Detection of the Enzymatic Cleavage of DNA through Supramolecular Chiral Induction to a Cationic PolythiopheneClick to copy article linkArticle link copied!
- Mathieu FossépréMathieu FossépréLaboratory for Chemistry of Novel Materials, Centre of Innovation and Research in Materials and Polymers (CIRMAP), University of Mons (UMONS), 20 Place du Parc, Mons B-7000, BelgiumMore by Mathieu Fossépré
- Marie E. TrévisanMarie E. TrévisanLaboratory for Chemistry of Novel Materials, Centre of Innovation and Research in Materials and Polymers (CIRMAP), University of Mons (UMONS), 20 Place du Parc, Mons B-7000, BelgiumMore by Marie E. Trévisan
- Valentine CyriaqueValentine CyriaqueProteomics and Microbiology Lab, Research Institute for Biosciences, University of Mons (UMONS), Avenue du Champs de Mars 6, Mons 7000, BelgiumMore by Valentine Cyriaque
- Ruddy WattiezRuddy WattiezProteomics and Microbiology Lab, Research Institute for Biosciences, University of Mons (UMONS), Avenue du Champs de Mars 6, Mons 7000, BelgiumMore by Ruddy Wattiez
- David BeljonneDavid BeljonneLaboratory for Chemistry of Novel Materials, Centre of Innovation and Research in Materials and Polymers (CIRMAP), University of Mons (UMONS), 20 Place du Parc, Mons B-7000, BelgiumMore by David Beljonne
- Sébastien RicheterSébastien RicheterInstitut Charles Gerhardt ICGM, UMR 5253 CNRS-ENSCM-UM, Université de Montpellier, CC1701 Place Eugène Bataillon, Montpellier Cedex 05F-34095, FranceMore by Sébastien Richeter
- Sébastien ClémentSébastien ClémentInstitut Charles Gerhardt ICGM, UMR 5253 CNRS-ENSCM-UM, Université de Montpellier, CC1701 Place Eugène Bataillon, Montpellier Cedex 05F-34095, FranceMore by Sébastien Clément
- Mathieu Surin*Mathieu Surin*Email: [email protected]Laboratory for Chemistry of Novel Materials, Centre of Innovation and Research in Materials and Polymers (CIRMAP), University of Mons (UMONS), 20 Place du Parc, Mons B-7000, BelgiumMore by Mathieu Surin
Abstract
Water-soluble π-conjugated polymers are increasingly envisioned in biosensors, in which their unique optical and electronic properties permit a highly sensitive detection of biomolecular targets. In particular, cationic π-conjugated polymers are attractive for DNA sensing technologies, through the use of the fluorescence signals either in physiological solutions or in thin films. However, in the context of enzymatic activity assays, fluorescence-based methods require covalently labeling DNA with a dye or an antibody and are limited to short time scale due to dye photobleaching. In this frame, we report here a novel possible approach to probe the cleavage of DNA by a restriction enzyme, in continuous and without covalently labeled DNA substrate. This is achieved by exploiting unique chiroptical signals arising from the chiral induction of DNA to a poly[3-(6′-(trimethylphosphonium)hexyl)thiophene-2,5-diyl] upon interaction. The cleavage of DNA by HpaI, an endonuclease enzyme, is monitored through circular dichroism (CD) signals in the spectral range where the polymer absorbs light, i.e., far away from the spectral ranges of both DNA and the enzyme. We compare the results to a conventional noncontinuous assay by polyacrylamide gel electrophoresis, and we demonstrate that induced CD signals are effective in probing the enzymatic activity. By means of molecular dynamics simulations and calculations of CD spectra, we bring molecular insights into the structure of DNA/polymer supramolecular complexes before and after the cleavage of DNA. We show that the cleavage of DNA modifies the dynamics and the organization of the polymer backbone induced by the DNA helix. Altogether, our results provide detailed spectroscopic and structural insights into the enzymatic cleavage of DNA in interaction with a π-conjugated polymer, which could be helpful for developing chiroptical detection tools to monitor the catalytic activity in real time.
Cited By
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by ACS Publications if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
This article is cited by 11 publications.
- Lisa Peterhans, Eliana Nicolaidou, Polydefkis Diamantis, Elisa Alloa, Mario Leclerc, Mathieu Surin, Sébastien Clément, Ursula Rothlisberger, Natalie Banerji, Sophia C. Hayes. Structural and Photophysical Templating of Conjugated Polyelectrolytes with Single-Stranded DNA. Chemistry of Materials 2020, 32
(17)
, 7347-7362. https://doi.org/10.1021/acs.chemmater.0c02251
- Regina C. So, Analyn C. Carreon-Asok. Molecular Design, Synthetic Strategies, and Applications of Cationic Polythiophenes. Chemical Reviews 2019, 119
(21)
, 11442-11509. https://doi.org/10.1021/acs.chemrev.8b00773
- Binghuan Huang, Xinlin Liu, Dongming Xing. Recent advances in water-soluble polythiophenes for biomedical applications. European Polymer Journal 2024, 213 , 113096. https://doi.org/10.1016/j.eurpolymj.2024.113096
- Debora Quadretti, Martina Marinelli, Elisabetta Salatelli, Filippo Pierini, Alberto Zanelli, Massimiliano Lanzi. Effects of Water/Alcohol Soluble Cationic Polythiophenes as Cathode Interlayers for Eco‐Friendly Solar Cells. Macromolecular Chemistry and Physics 2023, 224
(6)
https://doi.org/10.1002/macp.202200422
- Markéta Paloncýová, Martin Pykal, Petra Kührová, Pavel Banáš, Jiří Šponer, Michal Otyepka. Computer Aided Development of Nucleic Acid Applications in Nanotechnologies. Small 2022, 18
(49)
https://doi.org/10.1002/smll.202204408
- Martina Marinelli, Massimiliano Lanzi, Filippo Pierini, Yasamin Ziai, Alberto Zanelli, Debora Quadretti, Francesca Di Maria, Elisabetta Salatelli. Ionic Push–Pull Polythiophenes: A Further Step towards Eco-Friendly BHJ Organic Solar Cells. Polymers 2022, 14
(19)
, 3965. https://doi.org/10.3390/polym14193965
- Clément Kotras, Maxime Leclercq, Maxime Roger, Camille Bouillon, Antonio Recupido, Aurélien Lebrun, Yannick Bessin, Philippe Gerbier, Sébastien Richeter, Sébastien Ulrich, Sébastien Clément, Mathieu Surin. Fluorescent Dynamic Covalent Polymers for DNA Complexation and Templated Assembly. Molecules 2022, 27
(19)
, 6648. https://doi.org/10.3390/molecules27196648
- Mathieu Fossépré, Inbal Tuvi‐Arad, David Beljonne, Sébastien Richeter, Sébastien Clément, Mathieu Surin. Binding Mode Multiplicity and Multiscale Chirality in the Supramolecular Assembly of DNA and a π‐Conjugated Polymer. ChemPhysChem 2020, 21
(23)
, 2543-2552. https://doi.org/10.1002/cphc.202000630
- Chunzhi Cui, Dong Hyuk Park, Dong June Ahn. Organic Semiconductor–DNA Hybrid Assemblies. Advanced Materials 2020, 32
(51)
https://doi.org/10.1002/adma.202002213
- Xiaoxiao Cheng, Tengfei Miao, Yilin Qian, Zhengbiao Zhang, Wei Zhang, Xiulin Zhu. Supramolecular Chirality in Azobenzene-Containing Polymer System: Traditional Postpolymerization Self-Assembly Versus In Situ Supramolecular Self-Assembly Strategy. International Journal of Molecular Sciences 2020, 21
(17)
, 6186. https://doi.org/10.3390/ijms21176186
- Mathieu Surin, Sébastien Ulrich. From Interaction to Function in DNA‐Templated Supramolecular Self‐Assemblies. ChemistryOpen 2020, 9
(4)
, 480-498. https://doi.org/10.1002/open.202000013
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.