Effect of Divalent Metal Cations on the Conformation, Elastic Behavior, and Controlled Release of a Photocrosslinked Protein Engineered HydrogelClick to copy article linkArticle link copied!
- Yao WangYao WangDepartment of Chemical and Biomolecular Engineering, New York University Tandon School of Engineering, Brooklyn, New York 11201, United StatesMore by Yao Wang
- Xiaole WangXiaole WangDepartment of Chemical and Biomolecular Engineering, New York University Tandon School of Engineering, Brooklyn, New York 11201, United StatesMore by Xiaole Wang
- Jin Kim Montclare*Jin Kim Montclare*Email: [email protected]Department of Chemical and Biomolecular Engineering, New York University Tandon School of Engineering, Brooklyn, New York 11201, United StatesDepartment of Chemistry, New York University, New York, New York 10003, United StatesDepartment of Biomaterials, New York University College of Dentistry, New York, New York 10010, United StatesDepartment of Radiology, New York University Langone Health, New York, New York 10016, United StatesMore by Jin Kim Montclare
Abstract
We investigate the effect of Zn2+, Cu2+, and Ni2+ coordination on the conformation, mechanical properties, contraction, and small-molecule drug encapsulation and release of a photocrosslinked protein-engineered hydrogel, CEC-D. The treatment of the CEC-D hydrogel with divalent metal (M2+) results in significant conformational changes where a loss in structure is observed with Zn2+, while both Cu2+ and Ni2+ induce a blueshift. The relationship of M2+ to mechanical properties illustrates a trend, while the CEC-D hydrogel in the presence of 2 mM Cu2+ reveals the highest increase in G′ to 14.4 ± 0.7 kPa followed by 9.7 ± 0.9 kPa by addition of 2 mM Zn2+, and a decrease to 1.1 ± 0.2 kPa is demonstrated in the presence of 2 mM Ni2+. A similar observation in M2+ responsiveness emerges where CEC-D hydrogels contract into a condensed state of 2.6-fold for Cu2+, 2.4-fold for Zn2+, and 1.6-fold for Ni2+. Furthermore, CEC-D hydrogels coordinated with M2+ demonstrate control over the encapsulation and release of the small molecule curcumin. The trend of release is opposite of the mechanical and contraction properties with a 70.0 ± 5.3% release with Ni2+, 64.2 ± 1.2% release with Zn2+, and 42.3 ± 11.3 release with Cu2+. Taken together, these results indicate that the CEC-D hydrogel tuned by M2+ is a promising drug delivery platform with tunable physicochemical properties.
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