Au@Ag Core–Shell Nanoparticles for Colorimetric and Surface-Enhanced Raman-Scattering-Based Multiplex Competitive Lateral Flow Immunoassay for the Simultaneous Detection of Histamine and Parvalbumin in FishClick to copy article linkArticle link copied!
- Carlos Fernández-LodeiroCarlos Fernández-LodeiroCINBIO, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainDepartment of Physical Chemistry, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainGalicia Sur Health Research Institute (IIS Galicia Sur), 36310 Vigo, SpainMore by Carlos Fernández-Lodeiro
- Lara González-CabaleiroLara González-CabaleiroCINBIO, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainDepartment of Physical Chemistry, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainGalicia Sur Health Research Institute (IIS Galicia Sur), 36310 Vigo, SpainMore by Lara González-Cabaleiro
- Lorena Vázquez-IglesiasLorena Vázquez-IglesiasCINBIO, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainDepartment of Physical Chemistry, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainGalicia Sur Health Research Institute (IIS Galicia Sur), 36310 Vigo, SpainMore by Lorena Vázquez-Iglesias
- Esther Serrano-PertierraEsther Serrano-PertierraDepartment of Biochemistry and Molecular Biology and Institute of Biotechnology of Asturias, University of Oviedo, 33006 Oviedo, SpainMore by Esther Serrano-Pertierra
- Gustavo BodelónGustavo BodelónCINBIO, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainDepartment of Functional Biology and Health Sciences, Universidade de Vigo, 36310 Vigo, SpainMore by Gustavo Bodelón
- Mónica CarreraMónica CarreraDepartment of Food Technology, Spanish National Research Council, Marine Research Institute, 36208 Vigo, SpainMore by Mónica Carrera
- María Carmen Blanco-LópezMaría Carmen Blanco-LópezDepartment of Physical and Analytical Chemistry and Institute of Biotechnology of Asturias, University of Oviedo, c/Julián Clavería 8, 33006 Oviedo, SpainMore by María Carmen Blanco-López
- Jorge Pérez-Juste*Jorge Pérez-Juste*Email: [email protected]CINBIO, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainDepartment of Physical Chemistry, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainGalicia Sur Health Research Institute (IIS Galicia Sur), 36310 Vigo, SpainMore by Jorge Pérez-Juste
- Isabel Pastoriza-Santos*Isabel Pastoriza-Santos*Email: [email protected]CINBIO, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainDepartment of Physical Chemistry, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, SpainGalicia Sur Health Research Institute (IIS Galicia Sur), 36310 Vigo, SpainMore by Isabel Pastoriza-Santos
Abstract
Foodborne allergies and illnesses represent a major global health concern. In particular, fish can trigger life-threatening food allergic reactions and poisoning effects, mainly caused by the ingestion of parvalbumin toxin. Additionally, preformed histamine in less-than-fresh fish serves as a toxicological alert. Consequently, the analytical assessment of parvalbumin and histamine levels in fish becomes a critical public health safety measure. The multiplex detection of both analytes has emerged as an important issue. The analytical detection of parvalbumin and histamine requires different assays; while the determination of parvalbumin is commonly carried out by enzyme-linked immunosorbent assay, histamine is analyzed by high-performance liquid chromatography. In this study, we present an approach for multiplexing detection and quantification of trace amounts of parvalbumin and histamine in canned fish. This is achieved through a colorimetric and surface-enhanced Raman-scattering-based competitive lateral flow assay (SERS-LFIA) employing plasmonic nanoparticles. Two distinct SERS nanotags tailored for histamine or β-parvalbumin detection were synthesized. Initially, spherical 50 nm Au@Ag core–shell nanoparticles (Au@Ag NPs) were encoded with either rhodamine B isothiocyanate (RBITC) or malachite green isothiocyanate (MGITC). Subsequently, these nanoparticles were bioconjugated with anti-β-parvalbumin and antihistamine, forming the basis for our detection and quantification methodology. Additionally, our approach demonstrates the use of SERS-LFIA for the sensitive and multiplexed detection of parvalbumin and histamine on a single test line, paving the way for on-site detection employing portable Raman instruments.
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License Summary*
You are free to share(copy and redistribute) this article in any medium or format and to adapt(remix, transform, and build upon) the material for any purpose, even commercially within the parameters below:
Creative Commons (CC): This is a Creative Commons license.
Attribution (BY): Credit must be given to the creator.
*Disclaimer
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License Summary*
You are free to share(copy and redistribute) this article in any medium or format and to adapt(remix, transform, and build upon) the material for any purpose, even commercially within the parameters below:
Creative Commons (CC): This is a Creative Commons license.
Attribution (BY): Credit must be given to the creator.
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Introduction
Scheme 1
aTwo well-differentiated SERS tags conjugated with anti-β-parvalbumin (αParv) and anti-histamine (αHist) are synthesized and mixed with a canned tuna extract. Subsequently, a dual colorimetric SERS-based detection and discrimination of the antigens is performed.
Results and Discussion
Synthesis and Analysis of SERS Tags
Figure 1
Figure 1. SERS tag characterization. (A) Representative TEM image of spherical Au@Ag core–shell nanoparticles (Au@Ag NPs). (B) Size distribution histogram of Au@Ag NPs. (C) SERS spectra of the SERS tags encoded with MGITC (green) and RBITC (red). The green and red shadowed regions indicate 1616 and 1646 cm–1 Raman peaks of MGITC and RBITC-encoded SERS tags, respectively. (D) Normalized extinction spectra of Au@Ag NPs before (black) and after functionalization with RBITC and anti-β-parvalbumin (red line) and with MGITC and antihistamine (green line). The inset clearly shows the red shift in the plasmon band peak after the codification of Au@Ag NPs.
Figure 2
Figure 2. Nanoprobe cross-reactivity assessment. (A) Photograph of the LFIA strip with histamine (Hist), parvalbumin (Parv), and protein-G (PG) immobilized in the test (T) and control (C) lines, as indicated. SERS intensity mappings acquired at 1616 and 1646 cm–1 which are characteristic peaks of αHist-MGITC SERS or αParv-RBITC SERS tags, respectively. (B) Average SERS spectra from the 20 highest intensity points measured in each line. The green and red shadowed regions indicate 1616 and 1646 cm–1 Raman peaks of the MGITC and RBITC-encoded tags. Scale bars in (A) represent 1 mm. Scale bars in (B) represent 1 Kcts mW–1 s–1. All SERS measurements were carried out with a 532 nm laser line, 10× objective, 0.25 mW laser power, acquisition time 0.5 s, and 231 points.
Development of the Competitive SERS-Based LFIA for Detection of Histamine and Parvalbumin
Figure 3
Figure 3. (A and B) Photographs of LFIA strips after running αParv-RBITC SERS tags and αHist- MGITC SERS tags previously incubated with different concentrations of (A) histamine (from 2.5 to 5 × 10–6 mg mL–1) or (B) parvalbumin (from 0.5 to 2.5 × 10–4 mg mL–1) in PB. (C and D) Average SERS spectra acquired from the different Hist (C) and Parv (D) T lines are shown in (A) and (B), respectively. (E, F) Variation of SERS intensity at 1646 cm–1 (E) or 1616 cm–1 (F) with the concentration of parvalbumin and histamine, respectively. The red lines represent the fitting of the SERS intensity measurements to a four-parameter sigmoid equation. Standard deviations correspond to the 20 higher-intensity SERS points of each strip. All SERS measurements were carried out with a 532 nm laser line, 10× objective, 0.25, 2.31, or 12.50 mW laser power depending on the color intensity of the test lines, 1.0 s acquisition time, and 143 points.
antigen | A1 | A2 | X0 (mg mL–1) | p | R2 | IC10/LOD (mg mL–1) | IC20 (mg mL–1) | IC80 (mg mL–1) |
---|---|---|---|---|---|---|---|---|
parvalbumin | 8822.3 ± 297.4 | 358.3 ± 391.2 | 0.021 ± 0.002 | 2.22 ± 0.50 | 0.979 | 7.74 × 10–3 | 1.12 × 10–2 | 3.90 × 10–2 |
histamine | 4113.9 ± 182.6 | 23.2 ± 120.0 | (8.9 ± 1.6)×10–4 | 0.83 ± 0.11 | 0.984 | 6.29 × 10–5 | 1.67 × 10–4 | 4.73 × 10–3 |
Quantitative Detection of Spiked Histamine and Parvalbumin in Canned Tuna by a Dual Colorimetric SERS-LFIA
Figure 4
Figure 4. (A) Photograph of an LFIA strip with a control (C) line and two test lines for parvalbumin (T Parv) and histamine (T Hist), as indicated, and representative SERS spectra measured in each T line of the LFIA strip with a hand-held Raman spectrometer with a 532 nm laser line, 21 mW laser power, and 1.0 s acquisition time. The scale bar represents 5 Kcts mW–1 s–1. (B, C) Optical sensor linear regression range of parvalbumin (B) and histamine (C) obtained by analyzing extracts of canned tuna.
spiked (mg/kg) | found (mg/kg) | recovery(%) | |
---|---|---|---|
histamine | 1 | 0.85 | 85.0 |
5 | 4.6 | 92.6 | |
10 | 9.8 | 98.5 | |
15 | 13.8 | 92.3 | |
20 | 22 | 109.5 | |
50 | 50 | 100.4 | |
parvalbumin | 20 | 28 | 121.4 |
50 | 48 | 111.8 | |
100 | 88 | 109.8 | |
150 | 170 | 111.0 | |
200 | 192 | 88.4 |
Multiplexed SERS Detection in a Single Test Line of Spiked Histamine and Parvalbumin in Canned Tuna
Figure 5
Figure 5. (A) Photograph of four LFIA strips corresponding to experiments performed in the absence of histamine and parvalbumin (1), the presence of histamine and parvalbumin in excess (2), the presence of parvalbumin and no histamine (3) and the presence of histamine and no parvalbumin (4) immobilized in the test (T) line. (B) Representative SERS spectra measured with a hand-held Raman in the different T lines, as indicated. SERS measurements were performed with a 532 nm laser line, 21 mW and 1 s acquisition time. The shadowed regions indicate characteristic Raman peaks of αHist-MGITC SERS tags (1616 cm–1, green) and αParv-RBITC SERS tags (1646 cm–1, in red). The scale bar represents 0.5 Kcts mW–1 s–1. (C) SERS intensity mappings acquired at 1616 cm–1 (left) and 1646 cm–1 (right) in the different T lines from (A), as indicated, showing the presence/absence and spatial distribution of αParv-RBITC SERS tags and αHist-MGITC SERS tags, respectively. Scale bars are 1 mm. SERS mappings were carried out with a 532 nm laser line, 10× objective, 2.31 or 12.50 mW laser power depending on the color intensity of the test lines, acquisition time 1.0 s, and 231 points.
Conclusions
Experimental Section
Materials
Instrumentation
Methods
Synthesis of Citrate-Stabilized Au@Ag NPs
Synthesis of 14.0 nm Au Seeds
Synthesis of Au@Ag Core–Shell NPs
Fabrication of SERS-Encoded NPs
Conjugation of SERS-Encoded NPs with Histamine and Parvalbumin Antibodies
Parvalbumin Protein Extraction
LFIA Strip Fabrication
Histamine and Parvalbumin Calibration Curve Procedure
Canned Tuna Fish Sample Preparation and Test in the Two-Test-Line Sensor
Canned Tuna Fish Sample Test in the Single-Test-Line Sensor
Data Availability
The data that support the findings of this study are available at ZENODO, doi:10.5281/zenodo.10036362.
Supporting Information
The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsanm.3c04696.
SERS spectra of the SERS tags and their assignments; optimization of running buffers and pHs; calibration curves; and fittings for parvalbumin and histamine (PDF)
Terms & Conditions
Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.
Acknowledgments
The authors acknowledge financial support from the European Innovation Council (Horizon 2020 Project: 965018-BIOCELLPHE), the MCIN/AEI/10.13039/501100011033 (grant PID2019-108954RB-I00 and PID2019-103845RB-C21), the FSE (“El FSE invierte en tu futuro”), the Xunta de Galicia/FEDER (grant GRC ED431C 2020/09), the European Regional Development Fund (ERDF), and Consejería de Educación y Ciencia del Principado de Asturias (grant ref. SV-PA-21-AYUD/2021/52132). C.F.-L. and L.G.-C. acknowledge Xunta de Galicia for a predoctoral scholarship (Programa de axudas á etapa predoutoral da Consellería de Cultura, Educación e Universidades da Xunta de Galicia, reference number: 2022/294). Funding for open access by the UniversidadedeVigo/CISUG.
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- 9Feng, C.; Teuber, S.; Gershwin, M. E. Histamine (Scombroid) Fish Poisoning: A Comprehensive Review. Clin Rev. Allergy Immunol 2016, 50 (1), 64– 69, DOI: 10.1007/s12016-015-8467-xGoogle Scholar9https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2MXhvFGksro%253D&md5=d7183abbc805b94eb0873f0712f83effHistamine (Scombroid) Fish Poisoning: a Comprehensive ReviewFeng, Charles; Teuber, Suzanne; Gershwin, M. EricClinical Reviews in Allergy & Immunology (2016), 50 (1), 64-69CODEN: CRAIF2; ISSN:1080-0549. (Springer)A review. Histamine fish poisoning, also known as scombroid poisoning, is the most common cause of ichythyotoxicosis worldwide and results from the ingestion of histamine-contaminated fish in the Scombroidae and Scomberesocidae families, including mackerel, bonito, albacore, and skipjack. This disease was first described in 1799 in Britain and re-emerged in the medical literature in the 1950s when outbreaks were reported in Japan. The symptoms assocd. with histamine fish poisoning are similar to that of an allergic reaction. In fact, such histamine-induced reactions are often misdiagnosed as IgE-mediated fish allergy. Indeed, histamine fish poisoning is still an underrecognized disease. In this review, we discuss the epidemiol., pathophysiol., evaluation, and treatment of scombroid disease. Because more than 80 % of fish consumed in the USA is now imported from other countries, the disease is intimately linked with the global fish trade (National Marine Fisheries Service, 2012). Preventing future scombroid outbreaks will require that fishermen, public health officials, restaurant workers, and medical professionals work together to devise international safety stds. and increase awareness of the disease. The implications of scombroid poisoning go far beyond that of fish and have broader implications for the important issues of food safety.
- 10Schirone, M.; Visciano, P.; Tofalo, R.; Suzzi, G.; Hattori, Y.; Seifert, R.; Schirone, M.; Visciano, P.; Tofalo, R.; Suzzi, G. Histamine Food Poisoning. In Histamine and Histamine Receptors in Health and Disease; Springer: Cham, 2016; pp. 217– 235.Google ScholarThere is no corresponding record for this reference.
- 11Zhao, Y.; Zhang, X.; Jin, H.; Chen, L.; Ji, J.; Zhang, Z. Histamine Intolerance─A Kind of Pseudoallergic Reaction. Biomolecules 2022, 12 (3), 454, DOI: 10.3390/biom12030454Google Scholar11https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XoslKlt74%253D&md5=8454ac794eb199a401e299dec264edf2Histamine Intolerance-A Kind of Pseudoallergic ReactionZhao, Ying; Zhang, Xiaoyan; Jin, Hengxi; Chen, Lu; Ji, Jiang; Zhang, ZhongweiBiomolecules (2022), 12 (3), 454CODEN: BIOMHC; ISSN:2218-273X. (MDPI AG)A review. Histamine intolerance (HIT) is a common disorder assocd. with impaired histamine metab. Notwithstanding, it is often misdiagnosed as other diseases because of its lack of specific clin. manifestations. HIT did not gain traction until the early 21st century. In this review, we will focus on the latest research and elaborate on the clin. manifestations of HIT, including its manifestations in special populations such as atopic dermatitis (AD) and chronic urticaria (CU), as well as the latest understanding of its etiol. and pathogenesis. In addn., we will explore the latest treatment strategies for HIT and the treatment of specific cases.
- 12Chen, X.; Zhang, D.; Liu, Q.; Liu, S.; Li, H.; Li, Z. Enzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne Allergens. Anal. Chem. 2023, 95 (18), 7354– 7362, DOI: 10.1021/acs.analchem.3c00591Google Scholar12https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXosVaks70%253D&md5=6e4081a47a37f81a66ebc8da83b3166aEnzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne AllergensChen, Xiaofeng; Zhang, Dongdong; Liu, Qingmei; Liu, Sihui; Li, Houlin; Li, ZhengAnalytical Chemistry (Washington, DC, United States) (2023), 95 (18), 7354-7362CODEN: ANCHAM; ISSN:0003-2700. (American Chemical Society)Food allergy has become a growing health concern that may impair life quality and even cause life-threatening outcomes. Accidental and continuous exposure to allergenic bioaerosols has a substantially neg. impact on the respiratory health of patients. Traditional anal. methodologies for food allergens are restricted by strong reliance on bulk instrumentation and skilled personnel, particularly in low-resource settings. In this study, a fluorescent sensor array based on the ELISA performed on a herringbone-shaped microfluidic chip (ELISA-HB-chip) was designed for dynamically sensitive and multiplexed quantification of foodborne allergens in aerosols that originated from liq. food exts. Due to the high surface area of aerosol particles and sufficient mixing of immunol. reagents using a herringbone micromixer, the detection sensitivity was improved by over an order of magnitude compared to traditional allergen detection in the aq. phase. Through fluorescence imaging of multiple regions on the ELISA-HB-chip, four important foodborne allergens, namely, ovalbumin, ovomucoid, lysozyme, and tropomyosin, could be simultaneously monitored without any cross-reactivity, and the limits of detection for these allergenic species were detd. to be 7.8, 1.2, 4.2, and 0.31 ng/mL, resp. Combining with a 3D printed and portable fluorescence microscope, this platform exhibited an excellent field-deployable capacity for quick and accurate detn. of allergens in the aerosol state from spiked buffer solns., thus displaying the practicality for food safety screening at cooking or food processing sites where patients are potentially under exposure to allergenic bioaerosols that escaped from food matrixes or exts.
- 13Munir, M. A.; Mackeen, M. M. M.; Heng, L. Y.; Badri, K. H. Study of Histamine Detection Using Liquid Chromatography and Gas Chromatography. ASM Science Journal 2021, 16, 1– 9, DOI: 10.32802/asmscj.2021.809Google ScholarThere is no corresponding record for this reference.
