Cerebrospinal Fluid Leak Detection with a Carbon Nanotube-Based Field-Effect Transistor Biosensing PlatformClick to copy article linkArticle link copied!
- Wenting ShaoWenting ShaoDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United StatesMore by Wenting Shao
- Galina V. ShurinGalina V. ShurinDepartment of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15260, United StatesMore by Galina V. Shurin
- Xiaoyun HeXiaoyun HeDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United StatesMore by Xiaoyun He
- Zidao ZengZidao ZengDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United StatesMore by Zidao Zeng
- Michael R. ShurinMichael R. ShurinDepartment of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15260, United StatesMore by Michael R. Shurin
- Alexander Star*Alexander Star*Email: [email protected]Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United StatesDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United StatesMore by Alexander Star
Abstract

Cerebrospinal fluid (CSF) leakage may lead to life-threatening complications if not detected promptly. However, gel electrophoresis, the gold-standard test for confirming CSF leakage by detecting beta2-transferrin (β2-Tf), requires 3–6 h and is labor-intensive. We developed a new β2-Tf detection platform for rapid identification of CSF leakage. The three-step design, which includes two steps of affinity chromatography and a rapid sensing step using a semiconductor-enriched single-walled carbon nanotube field-effect transistor (FET) sensor, circumvented the lack of selectivity that antitransferrin antibody exhibits for transferrin isoforms and markedly shortened the detection time. Furthermore, three different sensing configurations for the FET sensor were investigated for obtaining the optimal β2-Tf sensing results. Finally, body fluid (CSF and serum) tests employing our three-step strategy demonstrated high sensitivity, suggesting its potential to be used as a rapid diagnostic tool for CSF leakage.
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