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Paper Microfluidics and Tailored Gold Nanoparticles for Nonenzymatic, Colorimetric Multiplex Biomarker Detection

  • Tomás Pinheiro
    Tomás Pinheiro
    CENIMAT|i3N, Departamento de Ciência de Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa and CEMOP/UNINOVA, Campus da Caparica, 2829-516 Caparica, Portugal
  • Ana C. Marques
    Ana C. Marques
    CENIMAT|i3N, Departamento de Ciência de Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa and CEMOP/UNINOVA, Campus da Caparica, 2829-516 Caparica, Portugal
  • Patrícia Carvalho
    Patrícia Carvalho
    SINTEF Materials and Chemistry, PB 124, Blindern, NO-0314 Oslo, Norway
  • Rodrigo Martins
    Rodrigo Martins
    CENIMAT|i3N, Departamento de Ciência de Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa and CEMOP/UNINOVA, Campus da Caparica, 2829-516 Caparica, Portugal
  • , and 
  • Elvira Fortunato*
    Elvira Fortunato
    CENIMAT|i3N, Departamento de Ciência de Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa and CEMOP/UNINOVA, Campus da Caparica, 2829-516 Caparica, Portugal
    *Email: [email protected]
Cite this: ACS Appl. Mater. Interfaces 2021, 13, 3, 3576–3590
Publication Date (Web):January 15, 2021
https://doi.org/10.1021/acsami.0c19089
Copyright © 2021 American Chemical Society

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    Abstract

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    The plasmonic properties of gold nanoparticles (AuNPs) are a promising tool to develop sensing alternatives to traditional, enzyme-catalyzed reactions. The need for sensing alternatives, especially in underdeveloped areas of the world, has given rise to the application of nonenzymatic sensing approaches paired with cellulosic substrates to biochemical analysis. Herein, we present three individual, low-step, wet-chemistry, colorimetric assays for three target biomarkers, namely, glucose, uric acid, and free cholesterol, relevant in diabetes control and their translation into paper-based assays and microfluidic platforms for multiplexed analysis. For glucose determination, an in situ AuNPs synthesis approach was applied into the developed μPAD, giving semiquantitative measures in the physiologically relevant range. For uric acid and cholesterol determination, modified AuNPs were used to functionalize paper with a gold-on-paper approach with the optical properties changing based on different aggregation degrees and hydrophobic properties of particles dependent on analyte concentration. These paper-based assays show sensitivity ranges and limits of detection compatible for target analyte level determination and detection limits comparable to those of similar enzymatic, colorimetric systems, relying only on plasmonic transduction without the need for enzymatic activity or other chromogenic substrates. The resulting paper-based assays were integrated into a single 3D, multiplex paper-based device using paper microfluidics, showing the capability for performing different colorimetric assays with distinct requirements in terms of sample flow and sample uptake in test zones using a combination of both horizontal and vertical flows inside the same device. The presented device allows for multiparametric, colorimetric measures of different metabolite levels from a single complex sample matrix drop using digital color analysis, showing the potential for development of low-cost, low-complexity tools for diagnostics toward the point-of-care.

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsami.0c19089.

    • Supplemental experimental procedures for glucose-capped, 2-TU-modified, and digitonin-conjugated AuNPs synthesis, schemes, and figures (PDF)

    • 3D multiplex μPAD operation testing video (MP4)

