ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Figure 1Loading Img
RETURN TO ISSUEPREVSurfaces, Interfaces...Surfaces, Interfaces, and ApplicationsNEXT

Microfluidic Droplet Stabilization via SPAAC Promoted Antibody Conjugation at the Water/Oil Interface

  • Robin Dufossez
    Robin Dufossez
    Bio-Functional Chemistry (UMR 7199), LabEx Medalis, University of Strasbourg, 74 Route du Rhin, 67400 Illkirch-Graffenstaden, France
  • Marie-Pierre Krafft
    Marie-Pierre Krafft
    Institut Charles Sadron (CNRS), University of Strasbourg, 67034 Strasbourg, France
  • Sylvain Ursuegui
    Sylvain Ursuegui
    MicroOmix, 74 Route du Rhin, 67400 Illkirch-Graffenstaden, France
  • Michel Mosser
    Michel Mosser
    Bio-Functional Chemistry (UMR 7199), LabEx Medalis, University of Strasbourg, 74 Route du Rhin, 67400 Illkirch-Graffenstaden, France
  • Safae Mouftakhir
    Safae Mouftakhir
    Bio-Functional Chemistry (UMR 7199), LabEx Medalis, University of Strasbourg, 74 Route du Rhin, 67400 Illkirch-Graffenstaden, France
    CNRS, Architecture et Réactivité de l’ARN (UPR 9002), University of Strasbourg, 67000 Strasbourg, France
  • Ketty Pernod
    Ketty Pernod
    MicroOmix, 74 Route du Rhin, 67400 Illkirch-Graffenstaden, France
    More by Ketty Pernod
  • Guilhem Chaubet
    Guilhem Chaubet
    Bio-Functional Chemistry (UMR 7199), LabEx Medalis, University of Strasbourg, 74 Route du Rhin, 67400 Illkirch-Graffenstaden, France
  • Michael Ryckelynck
    Michael Ryckelynck
    CNRS, Architecture et Réactivité de l’ARN (UPR 9002), University of Strasbourg, 67000 Strasbourg, France
  • , and 
  • Alain Wagner*
    Alain Wagner
    Bio-Functional Chemistry (UMR 7199), LabEx Medalis, University of Strasbourg, 74 Route du Rhin, 67400 Illkirch-Graffenstaden, France
    *Email: [email protected]
    More by Alain Wagner
Cite this: ACS Appl. Mater. Interfaces 2023, 15, 38, 45498–45505
Publication Date (Web):September 13, 2023
https://doi.org/10.1021/acsami.3c10655
Copyright © 2023 American Chemical Society

    Article Views

    479

    Altmetric

    -

    Citations

    -
    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (1)»

    Abstract

    Abstract Image

    Droplet-based microfluidics is leading the development of miniaturized, rapid, and sensitive version of enzyme-linked immunosorbent assays (ELISAs), a central method for protein detection. These assays involve the use of a functionalized surface able to selectively capture the desired analyte. Using the droplet’s oil water interface as a capture surface requires designing custom-perfluorinated fluorosurfactants bearing azide-containing polar groups, which spontaneously react when forming the droplet with strain-alkyne-functionalized antibodies solubilized in the aqueous phase. In this article, we present our research on the influence of the structure of surfactant’s hydrophilic heads on the efficiency of SPAAC functionalization and on the effect of this antibody grafting process on droplet stability. We have shown that while short linkers lead to high grafting efficiency, long linkers lead to high stability, and that an intermediate size is required to balance both parameters. In the described family of surfactants, the optimal structure proved to be a PEG4 linker connecting a polar di-azide head and a per-fluoropolyether tail (Krytox). We also found that grafting an increasing amount of antibody, thus increasing interface coverage, increases droplet stability. It thus appears that such a bi-partite system with a reactive fluoro-surfactant in the oil phase and reactive antibody counterpart in the aqueous phase gives access in situ to novel surfactant construct providing unexplored interface structures and droplet functionality.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsami.3c10655.

    • Additional experimental details such as materials, methods, synthesis route and protocols, molecules characterization (NMR, FT-IR, and HRMS), bioconjugation procedures, microfluidic experiments setup and procedures, tensiometry measurements setup and procedures, and confocal microscopy imaging and additional data (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article has not yet been cited by other publications.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect