Time-Resolved Study on Self-Assembling Behavior of PEGylated Gold Nanoparticles in the Presence of Human Serum Albumin: A System for Nanomedical ApplicationsClick to copy article linkArticle link copied!
- Neda Iranpour AnarakiNeda Iranpour AnarakiCenter for X-ray Analytics, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, St. Gallen 9014, SwitzerlandLaboratory of Particles-Biology Interactions, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, St. Gallen 9014, SwitzerlandDepartment of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg 1700, SwitzerlandMore by Neda Iranpour Anaraki
- Marianne LiebiMarianne LiebiCenter for X-ray Analytics, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, St. Gallen 9014, SwitzerlandMore by Marianne Liebi
- Kamran IranshahiKamran IranshahiLaboratory for Biomimetic Membranes and Textiles, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, SwitzerlandDepartment of Environmental Systems Science, Swiss Federal Institute of Technology, ETH-Zurich, Zurich 8092, SwitzerlandMore by Kamran Iranshahi
- Clément BlanchetClément BlanchetEuropean Molecular Biology Laboratory, Notkestrasse 85, Hamburg 22603, GermanyMore by Clément Blanchet
- Peter WickPeter WickLaboratory of Particles-Biology Interactions, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, St. Gallen 9014, SwitzerlandMore by Peter Wick
- Antonia Neels*Antonia Neels*Email: [email protected]Center for X-ray Analytics, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, St. Gallen 9014, SwitzerlandDepartment of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg 1700, SwitzerlandMore by Antonia Neels
Abstract
The combination of a microfluidic approach for synchrotron-based dynamic (early structural changes) with lab-based static small-angle X-ray scattering (SAXS) measurements (longer time scale) allows qualifying nanoparticle (NP) systems for their use in nanomedicine. Time-resolved in situ investigations are performed on self-assembly and colloidal behavior of 5 nm PEGylated (polyethylene glycol) gold NPs in different media. SAXS methods combined with a micromixing fluidic system are used to observe the early stage of NP interactions. Dynamic measurements cover a time range from 1 to 100 s after mixing thoroughly, while static measurements complete the study for up to 10 days after sample preparation. These NPs, after mixing with saline solution (0.9% NaCl solution), self-assemble in 3D ordered domains. The NPs also show this ordering in the presence of human serum albumin (HSA) molecules. It is shown that, although the presence of protein molecules slows down the NP self-assembling process, these molecules improve the long-term colloidal stability of the ordered domains probably via interpolymer complexation between PEG and HSA molecules.
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