Nanoparticle–Protein Interaction: Demystifying the Correlation between Protein Corona and Aggregation Phenomena
- Larissa Fernanda FerreiraLarissa Fernanda FerreiraBrazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970 Campinas, BrazilPrograma de Pós-Graduação em Biotecnociências, Universidade Federal do ABC, 09210-580 Santo André, BrazilMore by Larissa Fernanda Ferreira
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- Agustín Silvio PiccoAgustín Silvio PiccoInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Fac. de Cs. Exactas, Universidad Nacional de La Plata─CONICET, Boulevard 113 y 64, 1900 La Plata, ArgentinaMore by Agustín Silvio Picco
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- Flávia Elisa GaldinoFlávia Elisa GaldinoBrazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970 Campinas, BrazilInstitute of Chemistry (IQ), University of Campinas (UNICAMP), P.O. Box 6154, 13083-970 Campinas, BrazilMore by Flávia Elisa Galdino
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- Lindomar Jose Calumby AlbuquerqueLindomar Jose Calumby AlbuquerqueBrazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970 Campinas, Brazil
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- Jean-François Berret*Jean-François Berret*J.-F.B.: email, [email protected]; phone, +33 1 57 27 61 47.Université Paris Cité, CNRS, Matière et Systèmes Complexes, 75013 Paris, FranceMore by Jean-François Berret
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- Mateus Borba Cardoso*Mateus Borba Cardoso*M.B.C.: email, [email protected]; phone, +55 19 3512 10.Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970 Campinas, BrazilPrograma de Pós-Graduação em Biotecnociências, Universidade Federal do ABC, 09210-580 Santo André, BrazilInstitute of Chemistry (IQ), University of Campinas (UNICAMP), P.O. Box 6154, 13083-970 Campinas, BrazilMore by Mateus Borba Cardoso
Abstract
Protein corona formation and nanoparticles’ aggregation have been heavily discussed over the past years since the lack of fine-mapping of these two combined effects has hindered the targeted delivery evolution and the personalized nanomedicine development. We present a multitechnique approach that combines dynamic light and small-angle X-ray scattering techniques with cryotransmission electron microscopy in a given fashion that efficiently distinguishes protein corona from aggregates formation. This methodology was tested using ∼25 nm model silica nanoparticles incubated with either model proteins or biologically relevant proteomes (such as fetal bovine serum and human plasma) in low and high ionic strength buffers to precisely tune particle-to-protein interactions. In this work, we were able to differentiate protein corona, small aggregates formation, and massive aggregation, as well as obtain fractal information on the aggregates reliably and straightforwardly. The strategy presented here can be expanded to other particle-to-protein mixtures and might be employed as a quality control platform for samples that undergo biological tests.
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