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Gold Nanoparticle-Functionalized Reverse Thermal Gel for Tissue Engineering Applications

  • Brisa Peña
    Brisa Peña
    Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States
    Bioengineering Department, University of Colorado Denver Anschutz Medical Campus, Bioscience 2 1270 E. Montview Avenue, Suite 100, Aurora, Colorado 80045, United States
    More by Brisa Peña
  • Marcos Maldonado
    Marcos Maldonado
    Department of Chemistry and Biochemistry, Metropolitan State University of Denver, 1201 5th Street, Denver, Colorado 80206, United States
  • Andrew J. Bonham
    Andrew J. Bonham
    Department of Chemistry and Biochemistry, Metropolitan State University of Denver, 1201 5th Street, Denver, Colorado 80206, United States
  • Brian A. Aguado
    Brian A. Aguado
    Department of Chemical and Biological Engineering and the BioFrontiers Institute, University of Colorado at Boulder, 3415 Colorado Avenue, Boulder, Colorado 80309, United States
  • Antonio Dominguez-Alfaro
    Antonio Dominguez-Alfaro
    Carbon Nanobiotechnology Laboratory CIC biomaGUNE, Paseo de Miramón 182, Donostia-San Sebastián 20009, Spain
    POLYMAT, University of the Basque Country UPV/EHU, Avenida de Tolosa 72, 20018 Donostia-San Sebastián, Spain
  • Melissa Laughter
    Melissa Laughter
    Bioengineering Department, University of Colorado Denver Anschutz Medical Campus, Bioscience 2 1270 E. Montview Avenue, Suite 100, Aurora, Colorado 80045, United States
  • Teisha J. Rowland
    Teisha J. Rowland
    Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States
  • James Bardill
    James Bardill
    Bioengineering Department, University of Colorado Denver Anschutz Medical Campus, Bioscience 2 1270 E. Montview Avenue, Suite 100, Aurora, Colorado 80045, United States
  • Nikki L. Farnsworth
    Nikki L. Farnsworth
    Bioengineering Department, University of Colorado Denver Anschutz Medical Campus, Bioscience 2 1270 E. Montview Avenue, Suite 100, Aurora, Colorado 80045, United States
    Department of Pediatrics, University of Colorado Denver, Anschutz Medical Campus, 1775 Aurora Ct., Bldg. M20, Aurora, Colorado 80045, United States
  • Nuria Alegret Ramon
    Nuria Alegret Ramon
    Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States
    POLYMAT, University of the Basque Country UPV/EHU, Avenida de Tolosa 72, 20018 Donostia-San Sebastián, Spain
  • Matthew R. G. Taylor
    Matthew R. G. Taylor
    Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States
  • Kristi S. Anseth
    Kristi S. Anseth
    Department of Chemical and Biological Engineering and the BioFrontiers Institute, University of Colorado at Boulder, 3415 Colorado Avenue, Boulder, Colorado 80309, United States
  • Maurizio Prato
    Maurizio Prato
    Carbon Nanobiotechnology Laboratory CIC biomaGUNE, Paseo de Miramón 182, Donostia-San Sebastián 20009, Spain
    Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via Giorgieri 1, Trieste 34127, Italy
    Basque Fdn Sci, Ikerbasque, Bilbao 48013, Spain
  • Robin Shandas
    Robin Shandas
    Bioengineering Department, University of Colorado Denver Anschutz Medical Campus, Bioscience 2 1270 E. Montview Avenue, Suite 100, Aurora, Colorado 80045, United States
  • Timothy A. McKinsey
    Timothy A. McKinsey
    Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States
    Consortium for Fibrosis Research & Translation, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, United States
  • Daewon Park*
    Daewon Park
    Bioengineering Department, University of Colorado Denver Anschutz Medical Campus, Bioscience 2 1270 E. Montview Avenue, Suite 100, Aurora, Colorado 80045, United States
    *E-mail: [email protected]. Phone: 303-724-6947 (D.P.).
    More by Daewon Park
  • , and 
  • Luisa Mestroni*
    Luisa Mestroni
    Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, 12700 E. 19th Avenue, Bldg. P15, Aurora, Colorado 80045, United States
    *E-mail: [email protected]. Phone: 303-724-0858 (L.M.).
Cite this: ACS Appl. Mater. Interfaces 2019, 11, 20, 18671–18680
Publication Date (Web):April 25, 2019
https://doi.org/10.1021/acsami.9b00666
Copyright © 2019 American Chemical Society

