ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Figure 1Loading Img
RETURN TO ISSUEPREVResearch ArticleNEXT

Core–Shell Molecularly Imprinted Polymer Nanoparticles as Synthetic Antibodies in a Sandwich Fluoroimmunoassay for Trypsin Determination in Human Serum

View Author Information
Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France
*E-mail: [email protected] (B. Tse Sum Bui).
*E-mail: [email protected] (K. Haupt).
Cite this: ACS Appl. Mater. Interfaces 2017, 9, 29, 24476–24483
Publication Date (Web):July 5, 2017
https://doi.org/10.1021/acsami.7b05844
Copyright © 2017 American Chemical Society

    Article Views

    2059

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (1)»

    Abstract

    Abstract Image

    We describe the application of a fluorescently labeled water-soluble core–shell molecularly imprinted polymer (MIP) for fluorescence immunoassay (FIA) to detect trypsin. p-Aminobenzamidine (PAB), a competitive inhibitor of trypsin, was immobilized in the wells of a microtiter plate enabling the capture of trypsin in an oriented position, thus maintaining its native conformation. Fluorescent MIP nanoparticles, which bound selectively to trypsin, were used for quantification. The MIP was prepared by a multistep solid-phase synthesis approach on glass beads functionalized with PAB, orientating all trypsin molecules in the same way. The core–MIP was first synthesized, using a thermoresponsive polymer based on N-isopropylacrylamide, so as to enable its facile liberation from the immobilized template by a simple temperature change. The shell, mainly composed of allylamine to introduce primary amino groups for postconjugation of fluorescein isothiocyanate (FITC), was grafted in situ on the core–MIP, whose binding cavities were still bound and protected by the immobilized trypsin. The resulting core–shell MIP was endowed with a homogeneous population of high-affinity binding sites, all having the same orientation. The MIP has no or little cross-reactivity with other serine proteases and unrelated proteins. Our MIP-based FIA system was successfully applied to detect low trypsin concentrations spiked into nondiluted human serum with a low limit of quantification of 50 pM, which indicates the significant potential of this assay for analytical and biomedical diagnosis applications.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsami.7b05844.

    • Details of the synthesis of the iniferter, diethylthiocarbamoylsulfanyl acetic acid, the characterization of core–shell polymers (size, zeta potential, molecular weight, and fluorescence analysis), activation and characterization of microplates, optimization of cytochrome c amount for use in the quantification of trypsin, and quantification of trypsin by using cytochrome c as substrate (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 65 publications.

