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Biosensor for Point-of-Care Analysis of Immunoglobulins in Urine by Metal Enhanced Fluorescence from Gold Nanoparticles

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    Research Article

    Biosensor for Point-of-Care Analysis of Immunoglobulins in Urine by Metal Enhanced Fluorescence from Gold Nanoparticles
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    • Bartolomeo Della Ventura
      Bartolomeo Della Ventura
      Dipartimento di Fisica Ettore Pancini, Università di Napoli Federico II, Via Cintia, 26 Ed. 6, 80126 Napoli, Italy
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    • Monica Gelzo
      Monica Gelzo
      Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via Pansini 5, 80131 Napoli, Italy
      CEINGE-Biotecnologie avanzate s.c. a r.l., Via Gaetano Salvatore 486, 80145 Napoli, Italy
      More by Monica Gelzo
    • Edmondo Battista*
      Edmondo Battista
      Centro di Ricerca Interdipartimentale sui Biomateriali, Università degli Studi di Napoli “Federico II”, Ple Tecchio 80, 80125 Napoli, Italy
      *(E.B.) E-mail: [email protected]
      More by Edmondo Battista
      Orcidhttp://orcid.org/0000-0003-0915-9176
    • Alessandro Alabastri
      Alessandro Alabastri
      Electrical and Computer Engineering Department, Rice University, 6100 Main Street, Houston, Texas 77005, United States
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      Orcidhttp://orcid.org/0000-0001-6180-8052
    • Andrea Schirato
      Andrea Schirato
      Dipartimento di Fisica, Politecnico di Milano, Piazza L. da Vinci 32, 20133 Milano, Italy
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    • Giuseppe Castaldo
      Giuseppe Castaldo
      Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via Pansini 5, 80131 Napoli, Italy
      CEINGE-Biotecnologie avanzate s.c. a r.l., Via Gaetano Salvatore 486, 80145 Napoli, Italy
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    • Gaetano Corso
      Gaetano Corso
      Dipartimento di Medicina Clinica e Sperimentale, Università di Foggia, 70122 Viale Pinto, Foggia, Italy
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    • Francesco Gentile*
      Francesco Gentile
      Dipartimento di Ingegneria Elettrica e Tecnologie dell’Informazione, Università di Napoli Federico II, via Claudio 21, 80125 Napoli, Italy
      *(F.G.) E-mail: [email protected]
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      Orcidhttp://orcid.org/0000-0002-1724-6301
    • Raffaele Velotta*
      Raffaele Velotta
      Dipartimento di Fisica Ettore Pancini, Università di Napoli Federico II, Via Cintia, 26 Ed. 6, 80126 Napoli, Italy
      *(R.V.) E-mail: [email protected]
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      Orcidhttp://orcid.org/0000-0003-1077-8353
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    ACS Applied Materials & Interfaces

    Cite this: ACS Appl. Mater. Interfaces 2019, 11, 4, 3753–3762
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    https://doi.org/10.1021/acsami.8b20501
    Published January 4, 2019
    Copyright © 2019 American Chemical Society
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    Abstract

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    Biosensors are easy-to-use and cost-effective devices that are emerging as an attractive tool, not only in settling diagnosis or in disease monitoring, but also in mass screening tests, a timely topic that impacts on daily life of the whole society. Nanotechnologies lend themselves to the development of highly sensitive devices whose realization has become a very interdisciplinary topic. Relying on the enhancement of the fluorescence signal detected at the surface of patterned gold nanoparticles, we report the behavior of an analytical device in detecting immunoglobulins in real urine samples that shows a limit of detection of approximately 8 μg/L and a linear range of 10–100 μg/L well below the detection limit of nephelometric method, which is the reference method for this analysis. These performances have been reached thanks to an effective surface functionalization technique and can be improved even more if superydrophobic features of the substrate we produce will be exploited. Since the analyte recognition is realized by antibodies the specificity is very high and, in fact, no interference has been detected by other compounds also present in the real urine samples. The device has been assessed on serum samples by comparing IgG concentrations values obtained by the biosensor with those provided by a nephelometer. In this step we found that our approach allows the analysis of the whole blood without any pretreatment; moreover, it is inherently extendable to the analysis of most biochemical markers in biological fluids.

    Copyright © 2019 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsami.8b20501.

    • Expected contact angle of a superhydrophobic surface (S1). Fluorescence lifetime measurements and imaging (S2). Substrate nanostructure optimization (S3). QCM check of the correct surface functionalization of the flat gold electrode with human IgG (S4). FEM simulations of MEF on the gold flat surface (S5). MEF optimization of the geometry of the gold-nanoparticles (S6–S7). Fluidic circuit (S8). Calibration of the detector (S9). Practical advantages of a superhydrophobic surface in the analysis of analytes (S10). 2D numerical model and simulations (S11–S12). IgG concentrations determined by biosensor in urine samples with and without potential interfering compounds (Table S1). IgG concentration determined by biosensor in a urine sample with low proteinuria (Table S2) (PDF)

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    Cited By

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    This article is cited by 48 publications.

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    ACS Applied Materials & Interfaces

    Cite this: ACS Appl. Mater. Interfaces 2019, 11, 4, 3753–3762
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsami.8b20501
    Published January 4, 2019
    Copyright © 2019 American Chemical Society
    Request reuse permissions

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