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Shape-Morphing Fibrous Hydrogel/Elastomer Bilayers Fabricated by a Combination of 3D Printing and Melt Electrowriting for Muscle Tissue Regeneration

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    Shape-Morphing Fibrous Hydrogel/Elastomer Bilayers Fabricated by a Combination of 3D Printing and Melt Electrowriting for Muscle Tissue Regeneration
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    • Juan Uribe-Gomez
      Juan Uribe-Gomez
      Faculty of Engineering Sciences, University of Bayreuth, Thoma Strasse 36A, Bayreuth 95447, Germany
      More by Juan Uribe-Gomez
    • Andrés Posada-Murcia
      Andrés Posada-Murcia
      Faculty of Engineering Sciences, University of Bayreuth, Thoma Strasse 36A, Bayreuth 95447, Germany
      More by Andrés Posada-Murcia
    • Amit Shukla
      Amit Shukla
      Faculty of Engineering Sciences, University of Bayreuth, Thoma Strasse 36A, Bayreuth 95447, Germany
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    • Mert Ergin
      Mert Ergin
      Faculty of Engineering Sciences, University of Bayreuth, Thoma Strasse 36A, Bayreuth 95447, Germany
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    • Gissela Constante
      Gissela Constante
      Faculty of Engineering Sciences, University of Bayreuth, Thoma Strasse 36A, Bayreuth 95447, Germany
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    • Indra Apsite
      Indra Apsite
      Faculty of Engineering Sciences, University of Bayreuth, Thoma Strasse 36A, Bayreuth 95447, Germany
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    • Dulle Martin
      Dulle Martin
      Forschungszentrum Jülich GmbH Jülich Centre for Neutron Science (JCNS-1) and Institute for Complex Systems (ICS-1), Wilhelm-Johnen-Straße, Jülich 52428, Germany
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    • Madeleine Schwarzer
      Madeleine Schwarzer
      Leibniz Institute of Polymer Research Dresden e. V., Hohe Straße 6, Dresden 01069, Germany
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      Orcidhttp://orcid.org/0000-0001-6204-1025
    • Anja Caspari
      Anja Caspari
      Leibniz Institute of Polymer Research Dresden e. V., Hohe Straße 6, Dresden 01069, Germany
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    • Alla Synytska
      Alla Synytska
      Leibniz Institute of Polymer Research Dresden e. V., Hohe Straße 6, Dresden 01069, Germany
      Faculty of Mathematics and Science, Institute of Physical Chemistry and Polymer Physics, Dresden University of Technology, Dresden 01062, Germany
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      Orcidhttp://orcid.org/0000-0002-0643-7524
    • Sahar Salehi
      Sahar Salehi
      Department of Biomaterials, University of Bayreuth, Prof.-Rüdiger-Bormann Strasse 1, 95447 Bayreuth, Germany
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    • Leonid Ionov*
      Leonid Ionov
      Faculty of Engineering Sciences  and  Bavarian Polymer Institute, University of Bayreuth, Thoma Strasse 36A, Bayreuth 95447, Germany
      *Email: [email protected]
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      Orcidhttp://orcid.org/0000-0002-0770-6140
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    ACS Applied Bio Materials

    Cite this: ACS Appl. Bio Mater. 2021, 4, 2, 1720–1730
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    https://doi.org/10.1021/acsabm.0c01495
    Published January 26, 2021
    Copyright © 2021 American Chemical Society
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    Abstract

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    This paper reports an approach for the fabrication of shape-changing bilayered scaffolds, which allow the growth of aligned skeletal muscle cells, using a combination of 3D printing of hyaluronic acid hydrogel, melt electrowriting of thermoplastic polycaprolactone-polyurethane elastomer, and shape transformation. The combination of the selected materials and fabrication methods allows a number of important advantages such as biocompatibility, biodegradability, and suitable mechanical properties (elasticity and softness of the fibers) similar to those of important components of extracellular matrix (ECM), which allow proper cell alignment and shape transformation. Myoblasts demonstrate excellent viability on the surface of the shape-changing bilayer, where they occupy space between fibers and align along them, allowing efficient cell patterning inside folded structures. The bilayer scaffold is able to undergo a controlled shape transformation and form multilayer scroll-like structures with cells encapsulated inside. Overall, the importance of this approach is the fabrication of tubular constructs with a patterned interior that can support the proliferation and alignment of muscle cells for muscle tissue regeneration.

    Copyright © 2021 American Chemical Society

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsabm.0c01495.

    • Infrared spectra of PCL-PU 121; NMR spectra of PCL-PU 121; thermogravimetric analysis of PCL-PU 121; NMR spectra of HA-MA; differential scanning calorimetry in time scale of PCL-diol 2000 kDa, PCL-PU 110, PCL-PU 121, and PCL-PU 143; C2C12 myoblast cell culture presented with detailed significant differences after quantification of cell viability after 1, 3, and 7 days of culture and cell metabolic activity after 1, 3, and 7 days of culture measured by Alamar Blue Assay (PDF)

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    This article is cited by 40 publications.

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    ACS Applied Bio Materials

    Cite this: ACS Appl. Bio Mater. 2021, 4, 2, 1720–1730
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsabm.0c01495
    Published January 26, 2021
    Copyright © 2021 American Chemical Society
    Request reuse permissions

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