- 14Zhang, B.; Cai, D.; Lang, Y.; Lin, X.; Yang, K.; Shentu, X.; Yu, X. A Smartphone-Integrated Multi-Model Thermal Immunochromatographic Assay for Sensitive Detection of Histamine in Real Samples. Sens Actuators B Chem. 2023, 394, 134474 DOI: 10.1016/j.snb.2023.134474Google Scholar14https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhsleku7fN&md5=13456e2fe1ea08a6c2ae3eea3cd6afebA smartphone-integrated multi-model thermal immunochromatographic assay for sensitive detection of histamine in real samplesZhang, Biao; Cai, Danfeng; Lang, Yihan; Lin, Xiaodong; Yang, Ke; Shentu, Xuping; Yu, XiaopingSensors and Actuators, B: Chemical (2023), 394 (), 134474CODEN: SABCEB; ISSN:0925-4005. (Elsevier B.V.)A smartphone-integrated multi-model thermal immunochromatog. assay has been constructed for the detection of histamine by using anti-histamine antibody coupled Cu2-xSe nanoparticles (Cu2-xSe@antibody) as photothermal nanoprobes. Base on a competitive format, the coated histamine antigen on T-line and histamine simultaneously competed for binding the lateral flow photothermal signal probes. With the increase of histamine concn., the temp. of Cu2-xSe@antibody on T-line was decreased under the 808 nm laser irradn., which can be monitored via the smartphone-thermal imager combination device. In addn., the color on T-line from nanoprobes gradually disappeared, and the inhibition rate calcd. from the obtained temp. on T-line was increased. The smartphone-integrated multi-model thermal immunochromatog. assay enables detection of histamine in the range of 0.1-10.0μg/L with limit of detection as low as 0.09μg/L. The histamine concns. in crab, tuna, saury, mackerel, shrimp were 5.67 mg/kg, 83.73 mg/kg, 14.03 mg/kg, 21.30 mg/kg, 3.22 mg/kg detected by the developed thermal immunochromatog. assay, resp. The developed multi-model strategy provided good sensitivity, simple operation, effectiveness and practicability for histamine in real samples compared to ultra-performance liq. chromatog.-tandem mass spectrometry UPLC-MS/MS and ELISA.
- 15Xu, L.; Zhou, J.; Eremin, S.; Dias, A. C. P.; Zhang, X. Development of ELISA and Chemiluminescence Enzyme Immunoassay for Quantification of Histamine in Drug Products and Food Samples. Anal Bioanal Chem. 2020, 412, 4739– 4747, DOI: 10.1007/s00216-020-02730-5Google Scholar15https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXhtVOksL%252FF&md5=f5812a1014fdb3d27029be4118c6f0c7Development of ELISA and chemiluminescence enzyme immunoassay for quantification of histamine in drug products and food samplesXu, Long; Zhou, Jiping; Eremin, Sergei; Dias, Alberto C. P.; Zhang, XiaoyingAnalytical and Bioanalytical Chemistry (2020), 412 (19), 4739-4747CODEN: ABCNBP; ISSN:1618-2642. (Springer)Histamine (HA) is a biogenic amine assocd. with allergies and food poisoning. It is an important indicator of food freshness and quality. In recent years, a series of medical negligence cases have been reported to be related to the i.v. injection of antibiotics produced via fermn. with fish peptone due to HA contamination. To detect HA efficiently, mouse monoclonal antibody was developed. An ELISA and a chemiluminescence enzyme immunoassay (CLEIA) were developed and compared with conventional HPLC anal. Both immunoassays showed low cross-reactivity, low 50% inhibitive concn. (IC50; 1.2μg/mL and 1.1μg/mL), low limits of detection (LODs, IC10; 89.0 ng/mL and 73.4 ng/mL), and appreciable recoveries in spiked foods and drugs (from 73.4 to 131.0% and from 77.0 to 119.0%, espectively), demonstrating that the developed methods are sensitive, specific, fast, and reliable for HA detection in complicated real samples.
- 16Zhang, M.; Li, M.; Zhao, Y.; Xu, N.; Peng, L.; Wang, Y.; Wei, X. Novel Monoclonal Antibody-Sandwich Immunochromatographic Assay Based on Fe3O4/Au Nanoparticles for Rapid Detection of Fish Allergen Parvalbumin. Food Research International 2021, 142, 110102 DOI: 10.1016/j.foodres.2020.110102Google Scholar16https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXksl2qtr4%253D&md5=9c228941c75036e6cd17103a739fd7afNovel monoclonal antibody-sandwich immunochromatographic assay based on Fe3O4/Au nanoparticles for rapid detection of fish allergen parvalbuminZhang, Mengke; Li, Mengyin; Zhao, Yan; Xu, Naifeng; Peng, Lanlan; Wang, Yuanfeng; Wei, XinlinFood Research International (2021), 142 (), 110102CODEN: FORIEU; ISSN:0963-9969. (Elsevier B.V.)In this study, a rapid sandwich immunochromatog. assay (ICA) was developed to detect parvalbumin (PV). Firstly, two optimum primary monoclonal antibody (mAb) against PV had been screened out: mAb1 was used as the capture antibody, and mAb2 conjugated to Fe3O4/Au nanoparticles (Fe3O4/AuNPs) that served as a detection reagent. Using this pair of mAbs, a sandwich ICA strip based on Fe3O4/AuNPs was developed. The results showed that the color intensity of test line pos. correlated with the PV concn. in the std. or spiked sample. The limit of detection for qual. (LOD) and quant. detection (LOQ) were 2 ng/mL and 0.691 ng/mL, resp. Besides, the detection time of this ICA strip was within 15 min. The recovery rates ranged from 104.0% to 117.4%, within an acceptable level (80-120%). Moreover, the developed assay also showed high cross reaction in different fish species. These results demonstrated that the established test strip has the potential to be used as a rapid screening tool for large scale detn. of PV in foodstuffs.
- 17Cai, Q. F.; Wang, X. C.; Liu, G. M.; Zhang, L.; Ruan, M. M.; Liu, Y.; Cao, M. J. Development of a Monoclonal Antibody-Based Competitive Enzyme Linked-Immunosorbent Assay (c-ELISA) for Quantification of Silver Carp Parvalbumin. Food Control 2013, 29 (1), 241– 247, DOI: 10.1016/j.foodcont.2012.06.016Google Scholar17https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC38XhtlGqt77O&md5=33a6ef5940a93a176cfb03b99ede949dDevelopment of a monoclonal antibody-based competitive enzyme linked-immunosorbent assay (c-ELISA) for quantification of silver carp parvalbuminCai, Qiu-Feng; Wang, Xi-Chang; Liu, Guang-Ming; Zhang, Lin; Ruan, Mi-Mi; Liu, Yuan; Cao, Min-JieFood Control (2013), 29 (1), 241-247CODEN: FOOCEV; ISSN:0956-7135. (Elsevier Ltd.)Parvalbumin (PV) is a major allergen in fish. A monoclonal antibody (B2-E1) against silver carp parvalbumin was prepd. in the present study. Western blot anal. indicated that the prepd. monoclonal antibody was specific to PV in fish muscle ext. A competitive ELISA (c-ELISA) was then developed to quantify the amt. of PV in silver carp using B2-E1. The limit of detection (LOD) and the limit of quantification (LOQ) of the developed c-ELISA were 0.04 and 0.3 mg/kg food, resp. The c-ELISA was specific for detecting PV from fish, and the intra- and inter-assay coeffs. of variation were 4.4% and 8.4%, resp. The c-ELISA method was further applied to quantify PV from various fish species, and demonstrated that the PV content was 4-20 times higher in the white muscle than that in the dark muscle, which was coincident with the previous reports. Moreover, c-ELISA was used to trace the PV during fish surimi (fish ball) prodn., and the results indicated that washing process could effectively but not completely remove PV from fish ball. Our present study indicated that this c-ELISA method may be applied to monitor trace amts. of fish allergen PV during fishery product processing.
- 18Mukherjee, S.; Horka, P.; Zdenkova, K.; Cermakova, E. Parvalbumin: A Major Fish Allergen and a Forensically Relevant Marker. Genes 2023, 14, 223, DOI: 10.3390/genes14010223Google Scholar18https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhvFylt7g%253D&md5=9f9456cfbe43b54438893385e8a516abParvalbumin: A Major Fish Allergen and a Forensically Relevant MarkerMukherjee, Subham; Horka, Petra; Zdenkova, Kamila; Cermakova, EliskaGenes (2023), 14 (1), 223CODEN: GENEG9; ISSN:2073-4425. (MDPI AG)A review. Parvalbumins (PVALBs) are low mol. wt. calcium-binding proteins. In addn. to their role in many biol. processes, PVALBs play an important role in regulating Ca2+ switching in muscles with fast-twitch fibers in addn. to their role in many biol. processes. The PVALB gene family is divided into two gene types, alpha (α) and beta (β), with the β gene further divided into two gene types, beta1 (β1) and beta2 (β2), carrying traces of whole genome duplication. A large variety of commonly consumed fish species contain PVALB proteins which are known to cause fish allergies. More than 95% of all fish-induced food allergies are caused by PVALB proteins. The authentication of fish species has become increasingly important as the seafood industry continues to grow and the growth brings with it many cases of food fraud. Since the PVALB gene plays an important role in the initiation of allergic reactions, it has been used for decades to develop alternate assays for fish identification. A brief review of the significance of the fish PVALB genes is presented in this article, which covers evolutionary diversity, allergic properties, and potential use as a forensic marker.
- 19Saptarshi, S. R.; Sharp, M. F.; Kamath, S. D.; Lopata, A. L. Antibody Reactivity to the Major Fish Allergen Parvalbumin Is Determined by Isoforms and Impact of Thermal Processing. Food Chem. 2014, 148, 321– 328, DOI: 10.1016/j.foodchem.2013.10.035Google Scholar19https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3sXhsl2lsLvI&md5=b3abc39a58395137530f23879a5ca28fAntibody reactivity to the major fish allergen parvalbumin is determined by isoforms and impact of thermal processingSaptarshi, Shruti R.; Sharp, Michael F.; Kamath, Sandip D.; Lopata, Andreas L.Food Chemistry (2014), 148 (), 321-328CODEN: FOCHDJ; ISSN:0308-8146. (Elsevier Ltd.)The EF-hand calcium binding protein, parvalbumin, is a major fish allergen. Detection of this allergen is often difficult due to its structural diversity among various fish species. The aim of this study was to evaluate the cross-reactivity of parvalbumin in a comprehensive range of bony and cartilaginous fish, from the Asia-Pacific region, and conduct a mol. anal. of this highly allergenic protein. Using the monoclonal anti-parvalbumin antibody PARV-19, we demonstrated the presence of monomeric and oligomeric parvalbumin in all fish analyzed, except for gummy shark, a cartilaginous fish. Heat processing of this allergen greatly affected its antibody reactivity. While heating caused a redn. in antibody reactivity to multimeric forms of parvalbumins for most bony fish, a complete loss of reactivity was obsd. for cartilaginous fish. Mol. anal. demonstrated that parvalbumin cross-reactivity, among fish species, is due to the mol. phylogenetic assocn. of this major fish allergen.
- 20Nevado, D. L.; Santos, S. D.; Bastian, G.; Deyta, J.; Managuelod, E. J.; Fortaleza, J. A.; De Jesus, R. Detection, Identification, and Inactivation of Histamine-Forming Bacteria in Seafood: A Mini-Review. J. Food Prot. 2023, 86 (3), 100049 DOI: 10.1016/j.jfp.2023.100049Google Scholar20https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB28bmsFGrtg%253D%253D&md5=6cc04786e61dc780bdbf1e143aded94bDetection, Identification, and Inactivation of Histamine-forming Bacteria in Seafood: A Mini-reviewNevado Daniel Lance; Delos Santos Sophia; Bastian Gelian; Deyta Jimson; Managuelod El-Jay; Fortaleza Jamil Allen; De Jesus RenerJournal of food protection (2023), 86 (3), 100049 ISSN:.Seafood is one of the essential sources of nutrients for the human diet. However, they can be subject to contamination and can cause foodborne illnesses, including scombroid fish poisoning caused by histamine. Many microorganisms can produce enzymes that eventually decompose endogenous histidine to histamine in postmortem fish muscles and tissues. One of these is histamine-forming bacteria (HFB), primarily found in the gills, gut, and skin of fishes. Previous studies linked a plethora of Gram-negative HFB including Morganella spp. and Photobacterium spp. to scombroid fish poisoning from many types of seafood, especially the Scombridae family. These bacteria possess the hdc gene to produce histidine decarboxylase enzyme. It was reported that Gram-negative HFB produced 6345 ppm in tuna and 1223 ppm in Spanish mackerel. Interestingly, Gram-positive HFB have been isolated in the seafood samples with lower histamine levels. It suggests that Gram-negative HFB are the major contributor to the accumulation of histamine in seafood. Several analytical methods are available to detect and identify HFB and their histamine metabolites from seafood substrates. Rapid test kits can be used in food production settings for early detection of histamine to avoid food intoxication. Furthermore, high hydrostatic pressure and irradiation treatment could prevent the proliferation of HFB and inactivate the existing histidine decarboxylase (HDC) activity. As demonstrated in different seafood model systems, the HDC activity was deactivated at a maximum high hydrostatic pressure level of 400 MPa. The complete inactivation of HFB was achieved by gamma irradiation at a dose of 4.0 kGy. Other postharvest treatments, like enzymatic degradation and electrolyzed oxidizing water, were studied as sustainable methods for bacterial growth prevention and enzyme inactivation. However, other HFB react differently to these treatment conditions, and further studies are recommended.
- 21Visciano, P.; Schirone, M.; Tofalo, R.; Suzzi, G. Histamine Poisoning and Control Measures in Fish and Fishery Products. Front Microbiol 2014, 5, 1– 3, DOI: 10.3389/fmicb.2014.00500Google ScholarThere is no corresponding record for this reference.
- 22Kobayashi, Y.; Huge, J.; Imamura, S.; Hamada-Sato, N. Study of the Cross-Reactivity of Fish Allergens Based on a Questionnaire and Blood Testing. Allergology International 2016, 65 (3), 272– 279, DOI: 10.1016/j.alit.2016.01.002Google Scholar22https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2sXitVWlurnF&md5=8689e82073c039890780ebd4c35db6b6Study of the cross-reactivity of fish allergens based on a questionnaire and blood testingKobayashi, Yukihiro; Huge, Jiletu; Imamura, Shintaro; Hamada-Sato, NaokoAllergology International (2016), 65 (3), 272-279CODEN: ALINFR; ISSN:1323-8930. (Japanese Society of Allergology)Background: Parvalbumin and collagen have been identified as cross-reactive allergens for fish allergies. Although doctors realize that various fish elicit allergies, the targets of food allergen labeling laws were only mackerels and salmons in Japan and mackerels in South Korea. This study aimed to reveal the causative species for fish allergy via questionnaires and blood tests. Methods: Questionnaire research was conducted in Japan via the internet concerning allergies for fish-allergic patients or their family members. Next, IgE reactivities and cross-reactivities of 26 fish species were analyzed using sera obtained from 16 Japanese patients who were allergic to fish parvalbumin or collagen by ELISA (ELISA) and inhibition ELISA. Results: Questionnaire research revealed that 88% patients cannot eat mackerel and salmon in addn. to other fish. In addn., 85% respondents were not satisfied with the current food allergen labeling law. In ELISA analyses, we clarified that pooled serum obtained from patients with fish parvalbumin-specific allergies exhibited IgE reactivity to the exts. of most fish species, and pooled serum obtained from patients with fish collagen-specific allergies displayed IgE reactivity to the exts. of all types of fish. Inhibition ELISA expts. revealed cross-reactivities of parvalbumin or collagen to exts. from all fish tested. Conclusions: Most patients with fish allergies displayed allergic symptoms following the intake of various fish species. In addn., fish parvalbumin and collagen were causative factors of fish allergy and were highly cross-reactive fish panallergens. Therefore, current laws should be revised in Japan and South Korea.
- 23Yang, H.; Min, J.; Han, X.-Y.; Li, X.-Y.; Hu, J.-W.; Liu, H.; Cao, M.-J.; Liu, G.-M. Reduction of the Histamine Content and Immunoreactivity of Parvalbumin in Decapterus Maruadsi by a Maillard Reaction Combined with Pressure Treatment. Food Funct. 2018, 9, 4897– 4905, DOI: 10.1039/c8fo01167bGoogle Scholar23https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1cXhsF2jsbbO&md5=6d30682375b7e1045e234f038f0b8365Reduction of the histamine content and immunoreactivity of parvalbumin in Decapterus maruadsi by a Maillard reaction combined with pressure treatmentYang, Huang; Min, Juan; Han, Xin-Yu; Li, Xiao-Yan; Hu, Jia-Wei; Liu, Hong; Cao, Min-Jie; Liu, Guang-MingFood & Function (2018), 9 (9), 4897-4905CODEN: FFOUAI; ISSN:2042-6496. (Royal Society of Chemistry)The aim of this study was to develop an effective method for decreasing the content of histamine and the immunoreactivity of parvalbumin in Decapterus maruadsi. As demonstrated by reverse phase high performance liq. chromatog., no effect on histamine content was found when fish were treated by boiling (100 °C), ultrasonication, UV irradn., pressure treatment (121 °C, 0.12 MPa). However, the histamine content was reduced by 73.55% when the Maillard reaction was combined with pressure treatment (MPT). Further, the allergenicity of parvalbumin was retained after boiling, ultrasonication and UV irradn., but was effectively decreased when fish were treated by MPT. Animal exptl. results showed lower levels of IgE, IgG1 and IgG2a and contents of serum histamine when measured in a group of MPT sensitized mice. These results showed that the MPT is an effective method for simultaneously reducing the histamine content and the immunoreactivity of parvalbumin from Decapterus maruadsi.
- 24Liu, Y.; Zhan, L.; Qin, Z.; Sackrison, J.; Bischof, J. C. Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis. ACS Nano 2021, 15 (3), 3593– 3611, DOI: 10.1021/acsnano.0c10035Google Scholar24https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXksVanurs%253D&md5=de016e660dce04b0e518d73181c2cf90Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care DiagnosisLiu, Yilin; Zhan, Li; Qin, Zhenpeng; Sackrison, James; Bischof, John C.ACS Nano (2021), 15 (3), 3593-3611CODEN: ANCAC3; ISSN:1936-0851. (American Chemical Society)A review. Lateral flow assays (LFAs) are paper-based point-of-care (POC) diagnostic tools that are widely used because of their low cost, ease of use, and rapid format. Unfortunately, traditional com. LFAs have significantly poorer sensitivities (μM) and specificities than std. lab. tests (ELISA: pM-fM; polymerase chain reaction, PCR: aM), thus limiting their impact in disease control. In this Perspective, we review the evolving efforts to increase the sensitivity and specificity of LFAs. Recent work to improve the sensitivity through assay improvement includes optimization of the assay kinetics and signal amplification by either reader systems or addnl. reagents. Together, these efforts have produced LFAs with ELISA-level sensitivities (pM-fM). In addn., sample preamplification can be applied to both nucleic acids (direct amplification) and other analytes (indirect amplification) prior to LFA testing, which can lead to PCR-level (aM) sensitivity. However, these amplification strategies also increase the detection time and assay complexity, which inhibits the large-scale POC use of LFAs. Perspectives to achieve future rapid (<30 min), ultrasensitive (PCR-level), and "sample-to-answer" POC diagnostics are also provided. In the case of LFA specificity, recent research efforts have focused on high-affinity mols. and assay optimization to reduce nonspecific binding. Furthermore, novel highly specific mols., such as CRISPR/Cas systems, can be integrated into diagnosis with LFAs to produce not only ultrasensitive but also highly specific POC diagnostics. In summary, with continuing improvements, LFAs may soon offer performance at the POC that is competitive with lab. techniques while retaining a rapid format.