    1. Ana T. Silva, Rui Figueiredo, Manuel Azenha, Pedro A.S. Jorge, Carlos M. Pereira, José A. Ribeiro. Imprinted Hydrogel Nanoparticles for Protein Biosensing: A Review. ACS Sensors 2023, 8 (8) , 2898-2920. https://doi.org/10.1021/acssensors.3c01010
    2. Shengnan Shao, Shuang Gao, Yuan Li, Yongqin Lv. Rapid Screening and Synthesis of Abiotic Synthetic Receptors for Selective Bacterial Recognition. ACS Applied Materials & Interfaces 2023, 15 (13) , 16408-16419. https://doi.org/10.1021/acsami.2c22438
    3. Karsten Haupt, Paulina X. Medina Rangel, Bernadette Tse Sum Bui. Molecularly Imprinted Polymers: Antibody Mimics for Bioimaging and Therapy. Chemical Reviews 2020, 120 (17) , 9554-9582. https://doi.org/10.1021/acs.chemrev.0c00428
    4. Alejandra Mier, Sofia Nestora, Paulina X. Medina Rangel, Yannick Rossez, Karsten Haupt, Bernadette Tse Sum Bui. Cytocompatibility of Molecularly Imprinted Polymers for Deodorants: Evaluation on Human Keratinocytes and Axillary-Hosted Bacteria. ACS Applied Bio Materials 2019, 2 (8) , 3439-3447. https://doi.org/10.1021/acsabm.9b00388
    5. Rongrong Xing, Yanrong Wen, Yueru Dong, Yijia Wang, Qi Zhang, Zhen Liu. Dual Molecularly Imprinted Polymer-Based Plasmonic Immunosandwich Assay for the Specific and Sensitive Detection of Protein Biomarkers. Analytical Chemistry 2019, 91 (15) , 9993-10000. https://doi.org/10.1021/acs.analchem.9b01826
    6. Jingjing Xu, Franck Merlier, Bérangère Avalle, Vincent Vieillard, Patrice Debré, Karsten Haupt, Bernadette Tse Sum Bui. Molecularly Imprinted Polymer Nanoparticles as Potential Synthetic Antibodies for Immunoprotection against HIV. ACS Applied Materials & Interfaces 2019, 11 (10) , 9824-9831. https://doi.org/10.1021/acsami.8b22732
    7. Jingyi Luan, Ting Xu, John Cashin, Jeremiah J. Morrissey, Evan D. Kharasch, Srikanth Singamaneni. Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody. Analytical Chemistry 2018, 90 (13) , 7880-7887. https://doi.org/10.1021/acs.analchem.7b05470
    8. Cecília A. Mourão, Frank Bokeloh, Jingjing Xu, Elise Prost, Luminita Duma, Franck Merlier, Sônia M. A. Bueno, Karsten Haupt, and Bernadette Tse Sum Bui . Dual-Oriented Solid-Phase Molecular Imprinting: Toward Selective Artificial Receptors for Recognition of Nucleotides in Water. Macromolecules 2017, 50 (19) , 7484-7490. https://doi.org/10.1021/acs.macromol.7b01782
    9. Fan Ding, Yue Ma, Wensi Fan, Jingjing Xu, Guoqing Pan. Tailor-made molecular imprints for biological event intervention. Trends in Biotechnology 2024, 1 https://doi.org/10.1016/j.tibtech.2024.02.015
    10. Cem Esen, Francesco Canfarotta. Molecularly imprinted polymer nanoparticles: solid-phase synthesis and molecularly imprinted nanoparticle assay. 2024, 93-106. https://doi.org/10.1016/B978-0-443-15359-4.00004-8
    11. Guoning Chen, Shuxian Zhang, Xueqin Ma, Gidion Wilson, Rong Zong, Qiang Fu. Antibody mimics for precise identification of proteins based on molecularly imprinted polymers: Developments and prospects. Chemical Engineering Journal 2024, 480 , 148115. https://doi.org/10.1016/j.cej.2023.148115
    12. Fabio Di Nardo, Laura Anfossi, Claudio Baggiani. MIP-based immunoassays: A critical review. Analytica Chimica Acta 2023, 1277 , 341547. https://doi.org/10.1016/j.aca.2023.341547
    13. Wei Hu, Shasha Feng, Fubin Pei, Bin Du, Bing Liu, Xihui Mu, Zhaoyang Tong. A novel smartphone-integrated binary-emission molecularly imprinted fluorescence sensor embedded with MIL-101(Cr) for sensitive and real-time detection of protein. Talanta 2023, 260 , 124563. https://doi.org/10.1016/j.talanta.2023.124563
