Transferrin Immobilized Graphene Oxide Nanocomposite for Targeted Cancer Chemodynamic Therapy via Increasing Intracellular Labile Fe2+ ConcentrationClick to copy article linkArticle link copied!
- Ashish K ShuklaAshish K ShuklaBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, IndiaMore by Ashish K Shukla
- Mohini VermaMohini VermaBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, IndiaMore by Mohini Verma
- Manik BathlaManik BathlaBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, IndiaMore by Manik Bathla
- Shiwani RandhawaShiwani RandhawaBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, IndiaMore by Shiwani Randhawa
- Trilok Chand SainiTrilok Chand SainiBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, IndiaMore by Trilok Chand Saini
- Avnesh KumariAvnesh KumariBiotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, IndiaMore by Avnesh Kumari
- Amitabha Acharya*Amitabha Acharya*Email: [email protected], [email protected]; Tel. (Office): +91-1894-233339, Ext. 397; Fax: +91-1894-230433.Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, IndiaMore by Amitabha Acharya
Abstract
Recently, different alternative regulated cell death (RCD) pathways, viz., necroptosis, pyroptosis, ferroptosis, cuproptosis etc., have been explored as important targets for the development of cancer medications in recent years, as these can change the immunogenicity of the tumor microenvironment (TME) and will finally lead to the inhibition of cancer progression and metastasis. Here, we report the development of transferrin immobilized graphene oxide (Tfn@GOAPTES) nanocomposite as a therapeutic strategy toward cancer cell killing. The electrostatic immobilization of Tfn on the GOAPTES surface was confirmed by different spectroscopy and microscopy techniques. The Tfn immobilization was found to be ∼74 ± 4%, whereas the stability of the protein on the GO surface suggested a robust nature of the nanocomposite. The MTT assay suggested that Tfn@GOAPTES exhibited cytotoxicity toward HeLa cells via increased lipid peroxidation and DNA damage. Western blot studies resulted in decreased expression of acetylation on lysine 40 of α-tubulin and increased expression of LC3a/b for Tfn@GOAPTES treated HeLa cells, suggesting autophagy to be the main cause of the cell death mechanism. Overall, we predict that the present approach can be used as a therapeutic strategy for cancer cell killing via selective induction of a high concentration of intracellular iron.
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This article is cited by 2 publications.
- Mohini Verma, Shiwani Randhawa, Manik Bathla, Nandini Teji, Amitabha Acharya. Strategic use of nanomaterials as double-edged therapeutics to control carcinogenesis
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regulation of dysbiosis and bacterial infection: current status and future prospects. Journal of Materials Chemistry B 2025, 13
(16)
, 4770-4790. https://doi.org/10.1039/D4TB02409E
- Nem Singh, Dahee Kim, Sunhong Min, Eunji Kim, Shiyoung Kim, Yu Shrike Zhang, Heemin Kang, Jong Seung Kim. Multimodal synergistic ferroptosis cancer therapy. Coordination Chemistry Reviews 2025, 522 , 216236. https://doi.org/10.1016/j.ccr.2024.216236
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