- 25Sena-Torralba, A.; Álvarez-Diduk, R.; Parolo, C.; Piper, A.; Merkoçi, A. Toward Next Generation Lateral Flow Assays: Integration of Nanomaterials. Chem. Rev. 2022, 122 (18), 14881– 14910, DOI: 10.1021/acs.chemrev.1c01012Google Scholar25https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XitlSlu77J&md5=d0e1f585d8de5c5c880ab849aaf82e3cToward Next Generation Lateral Flow Assays: Integration of NanomaterialsSena-Torralba, Amadeo; Alvarez-Diduk, Ruslan; Parolo, Claudio; Piper, Andrew; Merkoci, ArbenChemical Reviews (Washington, DC, United States) (2022), 122 (18), 14881-14910CODEN: CHREAY; ISSN:0009-2665. (American Chemical Society)A review. Lateral flow assays (LFAs) are currently the most used point-of-care sensors for both diagnostic (e.g., pregnancy test, COVID-19 monitoring) and environmental (e.g., pesticides and bacterial monitoring) applications. Although the core of LFA technol. was developed several decades ago, in recent years the integration of novel nanomaterials as signal transducers or receptor immobilization platforms has brought improved anal. capabilities. In this Review, we present how nanomaterial-based LFAs can address the inherent challenges of point-of-care (PoC) diagnostics such as sensitivity enhancement, lowering of detection limits, multiplexing, and quantification of analytes in complex samples. Specifically, we highlight the strategies that can synergistically solve the limitations of current LFAs and that have proven com. feasibility. Finally, we discuss the barriers toward commercialization and the next generation of LFAs.
- 26Posthuma-Trumpie, G. A.; Korf, J.; van Amerongen, A. Lateral Flow (Immuno)Assay: Its Strengths, Weaknesses, Opportunities and Threats A Literature Survey. Anal Bioanal Chem. 2009, 393 (2), 569– 582, DOI: 10.1007/s00216-008-2287-2Google Scholar26https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BD1cXhsFWit77M&md5=6bfd3157ea4aefdccf24bd45eff6dc09Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature surveyPosthuma-Trumpie, Geertruida A.; Korf, Jakob; van Amerongen, AartAnalytical and Bioanalytical Chemistry (2009), 393 (2), 569-582CODEN: ABCNBP; ISSN:1618-2642. (Springer)A review. Lateral flow (immuno)assays are currently used for qual., semiquant. and to some extent quant. monitoring in resource-poor or non-lab. environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quant. monitoring. We illustrate the potentials and limitations of anal. with lateral flow (immuno)assays using a literature survey and a SWOT anal. (acronym for "strengths, weaknesses, opportunities, threats"). Articles referred to in this survey were searched for on MEDLINE, Scopus and in refs. of reviewed papers. Search terms included "immunochromatog.", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".
- 27Boehringer, H. R.; O’Farrell, B. J. Lateral Flow Assays in Infectious Disease Diagnosis. Clin Chem. 2021, 68 (1), 52– 58, DOI: 10.1093/clinchem/hvab194Google Scholar27https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB2M%252FitFGntQ%253D%253D&md5=5b0b6da270c6c3c97e88b15d6da9a090Lateral Flow Assays in Infectious Disease DiagnosisBoehringer Hans R; O'Farrell Brendan JClinical chemistry (2021), 68 (1), 52-58 ISSN:.BACKGROUND: Lateral flow immunoassays are widely used as diagnostic tests in many applications in human and other diagnostic areas. Assays for human applications have been commercially available since the 1980s and initially were primarily used to identify pregnancy by measuring human chorionic gonadotropin in urine and serum/plasma. CONTENT: The first infectious disease lateral flow assays were commercialized in the late 1980s identifying the presence of Group A Streptococcus pyogenes collected with throat swabs; innumerable other applications followed in the intervening decades. The severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) pandemic has brought a vast number of new assays for which emergency use authorization (EUA) has been requested in the USA. These assays have been designed for detection of the antibody response to an infection and viral antigens in respiratory samples. In view of the onslaught of new tests, this review will focus on the use of rapid lateral flow immunoassays for infectious diseases. Principles of lateral flow assays and approaches to the production of high-sensitivity point-of-care assays are presented. Market trends, customer requirements, and future directions of lateral flow assay technology and its applications in the infectious disease diagnostic space are discussed. SUMMARY: Lateral flow immunoassays play an important role in infectious disease diagnostics. Advancements in technology have led to improved performance of these assays and acceptance by professional users. With the advent of the SARS-CoV-2 pandemic, the market has reached new levels requiring hundreds of millions of tests per year for professional and even home use.
- 28Kim, J.; Shin, M.-S.; Shin, J.; Kim, H.-M.; Pham, X.-H.; Park, S.; Kim, D.-E.; Kim, Y. J.; Jun, B.-H. Recent Trends in Lateral Flow Immunoassays with Optical Nanoparticles. Int. J. Mol. Sci. 2023, 24 (11), 9600, DOI: 10.3390/ijms24119600Google Scholar28https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXht1CnsLvF&md5=3bf6fb2e85b1457adfda9db0be971994Recent Trends in Lateral Flow Immunoassays with Optical NanoparticlesKim, Jaehi; Shin, Min-Sup; Shin, Jonghyun; Kim, Hyung-Mo; Pham, Xuan-Hung; Park, Seung-min; Kim, Dong-Eun; Kim, Young Jun; Jun, Bong-HyunInternational Journal of Molecular Sciences (2023), 24 (11), 9600CODEN: IJMCFK; ISSN:1422-0067. (MDPI AG)A review. Rapid, accurate, and convenient diagnosis is essential for effective disease management. Various detection methods, such as ELISA, have been extensively used, with lateral flow immunoassay (LFIA) recently emerging as a major diagnostic tool. Nanoparticles (NPs) with characteristic optical properties are used as probes for LFIA, and researchers have presented various types of optical NPs with modified optical properties. Herein, we review the literature on LFIA with optical NPs for the detection of specific targets in the context of diagnostics.
- 29Liang, P.; Guo, Q.; Zhao, T.; Wen, C.-Y.; Tian, Z.; Shang, Y.; Xing, J.; Jiang, Y.; Zeng, J. Ag Nanoparticles with Ultrathin Au Shell-Based Lateral Flow Immunoassay for Colorimetric and SERS Dual-Mode Detection of SARS-CoV-2 IgG. Anal. Chem. 2022, 94 (23), 8466– 8473, DOI: 10.1021/acs.analchem.2c01286Google Scholar29https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XhsVagsrvK&md5=34ef55dcb8d60f9c5b1c53ddfec70df6Ag Nanoparticles with Ultrathin Au Shell-Based Lateral Flow Immunoassay for Colorimetric and SERS Dual-Mode Detection of SARS-CoV-2 IgGLiang, Penghui; Guo, Qi; Zhao, Tianyu; Wen, Cong-Ying; Tian, Zhangyu; Shang, Yanxue; Xing, Jinyan; Jiang, Yongzhong; Zeng, JingbinAnalytical Chemistry (Washington, DC, United States) (2022), 94 (23), 8466-8473CODEN: ANCHAM; ISSN:0003-2700. (American Chemical Society)Ig detection is essential for diagnosing progression of SARS-CoV-2 infection, for which SARS-CoV-2 IgG is one of the most important indexes. In this paper, Ag nanoparticles with ultrathin Au shells (∼2 nm) embedded with 4-mercaptobenzoic acid (MBA) (AgMBA@Au) were manufd. via a ligand-assisted epitaxial growth method and integrated into lateral flow immunoassay (LFIA) for colorimetric and SERS dual-mode detection of SARS-CoV-2 IgG. AgMBA@Au possessed not only the surface chem. advantages of Au but also the superior optical characteristics of Ag. Moreover, the nanogap between the Ag core and the Au shell also greatly enhanced the Raman signal. After being modified with anti-human antibodies, AgMBA@Au recognized and combined with SARS-CoV-2 IgG, which was captured by the SARS-CoV-2 spike protein on the T line. Qual. anal. was achieved by visually observing the color of the T line, and quant. anal. was conducted by measuring the SERS signal with a sensitivity 4 orders of magnitude higher (detection limit: 0.22 pg/mL). The intra-assay and inter-assay variation coeffs. were 7.7 and 10.3%, resp., and other proteins at concns. 10-20-fold higher than those of SARS-CoV-2 IgG could hardly produce distinguishable signals, confirming good reproducibility and specificity. Finally, this method was used to detect 107 clin. serum samples. The results agreed well with those obtained from ELISA kits and were significantly better than those of the colloidal gold test strips. Therefore, this dual-mode LFIA has great potential in clin. practical applications and can be used to screen and trace the early immune response of SARS-CoV-2.
- 30Joung, Y.; Kim, K.; Lee, S.; Chun, B.-S.; Lee, S.; Hwang, J.; Choi, S.; Kang, T.; Lee, M.-K.; Chen, L.; Choo, J. Rapid and Accurate On-Site Immunodiagnostics of Highly Contagious Severe Acute Respiratory Syndrome Coronavirus 2 Using Portable Surface-Enhanced Raman Scattering-Lateral Flow Assay Reader. ACS Sens 2022, 7 (11), 3470– 3480, DOI: 10.1021/acssensors.2c01808Google Scholar30https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XivVKjsLbN&md5=6b7ac8e90aac6cf7553a6fdb52f2f301Rapid and Accurate On-Site Immunodiagnostics of Highly Contagious Severe Acute Respiratory Syndrome Coronavirus 2 Using Portable Surface-Enhanced Raman Scattering-Lateral Flow Assay ReaderJoung, Younju; Kim, Kihyun; Lee, Seungwoo; Chun, Byung-Seon; Lee, Sangyeop; Hwang, Joonki; Choi, Suji; Kang, Taejoon; Lee, Mi-Kyung; Chen, Lingxin; Choo, JaebumACS Sensors (2022), 7 (11), 3470-3480CODEN: ASCEFJ; ISSN:2379-3694. (American Chemical Society)In early 2022, the no. of people infected with the highly contagious mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), called Omicron, was increasing worldwide. Therefore, several countries approved the lateral flow assay (LFA) strip as a diagnostic method for confirming SARS-CoV-2 instead of reverse transcription-polymerase chain reaction (RT-PCR), which takes a long time to generate the results. However, owing to the limitation of detection sensitivity, com. LFA strips have high false-neg. diagnosis rates for patients with low virus concns. Therefore, in this study, we developed a portable surface-enhanced Raman scattering (SERS)-LFA reader based on localized surface plasmon effects to solve the sensitivity problem of the com. LFA strip. We tested 54 clin. samples using this portable SERS-LFA reader, which generated 49 pos. and 5 neg. results. Out of the 49 pos. results, SERS-LFA classified only 2 as false neg., while the com. LFA classified 21 as false neg. This confirmed that the false-neg. rate had significantly improved compared to that of com. LFA strips. We believe that the proposed SERS-LFA system can be utilized as a point-of-care diagnostic system to quickly and accurately det. a virus infection that could spread significantly within a short period.
- 31Castrejón-Jiménez, N. S.; García-Pérez, B. E.; Reyes-Rodríguez, N. E.; Vega-Sánchez, V.; Martínez-Juárez, V. M.; Hernández-González, J. C. Challenges in the Detection of SARS-CoV-2: Evolution of the Lateral Flow Immunoassay as a Valuable Tool for Viral Diagnosis. Biosensors (Basel) 2022, 12 (9), 728, DOI: 10.3390/bios12090728Google ScholarThere is no corresponding record for this reference.
- 32Huang, L.; Tian, S.; Zhao, W.; Liu, K.; Ma, X.; Guo, J. Multiplexed Detection of Biomarkers in Lateral-Flow Immunoassays. Analyst 2020, 145 (8), 2828– 2840, DOI: 10.1039/C9AN02485AGoogle Scholar32https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXktFWhtLc%253D&md5=54d963c73d4a14be0378e8aadc49f263Multiplexed detection of biomarkers in lateral-flow immunoassaysHuang, Lei; Tian, Shulin; Zhao, Wenhao; Liu, Ke; Ma, Xing; Guo, JinhongAnalyst (Cambridge, United Kingdom) (2020), 145 (8), 2828-2840CODEN: ANALAO; ISSN:0003-2654. (Royal Society of Chemistry)Multiplexed detection of biomarkers, i.e., simultaneous detection of multiple biomarkers in a single assay, is a process of great advantages including enhanced diagnostic precision, improved diagnostic efficiency, reduced diagnostic cost, and alleviated pain of patients. A typical lateral-flow immunoassay (LFIA) is a widely used paper-based immunochromatog. test strip designed to detect a target biomarker through two common formats: sandwich assay and competitive assay. In order to obtain qual. or quant. results, a probe with unique optical or magnetic properties is usually employed to characterize the concn. of the target biomarker. The typical LFIA is suitable for point-of-care testing due to its simplicity, portability, cost-effectiveness, and rapid detection of a target biomarker. However, detection of a single biomarker in the typical LFIA is not favorable for high throughput anal. Therefore, multiplexed detection of biomarkers in LFIAs has been extensively studied in recent years for high throughput anal. To accomplish multiplexed detection of biomarkers in LFIAs, the most frequently used structure is a test strip with multiple test lines (TLs), where each TL can detect a specific biomarker. An alternative structure, i.e., a multi-channel structure with multiple test strips, where each test strip has one TL for detecting a specific biomarker, is employed for multiplexed detection of biomarkers. Sometimes, a single test strip with only one TL contg. different receptors, where each detection receptor corresponds to a specific biomarker, is another structure applied for multiplexed detection of biomarkers. This paper reviews three common structures for multiplexed detection of biomarkers in LFIAs, i.e., a test strip with multiple TLs, a multi-channel structure with multiple test strips, and a test strip with a single TL. Based on the three common structures, different signal detection strategies that include colorimetric detection, fluorescence detection, surface-enhanced Raman scattering detection, and magnetic detection, along with performance and perspectives are discussed.
- 33Di Nardo, F.; Chiarello, M.; Cavalera, S.; Baggiani, C.; Anfossi Ten, L. Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives. Sensors 2021, 21 (15), 5185, DOI: 10.3390/s21155185Google Scholar33https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXitl2ns7bI&md5=ea8a20e39ce32c07037b9543e2c83b60Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future PerspectivesDi Nardo, Fabio; Chiarello, Matteo; Cavalera, Simone; Baggiani, Claudio; Anfossi, LauraSensors (2021), 21 (15), 5185CODEN: SENSC9; ISSN:1424-8220. (MDPI AG)A review. The Lateral Flow Immunoassay (LFIA) is by far one of the most successful anal. platforms to perform the on-site detection of target substances. LFIA can be considered as a sort of lab-in-a-hand and, together with other point-of-need tests, has represented a paradigm shift from sample-to-lab to lab-to-sample aiming to improve decision making and turnaround time. The features of LFIAs made them a very attractive tool in clin. diagnostic where they can improve patient care by enabling more prompt diagnosis and treatment decisions. The rapidity, simplicity, relative cost-effectiveness, and the possibility to be used by nonskilled personnel contributed to the wide acceptance of LFIAs. As a consequence, from the detection of mols., organisms, and (bio)markers for clin. purposes, the LFIA application has been rapidly extended to other fields, including food and feed safety, veterinary medicine, environmental control, and many others. This review aims to provide readers with a 10-years overview of applications, outlining the trends for the main application fields and the relative compounded annual growth rates. Moreover, future perspectives and challenges are discussed.
- 34Khlebtsov, B.; Khlebtsov, N. Surface-Enhanced Raman Scattering-Based Lateral-Flow Immunoassay. Nanomaterials 2020, 10 (11), 2228, DOI: 10.3390/nano10112228Google Scholar34https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXpvFWjtQ%253D%253D&md5=ad91e149a34d0b6bbacebb724493e2fdSurface-enhanced Raman scattering-based lateral-flow immunoassayKhlebtsov, Boris; Khlebtsov, NikolaiNanomaterials (2020), 10 (11), 2228CODEN: NANOKO; ISSN:2079-4991. (MDPI AG)A review. Lateral flow immunoassays (LFIAs) have been developed and used in a wide range of applications, in point-of-care disease diagnoses, environmental safety, and food control. However, in its classical version, it has low sensitivity and can only perform semiquant. detection, based on colorimetric signals. Over the past decade, surface-enhanced Raman scattering (SERS) tags have been developed in order to decrease the detection limit and enable the quant. anal. of analytes. Of note, these tags needed new readout systems and signal processing algorithms, while the LFIA design remained unchanged. This review highlights SERS strategies of signal enhancement for LFIAs. The types of labels used, the possible gain in sensitivity from their use, methods of reading and processing the signal, and the prospects for use are discussed.
- 35Yadav, S.; Sadique Mohd, A.; Ranjan, P.; Kumar, N.; Singhal, A.; Srivastava, A. K.; Khan, R. SERS Based Lateral Flow Immunoassay for Point-of-Care Detection of SARS-CoV-2 in Clinical Samples. ACS Appl. Bio Mater. 2021, 4 (4), 2974– 2995, DOI: 10.1021/acsabm.1c00102Google Scholar35https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXmsFWhsbs%253D&md5=9052ed9f82af0653b617a56a18ae99d7SERS Based Lateral Flow Immunoassay for Point-of-Care Detection of SARS-CoV-2 in Clinical SamplesYadav, Shalu; Sadique, Mohd. Abubakar; Ranjan, Pushpesh; Kumar, Neeraj; Singhal, Ayushi; Srivastava, Avanish K.; Khan, RajuACS Applied Bio Materials (2021), 4 (4), 2974-2995CODEN: AABMCB; ISSN:2576-6422. (American Chemical Society)A review. The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role in combating the outbreak. Although conventional methods such as PCR, RT-PCR, and ELISA, etc., offer a gold-std. soln. to manage the pandemic, they cannot be implemented as a point-of-care (POC) testing arrangement. Moreover, surface-enhanced Raman spectroscopy (SERS) having a high enhancement factor provides quant. results with high specificity, sensitivity, and multiplex detection ability but lacks in POC setup. In contrast, POC devices such as lateral flow immunoassay (LFIA) offer rapid, simple-to-use, cost-effective, reliable platform. However, LFIA has limitations in quant. and sensitive analyses of SARS-CoV-2 detection. To resolve these concerns, we discuss a unique modality that is an integration of SERS with LFIA for quant. analyses of SARS-CoV-2. The miniaturization ability of SERS-based devices makes them promising in biosensor application and has the potential to make a better alternative of conventional diagnostic methods. This review also demonstrates the com. available and FDA/ICMR approved LFIA kits for on-site diagnosis of SARS-CoV-2.