    14. Dominik Korol, Anna Kisiel, Maciej Cieplak, Agata Michalska, Piyush Sindhu Sharma, Krzysztof Maksymiuk. Synthesis of conducting molecularly imprinted polymer nanoparticles for estriol chemosensing. Sensors and Actuators B: Chemical 2023, 382 , 133476. https://doi.org/10.1016/j.snb.2023.133476
    15. Bernadette Tse Sum Bui, Alejandra Mier, Karsten Haupt. Molecularly Imprinted Polymers as Synthetic Antibodies for Protein Recognition: The Next Generation. Small 2023, 19 (13) https://doi.org/10.1002/smll.202206453
    16. Burcu Okutucu. Biomedicine Application of Nano-Molecularly Imprinted Polymers (NanoMIPs). Biomedical Materials & Devices 2023, 1 (1) , 419-425. https://doi.org/10.1007/s44174-022-00005-4
    17. Ruoyang Liu, Chi-Chiu Ko. Molecularly Imprinted Polymer-Based Luminescent Chemosensors. Biosensors 2023, 13 (2) , 295. https://doi.org/10.3390/bios13020295
    18. Xiaorong Zhang, Aysu Yarman, Mahdien Bagheri, Ibrahim M. El-Sherbiny, Rabeay Y. A. Hassan, Sevinc Kurbanoglu, Armel Franklin Tadjoung Waffo, Ingo Zebger, Tutku Ceren Karabulut, Frank F. Bier, Peter Lieberzeit, Frieder W. Scheller. Imprinted Polymers on the Route to Plastibodies for Biomacromolecules (MIPs), Viruses (VIPs), and Cells (CIPs). 2023https://doi.org/10.1007/10_2023_234
    19. Adriana Feldner, Julia Völkle, Felix Thier, Peter Lieberzeit. Biological, Bio-Derived, and Biomimetic Receptors in Mass-Sensitive Sensing. 2023, 143-224. https://doi.org/10.1007/5346_2023_30
    20. Chandra K. Dixit, Snehasis Bhakta, Kamil K. Reza, Ajeet Kaushik. Exploring molecularly imprinted polymers as artificial antibodies for efficient diagnostics and commercialization: A critical overview. Hybrid Advances 2022, 1 , 100001. https://doi.org/10.1016/j.hybadv.2022.100001
    21. Yali Chen, Zhen Zhang, Yujie Chen, Shufang Zhou, Qiliang Deng, Shuo Wang. Enhancement of inhibition rate of antibiotic against bacteria by molecularly imprinted nanoparticles targeting alarmone nucleotides as antibiotic adjuvants. Journal of Materials Chemistry B 2022, 10 (45) , 9438-9445. https://doi.org/10.1039/D2TB00641C
    22. Qi Zhou, Tsuyoshi Minami. An Oxytocin Sensor Based on an Organic Field-Effect Transistor Functionalized with a Molecularly Imprinted Polymer. 2022, 123-126. https://doi.org/10.1109/ICSJ55786.2022.10034697
    23. Matheus Siqueira Silva, Ana P. M. Tavares, Henrique Dipe de Faria, Maria Goreti Ferreira Sales, Eduardo Costa Figueiredo. Molecularly Imprinted Solid Phase Extraction Aiding the Analysis of Disease Biomarkers. Critical Reviews in Analytical Chemistry 2022, 52 (5) , 933-948. https://doi.org/10.1080/10408347.2020.1843131
    24. Dongwei Feng, Mingxing Ren, Yunfei Miao, Zerong Liao, Tuanjie Zhang, Shi Chen, Kaida Ye, Pengjie Zhang, Xiaolan Ma, Jiati Ni, Xueqiang Hu, Huanjun Li, Jirun Peng, Aiqin Luo, Lina Geng, Yulin Deng. Dual selective sensor for exosomes in serum using magnetic imprinted polymer isolation sandwiched with aptamer/graphene oxide based FRET fluorescent ignition. Biosensors and Bioelectronics 2022, 207 , 114112. https://doi.org/10.1016/j.bios.2022.114112
    25. Bernadette Tse Sum Bui, Karsten Haupt. Molecularly Imprinted Polymer Hydrogel Nanoparticles: Synthetic Antibodies for Cancer Diagnosis and Therapy. ChemBioChem 2022, 23 (8) https://doi.org/10.1002/cbic.202100598
    26. Yanting He, Zian Lin. Recent advances in protein-imprinted polymers: synthesis, applications and challenges. Journal of Materials Chemistry B 2022, 33 https://doi.org/10.1039/D2TB00273F
