Engineering Journal 2024, 502 , 157944. https://doi.org/10.1016/j.cej.2024.157944
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    7. Bohui Zhang, Wentao Duan, Yilin Wang, Lei Dai, Bolei Cai, Liang Kong, Jinhai Fan, Guangjian Zhang, Lei Wang, Weiwei Wu, Ruizhi Ning. Recent advances of cellulose nanofiber-based materials in cell culture: From population to single-cell. TrAC Trends in Analytical Chemistry 2023, 166 , 117159. https://doi.org/10.1016/j.trac.2023.117159
    8. Xiaoou Wei, Chao Liu, Xinai Zhang, Zhihua Li, Xinyu Wang, Yiwei Xu, Jiyong Shi, Quancai Sun, Michael N. Routledge, Di Zhang, Xiaobo Zou. An integrated 3D cell-based electrochemical biosensor for neurotoxicity assessment of organophosphorus pesticides. Sensors and Actuators B: Chemical 2023, 376 , 132941. https://doi.org/10.1016/j.snb.2022.132941
    9. Chunyu Li, Zehua Hou, Pan Li, Guomin Zhang, Liangjiu Bai, Wenxiang Wang, Hou Chen, Huawei Yang, Lixia Yang. Phytic acid-assist for self-healing nanocomposite hydrogels with surface functionalization of cellulose nanocrystals via SI-AGET ATRP. Cellulose 2023, 30 (2) , 1087-1102. https://doi.org/10.1007/s10570-022-04936-5
    10. Mizuki Tenjimbayashi, Kengo Manabe. A review on control of droplet motion based on wettability modulation: principles, design strategies, recent progress, and applications. Science and Technology of Advanced Materials 2022, 23 (1) , 473-497. https://doi.org/10.1080/14686996.2022.2116293
    11. Michele Ferrari, Francesca Cirisano, M. Carmen Morán. Super Liquid-repellent Surfaces and 3D Spheroids Growth. Frontiers in Bioscience-Landmark 2022, 27 (5) https://doi.org/10.31083/j.fbl2705144
    12. Sora Tomita, Terumasa Ito, Kazuyuki Iwaikawa, Gen Hayase, Daisuke Yoshino, Kazuhiko Misawa, , . Quantitative measurement of low-concentration analytes using Raman spectroscopy during droplet evaporation for therapeutic drug monitoring. 2021, 61. https://doi.org/10.1117/12.2614937

    ACS Applied Bio Materials

    Cite this: ACS Appl. Bio Mater. 2020, 3, 8, 4747–4750
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsabm.0c00719
    Published July 21, 2020
    Copyright © 2020 American Chemical Society

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