- 36Langer, J.; Jimenez de Aberasturi, D.; Aizpurua, J.; Alvarez-Puebla, R. A.; Auguié, B.; Baumberg, J. J.; Bazan, G. C.; Bell, S. E. J.; Boisen, A.; Brolo, A. G.; Choo, J.; Cialla-May, D.; Deckert, V.; Fabris, L.; Faulds, K.; García de Abajo, F. J.; Goodacre, R.; Graham, D.; Haes, A. J.; Haynes, C. L.; Huck, C.; Itoh, T.; Käll, M.; Kneipp, J.; Kotov, N. A.; Kuang, H.; Le Ru, E. C.; Lee, H. K.; Li, J.-F.; Ling, X. Y.; Maier, S. A.; Mayerhöfer, T.; Moskovits, M.; Murakoshi, K.; Nam, J.-M.; Nie, S.; Ozaki, Y.; Pastoriza-Santos, I.; Perez-Juste, J.; Popp, J.; Pucci, A.; Reich, S.; Ren, B.; Schatz, G. C.; Shegai, T.; Schlücker, S.; Tay, L.-L.; Thomas, K. G.; Tian, Z.-Q.; Van Duyne, R. P.; Vo-Dinh, T.; Wang, Y.; Willets, K. A.; Xu, C.; Xu, H.; Xu, Y.; Yamamoto, Y. S.; Zhao, B.; Liz-Marzán, L. M. Present and Future of Surface-Enhanced Raman Scattering. ACS Nano 2020, 14 (1), 28– 117, DOI: 10.1021/acsnano.9b04224Google Scholar36https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1MXhs12jt7%252FN&md5=b9a563416413785a9548d12fcaa5d21aPresent and Future of Surface-Enhanced Raman ScatteringLanger, Judith; Jimenez de Aberasturi, Dorleta; Aizpurua, Javier; Alvarez-Puebla, Ramon A.; Auguie, Baptiste; Baumberg, Jeremy J.; Bazan, Guillermo C.; Bell, Steven E. J.; Boisen, Anja; Brolo, Alexandre G.; Choo, Jaebum; Cialla-May, Dana; Deckert, Volker; Fabris, Laura; Faulds, Karen; Garcia de Abajo, F. Javier; Goodacre, Royston; Graham, Duncan; Haes, Amanda J.; Haynes, Christy L.; Huck, Christian; Itoh, Tamitake; Kall, Mikael; Kneipp, Janina; Kotov, Nicholas A.; Kuang, Hua; Le Ru, Eric C.; Lee, Hiang Kwee; Li, Jian-Feng; Ling, Xing Yi; Maier, Stefan A.; Mayerhofer, Thomas; Moskovits, Martin; Murakoshi, Kei; Nam, Jwa-Min; Nie, Shuming; Ozaki, Yukihiro; Pastoriza-Santos, Isabel; Perez-Juste, Jorge; Popp, Juergen; Pucci, Annemarie; Reich, Stephanie; Ren, Bin; Schatz, George C.; Shegai, Timur; Schlucker, Sebastian; Tay, Li-Lin; Thomas, K. George; Tian, Zhong-Qun; Van Duyne, Richard P.; Vo-Dinh, Tuan; Wang, Yue; Willets, Katherine A.; Xu, Chuanlai; Xu, Hongxing; Xu, Yikai; Yamamoto, Yuko S.; Zhao, Bing; Liz-Marzan, Luis M.ACS Nano (2020), 14 (1), 28-117CODEN: ANCAC3; ISSN:1936-0851. (American Chemical Society)A review. The discovery of the enhancement of Raman scattering by mols. adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and anal. techniques. Significant exptl. and theor. effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technol., but addnl. efforts are still needed before it can be routinely used anal. and in com. products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technol. development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the prepn. of this article.
- 37Ali, A.; Nettey-Oppong, E. E.; Effah, E.; Yu, C. Y.; Muhammad, R.; Soomro, T. A.; Byun, K. M.; Choi, S. H. Miniaturized Raman Instruments for SERS-Based Point-of-Care Testing on Respiratory Viruses. Biosensors (Basel) 2022, 12 (8), 590, DOI: 10.3390/bios12080590Google ScholarThere is no corresponding record for this reference.
- 38Tran, V.; Walkenfort, B.; König, M.; Salehi, M.; Schlücker, S. Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical Chemistry. Angew. Chem., Int. Ed. 2019, 58 (2), 442– 446, DOI: 10.1002/anie.201810917Google Scholar38https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1cXitFeisbvM&md5=48768fea253a50a2331556d0f1294322Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical ChemistryTran, Vi; Walkenfort, Bernd; Koenig, Matthias; Salehi, Mohammad; Schluecker, SebastianAngewandte Chemie, International Edition (2019), 58 (2), 442-446CODEN: ACIEF5; ISSN:1433-7851. (Wiley-VCH Verlag GmbH & Co. KGaA)The design of a portable Raman/SERS-LFA reader with line illumination using a custom-made fiber optic probe for rapid, quant., and ultrasensitive point-of-care testing (POCT) is presented. The pregnancy hormone human chorionic gonadotropin (hCG) is detectable in clin. samples within only 2-5 s down to approx. 1.6 mIU mL-1. This acquisition time is several orders of magnitude shorter than those of existing approaches requiring expensive Raman instrumentation, and the method is 15-times more sensitive than a com. available lateral flow assay (LFA) as the gold std. The SERS-LFA technol. paves the way for affordable, quant., and ultrasensitive POCT with multiplexing potential in real-world applications, ranging from clin. chem. to food and environmental anal. as well as drug and biowarfare agent testing.
- 39Sloan-Dennison, S.; O’Connor, E.; Dear, J. W.; Graham, D.; Faulds, K. Towards Quantitative Point of Care Detection Using SERS Lateral Flow Immunoassays. Anal Bioanal Chem. 2022, 414 (16), 4541– 4549, DOI: 10.1007/s00216-022-03933-8Google Scholar39https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38Xislymtb4%253D&md5=30fef49c6cbe8b741032a282cc29e9f6Towards quantitative point of care detection using SERS lateral flow immunoassaysSloan-Dennison, Sian; O'Connor, Emma; Dear, James W.; Graham, Duncan; Faulds, KarenAnalytical and Bioanalytical Chemistry (2022), 414 (16), 4541-4549CODEN: ABCNBP; ISSN:1618-2642. (Springer)A review. The rapid detection of biomols. in a point of care (POC) setting is very important for diagnostic purposes. A platform which can provide this, while still being low cost and simple to use, is paper-based lateral flow immunoassays (LFIA). LFIA combine immunol. and chromatog. to detect a target by forming an immunocomplex with a label which traps them in a test zone. Qual. anal. can be performed using the naked eye while quant. anal. takes place by measuring the optical signal provided by the label at the test zone. There are numerous detection methods available; however, many suffer from low sensitivity and lack of multiplexing capabilities or are poor at providing POC quant. anal. An attractive method to overcome this is to use nanoparticles coated in Raman reporters as the labeled species and to analyze test zones using surface-enhanced Raman scattering (SERS). Due to the wide variety of metal nanoparticles, Raman reporter and laser excitations that are available, SERS-based LFIA have been adapted to identify and quantify multiple targets at once. Large Raman microscopes combined with long mapping times have limited the platform to the lab; however, by transferring the anal. to portable Raman instruments, rapid and quant. measurements can be taken at the POC without any loss in sensitivity. Portable or handheld SERS-LFIA platforms can therefore be used anywhere, from modern clinics to remote and resource-poor settings. This review will present an overview of SERS-based LFIA platforms and the major recent advancements in multiplexing and portable and handheld detection with an outlook on the future of the platform.
- 40Fu, X.; Cheng, Z.; Yu, J.; Choo, P.; Chen, L.; Choo, J. A SERS-Based Lateral Flow Assay Biosensor for Highly Sensitive Detection of HIV-1 DNA. Biosens Bioelectron 2016, 78, 530– 537, DOI: 10.1016/j.bios.2015.11.099Google Scholar40https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2MXhvFOmurfO&md5=3fba9a9b74e9c8232dea87776e247a68A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNAFu, Xiuli; Cheng, Ziyi; Yu, Jimin; Choo, Priscilla; Chen, Lingxin; Choo, JaebumBiosensors & Bioelectronics (2016), 78 (), 530-537CODEN: BBIOE4; ISSN:0956-5663. (Elsevier B.V.)User-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quant. anal. because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems assocd. with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quant. anal. of a specific biomarker in the low concn. range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quant. analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quant. detect a broad range of genetic diseases with high sensitivity.
- 41Hwang, J.; Lee, S.; Choo, J. Application of a SERS-Based Lateral Flow Immunoassay Strip for the Rapid and Sensitive Detection of Staphylococcal Enterotoxin B. Nanoscale 2016, 8 (22), 11418– 11425, DOI: 10.1039/C5NR07243CGoogle Scholar41https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC28Xlt1GgtQ%253D%253D&md5=1ac172fc6d6f779ae283bfb54e4ba64fApplication of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin BHwang, Joonki; Lee, Sangyeop; Choo, JaebumNanoscale (2016), 8 (22), 11418-11425CODEN: NANOHL; ISSN:2040-3372. (Royal Society of Chemistry)A novel surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFA) biosensor was developed to resolve problems assocd. with conventional LFA strips (e.g., limits in quant. anal. and low sensitivity). In our SERS-based biosensor, Raman reporter-labeled hollow gold nanospheres (HGNs) were used as SERS detection probes instead of gold nanoparticles. With the proposed SERS-based LFA strip, the presence of a target antigen can be identified through a color change in the test zone. Furthermore, highly sensitive quant. evaluation is possible by measuring SERS signals from the test zone. To verify the feasibility of the SERS-based LFA strip platform, an immunoassay of staphylococcal enterotoxin B (SEB) was performed as a model reaction. The limit of detection (LOD) for SEB, as detd. with the SERS-based LFA strip, was estd. to be 0.001 ng mL-1. This value is approx. three orders of magnitude more sensitive than that achieved with the corresponding ELISA-based method. The proposed SERS-based LFA strip sensor shows significant potential for the rapid and sensitive detection of target markers in a simplified manner.
- 42Tran, V.; Walkenfort, B.; Kçnig, M.; Salehi, M.; Schlücker, S. Point-of-Care Testing Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical Chemistry. Angew. Chem. Int. Ed 2019, 58, 442– 446, DOI: 10.1002/ange.201810917Google Scholar42https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1cXitFeisbvM&md5=48768fea253a50a2331556d0f1294322Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical ChemistryTran, Vi; Walkenfort, Bernd; Koenig, Matthias; Salehi, Mohammad; Schluecker, SebastianAngewandte Chemie, International Edition (2019), 58 (2), 442-446CODEN: ACIEF5; ISSN:1433-7851. (Wiley-VCH Verlag GmbH & Co. KGaA)The design of a portable Raman/SERS-LFA reader with line illumination using a custom-made fiber optic probe for rapid, quant., and ultrasensitive point-of-care testing (POCT) is presented. The pregnancy hormone human chorionic gonadotropin (hCG) is detectable in clin. samples within only 2-5 s down to approx. 1.6 mIU mL-1. This acquisition time is several orders of magnitude shorter than those of existing approaches requiring expensive Raman instrumentation, and the method is 15-times more sensitive than a com. available lateral flow assay (LFA) as the gold std. The SERS-LFA technol. paves the way for affordable, quant., and ultrasensitive POCT with multiplexing potential in real-world applications, ranging from clin. chem. to food and environmental anal. as well as drug and biowarfare agent testing.
- 43Wang, Y.; Yan, B.; Chen, L. SERS Tags: Novel Optical Nanoprobes for Bioanalysis. Chem. Rev. 2013, 113 (3), 1391– 1428, DOI: 10.1021/cr300120gGoogle Scholar43https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3sXitFWh&md5=b5a595679e1b9f09774b6e8dbbd5c345SERS Tags: Novel Optical Nanoprobes for BioanalysisWang, Yunqing; Yan, Bing; Chen, LingxinChemical Reviews (Washington, DC, United States) (2013), 113 (3), 1391-1428CODEN: CHREAY; ISSN:0009-2665. (American Chemical Society)A review on most recent advances in the use of surface enhanced Raman scattering tags.
- 44Altafini, A.; Roncada, P.; Guerrini, A.; Sonfack, G. M.; Accurso, D.; Caprai, E. Development of Histamine in Fresh and Canned Tuna Steaks Stored under Different Experimental Temperature Conditions. Foods 2022, 11 (24), 4034, DOI: 10.3390/foods11244034Google Scholar44https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhvVWrtrg%253D&md5=0e666f6f3875c0fbfe98552439d7bb10Development of Histamine in Fresh and Canned Tuna Steaks Stored under Different Experimental Temperature ConditionsAltafini, Alberto; Roncada, Paola; Guerrini, Alessandro; Sonfack, Gaetan Minkoumba; Accurso, Damiano; Caprai, ElisabettaFoods (2022), 11 (24), 4034CODEN: FOODBV; ISSN:2304-8158. (MDPI AG)Among biogenic amines, histamine is most frequently involved in foodborne intoxication. To evaluate histamine formation in tuna, several storage conditions were reproduced. An LC-MS/MS method was used for anal. detns. Fresh tuna samples (not contaminated and grafted with tuna muscle naturally incurred with histamine at 6000 mg/kg) were stored at 4, 12, and 20 °C, and daily samples were collected for 6 days. The development of histamine was obsd. only in grafted tuna samples. At 4 °C, histamine formation progressed from 12.8 mg/kg (day 1) up to 68.2 mg/kg (day 6). At 12 °C, higher concns. developed (23.9 mg/kg on day 1 up to 2721.3 mg/kg on day 6) relative to 20 °C (from 12.0 to 1681.0 mg/kg). It was found that at 4 °C, if grafted tuna was submerged in oil, histamine formation progressed more slowly. In a naturally contaminated sample, it was obsd. that the histamine distribution was uniform, while the normal cooking process did not affect the histamine level. Furthermore, it was found that the use of histamine-contaminated equipment for food handling may result in histamine formation in food. These results confirm the importance of implementing good hygiene practices and respecting the cold chain.
- 45Dwidar, M.; Yokobayashi, Y. Development of a Histamine Aptasensor for Food Safety Monitoring. Sci. Rep 2019, 9 (1), 16659, DOI: 10.1038/s41598-019-52876-1Google Scholar45https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB3MjoslymtQ%253D%253D&md5=d1152075c9d0aa76692fc36d84ad7cb2Development of a histamine aptasensor for food safety monitoringDwidar Mohammed; Yokobayashi Yohei; Dwidar MohammedScientific reports (2019), 9 (1), 16659 ISSN:.Histamine produced by bacteria through decarboxylation of histidine in spoiled foods such as fish is known to cause food poisoning. Therefore, accurate and facile measurement of histamine is of practical importance. Using the recently discovered RNA aptamer that specifically recognizes histamine (A1-949 aptamer), we developed an aptasensor based on the structure-switching mechanism. Specifically, the aptamer A1-949 was fluorescently labeled at the 5' end and hybridized with a short quencher DNA strand that is partially complementary to the aptamer. The quencher strand was modified with a fluorescence quencher at its 3' terminus. Displacement of the quencher strand upon histamine binding results in an increased fluorescence. After optimizing the assay condition, the enantiomeric version of the aptasensor (L-RNA and L-DNA) was synthesized which could detect the achiral analyte with identical sensitivity and improved biochemical stability. The aptasensor performance was validated by measuring fish samples spiked with known concentrations of histamine. Finally, histamine content in spoiled fish samples was measured, and the results were compared with the measurements using a commercial enzymatic assay kit.
- 46Shimoji, K.; Isono, E.; Bakke, M. Modified Enzymatic Assays for the Determination of Histamine in Fermented Foods. J. Food Prot 2020, 83 (8), 1430– 1437, DOI: 10.4315/JFP-20-082Google Scholar46https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXitF2qtrrN&md5=3adb30d0aa3479ac716e54f5e68c31ddModified enzymatic assays for the determination of histamine in fermented foodsShimoji, Kazuhiko; Isono, Erika; Bakke, MikioJournal of Food Protection (2020), 83 (8), 1430-1437CODEN: JFPRDR; ISSN:0362-028X. (International Association for Food Protection)Histamine is a biogenic amine, produced in spoiled fish and some fermented products, which causes a foodborne disease similar to an allergic reaction. Because regulatory levels on histamine in food have been set by many countries or organizations, a quick and accurate anal. of histamine is of great interest. An enzymic histamine detn. method on the basis of a colorimetric assay has been used to detect histamine for raw and canned tuna due to its simplicity and rapidity. However, note that some compds. in fermented foods interfere with assay results. In this study, the pretreatments and conditions of the assay for fermented foods were evaluated. Lowering the reaction temp. from 37 to 23°C was considerably effective in reducing the interference. As a result, histamine in salami and sauerkraut (≥5 to 10 mg/kg) could be detd. with a 25-fold diln., as in the manufacturer's instructions. Histamine in soy sauce (≥10 to 20 mg/L) could also be detd. with a 100-fold diln. Removing fat and protein in cheese samples by using perchloric acid with a resultant 25-fold diln. and removing polyphenol with polyvinylpolypyrrolidone for red wine with a fivefold diln. were feasible; the limits of quantification were 5 mg/kg and 1 mg/L, resp. Good recovery rates, precision repeatability, and correlations with a high-performance liq. chromatog. method were confirmed. These protocols are expected to be applicable for histamine detn. in various foods and useful for preventing histamine food poisoning.
- 47Klueber, J.; Schrama, D.; Rodrigues, P.; Dickel, H.; Kuehn, A. Fish Allergy Management: From Component-Resolved Diagnosis to Unmet Diagnostic Needs. Curr. Treat Options Allergy 2019, 6 (4), 322– 337, DOI: 10.1007/s40521-019-00235-wGoogle ScholarThere is no corresponding record for this reference.
- 48Visciano, P.; Schirone, M.; Tofalo, R.; Suzzi, G. Histamine Poisoning and Control Measures in Fish and Fishery Products. Front Microbiol 2014, 5, 5, DOI: 10.3389/fmicb.2014.00500Google ScholarThere is no corresponding record for this reference.
- 49Kobayashi, Y.; Yang, T.; Yu, C.-T.; Ume, C.; Kubota, H.; Shimakura, K.; Shiomi, K.; Hamada-Sato, N. Quantification of Major Allergen Parvalbumin in 22 Species of Fish by SDS–PAGE. Food Chem. 2016, 194, 345– 353, DOI: 10.1016/j.foodchem.2015.08.037Google Scholar49https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2MXhtlentLvI&md5=8baeeb501168fc1d49dfedfa5a3c85dbQuantification of major allergen parvalbumin in 22 species of fish by SDS-PAGEKobayashi, Yukihiro; Yang, Tao; Yu, Cheng-Tao; Ume, Chiaki; Kubota, Hiroyuki; Shimakura, Kuniyoshi; Shiomi, Kazuo; Hamada-Sato, NaokoFood Chemistry (2016), 194 (), 345-353CODEN: FOCHDJ; ISSN:0308-8146. (Elsevier Ltd.)Fish is an important causative material of food allergy. Although the allergenicity of fish is considered to correlate with the content of parvalbumin, the major fish allergen, available information about the parvalbumin content in fish is limited. In this study, a simple and reliable quantification method for fish parvalbumin by SDS-PAGE was first established. Application of the SDS-PAGE method to 22 species of fish revealed a marked variation in parvalbumin content among fish. Furthermore, the parvalbumin content was found to be higher in dorsal white muscle than in ventral white muscle, in rostral part of white muscle than in caudal part of white muscle and in white muscle than in dark muscle. IgE reactivity of fish was roughly proportional to parvalbumin content. Interestingly, large-sized migratory fish, such as salmon, swordfish and tuna, were commonly very low in both parvalbumin content and IgE reactivity.