    27. Nébéwia Griffete, Laurent Michot, Carlo Gonzato. An easy synthesis of small, stable and water-compatible superparamagnetic protein-specific molecularly imprinted nanoparticles. Polymer 2022, 239 , 124446. https://doi.org/10.1016/j.polymer.2021.124446
    28. Doo Young Choi, Jin Chul Yang, Jinyoung Park. Optimization and characterization of electrochemical protein Imprinting on hemispherical porous gold patterns for the detection of trypsin. Sensors and Actuators B: Chemical 2022, 350 , 130855. https://doi.org/10.1016/j.snb.2021.130855
    29. M.A. Goicolea, A. Gómez-Caballero, M. Saumell-Esnaola, G. García del Caño, N. Unceta, J. Sallés, R.J. Barrio. A linear-polymer-based lactoferrin-selective recognition element for an ELISA mimic: A proof of concept. Analytica Chimica Acta 2022, 1191 , 339309. https://doi.org/10.1016/j.aca.2021.339309
    30. Alberto Gómez-Caballero, Ainhoa Elejaga-Jimeno, Gontzal García del Caño, Nora Unceta, Antonio Guerreiro, Miquel Saumell-Esnaola, Joan Sallés, M. Aránzazu Goicolea, Ramón J. Barrio. Solid-phase synthesis of imprinted nanoparticles as artificial antibodies against the C-terminus of the cannabinoid CB1 receptor: exploring a viable alternative for bioanalysis. Microchimica Acta 2021, 188 (11) https://doi.org/10.1007/s00604-021-05029-z
    31. Shan Chen, Jinli Fu, Shu Zhou, Xiaodan Wu, Sisi Tang, Pengfei Zhao, Zhaohui Zhang. An eco-friendly near infrared fluorescence molecularly imprinted sensor based on zeolite imidazolate framework-8 for rapid determination of trace trypsin. Microchemical Journal 2021, 168 , 106449. https://doi.org/10.1016/j.microc.2021.106449
    32. Nadja Leibl, Karsten Haupt, Carlo Gonzato, Luminita Duma. Molecularly Imprinted Polymers for Chemical Sensing: A Tutorial Review. Chemosensors 2021, 9 (6) , 123. https://doi.org/10.3390/chemosensors9060123
    33. Bing Zhang, Xing Hu, Yejing Jia, Jing Li, Zhihuan Zhao. Polyaniline@Au organic-inorganic nanohybrids with thermometer readout for photothermal immunoassay of tumor marker. Microchimica Acta 2021, 188 (3) https://doi.org/10.1007/s00604-021-04719-y
    34. Aysu Yarman, Sevinc Kurbanoglu, Ingo Zebger, Frieder W. Scheller. Simple and robust: The claims of protein sensing by molecularly imprinted polymers. Sensors and Actuators B: Chemical 2021, 330 , 129369. https://doi.org/10.1016/j.snb.2020.129369
    35. Jin Chul Yang, Suck Won Hong, Jinyoung Park. Improving Surface Imprinting Effect by Reducing Nonspecific Adsorption on Non-Imprinted Polymer Films for 2,4-D Herbicide Sensors. Chemosensors 2021, 9 (3) , 43. https://doi.org/10.3390/chemosensors9030043
    36. Alberto Gómez-Caballero, Nora Unceta, M. Aránzazu Goicolea, Ramón J. Barrio. Plastic Receptors Developed by Imprinting Technology as Smart Polymers Imitating Natural Behavior. 2021, 69-116. https://doi.org/10.1007/978-3-030-50457-1_5
    37. Recep Üzek, Esma Sari, Arben Merkoçi. Applications of Molecularly Imprinted Polymers/Fluorescence-Based (Nano) Sensors. 2021, 283-307. https://doi.org/10.1016/B978-0-12-822117-4.00011-3
    38. Hui He, Lingli Zhou, Zhen Liu. Advances in Protein Biomarker Assay via the Combination of Molecular Imprinting and Surface-enhanced Raman Scattering. Acta Chimica Sinica 2021, 79 (1) , 45. https://doi.org/10.6023/A20080364
    39. Mingfei Pan, Liping Hong, Xiaoqian Xie, Kaixin Liu, Jingying Yang, Shuo Wang. Nanomaterials‐Based Surface Protein Imprinted Polymers: Synthesis and Medical Applications. Macromolecular Chemistry and Physics 2021, 222 (1) https://doi.org/10.1002/macp.202000222
    40. Joseph W. Lowdon, Hanne Diliën, Pankaj Singla, Marloes Peeters, Thomas J. Cleij, Bart van Grinsven, Kasper Eersels. MIPs for commercial application in low-cost sensors and assays – An overview of the current status quo. Sensors and Actuators B: Chemical 2020, 325 , 128973. https://doi.org/10.1016/j.snb.2020.128973
    41. Ivan G.N. Silva, Danilo Mustafa. Luminescence enhancement by water replacement in Eu@COK-16 metal organic framework. Journal of Luminescence 2020, 227 , 117549. https://doi.org/10.1016/j.jlumin.2020.117549
    42. Annesha Mazumder, Syed Azeemuddin, Tapan K. Sau, Prabhakar Bhimalapuram. Role of Shape of Gold Nanoparticles in Sensing Biomolecules using Radio-Frequency based Sensors. 2020, 1-4. https://doi.org/10.1109/SENSORS47125.2020.9278747
    43. Annesha Mazumder, Syed Azeemuddin, Tapan K. Sau, Prabhakar Bhimalapuram. Study of Gold Particles in HFSS with Varying Physical Parameters and Arrangements. 2020, 529-532. https://doi.org/10.1109/NEMS50311.2020.9265619
    44. Jingjing Xu, Haohan Miao, Jixiang Wang, Guoqing Pan. Molecularly Imprinted Synthetic Antibodies: From Chemical Design to Biomedical Applications. Small 2020, 16 (27) https://doi.org/10.1002/smll.201906644
    45. Alvaro Garcia-Cruz, Todd Cowen, Annelies Voorhaar, Elena Piletska, Sergey A. Piletsky. Molecularly imprinted nanoparticles-based assay (MINA) – detection of leukotrienes and insulin. The Analyst 2020, 145 (12) , 4224-4232. https://doi.org/10.1039/D0AN00419G
    46. Yin Cui, Ming Li, Xia Hong, Daolin Du, Yue Ma. Solid-phase interfacial synthesis of dual-imprinted colloid particles for multifunctional nanomedicine development. Colloid and Interface Science Communications 2020, 36 , 100267. https://doi.org/10.1016/j.colcom.2020.100267
    47. Yue Zhang, Si Li, Xiao-Tong Ma, Xi-Wen He, Wen-You Li, Yu-Kui Zhang. Carbon dots-embedded epitope imprinted polymer for targeted fluorescence imaging of cervical cancer via recognition of epidermal growth factor receptor. Microchimica Acta 2020, 187 (4) https://doi.org/10.1007/s00604-020-4198-7
    48. Tianqi Zhang, Wen Zhang, Liang Liu, Yun Chen. Simultaneous detection of site-specific histone methylations and acetylation assisted by single template oriented molecularly imprinted polymers. The Analyst 2020, 145 (4) , 1376-1383. https://doi.org/10.1039/C9AN02360G
    49. Huiqi Zhang. Molecularly Imprinted Nanoparticles for Biomedical Applications. Advanced Materials 2020, 32 (3) https://doi.org/10.1002/adma.201806328
    50. Lingli Zhou, Yijia Wang, Rongrong Xing, Jin Chen, Jia Liu, Wei Li, Zhen Liu. Orthogonal dual molecularly imprinted polymer-based plasmonic immunosandwich assay: A double characteristic recognition strategy for specific detection of glycoproteins. Biosensors and Bioelectronics 2019, 145 , 111729. https://doi.org/10.1016/j.bios.2019.111729
    51. Rijun Gui, Hui Jin. Recent advances in synthetic methods and applications of photo-luminescent molecularly imprinted polymers. Journal of Photochemistry and Photobiology C: Photochemistry Reviews 2019, 41 , 100315. https://doi.org/10.1016/j.jphotochemrev.2019.08.002
    52. Paulina X. Medina Rangel, Sylvain Laclef, Jingjing Xu, Maria Panagiotopoulou, José Kovensky, Bernadette Tse Sum Bui, Karsten Haupt. Solid-phase synthesis of molecularly imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans. Scientific Reports 2019, 9 (1) https://doi.org/10.1038/s41598-019-40348-5
