Structure–Property Relationship Based on the Amino Acid Composition of Recombinant Spider Silk Proteins for Potential Biomedical Applications
- Sarah LentzSarah LentzLehrstuhl Biomaterialien, Fakultät für Ingenieurwissenschaften, Universität Bayreuth, Rüdiger-Bormann-Str. 1, 95447 Bayreuth, GermanyMore by Sarah Lentz
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- Vanessa T. TrossmannVanessa T. TrossmannLehrstuhl Biomaterialien, Fakultät für Ingenieurwissenschaften, Universität Bayreuth, Rüdiger-Bormann-Str. 1, 95447 Bayreuth, GermanyMore by Vanessa T. Trossmann
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- Christian B. BorknerChristian B. BorknerLehrstuhl Biomaterialien, Fakultät für Ingenieurwissenschaften, Universität Bayreuth, Rüdiger-Bormann-Str. 1, 95447 Bayreuth, GermanyMore by Christian B. Borkner
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- Vivien BeyersdorferVivien BeyersdorferLehrstuhl Biomaterialien, Fakultät für Ingenieurwissenschaften, Universität Bayreuth, Rüdiger-Bormann-Str. 1, 95447 Bayreuth, GermanyMore by Vivien Beyersdorfer
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- Markus RottmarMarkus RottmarLaboratory for Materials-Biology Interactions, Empa Swiss Federal Laboratories for Materials Science and Technology, CH-9014 St. Gallen, SwitzerlandMore by Markus Rottmar
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- Thomas Scheibel*Thomas Scheibel*Email: [email protected]Lehrstuhl Biomaterialien, Fakultät für Ingenieurwissenschaften, Universität Bayreuth, Rüdiger-Bormann-Str. 1, 95447 Bayreuth, GermanyBayerisches Polymerinstitut (BPI), Bayreuther Zentrum für Kolloide und Grenzflächen (BZKG), Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), Bayreuther Materialzentrum (BayMAT), Universität Bayreuth, Universitätsstr. 30, 95440 Bayreuth, GermanyMore by Thomas Scheibel
Abstract
Improving biomaterials by engineering application-specific and adjustable properties is of increasing interest. Most of the commonly available materials fulfill the mechanical and physical requirements of relevant biomedical applications, but they lack biological functionality, including biocompatibility and prevention of microbial infestation. Thus, research has focused on customizable, application-specific, and modifiable surface coatings to cope with the limitations of existing biomaterials. In the case of adjustable degradation and configurable interaction with body fluids and cells, these coatings enlarge the applicability of the underlying biomaterials. Silks are interesting coating materials, e.g., for implants, since they exhibit excellent biocompatibility and mechanical properties. Herein, we present putative implant coatings made of five engineered recombinant spider silk proteins derived from the European garden spider Araneus diadematus fibroins (ADF), differing in amino acid sequence and charge. We analyzed the influence of the underlying amino acid composition on wetting behavior, blood compatibility, biodegradability, serum protein adsorption, and cell adhesion. The outcome of the comparison indicates that spider silk coatings can be engineered for explicit biomedical applications.
Cited By
This article is cited by 3 publications.
- Sarah Lentz, Vanessa Tanja Trossmann, Thomas Scheibel. Selective Topography Directed Cell Adhesion on Spider Silk Surfaces. Advanced Materials Interfaces 2023, 10
(5)
, 2201936. https://doi.org/10.1002/admi.202201936
- Vanessa Tanja Trossmann, Thomas Scheibel. Design of Recombinant Spider Silk Proteins for Cell Type Specific Binding. Advanced Healthcare Materials 2023, 117 , 2202660. https://doi.org/10.1002/adhm.202202660
- Alaitz Etxabide, Maite Arregi, Sara Cabezudo, Pedro Guerrero, Koro de la Caba. Whey Protein Films for Sustainable Food Packaging: Effect of Incorporated Ascorbic Acid and Environmental Assessment. Polymers 2023, 15
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, 387. https://doi.org/10.3390/polym15020387