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    19. Feifei Ma, Zhe Zhao, Jiahui Huang, Qing Xiong, Shaohua Xu, Zhenyu Lin. Hybridization chain reaction assisted multicolor immunosensor for sensitively detection of human chorionic gonadotropin. Talanta 2024, 270 , 125578. https://doi.org/10.1016/j.talanta.2023.125578
    20. Xue Jin, Jia Liu, Jiaxi Wang, Mingxia Gao, Xiangmin Zhang. Paper-based uric acid assay in whole blood samples by Zn2+ protein precipitation and enzyme-free colorimetric detection. Analytical and Bioanalytical Chemistry 2024, 416 (7) , 1589-1597. https://doi.org/10.1007/s00216-024-05160-9
    21. Yuan Zhou, Aiping Cui, Dongliu Xiang, Yanan Luan, Qing Wang, Jin Huang, Jianbo Liu, Xiaohai Yang, Kemin Wang. Point-of-care testing of four chronic disease biomarkers in blood based on a low cost and low system complexity microfluidic chip with integrated oxygen-sensitive membrane. Sensors and Actuators B: Chemical 2024, 398 , 134734. https://doi.org/10.1016/j.snb.2023.134734
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    23. Jianping Guan, Meng Wang, Yu Xiong, Qi Liu, Xiaoqing Chen. A luminescent MOF-based nonenzymatic probe for colorimetric/photothermal/fluorescence triple-mode assay of uric acid in body fluids. Talanta 2024, 267 , 125201. https://doi.org/10.1016/j.talanta.2023.125201
    24. Zhicheng Jin, Wonjun Yim, Maurice Retout, Emily Housel, Wenbin Zhong, Jiajing Zhou, Michael S. Strano, Jesse V. Jokerst. Colorimetric sensing for translational applications: from colorants to mechanisms. Chemical Society Reviews 2024, 173 https://doi.org/10.1039/D4CS00328D
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    31. Saikrushna Jena, Divya Gaur, Nidhi C. Dubey, Bijay P. Tripathi. Advances in paper based isothermal nucleic acid amplification tests for water-related infectious diseases. International Journal of Biological Macromolecules 2023, 242 , 125089. https://doi.org/10.1016/j.ijbiomac.2023.125089
    32. Pornchanok Punnoy, Tatiya Siripongpreda, Trairak Pisitkun, Nadnudda Rodthongkum, Pranut Potiyaraj. Alternative platform for COVID-19 diagnosis based on AuNP-modified lab-on-paper. The Analyst 2023, 148 (12) , 2767-2775. https://doi.org/10.1039/D3AN00595J
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    41. Yangjiayi Xiang, Cong Hu, Gou Wu, Shilin Xu, Yan Li. Nanomaterial-based microfluidic systems for cancer biomarker detection: Recent applications and future perspectives. TrAC Trends in Analytical Chemistry 2023, 158 , 116835. https://doi.org/10.1016/j.trac.2022.116835
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    43. Xiaodong Sun, Yongxin Liu, Bing Niu, Qin Chen, Xueen Fang, . Rapid identification and quantitation of single plant seed allergen using paper-based microfluidics. PLOS ONE 2022, 17 (12) , e0266775. https://doi.org/10.1371/journal.pone.0266775
    44. Hamed Tavakoli, Samayeh Mohammadi, Xiaochun Li, Guanglei Fu, XiuJun Li. Microfluidic platforms integrated with nano-sensors for point-of-care bioanalysis. TrAC Trends in Analytical Chemistry 2022, 157 , 116806. https://doi.org/10.1016/j.trac.2022.116806
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    46. Yue Hou, Cong-Cong Lv, Yan-Li Guo, Xiao-Hu Ma, Wei Liu, Yan Jin, Bao-Xin Li, Min Yang, Shi-Yin Yao. Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing. Journal of Analysis and Testing 2022, 6 (3) , 247-273. https://doi.org/10.1007/s41664-021-00204-w
    47. Jing Yang, Lin Huang, Kun Qian. Nanomaterials‐assisted metabolic analysis toward in vitro diagnostics. Exploration 2022, 2 (3) https://doi.org/10.1002/EXP.20210222
    48. Shrishty Bakshi, Aaron J. Snoswell, Kei Yeung Kwok, Lung Hei Cheng, Tariq A. Altalhi, Abel Santos, Amirali Popat, Tushar Kumeria. Spray‐n‐Sense: Sprayable Nanofibers for On‐Site Chemical Sensing. Advanced Functional Materials 2022, 32 (16) https://doi.org/10.1002/adfm.202103496
    49. Muzahidul I. Anik, Niaz Mahmud, Abdullah Al Masud, Maruf Hasan. Gold nanoparticles (GNPs) in biomedical and clinical applications: A review. Nano Select 2022, 3 (4) , 792-828. https://doi.org/10.1002/nano.202100255
    50. Yanping Wang, Yanfeng Gao, Yi Yin, Yongchun Pan, Yuzhen Wang, Yujun Song. Nanomaterial-assisted microfluidics for multiplex assays. Microchimica Acta 2022, 189 (4) https://doi.org/10.1007/s00604-022-05226-4
    51. Madhusudhan Alle, Rajkumar Bandi, Garima Sharma, Ramakrishna Dadigala, Seung-Hwan Lee, Jin-Chul Kim. Gold nanoparticles spontaneously grown on cellulose nanofibrils as a reusable nanozyme for colorimetric detection of cholesterol in human serum. International Journal of Biological Macromolecules 2022, 201 , 686-697. https://doi.org/10.1016/j.ijbiomac.2022.01.158
    52. Congran Jin, Ziqian Wu, John H. Molinski, Junhu Zhou, Yundong Ren, John X.J. Zhang. Plasmonic nanosensors for point-of-care biomarker detection. Materials Today Bio 2022, 14 , 100263. https://doi.org/10.1016/j.mtbio.2022.100263
    53. Yanling Zhao, Yanfei Wen, Xing Hu, Bing Zhang, . A Colorimetric Immunoassay Based on g-C3N4@Fe3O4 Nanocomposite for Detection of Carcinoembryonic Antigen. Journal of Analytical Methods in Chemistry 2022, 2022 , 1-7. https://doi.org/10.1155/2022/6966470
    54. Kaitlynn R. Mitchell, Joule E. Esene, Adam T. Woolley. Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices. Analytical and Bioanalytical Chemistry 2022, 414 (1) , 167-180. https://doi.org/10.1007/s00216-021-03553-8
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    56. Jingji Liu, Boyang Zhang, Yajun Zhang, Yiqiang Fan. Fluid control with hydrophobic pillars in paper-based microfluidics. Journal of Micromechanics and Microengineering 2021, 31 (12) , 127002. https://doi.org/10.1088/1361-6439/ac35c9
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