Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing
- Jakub SopoušekJakub SopoušekDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 753/5 625 00, Brno, Czech RepublicCentral European Institute of Technology CEITEC, Masaryk University, Kamenice 753/5 625 00, Brno, Czech RepublicMore by Jakub Sopoušek
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- Jakub VěžníkJakub VěžníkCentral European Institute of Technology CEITEC, Masaryk University, Kamenice 753/5 625 00, Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5 625 00, Brno, Czech RepublicMore by Jakub Věžník
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- Petr SkládalPetr SkládalDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 753/5 625 00, Brno, Czech RepublicCentral European Institute of Technology CEITEC, Masaryk University, Kamenice 753/5 625 00, Brno, Czech RepublicMore by Petr Skládal
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- Karel Lacina*Karel Lacina*E-mail: [email protected] (K.L.).Central European Institute of Technology CEITEC, Masaryk University, Kamenice 753/5 625 00, Brno, Czech RepublicMore by Karel Lacina
Abstract
Blockage of a nanopore by an analyte molecule has emerged as a promising concept for electrochemical biosensing. Nanoporous structures can be formed on the electrode surface simply by packing spherical nanoparticles in a dense planar arrangement. Modification of the nanoparticles with human serum albumin (HSA) and its interaction with the corresponding antibody (anti-HSA) can induce nanopore-blockage which significantly hinders permeation of the redox probe ([Fe(CN6)]4–/3–). Interfaces of different parameters were studied using Electrochemical Impedance Spectroscopy (EIS), and counterintuitively, the influence of charge of the nanoparticles and other immobilized entities played a substantial role in the measurement. Our study reveals dominant effects including the presence of mixed output signal and resolves corresponding EIS biosensing-related challenges. Consequently, blocking the nanopores was introduced as an efficient technique which enables the application of EIS-based biosensing to real-world analytical issues.
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