- 50Lim, D. L.-C.; Neo, K. H.; Goh, D. L.-M.; Shek, L. P.-C.; Lee, B. W. Missing Parvalbumin: Implications in Diagnostic Testing for Tuna Allergy. Journal of Allergy and Clinical Immunology 2005, 115 (4), 874– 875, DOI: 10.1016/j.jaci.2004.12.1117Google Scholar50https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BD2MXivV2is7Y%253D&md5=95faf2dd2dc0ed19d3c92e2d9ab03c1dMissing parvalbumin: Implications in diagnostic testing for tuna allergyLim, Dawn Li-Chern; Neo, Keng Hwee; Goh, Denise Li-Meng; Shek, Lynette Pei-Chi; Lee, Bee WahJournal of Allergy and Clinical Immunology (2005), 115 (4), 874-875CODEN: JACIBY; ISSN:0091-6749. (Elsevier Inc.)This study aims at establishing the reasons for inconsistent findings regarding the presence and absence of parvalbumin, a 12-kd protein, in tuna. A fresh whole tuna, Thunnus tonggol (Bleeker) of about 400-mm fork length (FL) was skinned and cut transversely into sections along the length, beginning about 20 mm posterior to the edge of the operculum, with each section measuring about 25 mm in thickness. Parvalbumin was found in the white muscle of tuna and was absent in the red. It was also unequally distributed, with ventral white muscle contg. slightly more parvalbumin than the dorsal white muscle. The amt. of parvalbumin decreased from the rostral region to the caudal region. The findings provide an explanation for the inconsistencies in the earlier reports of parvalbumin in tuna. It is likely that different parts of the fish were sampled, and those reporting absence of parvalbumin were likely to have used a substantial portion of the red muscle. This observation is likely to be unique to fish with large portions of red muscle in sections of its torso. Although white meat of tuna is favored when eaten raw, both white and red meat of the fish are consumed in the cooked form. Therefore, these findings have significant implications when prepg. tuna fish diagnostic allergen exts. from raw material.
- 51Schrama, D.; Raposo de Magalhães, C.; Cerqueira, M.; Carrilho, R.; Revets, D.; Kuehn, A.; Engrola, S.; Rodrigues, P. M. Fish Processing and Digestion Affect Parvalbumins Detectability in Gilthead Seabream and European Seabass. Animals 2022, 12 (21), 3022, DOI: 10.3390/ani12213022Google ScholarThere is no corresponding record for this reference.
- 52Aksun Tümerkan, E. T. Detection of Parvalbumin Fish Allergen in Canned Tuna by Real-Time PCR Driven by Tuna Species and Can-Filling Medium. Molecules 2022, 27 (17), 5674, DOI: 10.3390/molecules27175674Google ScholarThere is no corresponding record for this reference.
- 53Taki, A. C.; Ruethers, T.; Nugraha, R.; Karnaneedi, S.; Williamson, N. A.; Nie, S.; Leeming, M. G.; Mehr, S. S.; Campbell, D. E.; Lopata, A. L. Thermostable Allergens in Canned Fish: Evaluating Risks for Fish Allergy. Allergy 2023, 78, 3221– 3234, DOI: 10.1111/all.15864Google Scholar53https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhvVGlt77M&md5=fcaf2186cc0c16f70a7c64a0d3eb92f3Thermostable allergens in canned fish: Evaluating risks for fish allergyTaki, Aya C.; Ruethers, Thimo; Nugraha, Roni; Karnaneedi, Shaymaviswanathan; Williamson, Nicholas A.; Nie, Shuai; Leeming, Michael G.; Mehr, Sam S.; Campbell, Dianne E.; Lopata, Andreas L.Allergy (Oxford, United Kingdom) (2023), 78 (12), 3221-3234CODEN: LLRGDY; ISSN:0105-4538. (Wiley-Blackwell)Major fish allergens, including parvalbumin (PV), are heat stable and can withstand extensive cooking processes. Thus, the management of fish allergy generally relies on complete avoidance. Fish-allergic patients may be advised to consume canned fish, as some fish-allergic individuals have reported tolerance to canned fish. However, the safety of consuming canned fish has not been evaluated with comprehensive immunol. and mol. anal. of canned fish products. We characterized the in vitro immunoreactivity of serum obtained from fish-allergic subjects to canned fish. Seventeen canned fish products (salmon n = 8; tuna n = 7; sardine n = 2) were assessed for the content and integrity of PV using allergen-specific antibodies. Subsequently, the sIgE binding of five selected products was evaluated for individual fish-allergic patients (n = 53). Finally, sIgE-binding proteins were identified by mass spectrometry. The canned fish showed a markedly reduced PV content and binding to PV-specific antibodies compared with conventionally cooked fish. However, PV and other heat-stable fish allergens, including tropomyosin and collagen, still maintained their sIgE-binding capacity. Of 53 patients, 66% showed sIgE binding to canned fish proteins. The canned sardine contained proteins bound to sIgE from 51% of patients, followed by canned salmon (43%-45%) and tuna (8%-17%). PV was the major allergen in canned salmon and sardine. Tropomyosin and/or collagen also showed sIgE binding. We showed that canned fish products may not be safe for all fish-allergic patients. Canned fish products should only be considered into the diet of individuals with fish allergy, after detailed evaluation which may include in vitro diagnostics to various heat-stable fish allergens and food challenge conducted in suitable environments.
- 54Blanco-Covián, L.; Montes-García, V.; Girard, A.; Fernández-Abedul, M. T.; Pérez-Juste, J.; Pastoriza-Santos, I.; Faulds, K.; Graham, D.; Blanco-López, M. C. Au@Ag SERRS Tags Coupled to a Lateral Flow Immunoassay for the Sensitive Detection of Pneumolysin. Nanoscale 2017, 9 (5), 2051– 2058, DOI: 10.1039/C6NR08432JGoogle Scholar54https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2sXhtFCisQ%253D%253D&md5=b59479379103d80b4279fca476a3df7fAu@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysinBlanco-Covian, Lucia; Montes-Garcia, Veronica; Girard, Alexandre; Fernandez-Abedul, M. Teresa; Perez-Juste, Jorge; Pastoriza-Santos, Isabel; Faulds, Karen; Graham, Duncan; Blanco-Lopez, M. CarmenNanoscale (2017), 9 (5), 2051-2058CODEN: NANOHL; ISSN:2040-3372. (Royal Society of Chemistry)Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labeled probe. The combination of Au@Ag core-shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL-1. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.
- 55Fernández-Lodeiro, C.; Fernández-Lodeiro, J.; Carbó-Argibay, E.; Lodeiro, C.; Pérez-Juste, J.; Pastoriza-Santos, I. The Versatility of Fe(II) in the Synthesis of Uniform Citrate-Stabilized Plasmonic Nanoparticles with Tunable Size at Room Temperature. Nano Res. 2020, 13 (9), 2351– 2355, DOI: 10.1007/s12274-020-2854-1Google Scholar55https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXhsVGjtL%252FE&md5=9c57363f2ca6a5095a70a13267ef549eThe versatility of Fe (II) in the synthesis of uniform citrate-stabilized plasmonic nanoparticles with tunable size at room temperatureFernandez-Lodeiro, Carlos; Fernandez-Lodeiro, Javier; Carbo-Argibay, Enrique; Lodeiro, Carlos; Perez-Juste, Jorge; Pastoriza-Santos, IsabelNano Research (2020), 13 (9), 2351-2355CODEN: NRAEB5; ISSN:1998-0000. (Springer GmbH)A highly versatile seed-mediated approach for the synthesis of citrate-stabilized gold, silver and palladium nanoparticles (NPs) with size control is reported. The use of iron(II) as a reducing agent enables the fabrication of monodisperse NPs in a wide range of sizes (from 15 nm to at least 120 nm (90 nm for Pd)) at room temp. The citrate as capping ligand on the NPs surface facilitates its further surface modification with proteins and thiolated mols.
- 56Rodbard, D. Statistical Quality Control and Routine Data Processing for Radioimmunoassays and Immunoradiometric Assays. Clin Chem. 1974, 20 (10), 1255– 1270, DOI: 10.1093/clinchem/20.10.1255Google Scholar56https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADyaE2MXisFGmuw%253D%253D&md5=993d52b7426cda4df8232c848f481ef2Statistical quality control and routine data processing for radioimmunoassays and immunoradiometric assaysRodbard, DavidClinical Chemistry (Washington, DC, United States) (1974), 20 (10), 1255-70CODEN: CLCHAU; ISSN:0009-9147.A review with 43 refs.
- 57Moyano, A.; Salvador, M.; Martínez-García, J. C.; Socoliuc, V.; Vékás, L.; Peddis, D.; Alvarez, M. A.; Fernández, M.; Rivas, M.; Blanco-López, M. C. Magnetic Immunochromatographic Test for Histamine Detection in Wine. Anal Bioanal Chem. 2019, 411 (25), 6615– 6624, DOI: 10.1007/s00216-019-02031-6Google Scholar57https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1MXhsVGqtLvJ&md5=aa80973a002119243c41e8b9a9b2b31cMagnetic immunochromatographic test for histamine detection in wineMoyano, Amanda; Salvador, Maria; Martinez-Garcia, Jose C.; Socoliuc, Vlad; Vekas, Ladislau; Peddis, Davide; Alvarez, Miguel A.; Fernandez, Maria; Rivas, Montserrat; Blanco-Lopez, M. CarmenAnalytical and Bioanalytical Chemistry (2019), 411 (25), 6615-6624CODEN: ABCNBP; ISSN:1618-2642. (Springer)Histamine, a biogenic amine, is abundant in fermented foods and beverages, notably wine. A high intake of this monoamine may produce adverse reactions in humans, which may be severe in individuals with a reduced capacity to catabolise extrinsic histamine. Thus, control of histamine concn. during wine prodn. and before distribution is advisable. Simple, rapid, point-of-use bioanal. platforms are needed because traditional methods for the detection and quantification of histamine are expensive and time-consuming. This work applies the lateral flow immunoassay technique to histamine detection. Superparamagnetic particle labels, and an inductive sensor designed to read the test line in the immunoassay, enable magnetic quantification of the mol. The system is calibrated with histamine stds. in the interval of interest for wine prodn. A com. optical strip reader is used for comparison measurements. The lateral flow system has a limit of detection of 1.2 and 1.5 mg/L for the inductive and optical readers, resp. The capability of the inductive system for histamine quantification is demonstrated for wine samples at different processing points (at the end of alc. fermn., at the end of malolactic fermn., in freshly bottled wine, and in reserve wine). The results are validated by ultra-high-performance liq. chromatog.
- 58Wang, Q.; Haughey, S. A.; Sun, Y.-M.; Eremin, S. A.; Li, Z.-F.; Liu, H.; Xu, Z.-L.; Shen, Y.-D.; Lei, H.-T. Development of a Fluorescence Polarization Immunoassay for the Detection of Melamine in Milk and Milk Powder. Anal Bioanal Chem. 2011, 399 (6), 2275– 2284, DOI: 10.1007/s00216-010-4599-2Google Scholar58https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3MXjtFKitQ%253D%253D&md5=060fde82fd0f3e07c38af90eb21c1a3bDevelopment of a fluorescence polarization immunoassay for the detection of melamine in milk and milk powderWang, Qiang; Haughey, Simon A.; Sun, Yuan-Ming; Eremin, Sergei A.; Li, Zhen-Feng; Liu, Hui; Xu, Zhen-Lin; Shen, Yu-Dong; Lei, Hong-TaoAnalytical and Bioanalytical Chemistry (2011), 399 (6), 2275-2284CODEN: ABCNBP; ISSN:1618-2642. (Springer)A fluorescence polarization immunoassay (FPIA) based on a polyclonal antibody was developed for the detn. of melamine in milk. To obtain an antibody with improved sensitivity and specificity, 6-hydrazinyl-1,3,5-triazine-2,4-diamine was coupled to bovine serum albumin and used as the immunogen for the rabbit immunization. Three fluorescein-labeled melamine tracers with different structures and spacer bridges were synthesized. The structural effect of the tracers on the assay characteristics was investigated. 6-(4,6-Diamino-1,3,5-triazin-2-ylamino)-N-(2-(3-(3',6'-dihydroxy-3-oxo-2,3-dihydrospiro[indene-1,9'-xanthene]-5-yl)thioureido)ethyl)hexanamide demonstrated better sensitivity than 5-(2-(4,6-diamino-1,3,5-triazin-2-yl)hydrazinecarbothioamido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid and 3-(4,6-diamino-1,3,5-triazin-2-ylthio)-N-(2-(3-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-yl)thioureido)ethyl)propanamide. The limit of detection (10% inhibition) of the FPIA was 9.3 ng mL-1 and the IC50 (50% inhibition) value was 164.7 ng mL-1. The antibody in the FPIA showed 21.2% cross-reactivity to the fly-killing insecticide cyromazine, but had no cross-reactivity to other natural structurally related compds. Recoveries, measured in spiked milk and milk powder samples, ranged from 79.4 to 119.0%. Milk samples fortified with melamine were analyzed by this method and confirmed by high-performance liq. chromatog.-mass spectrometry. Excellent recoveries and correlation with spiked levels were obsd., suggesting that this immunoassay could be applied to the screening of melamine residues in milk and milk powder after a simple diln. procedure.
- 59Guo, M.; Zhang, J.; Lv, J.; Ke, T.; Tian, J.; Miao, K.; Wang, Y.; Kong, D.; Ruan, H.; Luo, J.; Yang, M. Development of Broad-Specific Monoclonal Antibody-Based Immunoassays for Simultaneous Ochratoxin Screening in Medicinal and Edible Herbs. Food Control 2023, 148, 109626 DOI: 10.1016/j.foodcont.2023.109626Google Scholar59https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhsFSmtb0%253D&md5=4a3dc28cfbc2225b36be7da5e89d0dbdDevelopment of broad-specific monoclonal antibody-based immunoassays for simultaneous ochratoxin screening in medicinal and edible herbsGuo, Mengyue; Jing, zhang; Lv, Jianxin; Ke, Tongwei; Tian, Jiao; Miao, Kun; Wang, Yudan; Kong, Dandan; Ruan, Haonan; Luo, Jiaoyang; Yang, MeihuaFood Control (2023), 148 (), 109626CODEN: FOOCEV; ISSN:0956-7135. (Elsevier Ltd.)The frequent occurrence of ochratoxins in food and herbs poses a considerable threat to consumer and animal health. Here, we generated a broad-specificity monoclonal antibody against three ochratoxins. Concns. for 50% inhibition (IC50) for OTA, OTB, and OTC were 0.37, 0.23, and 2.24 ng/mL, resp. In the two matrixes, the proposed indirect competitive ELISA (ic-ELISA) exhibited a limit of detection (LOD) of 1.5μg/kg for OTA and 1.0μg/kg for OTB, resp., whereas the cut-off levels of lateral flow immunoassay (LFIA) for OTA and OTB were resp. detd. to be 1 and 0.25 ng/mL. As for OTC, the LOD of ic-ELISA and cut-off concn. of LFIA were 15.5μg/kg and 8 ng/mL for milkvetch root and 37.5μg/kg and 16 ng/mL for ginger, resp. The real sample detection results by ic-ELISA and LFIA methods were validated via LC-MS/MS anal. Therefore, the developed immunoassays are useful and effective tools for high-throughput screening and rapid on-site detn. of ochratoxins in complex matrixes.
- 60Yang, F.; Xu, L.; Dias, A. C. P.; Zhang, X. A Sensitive Sandwich ELISA Using a Modified Biotin-Streptavidin Amplified System for Histamine Detection in Fish Prawn and Crab. Food Chem. 2021, 350, 129196 DOI: 10.1016/j.foodchem.2021.129196Google Scholar60https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXktF2qsbo%253D&md5=2ce29bc2794b9bc0d98821ea81b8be26A sensitive sandwich ELISA using a modified biotin-streptavidin amplified system for histamine detection in fish, prawn and crabYang, Fanfan; Xu, Long; Dias, Alberto C. P.; Zhang, XiaoyingFood Chemistry (2021), 350 (), 129196CODEN: FOCHDJ; ISSN:0308-8146. (Elsevier Ltd.)Histamine poisoning from seafood is a significant public health and safety concern. To detect histamine sensitively and accurately, a novel competitive sandwich immunoassay using a modified biotin-streptavidin system coupling with polylysine was developed. Using this strategy, a sandwich ELISA with an IC50 value of 112.8 ng mL-1 and a broad linear range of 11.7-1500 ng mL-1 with a correlation coeff. of 0.9942 was validated. Without any sample derivatization procedure, the recovery of histamine ranged from 80.19% to 108.3% with a coeff. of variation of 1.43-11.7% in tuna, prawn and crab. The sandwich ELISA had a detection limit of 5.86 ng mL-1, which was 15-fold lower than an indirect competitive ELISA (ic-ELISA). This simple, sensitive and accurate method can be applied to detect histamine in routine seafood samples.
- 61DeBeeR, J.; Bell, J. W.; Nolte, F.; Arcieri, J.; Correa, G. Histamine Limits by Country: A Survey and Review. J. Food Prot 2021, 84 (9), 1610– 1628, DOI: 10.4315/JFP-21-129Google Scholar61https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB2c%252FjvFSktQ%253D%253D&md5=dfa80b4c23dd8f1fffd5146d88991365Histamine Limits by Country: A Survey and ReviewDeBEER John; Bell Jon W; Nolte Fred; Arcieri Julian; Correa GersonJournal of food protection (2021), 84 (9), 1610-1628 ISSN:.Histamine is a biogenic amine and a food safety hazard, and it is the only biogenic amine regulated by statute or hazard analysis and critical control point guidance. This article reviews the regulations for histamine levels in fish in countries around the world, including maximum limits or levels and sampling procedures in different fish preparations. The maximum histamine levels, sampling plans, and fish products are listed. The country-by-country regulations for maximum histamine acceptance levels in some food products vary by a factor of 8, from 50 ppm in some countries to a maximum of 400 ppm in other countries. For similar food products, the maximum histamine levels vary by a factor of 4 (from 50 ppm to 200 ppm) in, for example, fresh tuna. The country-by-country sampling plans vary widely as well, and these, too, are covered in detail.
- 62Schulz, F.; Homolka, T.; Bastús, N. G.; Puntes, V.; Weller, H.; Vossmeyer, T. Little Adjustments Significantly Improve the Turkevich Synthesis of Gold Nanoparticles. Langmuir 2014, 30 (35), 10779– 10784, DOI: 10.1021/la503209bGoogle Scholar62https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2cXhtlOntLvF&md5=50501206d9a841afa7243b2b24826428Little Adjustments Significantly Improve the Turkevich Synthesis of Gold NanoparticlesSchulz, Florian; Homolka, Torge; Bastus, Neus G.; Puntes, Victor; Weller, Horst; Vossmeyer, TobiasLangmuir (2014), 30 (35), 10779-10784CODEN: LANGD5; ISSN:0743-7463. (American Chemical Society)The classical and widely used Turkevich synthesis can be improved significantly by simple adjustments. The Au nanoparticles (AuNPs) produced with the optimized protocol have a much narrower size distribution (5-8% std. deviation), and their diams. can be reproduced with unrivaled little variation (<3%). Also, large vols. of these particles can be produced in one synthesis; the authors routinely synthesize 1000 mL of ∼3.5 nM AuNPs. The key features of the improved protocol are the control of the pH by using a citrate buffer instead of a citrate soln. as the reducing agent or stabilizer and optimized mixing of reagents. Further, the shape uniformity of the particles can be improved by addn. of 0.02 mM EDTA. While the proposed protocol is as straightforward as the original Turkevich protocol, it is more tolerant against variations in precursor concn.