    53. Chenghong Sun, Linli Pan, Lei Zhang, Jiaojiao Huang, Dandan Yao, Chong-Zhi Wang, Yu Zhang, Nan Jiang, Lina Chen, Chun-su Yuan. A biomimetic fluorescent nanosensor based on imprinted polymers modified with carbon dots for sensitive detection of alpha-fetoprotein in clinical samples. The Analyst 2019, 144 (22) , 6760-6772. https://doi.org/10.1039/C9AN01065C
    54. Muhammad Imran Malik, Huma Shaikh, Ghulam Mustafa, Muhammad Iqbal Bhanger. Recent Applications of Molecularly Imprinted Polymers in Analytical Chemistry. Separation & Purification Reviews 2019, 48 (3) , 179-219. https://doi.org/10.1080/15422119.2018.1457541
    55. Wei Zhao, Bing Li, Sheng Xu, Xuewen Huang, Jing Luo, Ye Zhu, Xiaoya Liu. Electrochemical protein recognition based on macromolecular self-assembly of molecularly imprinted polymer: a new strategy to mimic antibody for label-free biosensing. Journal of Materials Chemistry B 2019, 7 (14) , 2311-2319. https://doi.org/10.1039/C9TB00220K
    56. Liuzheng Zheng, Xing Dong, Junjie Chi, Mi Sun, Chao Zhao, Hong Liu. Integration of patterned photonic nitrocellulose and microfluidic chip for fluorescent point-of-care testing of multiple targets. New Journal of Chemistry 2019, 43 (12) , 4808-4814. https://doi.org/10.1039/C9NJ00125E
    57. Hongxia Li, Mingming Yang, Deshuai Kong, Rui Jin, Xu Zhao, Fangmeng Liu, Xu Yan, Yuehe Lin, Geyu Lu. Sensitive fluorescence sensor for point-of-care detection of trypsin using glutathione-stabilized gold nanoclusters. Sensors and Actuators B: Chemical 2019, 282 , 366-372. https://doi.org/10.1016/j.snb.2018.11.077
    58. Takuya Kubo, Koji Otsuka. Molecularly Imprinted Materials. 2019, 159-178. https://doi.org/10.1002/9781119422587.ch5
    59. Liping Song, Lei Zhang, Kai Xu, Youju Huang, Pan Gao, Haiyan Fang, Jiawei Zhang, Zhihong Nie, Tao Chen. Fluorescent microsphere probe for rapid qualitative and quantitative detection of trypsin activity. Nanoscale Advances 2019, 1 (1) , 162-167. https://doi.org/10.1039/C8NA00111A
    60. Denise Riedel, Boris Mizaikoff. Surface Imprinted Micro- and Nanoparticles. 2019, 153-191. https://doi.org/10.1016/bs.coac.2019.06.001
    61. Zhifeng Xu, Peihong Deng, Junhua Li, Siping Tang, Ying Cui. Modification of mesoporous silica with molecular imprinting technology: A facile strategy for achieving rapid and specific adsorption. Materials Science and Engineering: C 2019, 94 , 684-693. https://doi.org/10.1016/j.msec.2018.10.032
    62. Qisi Liu, Kaina Zhang, Yuhao Jin, Xiangfeng Wang, Yuan Liu, Hailing Liu, Mengxia Xie. Phosphate-imprinted magnetic nanoparticles using phenylphosphonic acid as a template for excellent recognition of tyrosine phosphopeptides. Talanta 2018, 186 , 346-353. https://doi.org/10.1016/j.talanta.2018.04.025
    63. Leena Mattsson, Jingjing Xu, Claudia Preininger, Bernadette Tse Sum Bui, Karsten Haupt. Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix. Talanta 2018, 181 , 190-196. https://doi.org/10.1016/j.talanta.2018.01.010
    64. Lucia Cenci, Chiara Piotto, Paolo Bettotti, Alessandra Maria Bossi. Study on molecularly imprinted nanoparticle modified microplates for pseudo-ELISA assays. Talanta 2018, 178 , 772-779. https://doi.org/10.1016/j.talanta.2017.10.018
    65. Xiao-Yu Sun, Run-Tian Ma, Juan Chen, Yan-Ping Shi. Synthesis of magnetic molecularly imprinted nanoparticles with multiple recognition sites for the simultaneous and selective capture of two glycoproteins. Journal of Materials Chemistry B 2018, 6 (4) , 688-696. https://doi.org/10.1039/C7TB03001K

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect