Enhanced Uptake and Endosomal Release of LbL Microcarriers Functionalized with Reversible Fusion ProteinsClick to copy article linkArticle link copied!
- Kira SchefflerKira SchefflerInstitute for Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig, D-04107 Leipzig, GermanyMore by Kira Scheffler
- Nicole C. BilzNicole C. BilzInstitute of Virology, Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyMore by Nicole C. Bilz
- Mandy BruecknerMandy BruecknerInstitute for Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig, D-04107 Leipzig, GermanyMore by Mandy Brueckner
- Megan L. StaniferMegan L. StaniferSchaller Research Group at CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University Hospital, 69120 Heidelberg, GermanyMore by Megan L. Stanifer
- Steeve BoulantSteeve BoulantSchaller Research Group at CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University Hospital, 69120 Heidelberg, GermanyResearch Group “Cellular Polarity and Viral Infection” (F140), German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanyMore by Steeve Boulant
- Claudia ClausClaudia ClausInstitute of Virology, Faculty of Medicine, University of Leipzig, 04103 Leipzig, GermanyMore by Claudia Claus
- Uta Reibetanz*Uta Reibetanz*E-mail: [email protected]Institute for Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig, D-04107 Leipzig, GermanyMore by Uta Reibetanz
Abstract
The efficient application of smart drug-delivery systems requires further improvement of their cellular uptake and in particular their release from endolysosomal compartments into the cytoplasm of target cells. The usage of virus proteins allows for such developments, as viruses have evolved efficient entry mechanisms into the cell, mediated by their fusion proteins. In our investigations, the transferability of the glycoprotein G which is a fusion protein of the vesicular stomatitis virus (VSV-G) onto the surface of a layer-by-layer (LbL) designed microcarrier was investigated. The assembly of VSV-G as a reversible viral fusion protein onto LbL microcarriers indeed induced an enhanced uptake rate on Vero cells as well as a fast and efficient release of the intact carriers from endolysosomes into the cytoplasm. Additionally, neither virus-associated effects on cellular viability nor activation of an interferon response were detected. Our study emphasizes the suitability of VSV-G as an efficient surface functionalization of drug-delivery systems.
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