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    Abstract

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    Utilizing polymers in cardiac tissue engineering holds promise for restoring function to the heart following myocardial infarction, which is associated with grave morbidity and mortality. To properly mimic native cardiac tissue, materials must not only support cardiac cell growth but also have inherent conductive properties. Here, we present an injectable reverse thermal gel (RTG)-based cardiac cell scaffold system that is both biocompatible and conductive. Following the synthesis of a highly functionalizable, biomimetic RTG backbone, gold nanoparticles (AuNPs) were chemically conjugated to the backbone to enhance the system’s conductivity. The resulting RTG–AuNP hydrogel supported targeted survival of neonatal rat ventricular myocytes (NRVMs) for up to 21 days when cocultured with cardiac fibroblasts, leading to an increase in connexin 43 (Cx43) relative to control cultures (NRVMs cultured on traditional gelatin-coated dishes and RTG hydrogel without AuNPs). This biomimetic and conductive RTG–AuNP hydrogel holds promise for future cardiac tissue engineering applications.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsami.9b00666.

    • AuNPs-COOH are well-dispersed in water; the RTG–AuNP system presents significantly higher G′ to the RTH-lysine system (PDF)

    • RTG-lysine hydrogel injection at 37 °C using a 31-gauge needle. The injection is made in water. Polymer concentration: 3% (w/w) (AVI)

    • RTG–AuNP hydrogel injection at 37 °C using a 31-gauge needle. The injection is made in water. Polymer concentration: 3% (w/w) (AVI)

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    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

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    10. Raju Kumar, Avinash Parashar. Atomistic simulations of pristine and nanoparticle reinforced hydrogels: A review. WIREs Computational Molecular Science 2023, 13 (4) https://doi.org/10.1002/wcms.1655
    11. Darryl A. Dickerson. Advancing Engineered Heart Muscle Tissue Complexity with Hydrogel Composites. Advanced Biology 2023, 7 (5) https://doi.org/10.1002/adbi.202200067
    12. Patrícia Severino, Fabio Rocha Formiga, Juliana C. Cardoso, Ricardo L.C. de Albuquerque-Júnior, Marco V. Chaud, Eliana B. Souto. Electrically conductive nanomaterials for advanced cardiac tissue regeneration. 2023, 529-549. https://doi.org/10.1016/B978-0-323-90471-1.00009-8
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    14. Shanghui Huang, Xiangqian Hong, Mingyi Zhao, Nanbo Liu, Huiling Liu, Jun Zhao, Longquan Shao, Wei Xue, Han Zhang, Ping Zhu, Rui Guo. Nanocomposite hydrogels for biomedical applications. Bioengineering & Translational Medicine 2022, 7 (3) https://doi.org/10.1002/btm2.10315
    15. Xu Yan, Huan Sun, Ping Yang, . Recent Advances on Electroconductive Hydrogels Used in Heart Repair and Regeneration. Advances in Materials Science and Engineering 2022, 2022 , 1-13. https://doi.org/10.1155/2022/6042137
    16. Nohra E. Beltran-Vargas, Eduardo Peña-Mercado, Concepción Sánchez-Gómez, Mario Garcia-Lorenzana, Juan-Carlos Ruiz, Izlia Arroyo-Maya, Sara Huerta-Yepez, José Campos-Terán. Sodium Alginate/Chitosan Scaffolds for Cardiac Tissue Engineering: The Influence of Its Three-Dimensional Material Preparation and the Use of Gold Nanoparticles. Polymers 2022, 14 (16) , 3233. https://doi.org/10.3390/polym14163233
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    18. Chen Gao, Shaoshuai Song, Yinjuan Lv, Jie Huang, Zhijun Zhang. Recent Development of Conductive Hydrogels for Tissue Engineering: Review and Perspective. Macromolecular Bioscience 2022, 22 (8) https://doi.org/10.1002/mabi.202200051
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    40. Jian Cao, Zhongxing Liu, Limin Zhang, Jinlong Li, Haiming Wang, Xiuhui Li, . Advance of Electroconductive Hydrogels for Biomedical Applications in Orthopedics. Advances in Materials Science and Engineering 2021, 2021 , 1-13. https://doi.org/10.1155/2021/6668209
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