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Hyperspectral Mapping of Human Primary and Stem Cells at Cell–Matrix Interfaces

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    Biological and Medical Applications of Materials and Interfaces

    Hyperspectral Mapping of Human Primary and Stem Cells at Cell–Matrix Interfaces
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    ACS Applied Materials & Interfaces

    Cite this: ACS Appl. Mater. Interfaces 2024, 16, 2, 2154–2165
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    https://doi.org/10.1021/acsami.3c17113
    Published January 5, 2024
    Copyright © 2024 American Chemical Society
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    Abstract

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    Extracellular matrices interface with cells to promote cell growth and tissue development. Given this critical role, matrix mimetics are introduced to enable biomedical materials ranging from tissue engineering scaffolds and tumor models to organoids for drug screening and implant surface coatings. Traditional microscopy methods are used to evaluate such materials in their ability to support exploitable cell responses, which are expressed in changes in cell proliferation rates and morphology. However, the physical imaging methods do not capture the chemistry of cells at cell–matrix interfaces. Herein, we report hyperspectral imaging to map the chemistry of human primary and embryonic stem cells grown on matrix materials, both native and artificial. We provide the statistical analysis of changes in lipid and protein content of the cells obtained from infrared spectral maps to conclude matrix morphologies as a major determinant of biochemical cell responses. The study demonstrates an effective methodology for evaluating bespoke matrix materials directly at cell–matrix interfaces.

    Copyright © 2024 American Chemical Society

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsami.3c17113.

    • Additional spectral maps, FTIR spectra and PCA plots of cells at cell–matrix interfaces; electron and atomic force micrographs of individual cells highlighting cell morphology and matrix fibers; and fluorescence micrographs of hESCs grown under various differentiating and nondifferentiating conditions (PDF)

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    Cited By

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    This article is cited by 1 publications.

    1. Mathias Charconnet, Javier Plou. Seeing beyond Labels: Optical Technologies Reshaping Cell Culture Monitoring in Biomedicine. ACS Applied Engineering Materials 2024, 2 (12) , 2790-2814. https://doi.org/10.1021/acsaenm.4c00613
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    ACS Applied Materials & Interfaces

    Cite this: ACS Appl. Mater. Interfaces 2024, 16, 2, 2154–2165
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsami.3c17113
    Published January 5, 2024
    Copyright © 2024 American Chemical Society
    Request reuse permissions

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