- 63Carrera, M.; Cañas, B.; Gallardo, J. M. Rapid Direct Detection of the Major Fish Allergen, Parvalbumin, by Selected MS/MS Ion Monitoring Mass Spectrometry. J. Proteomics 2012, 75 (11), 3211– 3220, DOI: 10.1016/j.jprot.2012.03.030Google Scholar63https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC38XlvFymtrs%253D&md5=faeaacef2d1ccd790e5fe85c11b6732eRapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometryCarrera, Monica; Canas, Benito; Gallardo, Jose M.Journal of Proteomics (2012), 75 (11), 3211-3220CODEN: JPORFQ; ISSN:1874-3919. (Elsevier B.V.)Parvalbumins beta (β-PRVBs) are considered the major fish allergens. A new strategy for the rapid and direct detection of these allergens in any foodstuff is presented in this work. The proposed methodol. is based on the purifn. of β-PRVBs by treatment with heat, the use of accelerated in-soln. trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the direct detection of the presence of fish β-PRVBs in any food product in less than 2 h.
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Abstract
Scheme 1
Scheme 1. Schematic Depicting a Dual Colorimetric and SERS-Based Competitive LFIA for Simultaneous Detection and Quantification of Parvalbumin and HistamineaaTwo well-differentiated SERS tags conjugated with anti-β-parvalbumin (αParv) and anti-histamine (αHist) are synthesized and mixed with a canned tuna extract. Subsequently, a dual colorimetric SERS-based detection and discrimination of the antigens is performed.
Figure 1
Figure 1. SERS tag characterization. (A) Representative TEM image of spherical Au@Ag core–shell nanoparticles (Au@Ag NPs). (B) Size distribution histogram of Au@Ag NPs. (C) SERS spectra of the SERS tags encoded with MGITC (green) and RBITC (red). The green and red shadowed regions indicate 1616 and 1646 cm–1 Raman peaks of MGITC and RBITC-encoded SERS tags, respectively. (D) Normalized extinction spectra of Au@Ag NPs before (black) and after functionalization with RBITC and anti-β-parvalbumin (red line) and with MGITC and antihistamine (green line). The inset clearly shows the red shift in the plasmon band peak after the codification of Au@Ag NPs.
Figure 2
Figure 2. Nanoprobe cross-reactivity assessment. (A) Photograph of the LFIA strip with histamine (Hist), parvalbumin (Parv), and protein-G (PG) immobilized in the test (T) and control (C) lines, as indicated. SERS intensity mappings acquired at 1616 and 1646 cm–1 which are characteristic peaks of αHist-MGITC SERS or αParv-RBITC SERS tags, respectively. (B) Average SERS spectra from the 20 highest intensity points measured in each line. The green and red shadowed regions indicate 1616 and 1646 cm–1 Raman peaks of the MGITC and RBITC-encoded tags. Scale bars in (A) represent 1 mm. Scale bars in (B) represent 1 Kcts mW–1 s–1. All SERS measurements were carried out with a 532 nm laser line, 10× objective, 0.25 mW laser power, acquisition time 0.5 s, and 231 points.
Figure 3
Figure 3. (A and B) Photographs of LFIA strips after running αParv-RBITC SERS tags and αHist- MGITC SERS tags previously incubated with different concentrations of (A) histamine (from 2.5 to 5 × 10–6 mg mL–1) or (B) parvalbumin (from 0.5 to 2.5 × 10–4 mg mL–1) in PB. (C and D) Average SERS spectra acquired from the different Hist (C) and Parv (D) T lines are shown in (A) and (B), respectively. (E, F) Variation of SERS intensity at 1646 cm–1 (E) or 1616 cm–1 (F) with the concentration of parvalbumin and histamine, respectively. The red lines represent the fitting of the SERS intensity measurements to a four-parameter sigmoid equation. Standard deviations correspond to the 20 higher-intensity SERS points of each strip. All SERS measurements were carried out with a 532 nm laser line, 10× objective, 0.25, 2.31, or 12.50 mW laser power depending on the color intensity of the test lines, 1.0 s acquisition time, and 143 points.
Figure 4
Figure 4. (A) Photograph of an LFIA strip with a control (C) line and two test lines for parvalbumin (T Parv) and histamine (T Hist), as indicated, and representative SERS spectra measured in each T line of the LFIA strip with a hand-held Raman spectrometer with a 532 nm laser line, 21 mW laser power, and 1.0 s acquisition time. The scale bar represents 5 Kcts mW–1 s–1. (B, C) Optical sensor linear regression range of parvalbumin (B) and histamine (C) obtained by analyzing extracts of canned tuna.
Figure 5
Figure 5. (A) Photograph of four LFIA strips corresponding to experiments performed in the absence of histamine and parvalbumin (1), the presence of histamine and parvalbumin in excess (2), the presence of parvalbumin and no histamine (3) and the presence of histamine and no parvalbumin (4) immobilized in the test (T) line. (B) Representative SERS spectra measured with a hand-held Raman in the different T lines, as indicated. SERS measurements were performed with a 532 nm laser line, 21 mW and 1 s acquisition time. The shadowed regions indicate characteristic Raman peaks of αHist-MGITC SERS tags (1616 cm–1, green) and αParv-RBITC SERS tags (1646 cm–1, in red). The scale bar represents 0.5 Kcts mW–1 s–1. (C) SERS intensity mappings acquired at 1616 cm–1 (left) and 1646 cm–1 (right) in the different T lines from (A), as indicated, showing the presence/absence and spatial distribution of αParv-RBITC SERS tags and αHist-MGITC SERS tags, respectively. Scale bars are 1 mm. SERS mappings were carried out with a 532 nm laser line, 10× objective, 2.31 or 12.50 mW laser power depending on the color intensity of the test lines, acquisition time 1.0 s, and 231 points.
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- 1Boyd, C. E.; McNevin, A. A.; Davis, R. P. The Contribution of Fisheries and Aquaculture to the Global Protein Supply. Food Secur 2022, 14 (3), 805– 827, DOI: 10.1007/s12571-021-01246-91https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB2M7islGjug%253D%253D&md5=8fc501dd873e47c708c38a50b4cc685dThe contribution of fisheries and aquaculture to the global protein supplyBoyd Claude E; Davis Robert P; McNevin Aaron AFood security (2022), 14 (3), 805-827 ISSN:1876-4517.The contribution of aquatic animal protein to the global, animal-source protein supply and the relative importance of aquaculture to capture fisheries in supplying this protein is relevant in assessments and decisions related to the future of aquatic food production and its security. Meat of terrestrial animals, milk, and eggs resulted in 76,966 Kt crude protein compared with 13,950 Kt or 15.3% from aquatic animals in 2018.While aquaculture produced a greater tonnage of aquatic animals, capture fisheries resulted in 7,135 Kt crude protein while aquaculture yielded 6,815 Kt. Capture fisheries production has not increased in the past two decades, and aquaculture production must increase to assure the growing demand for fisheries products by a larger and more affluent population. We estimated based on status quo consumption, that aquaculture production would need to increase from 82,087 Kt in 2018 to 129,000 Kt by 2050 to meet the demand of the greater population. About two-thirds of finfish and crustacean production by aquaculture is feed-based, and feeds for these species include fishmeal and fish oil as ingredients. Aquaculture feeds require a major portion of the global supply of fishmeal and fish oil. An estimated 71.0% of fishmeal and 73.9% of fish oil are made from the catch with the rest coming from aquatic animal processing waste. The catch of small, pelagic fish from the ocean is not predicted to increase in the future. Aquaculture should reduce its fishmeal and oil use to lessen its dependency on small wild fish important to the integrity of marine food webs and food security for the poor in many coastal areas. Fishmeal and fish oil shortages for use in aquaculture feed will result in a limit on production in the future if goals to lessen their use in feeds are not met.
- 2Pettoello-Mantovani, C.; Olivieri, B. Food Safety and Public Health within the Frame of the EU Legislation. Global Pediatrics 2022, 2, 100020 DOI: 10.1016/j.gpeds.2022.100020There is no corresponding record for this reference.
- 3Nwaru, B. I.; Hickstein, L.; Panesar, S. S.; Muraro, A.; Werfel, T.; Cardona, V.; Dubois, A. E. J.; Halken, S.; Hoffmann-Sommergruber, K.; Poulsen, L. K.; Roberts, G.; Van Ree, R.; Vlieg-Boerstra, B. J.; Sheikh, A. The Epidemiology of Food Allergy in Europe: A Systematic Review and Meta-Analysis. Allergy 2014, 69 (1), 62– 75, DOI: 10.1111/all.123053https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BC2c7ksVKltw%253D%253D&md5=1080fa6e691319f4cf09fc9af71bc740The epidemiology of food allergy in Europe: a systematic review and meta-analysisNwaru B I; Hickstein L; Panesar S S; Muraro A; Werfel T; Cardona V; Dubois A E J; Halken S; Hoffmann-Sommergruber K; Poulsen L K; Roberts G; Van Ree R; Vlieg-Boerstra B J; Sheikh AAllergy (2014), 69 (1), 62-75 ISSN:.Food allergy (FA) is an important atopic disease although its precise burden is unclear. This systematic review aimed to provide recent, up-to-date data on the incidence, prevalence, time trends, and risk and prognostic factors for FA in Europe. We searched four electronic databases, covering studies published from 1 January 2000 to 30 September 2012. Two independent reviewers appraised the studies and qualified the risk of bias using the Critical Appraisal Skills Programme tool. Seventy-five eligible articles (comprising 56 primary studies) were included in a narrative synthesis, and 30 studies in a random-effects meta-analysis. Most of the studies were graded as at moderate risk of bias. The pooled lifetime and point prevalence of self-reported FA were 17.3% (95% CI: 17.0-17.6) and 5.9% (95% CI: 5.7-6.1), respectively. The point prevalence of sensitization to ≥1 food as assessed by specific IgE was 10.1% (95% CI: 9.4-10.8) and skin prick test 2.7% (95% CI: 2.4-3.0), food challenge positivity 0.9% (95% CI: 0.8-1.1). While the incidence of FA appeared stable over time, there was some evidence that the prevalence may be increasing. There were no consistent risk or prognostic factors for the development or resolution of FA identified, but sex, age, country of residence, familial atopic history, and the presence of other allergic diseases seem to be important. Food allergy is a significant clinical problem in Europe. The evidence base in this area would benefit from additional studies using standardized, rigorous methodology; data are particularly required from Eastern and Southern Europe.
- 4Kalic, T.; Radauer, C.; Lopata, A. L.; Breiteneder, H.; Hafner, C. Fish Allergy Around the World─Precise Diagnosis to Facilitate Patient Management. Frontiers in Allergy 2021, 2, 2, DOI: 10.3389/falgy.2021.732178There is no corresponding record for this reference.
- 5Ho, M. H.-K.; Wong, W. H.-S.; Chang, C. Clinical Spectrum of Food Allergies: A Comprehensive Review. Clin Rev. Allergy Immunol 2014, 46 (3), 225– 240, DOI: 10.1007/s12016-012-8339-65https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2cXntlyrsrk%253D&md5=54527a68d2ae8f0421e3fe56e7ab653dClinical Spectrum of Food Allergies: a Comprehensive ReviewHo, Marco H.-K.; Wong, Wilfred H.-S.; Chang, ChristopherClinical Reviews in Allergy & Immunology (2014), 46 (3), 225-240CODEN: CRAIF2; ISSN:1080-0549. (Springer)A review. Food allergy is defined as an adverse immune response towards food proteins or as a form of a food intolerance assocd. with a hypersensitive immune response. It should also be reproducible by a double-blind placebo-controlled food challenge. Many reported that food reactions are not allergic but are intolerances. Food allergy often presents to clinicians as a symptom complex. This review focuses on the clin. spectrum and manifestations of various forms of food allergies. According to clin. presentations and allergy testing, there are three types of food allergy: IgE mediated, mixed (IgE/Non-IgE), and non-IgE mediated (cellular, delayed type hypersensitivity). Recent advances in food allergy in early childhood have highlighted increasing recognition of a spectrum of delayed-onset non-IgE-mediated manifestation of food allergy. Common presentations of food allergy in infancy including atopic eczema, infantile colic, and gastroesophageal reflux. These clin. observations are frequently assocd. with food hypersensitivity and respond to dietary elimination. Non-IgE-mediated food allergy includes a wide range of diseases, from atopic dermatitis to food protein-induced enterocolitis and from eosinophilic esophagitis to celiac disease. The most common food allergies in children include milk, egg, soy, wheat, peanut, treenut, fish, and shellfish. Milk and egg allergies are usually outgrown, but peanut and treenut allergy tends to persist. The prevalence of food allergy in infancy is increasing and may affect up to 15-20 % of infants. The alarming rate of increase calls for a public health approach in the prevention and treatment of food allergy in children.
- 6Griesmeier, U.; Vázquez-Cortés, S.; Bublin, M.; Radauer, C.; Ma, Y.; Briza, P.; Fernaindez-Rivas, M.; Breiteneder, H. Expression Levels of Parvalbumins Determine Allergenicity of Fish Species. Allergy 2010, 65 (2), 191– 198, DOI: 10.1111/j.1398-9995.2009.02162.x6https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3cXhslOhtrY%253D&md5=2d1384f630cb90743e7a5a637a5241f4Expression levels of parvalbumins determine allergenicity of fish speciesGriesmeier, U.; Vazquez-Cortes, S.; Bublin, M.; Radauer, C.; Ma, Y.; Briza, P.; Fernandez-Rivas, M.; Breiteneder, H.Allergy (Oxford, United Kingdom) (2010), 65 (2), 191-198CODEN: LLRGDY; ISSN:0105-4538. (Wiley-Blackwell)Background: Parvalbumins are the most important fish allergens. Polysensitization to various fish species is frequently reported and linked to the cross-reactivity of their parvalbumins. Studies on cross-reactivity and its assocn. to the allergenicity of purified natural parvalbumins from different fish species are still lacking. In addn., some studies indicate that dark muscled fish such as tuna are less allergenic. Methods: Total protein exts. and purified parvalbumins from cod, whiff, and swordfish, all eaten frequently in Spain, were tested for their IgE-binding properties with 16 fish allergic patients' sera from Madrid. The extent of cross-reactivity of these parvalbumins was investigated by IgE ELISA inhibition assays. Addnl., the cDNA sequences of whiff and swordfish parvalbumins were detd. Results: Extractable amts. of parvalbumins from cod were 20 times and from whiff 30 times higher than from swordfish. Parvalbumins were recognized by 94% of the patients in exts. of cod and whiff, but only by 60% in swordfish exts. Nevertheless, a high cross-reactivity was detd. for all purified parvalbumins by IgE inhibition. The amino acid sequence identities of the three parvalbumins were in a range of 62-74%. Conclusions: The parvalbumins of cod, whiff and swordfish are highly cross-reactive. The high amino acid sequence identity among cod, whiff and swordfish parvalbumins results in the obsd. IgE cross-reactivity. The low allergenicity of swordfish is due to the low expression levels of its parvalbumin.
- 7Kuehn, A.; Swoboda, I.; Arumugam, K.; Hilger, C.; Hentges, F. Fish Allergens at a Glance: Variable Allergenicity of Parvalbumins, the Major Fish Allergens. Front Immunol 2014, 5, 5, DOI: 10.3389/fimmu.2014.00179There is no corresponding record for this reference.
- 8Zhernov, Y. V.; Simanduyev, M. Y.; Zaostrovtseva, O. K.; Semeniako, E. E.; Kolykhalova, K. I.; Fadeeva, I. A.; Kashutina, M. I.; Vysochanskaya, S. O.; Belova, E. V.; Shcherbakov, D. V.; Sukhov, V. A.; Sidorova, E. A.; Mitrokhin, O. V. Molecular Mechanisms of Scombroid Food Poisoning. Int. J. Mol. Sci. 2023, 24 (1), 809, DOI: 10.3390/ijms240108098https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhtVSlt7o%253D&md5=1ab09c59d97cb920ac3405dc291bf381Molecular Mechanisms of Scombroid Food PoisoningZhernov, Yury V.; Simanduyev, Mark Y.; Zaostrovtseva, Olga K.; Semeniako, Ekaterina E.; Kolykhalova, Kseniia I.; Fadeeva, Inna A.; Kashutina, Maria I.; Vysochanskaya, Sonya O.; Belova, Elena V.; Shcherbakov, Denis V.; Sukhov, Vitaly A.; Sidorova, Ekaterina A.; Mitrokhin, Oleg V.International Journal of Molecular Sciences (2023), 24 (1), 809CODEN: IJMCFK; ISSN:1422-0067. (MDPI AG)A review. Scombroid food poisoning (SFP) is a foodborne disease that develops after consumption of fresh fish and, rarely, seafood that has fine organoleptic characteristics but contains a large amt. of exogenous histamine. SFP, like other food pseudo-allergic reactions (FPA), is a disorder that is clin. identical to allergic reactions type I, but there are many differences in their pathogenesis. To date, SFP has been widespread throughout the world and is an urgent problem, although exact epidemiol. data on incidence varies greatly. The need to distinguish SFP from true IgE-assocd. allergy to fish and seafood is one of the most difficult examples of the differential diagnosis of allergic conditions. The most important difference is the absence of an IgE response in SFP. The pathogenesis of SFP includes a complex system of interactions between the body and chem. triggers such as exogenous histamine, other biogenic amines, cis-urocanic acid, salicylates, and other histamine liberators. Because of the wide range of mol. pathways involved in this process, it is crit. to understand their differences. This may help predict and prevent poor outcomes in patients and contribute to the development of adequate hygienic rules and regulations for seafood product safety. Despite the vast and lengthy history of research on SFP mechanisms, there are still many blank spots in our understanding of this condition. The goals of this review are to differentiate various mol. mechanisms of SFP and describe methods of hygienic regulation of some biogenic amines that influence the concn. of histamine in the human body and play an important role in the mechanism of SFP.
- 9Feng, C.; Teuber, S.; Gershwin, M. E. Histamine (Scombroid) Fish Poisoning: A Comprehensive Review. Clin Rev. Allergy Immunol 2016, 50 (1), 64– 69, DOI: 10.1007/s12016-015-8467-x9https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2MXhvFGksro%253D&md5=d7183abbc805b94eb0873f0712f83effHistamine (Scombroid) Fish Poisoning: a Comprehensive ReviewFeng, Charles; Teuber, Suzanne; Gershwin, M. EricClinical Reviews in Allergy & Immunology (2016), 50 (1), 64-69CODEN: CRAIF2; ISSN:1080-0549. (Springer)A review. Histamine fish poisoning, also known as scombroid poisoning, is the most common cause of ichythyotoxicosis worldwide and results from the ingestion of histamine-contaminated fish in the Scombroidae and Scomberesocidae families, including mackerel, bonito, albacore, and skipjack. This disease was first described in 1799 in Britain and re-emerged in the medical literature in the 1950s when outbreaks were reported in Japan. The symptoms assocd. with histamine fish poisoning are similar to that of an allergic reaction. In fact, such histamine-induced reactions are often misdiagnosed as IgE-mediated fish allergy. Indeed, histamine fish poisoning is still an underrecognized disease. In this review, we discuss the epidemiol., pathophysiol., evaluation, and treatment of scombroid disease. Because more than 80 % of fish consumed in the USA is now imported from other countries, the disease is intimately linked with the global fish trade (National Marine Fisheries Service, 2012). Preventing future scombroid outbreaks will require that fishermen, public health officials, restaurant workers, and medical professionals work together to devise international safety stds. and increase awareness of the disease. The implications of scombroid poisoning go far beyond that of fish and have broader implications for the important issues of food safety.
- 10Schirone, M.; Visciano, P.; Tofalo, R.; Suzzi, G.; Hattori, Y.; Seifert, R.; Schirone, M.; Visciano, P.; Tofalo, R.; Suzzi, G. Histamine Food Poisoning. In Histamine and Histamine Receptors in Health and Disease; Springer: Cham, 2016; pp. 217– 235.There is no corresponding record for this reference.
- 11Zhao, Y.; Zhang, X.; Jin, H.; Chen, L.; Ji, J.; Zhang, Z. Histamine Intolerance─A Kind of Pseudoallergic Reaction. Biomolecules 2022, 12 (3), 454, DOI: 10.3390/biom1203045411https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XoslKlt74%253D&md5=8454ac794eb199a401e299dec264edf2Histamine Intolerance-A Kind of Pseudoallergic ReactionZhao, Ying; Zhang, Xiaoyan; Jin, Hengxi; Chen, Lu; Ji, Jiang; Zhang, ZhongweiBiomolecules (2022), 12 (3), 454CODEN: BIOMHC; ISSN:2218-273X. (MDPI AG)A review. Histamine intolerance (HIT) is a common disorder assocd. with impaired histamine metab. Notwithstanding, it is often misdiagnosed as other diseases because of its lack of specific clin. manifestations. HIT did not gain traction until the early 21st century. In this review, we will focus on the latest research and elaborate on the clin. manifestations of HIT, including its manifestations in special populations such as atopic dermatitis (AD) and chronic urticaria (CU), as well as the latest understanding of its etiol. and pathogenesis. In addn., we will explore the latest treatment strategies for HIT and the treatment of specific cases.
- 12Chen, X.; Zhang, D.; Liu, Q.; Liu, S.; Li, H.; Li, Z. Enzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne Allergens. Anal. Chem. 2023, 95 (18), 7354– 7362, DOI: 10.1021/acs.analchem.3c0059112https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXosVaks70%253D&md5=6e4081a47a37f81a66ebc8da83b3166aEnzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne AllergensChen, Xiaofeng; Zhang, Dongdong; Liu, Qingmei; Liu, Sihui; Li, Houlin; Li, ZhengAnalytical Chemistry (Washington, DC, United States) (2023), 95 (18), 7354-7362CODEN: ANCHAM; ISSN:0003-2700. (American Chemical Society)Food allergy has become a growing health concern that may impair life quality and even cause life-threatening outcomes. Accidental and continuous exposure to allergenic bioaerosols has a substantially neg. impact on the respiratory health of patients. Traditional anal. methodologies for food allergens are restricted by strong reliance on bulk instrumentation and skilled personnel, particularly in low-resource settings. In this study, a fluorescent sensor array based on the ELISA performed on a herringbone-shaped microfluidic chip (ELISA-HB-chip) was designed for dynamically sensitive and multiplexed quantification of foodborne allergens in aerosols that originated from liq. food exts. Due to the high surface area of aerosol particles and sufficient mixing of immunol. reagents using a herringbone micromixer, the detection sensitivity was improved by over an order of magnitude compared to traditional allergen detection in the aq. phase. Through fluorescence imaging of multiple regions on the ELISA-HB-chip, four important foodborne allergens, namely, ovalbumin, ovomucoid, lysozyme, and tropomyosin, could be simultaneously monitored without any cross-reactivity, and the limits of detection for these allergenic species were detd. to be 7.8, 1.2, 4.2, and 0.31 ng/mL, resp. Combining with a 3D printed and portable fluorescence microscope, this platform exhibited an excellent field-deployable capacity for quick and accurate detn. of allergens in the aerosol state from spiked buffer solns., thus displaying the practicality for food safety screening at cooking or food processing sites where patients are potentially under exposure to allergenic bioaerosols that escaped from food matrixes or exts.
- 13Munir, M. A.; Mackeen, M. M. M.; Heng, L. Y.; Badri, K. H. Study of Histamine Detection Using Liquid Chromatography and Gas Chromatography. ASM Science Journal 2021, 16, 1– 9, DOI: 10.32802/asmscj.2021.809There is no corresponding record for this reference.
- 14Zhang, B.; Cai, D.; Lang, Y.; Lin, X.; Yang, K.; Shentu, X.; Yu, X. A Smartphone-Integrated Multi-Model Thermal Immunochromatographic Assay for Sensitive Detection of Histamine in Real Samples. Sens Actuators B Chem. 2023, 394, 134474 DOI: 10.1016/j.snb.2023.13447414https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhsleku7fN&md5=13456e2fe1ea08a6c2ae3eea3cd6afebA smartphone-integrated multi-model thermal immunochromatographic assay for sensitive detection of histamine in real samplesZhang, Biao; Cai, Danfeng; Lang, Yihan; Lin, Xiaodong; Yang, Ke; Shentu, Xuping; Yu, XiaopingSensors and Actuators, B: Chemical (2023), 394 (), 134474CODEN: SABCEB; ISSN:0925-4005. (Elsevier B.V.)A smartphone-integrated multi-model thermal immunochromatog. assay has been constructed for the detection of histamine by using anti-histamine antibody coupled Cu2-xSe nanoparticles (Cu2-xSe@antibody) as photothermal nanoprobes. Base on a competitive format, the coated histamine antigen on T-line and histamine simultaneously competed for binding the lateral flow photothermal signal probes. With the increase of histamine concn., the temp. of Cu2-xSe@antibody on T-line was decreased under the 808 nm laser irradn., which can be monitored via the smartphone-thermal imager combination device. In addn., the color on T-line from nanoprobes gradually disappeared, and the inhibition rate calcd. from the obtained temp. on T-line was increased. The smartphone-integrated multi-model thermal immunochromatog. assay enables detection of histamine in the range of 0.1-10.0μg/L with limit of detection as low as 0.09μg/L. The histamine concns. in crab, tuna, saury, mackerel, shrimp were 5.67 mg/kg, 83.73 mg/kg, 14.03 mg/kg, 21.30 mg/kg, 3.22 mg/kg detected by the developed thermal immunochromatog. assay, resp. The developed multi-model strategy provided good sensitivity, simple operation, effectiveness and practicability for histamine in real samples compared to ultra-performance liq. chromatog.-tandem mass spectrometry UPLC-MS/MS and ELISA.
- 15Xu, L.; Zhou, J.; Eremin, S.; Dias, A. C. P.; Zhang, X. Development of ELISA and Chemiluminescence Enzyme Immunoassay for Quantification of Histamine in Drug Products and Food Samples. Anal Bioanal Chem. 2020, 412, 4739– 4747, DOI: 10.1007/s00216-020-02730-515https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXhtVOksL%252FF&md5=f5812a1014fdb3d27029be4118c6f0c7Development of ELISA and chemiluminescence enzyme immunoassay for quantification of histamine in drug products and food samplesXu, Long; Zhou, Jiping; Eremin, Sergei; Dias, Alberto C. P.; Zhang, XiaoyingAnalytical and Bioanalytical Chemistry (2020), 412 (19), 4739-4747CODEN: ABCNBP; ISSN:1618-2642. (Springer)Histamine (HA) is a biogenic amine assocd. with allergies and food poisoning. It is an important indicator of food freshness and quality. In recent years, a series of medical negligence cases have been reported to be related to the i.v. injection of antibiotics produced via fermn. with fish peptone due to HA contamination. To detect HA efficiently, mouse monoclonal antibody was developed. An ELISA and a chemiluminescence enzyme immunoassay (CLEIA) were developed and compared with conventional HPLC anal. Both immunoassays showed low cross-reactivity, low 50% inhibitive concn. (IC50; 1.2μg/mL and 1.1μg/mL), low limits of detection (LODs, IC10; 89.0 ng/mL and 73.4 ng/mL), and appreciable recoveries in spiked foods and drugs (from 73.4 to 131.0% and from 77.0 to 119.0%, espectively), demonstrating that the developed methods are sensitive, specific, fast, and reliable for HA detection in complicated real samples.
- 16Zhang, M.; Li, M.; Zhao, Y.; Xu, N.; Peng, L.; Wang, Y.; Wei, X. Novel Monoclonal Antibody-Sandwich Immunochromatographic Assay Based on Fe3O4/Au Nanoparticles for Rapid Detection of Fish Allergen Parvalbumin. Food Research International 2021, 142, 110102 DOI: 10.1016/j.foodres.2020.11010216https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXksl2qtr4%253D&md5=9c228941c75036e6cd17103a739fd7afNovel monoclonal antibody-sandwich immunochromatographic assay based on Fe3O4/Au nanoparticles for rapid detection of fish allergen parvalbuminZhang, Mengke; Li, Mengyin; Zhao, Yan; Xu, Naifeng; Peng, Lanlan; Wang, Yuanfeng; Wei, XinlinFood Research International (2021), 142 (), 110102CODEN: FORIEU; ISSN:0963-9969. (Elsevier B.V.)In this study, a rapid sandwich immunochromatog. assay (ICA) was developed to detect parvalbumin (PV). Firstly, two optimum primary monoclonal antibody (mAb) against PV had been screened out: mAb1 was used as the capture antibody, and mAb2 conjugated to Fe3O4/Au nanoparticles (Fe3O4/AuNPs) that served as a detection reagent. Using this pair of mAbs, a sandwich ICA strip based on Fe3O4/AuNPs was developed. The results showed that the color intensity of test line pos. correlated with the PV concn. in the std. or spiked sample. The limit of detection for qual. (LOD) and quant. detection (LOQ) were 2 ng/mL and 0.691 ng/mL, resp. Besides, the detection time of this ICA strip was within 15 min. The recovery rates ranged from 104.0% to 117.4%, within an acceptable level (80-120%). Moreover, the developed assay also showed high cross reaction in different fish species. These results demonstrated that the established test strip has the potential to be used as a rapid screening tool for large scale detn. of PV in foodstuffs.
- 17Cai, Q. F.; Wang, X. C.; Liu, G. M.; Zhang, L.; Ruan, M. M.; Liu, Y.; Cao, M. J. Development of a Monoclonal Antibody-Based Competitive Enzyme Linked-Immunosorbent Assay (c-ELISA) for Quantification of Silver Carp Parvalbumin. Food Control 2013, 29 (1), 241– 247, DOI: 10.1016/j.foodcont.2012.06.01617https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC38XhtlGqt77O&md5=33a6ef5940a93a176cfb03b99ede949dDevelopment of a monoclonal antibody-based competitive enzyme linked-immunosorbent assay (c-ELISA) for quantification of silver carp parvalbuminCai, Qiu-Feng; Wang, Xi-Chang; Liu, Guang-Ming; Zhang, Lin; Ruan, Mi-Mi; Liu, Yuan; Cao, Min-JieFood Control (2013), 29 (1), 241-247CODEN: FOOCEV; ISSN:0956-7135. (Elsevier Ltd.)Parvalbumin (PV) is a major allergen in fish. A monoclonal antibody (B2-E1) against silver carp parvalbumin was prepd. in the present study. Western blot anal. indicated that the prepd. monoclonal antibody was specific to PV in fish muscle ext. A competitive ELISA (c-ELISA) was then developed to quantify the amt. of PV in silver carp using B2-E1. The limit of detection (LOD) and the limit of quantification (LOQ) of the developed c-ELISA were 0.04 and 0.3 mg/kg food, resp. The c-ELISA was specific for detecting PV from fish, and the intra- and inter-assay coeffs. of variation were 4.4% and 8.4%, resp. The c-ELISA method was further applied to quantify PV from various fish species, and demonstrated that the PV content was 4-20 times higher in the white muscle than that in the dark muscle, which was coincident with the previous reports. Moreover, c-ELISA was used to trace the PV during fish surimi (fish ball) prodn., and the results indicated that washing process could effectively but not completely remove PV from fish ball. Our present study indicated that this c-ELISA method may be applied to monitor trace amts. of fish allergen PV during fishery product processing.
- 18Mukherjee, S.; Horka, P.; Zdenkova, K.; Cermakova, E. Parvalbumin: A Major Fish Allergen and a Forensically Relevant Marker. Genes 2023, 14, 223, DOI: 10.3390/genes1401022318https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhvFylt7g%253D&md5=9f9456cfbe43b54438893385e8a516abParvalbumin: A Major Fish Allergen and a Forensically Relevant MarkerMukherjee, Subham; Horka, Petra; Zdenkova, Kamila; Cermakova, EliskaGenes (2023), 14 (1), 223CODEN: GENEG9; ISSN:2073-4425. (MDPI AG)A review. Parvalbumins (PVALBs) are low mol. wt. calcium-binding proteins. In addn. to their role in many biol. processes, PVALBs play an important role in regulating Ca2+ switching in muscles with fast-twitch fibers in addn. to their role in many biol. processes. The PVALB gene family is divided into two gene types, alpha (α) and beta (β), with the β gene further divided into two gene types, beta1 (β1) and beta2 (β2), carrying traces of whole genome duplication. A large variety of commonly consumed fish species contain PVALB proteins which are known to cause fish allergies. More than 95% of all fish-induced food allergies are caused by PVALB proteins. The authentication of fish species has become increasingly important as the seafood industry continues to grow and the growth brings with it many cases of food fraud. Since the PVALB gene plays an important role in the initiation of allergic reactions, it has been used for decades to develop alternate assays for fish identification. A brief review of the significance of the fish PVALB genes is presented in this article, which covers evolutionary diversity, allergic properties, and potential use as a forensic marker.
- 19Saptarshi, S. R.; Sharp, M. F.; Kamath, S. D.; Lopata, A. L. Antibody Reactivity to the Major Fish Allergen Parvalbumin Is Determined by Isoforms and Impact of Thermal Processing. Food Chem. 2014, 148, 321– 328, DOI: 10.1016/j.foodchem.2013.10.03519https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3sXhsl2lsLvI&md5=b3abc39a58395137530f23879a5ca28fAntibody reactivity to the major fish allergen parvalbumin is determined by isoforms and impact of thermal processingSaptarshi, Shruti R.; Sharp, Michael F.; Kamath, Sandip D.; Lopata, Andreas L.Food Chemistry (2014), 148 (), 321-328CODEN: FOCHDJ; ISSN:0308-8146. (Elsevier Ltd.)The EF-hand calcium binding protein, parvalbumin, is a major fish allergen. Detection of this allergen is often difficult due to its structural diversity among various fish species. The aim of this study was to evaluate the cross-reactivity of parvalbumin in a comprehensive range of bony and cartilaginous fish, from the Asia-Pacific region, and conduct a mol. anal. of this highly allergenic protein. Using the monoclonal anti-parvalbumin antibody PARV-19, we demonstrated the presence of monomeric and oligomeric parvalbumin in all fish analyzed, except for gummy shark, a cartilaginous fish. Heat processing of this allergen greatly affected its antibody reactivity. While heating caused a redn. in antibody reactivity to multimeric forms of parvalbumins for most bony fish, a complete loss of reactivity was obsd. for cartilaginous fish. Mol. anal. demonstrated that parvalbumin cross-reactivity, among fish species, is due to the mol. phylogenetic assocn. of this major fish allergen.
- 20Nevado, D. L.; Santos, S. D.; Bastian, G.; Deyta, J.; Managuelod, E. J.; Fortaleza, J. A.; De Jesus, R. Detection, Identification, and Inactivation of Histamine-Forming Bacteria in Seafood: A Mini-Review. J. Food Prot. 2023, 86 (3), 100049 DOI: 10.1016/j.jfp.2023.10004920https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB28bmsFGrtg%253D%253D&md5=6cc04786e61dc780bdbf1e143aded94bDetection, Identification, and Inactivation of Histamine-forming Bacteria in Seafood: A Mini-reviewNevado Daniel Lance; Delos Santos Sophia; Bastian Gelian; Deyta Jimson; Managuelod El-Jay; Fortaleza Jamil Allen; De Jesus RenerJournal of food protection (2023), 86 (3), 100049 ISSN:.Seafood is one of the essential sources of nutrients for the human diet. However, they can be subject to contamination and can cause foodborne illnesses, including scombroid fish poisoning caused by histamine. Many microorganisms can produce enzymes that eventually decompose endogenous histidine to histamine in postmortem fish muscles and tissues. One of these is histamine-forming bacteria (HFB), primarily found in the gills, gut, and skin of fishes. Previous studies linked a plethora of Gram-negative HFB including Morganella spp. and Photobacterium spp. to scombroid fish poisoning from many types of seafood, especially the Scombridae family. These bacteria possess the hdc gene to produce histidine decarboxylase enzyme. It was reported that Gram-negative HFB produced 6345 ppm in tuna and 1223 ppm in Spanish mackerel. Interestingly, Gram-positive HFB have been isolated in the seafood samples with lower histamine levels. It suggests that Gram-negative HFB are the major contributor to the accumulation of histamine in seafood. Several analytical methods are available to detect and identify HFB and their histamine metabolites from seafood substrates. Rapid test kits can be used in food production settings for early detection of histamine to avoid food intoxication. Furthermore, high hydrostatic pressure and irradiation treatment could prevent the proliferation of HFB and inactivate the existing histidine decarboxylase (HDC) activity. As demonstrated in different seafood model systems, the HDC activity was deactivated at a maximum high hydrostatic pressure level of 400 MPa. The complete inactivation of HFB was achieved by gamma irradiation at a dose of 4.0 kGy. Other postharvest treatments, like enzymatic degradation and electrolyzed oxidizing water, were studied as sustainable methods for bacterial growth prevention and enzyme inactivation. However, other HFB react differently to these treatment conditions, and further studies are recommended.
- 21Visciano, P.; Schirone, M.; Tofalo, R.; Suzzi, G. Histamine Poisoning and Control Measures in Fish and Fishery Products. Front Microbiol 2014, 5, 1– 3, DOI: 10.3389/fmicb.2014.00500There is no corresponding record for this reference.
- 22Kobayashi, Y.; Huge, J.; Imamura, S.; Hamada-Sato, N. Study of the Cross-Reactivity of Fish Allergens Based on a Questionnaire and Blood Testing. Allergology International 2016, 65 (3), 272– 279, DOI: 10.1016/j.alit.2016.01.00222https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2sXitVWlurnF&md5=8689e82073c039890780ebd4c35db6b6Study of the cross-reactivity of fish allergens based on a questionnaire and blood testingKobayashi, Yukihiro; Huge, Jiletu; Imamura, Shintaro; Hamada-Sato, NaokoAllergology International (2016), 65 (3), 272-279CODEN: ALINFR; ISSN:1323-8930. (Japanese Society of Allergology)Background: Parvalbumin and collagen have been identified as cross-reactive allergens for fish allergies. Although doctors realize that various fish elicit allergies, the targets of food allergen labeling laws were only mackerels and salmons in Japan and mackerels in South Korea. This study aimed to reveal the causative species for fish allergy via questionnaires and blood tests. Methods: Questionnaire research was conducted in Japan via the internet concerning allergies for fish-allergic patients or their family members. Next, IgE reactivities and cross-reactivities of 26 fish species were analyzed using sera obtained from 16 Japanese patients who were allergic to fish parvalbumin or collagen by ELISA (ELISA) and inhibition ELISA. Results: Questionnaire research revealed that 88% patients cannot eat mackerel and salmon in addn. to other fish. In addn., 85% respondents were not satisfied with the current food allergen labeling law. In ELISA analyses, we clarified that pooled serum obtained from patients with fish parvalbumin-specific allergies exhibited IgE reactivity to the exts. of most fish species, and pooled serum obtained from patients with fish collagen-specific allergies displayed IgE reactivity to the exts. of all types of fish. Inhibition ELISA expts. revealed cross-reactivities of parvalbumin or collagen to exts. from all fish tested. Conclusions: Most patients with fish allergies displayed allergic symptoms following the intake of various fish species. In addn., fish parvalbumin and collagen were causative factors of fish allergy and were highly cross-reactive fish panallergens. Therefore, current laws should be revised in Japan and South Korea.
- 23Yang, H.; Min, J.; Han, X.-Y.; Li, X.-Y.; Hu, J.-W.; Liu, H.; Cao, M.-J.; Liu, G.-M. Reduction of the Histamine Content and Immunoreactivity of Parvalbumin in Decapterus Maruadsi by a Maillard Reaction Combined with Pressure Treatment. Food Funct. 2018, 9, 4897– 4905, DOI: 10.1039/c8fo01167b23https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1cXhsF2jsbbO&md5=6d30682375b7e1045e234f038f0b8365Reduction of the histamine content and immunoreactivity of parvalbumin in Decapterus maruadsi by a Maillard reaction combined with pressure treatmentYang, Huang; Min, Juan; Han, Xin-Yu; Li, Xiao-Yan; Hu, Jia-Wei; Liu, Hong; Cao, Min-Jie; Liu, Guang-MingFood & Function (2018), 9 (9), 4897-4905CODEN: FFOUAI; ISSN:2042-6496. (Royal Society of Chemistry)The aim of this study was to develop an effective method for decreasing the content of histamine and the immunoreactivity of parvalbumin in Decapterus maruadsi. As demonstrated by reverse phase high performance liq. chromatog., no effect on histamine content was found when fish were treated by boiling (100 °C), ultrasonication, UV irradn., pressure treatment (121 °C, 0.12 MPa). However, the histamine content was reduced by 73.55% when the Maillard reaction was combined with pressure treatment (MPT). Further, the allergenicity of parvalbumin was retained after boiling, ultrasonication and UV irradn., but was effectively decreased when fish were treated by MPT. Animal exptl. results showed lower levels of IgE, IgG1 and IgG2a and contents of serum histamine when measured in a group of MPT sensitized mice. These results showed that the MPT is an effective method for simultaneously reducing the histamine content and the immunoreactivity of parvalbumin from Decapterus maruadsi.
- 24Liu, Y.; Zhan, L.; Qin, Z.; Sackrison, J.; Bischof, J. C. Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis. ACS Nano 2021, 15 (3), 3593– 3611, DOI: 10.1021/acsnano.0c1003524https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXksVanurs%253D&md5=de016e660dce04b0e518d73181c2cf90Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care DiagnosisLiu, Yilin; Zhan, Li; Qin, Zhenpeng; Sackrison, James; Bischof, John C.ACS Nano (2021), 15 (3), 3593-3611CODEN: ANCAC3; ISSN:1936-0851. (American Chemical Society)A review. Lateral flow assays (LFAs) are paper-based point-of-care (POC) diagnostic tools that are widely used because of their low cost, ease of use, and rapid format. Unfortunately, traditional com. LFAs have significantly poorer sensitivities (μM) and specificities than std. lab. tests (ELISA: pM-fM; polymerase chain reaction, PCR: aM), thus limiting their impact in disease control. In this Perspective, we review the evolving efforts to increase the sensitivity and specificity of LFAs. Recent work to improve the sensitivity through assay improvement includes optimization of the assay kinetics and signal amplification by either reader systems or addnl. reagents. Together, these efforts have produced LFAs with ELISA-level sensitivities (pM-fM). In addn., sample preamplification can be applied to both nucleic acids (direct amplification) and other analytes (indirect amplification) prior to LFA testing, which can lead to PCR-level (aM) sensitivity. However, these amplification strategies also increase the detection time and assay complexity, which inhibits the large-scale POC use of LFAs. Perspectives to achieve future rapid (<30 min), ultrasensitive (PCR-level), and "sample-to-answer" POC diagnostics are also provided. In the case of LFA specificity, recent research efforts have focused on high-affinity mols. and assay optimization to reduce nonspecific binding. Furthermore, novel highly specific mols., such as CRISPR/Cas systems, can be integrated into diagnosis with LFAs to produce not only ultrasensitive but also highly specific POC diagnostics. In summary, with continuing improvements, LFAs may soon offer performance at the POC that is competitive with lab. techniques while retaining a rapid format.
- 25Sena-Torralba, A.; Álvarez-Diduk, R.; Parolo, C.; Piper, A.; Merkoçi, A. Toward Next Generation Lateral Flow Assays: Integration of Nanomaterials. Chem. Rev. 2022, 122 (18), 14881– 14910, DOI: 10.1021/acs.chemrev.1c0101225https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XitlSlu77J&md5=d0e1f585d8de5c5c880ab849aaf82e3cToward Next Generation Lateral Flow Assays: Integration of NanomaterialsSena-Torralba, Amadeo; Alvarez-Diduk, Ruslan; Parolo, Claudio; Piper, Andrew; Merkoci, ArbenChemical Reviews (Washington, DC, United States) (2022), 122 (18), 14881-14910CODEN: CHREAY; ISSN:0009-2665. (American Chemical Society)A review. Lateral flow assays (LFAs) are currently the most used point-of-care sensors for both diagnostic (e.g., pregnancy test, COVID-19 monitoring) and environmental (e.g., pesticides and bacterial monitoring) applications. Although the core of LFA technol. was developed several decades ago, in recent years the integration of novel nanomaterials as signal transducers or receptor immobilization platforms has brought improved anal. capabilities. In this Review, we present how nanomaterial-based LFAs can address the inherent challenges of point-of-care (PoC) diagnostics such as sensitivity enhancement, lowering of detection limits, multiplexing, and quantification of analytes in complex samples. Specifically, we highlight the strategies that can synergistically solve the limitations of current LFAs and that have proven com. feasibility. Finally, we discuss the barriers toward commercialization and the next generation of LFAs.
- 26Posthuma-Trumpie, G. A.; Korf, J.; van Amerongen, A. Lateral Flow (Immuno)Assay: Its Strengths, Weaknesses, Opportunities and Threats A Literature Survey. Anal Bioanal Chem. 2009, 393 (2), 569– 582, DOI: 10.1007/s00216-008-2287-226https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BD1cXhsFWit77M&md5=6bfd3157ea4aefdccf24bd45eff6dc09Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature surveyPosthuma-Trumpie, Geertruida A.; Korf, Jakob; van Amerongen, AartAnalytical and Bioanalytical Chemistry (2009), 393 (2), 569-582CODEN: ABCNBP; ISSN:1618-2642. (Springer)A review. Lateral flow (immuno)assays are currently used for qual., semiquant. and to some extent quant. monitoring in resource-poor or non-lab. environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quant. monitoring. We illustrate the potentials and limitations of anal. with lateral flow (immuno)assays using a literature survey and a SWOT anal. (acronym for "strengths, weaknesses, opportunities, threats"). Articles referred to in this survey were searched for on MEDLINE, Scopus and in refs. of reviewed papers. Search terms included "immunochromatog.", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".
- 27Boehringer, H. R.; O’Farrell, B. J. Lateral Flow Assays in Infectious Disease Diagnosis. Clin Chem. 2021, 68 (1), 52– 58, DOI: 10.1093/clinchem/hvab19427https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB2M%252FitFGntQ%253D%253D&md5=5b0b6da270c6c3c97e88b15d6da9a090Lateral Flow Assays in Infectious Disease DiagnosisBoehringer Hans R; O'Farrell Brendan JClinical chemistry (2021), 68 (1), 52-58 ISSN:.BACKGROUND: Lateral flow immunoassays are widely used as diagnostic tests in many applications in human and other diagnostic areas. Assays for human applications have been commercially available since the 1980s and initially were primarily used to identify pregnancy by measuring human chorionic gonadotropin in urine and serum/plasma. CONTENT: The first infectious disease lateral flow assays were commercialized in the late 1980s identifying the presence of Group A Streptococcus pyogenes collected with throat swabs; innumerable other applications followed in the intervening decades. The severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) pandemic has brought a vast number of new assays for which emergency use authorization (EUA) has been requested in the USA. These assays have been designed for detection of the antibody response to an infection and viral antigens in respiratory samples. In view of the onslaught of new tests, this review will focus on the use of rapid lateral flow immunoassays for infectious diseases. Principles of lateral flow assays and approaches to the production of high-sensitivity point-of-care assays are presented. Market trends, customer requirements, and future directions of lateral flow assay technology and its applications in the infectious disease diagnostic space are discussed. SUMMARY: Lateral flow immunoassays play an important role in infectious disease diagnostics. Advancements in technology have led to improved performance of these assays and acceptance by professional users. With the advent of the SARS-CoV-2 pandemic, the market has reached new levels requiring hundreds of millions of tests per year for professional and even home use.
- 28Kim, J.; Shin, M.-S.; Shin, J.; Kim, H.-M.; Pham, X.-H.; Park, S.; Kim, D.-E.; Kim, Y. J.; Jun, B.-H. Recent Trends in Lateral Flow Immunoassays with Optical Nanoparticles. Int. J. Mol. Sci. 2023, 24 (11), 9600, DOI: 10.3390/ijms2411960028https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXht1CnsLvF&md5=3bf6fb2e85b1457adfda9db0be971994Recent Trends in Lateral Flow Immunoassays with Optical NanoparticlesKim, Jaehi; Shin, Min-Sup; Shin, Jonghyun; Kim, Hyung-Mo; Pham, Xuan-Hung; Park, Seung-min; Kim, Dong-Eun; Kim, Young Jun; Jun, Bong-HyunInternational Journal of Molecular Sciences (2023), 24 (11), 9600CODEN: IJMCFK; ISSN:1422-0067. (MDPI AG)A review. Rapid, accurate, and convenient diagnosis is essential for effective disease management. Various detection methods, such as ELISA, have been extensively used, with lateral flow immunoassay (LFIA) recently emerging as a major diagnostic tool. Nanoparticles (NPs) with characteristic optical properties are used as probes for LFIA, and researchers have presented various types of optical NPs with modified optical properties. Herein, we review the literature on LFIA with optical NPs for the detection of specific targets in the context of diagnostics.
- 29Liang, P.; Guo, Q.; Zhao, T.; Wen, C.-Y.; Tian, Z.; Shang, Y.; Xing, J.; Jiang, Y.; Zeng, J. Ag Nanoparticles with Ultrathin Au Shell-Based Lateral Flow Immunoassay for Colorimetric and SERS Dual-Mode Detection of SARS-CoV-2 IgG. Anal. Chem. 2022, 94 (23), 8466– 8473, DOI: 10.1021/acs.analchem.2c0128629https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XhsVagsrvK&md5=34ef55dcb8d60f9c5b1c53ddfec70df6Ag Nanoparticles with Ultrathin Au Shell-Based Lateral Flow Immunoassay for Colorimetric and SERS Dual-Mode Detection of SARS-CoV-2 IgGLiang, Penghui; Guo, Qi; Zhao, Tianyu; Wen, Cong-Ying; Tian, Zhangyu; Shang, Yanxue; Xing, Jinyan; Jiang, Yongzhong; Zeng, JingbinAnalytical Chemistry (Washington, DC, United States) (2022), 94 (23), 8466-8473CODEN: ANCHAM; ISSN:0003-2700. (American Chemical Society)Ig detection is essential for diagnosing progression of SARS-CoV-2 infection, for which SARS-CoV-2 IgG is one of the most important indexes. In this paper, Ag nanoparticles with ultrathin Au shells (∼2 nm) embedded with 4-mercaptobenzoic acid (MBA) (AgMBA@Au) were manufd. via a ligand-assisted epitaxial growth method and integrated into lateral flow immunoassay (LFIA) for colorimetric and SERS dual-mode detection of SARS-CoV-2 IgG. AgMBA@Au possessed not only the surface chem. advantages of Au but also the superior optical characteristics of Ag. Moreover, the nanogap between the Ag core and the Au shell also greatly enhanced the Raman signal. After being modified with anti-human antibodies, AgMBA@Au recognized and combined with SARS-CoV-2 IgG, which was captured by the SARS-CoV-2 spike protein on the T line. Qual. anal. was achieved by visually observing the color of the T line, and quant. anal. was conducted by measuring the SERS signal with a sensitivity 4 orders of magnitude higher (detection limit: 0.22 pg/mL). The intra-assay and inter-assay variation coeffs. were 7.7 and 10.3%, resp., and other proteins at concns. 10-20-fold higher than those of SARS-CoV-2 IgG could hardly produce distinguishable signals, confirming good reproducibility and specificity. Finally, this method was used to detect 107 clin. serum samples. The results agreed well with those obtained from ELISA kits and were significantly better than those of the colloidal gold test strips. Therefore, this dual-mode LFIA has great potential in clin. practical applications and can be used to screen and trace the early immune response of SARS-CoV-2.
- 30Joung, Y.; Kim, K.; Lee, S.; Chun, B.-S.; Lee, S.; Hwang, J.; Choi, S.; Kang, T.; Lee, M.-K.; Chen, L.; Choo, J. Rapid and Accurate On-Site Immunodiagnostics of Highly Contagious Severe Acute Respiratory Syndrome Coronavirus 2 Using Portable Surface-Enhanced Raman Scattering-Lateral Flow Assay Reader. ACS Sens 2022, 7 (11), 3470– 3480, DOI: 10.1021/acssensors.2c0180830https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38XivVKjsLbN&md5=6b7ac8e90aac6cf7553a6fdb52f2f301Rapid and Accurate On-Site Immunodiagnostics of Highly Contagious Severe Acute Respiratory Syndrome Coronavirus 2 Using Portable Surface-Enhanced Raman Scattering-Lateral Flow Assay ReaderJoung, Younju; Kim, Kihyun; Lee, Seungwoo; Chun, Byung-Seon; Lee, Sangyeop; Hwang, Joonki; Choi, Suji; Kang, Taejoon; Lee, Mi-Kyung; Chen, Lingxin; Choo, JaebumACS Sensors (2022), 7 (11), 3470-3480CODEN: ASCEFJ; ISSN:2379-3694. (American Chemical Society)In early 2022, the no. of people infected with the highly contagious mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), called Omicron, was increasing worldwide. Therefore, several countries approved the lateral flow assay (LFA) strip as a diagnostic method for confirming SARS-CoV-2 instead of reverse transcription-polymerase chain reaction (RT-PCR), which takes a long time to generate the results. However, owing to the limitation of detection sensitivity, com. LFA strips have high false-neg. diagnosis rates for patients with low virus concns. Therefore, in this study, we developed a portable surface-enhanced Raman scattering (SERS)-LFA reader based on localized surface plasmon effects to solve the sensitivity problem of the com. LFA strip. We tested 54 clin. samples using this portable SERS-LFA reader, which generated 49 pos. and 5 neg. results. Out of the 49 pos. results, SERS-LFA classified only 2 as false neg., while the com. LFA classified 21 as false neg. This confirmed that the false-neg. rate had significantly improved compared to that of com. LFA strips. We believe that the proposed SERS-LFA system can be utilized as a point-of-care diagnostic system to quickly and accurately det. a virus infection that could spread significantly within a short period.
- 31Castrejón-Jiménez, N. S.; García-Pérez, B. E.; Reyes-Rodríguez, N. E.; Vega-Sánchez, V.; Martínez-Juárez, V. M.; Hernández-González, J. C. Challenges in the Detection of SARS-CoV-2: Evolution of the Lateral Flow Immunoassay as a Valuable Tool for Viral Diagnosis. Biosensors (Basel) 2022, 12 (9), 728, DOI: 10.3390/bios12090728There is no corresponding record for this reference.
- 32Huang, L.; Tian, S.; Zhao, W.; Liu, K.; Ma, X.; Guo, J. Multiplexed Detection of Biomarkers in Lateral-Flow Immunoassays. Analyst 2020, 145 (8), 2828– 2840, DOI: 10.1039/C9AN02485A32https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXktFWhtLc%253D&md5=54d963c73d4a14be0378e8aadc49f263Multiplexed detection of biomarkers in lateral-flow immunoassaysHuang, Lei; Tian, Shulin; Zhao, Wenhao; Liu, Ke; Ma, Xing; Guo, JinhongAnalyst (Cambridge, United Kingdom) (2020), 145 (8), 2828-2840CODEN: ANALAO; ISSN:0003-2654. (Royal Society of Chemistry)Multiplexed detection of biomarkers, i.e., simultaneous detection of multiple biomarkers in a single assay, is a process of great advantages including enhanced diagnostic precision, improved diagnostic efficiency, reduced diagnostic cost, and alleviated pain of patients. A typical lateral-flow immunoassay (LFIA) is a widely used paper-based immunochromatog. test strip designed to detect a target biomarker through two common formats: sandwich assay and competitive assay. In order to obtain qual. or quant. results, a probe with unique optical or magnetic properties is usually employed to characterize the concn. of the target biomarker. The typical LFIA is suitable for point-of-care testing due to its simplicity, portability, cost-effectiveness, and rapid detection of a target biomarker. However, detection of a single biomarker in the typical LFIA is not favorable for high throughput anal. Therefore, multiplexed detection of biomarkers in LFIAs has been extensively studied in recent years for high throughput anal. To accomplish multiplexed detection of biomarkers in LFIAs, the most frequently used structure is a test strip with multiple test lines (TLs), where each TL can detect a specific biomarker. An alternative structure, i.e., a multi-channel structure with multiple test strips, where each test strip has one TL for detecting a specific biomarker, is employed for multiplexed detection of biomarkers. Sometimes, a single test strip with only one TL contg. different receptors, where each detection receptor corresponds to a specific biomarker, is another structure applied for multiplexed detection of biomarkers. This paper reviews three common structures for multiplexed detection of biomarkers in LFIAs, i.e., a test strip with multiple TLs, a multi-channel structure with multiple test strips, and a test strip with a single TL. Based on the three common structures, different signal detection strategies that include colorimetric detection, fluorescence detection, surface-enhanced Raman scattering detection, and magnetic detection, along with performance and perspectives are discussed.
- 33Di Nardo, F.; Chiarello, M.; Cavalera, S.; Baggiani, C.; Anfossi Ten, L. Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives. Sensors 2021, 21 (15), 5185, DOI: 10.3390/s2115518533https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXitl2ns7bI&md5=ea8a20e39ce32c07037b9543e2c83b60Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future PerspectivesDi Nardo, Fabio; Chiarello, Matteo; Cavalera, Simone; Baggiani, Claudio; Anfossi, LauraSensors (2021), 21 (15), 5185CODEN: SENSC9; ISSN:1424-8220. (MDPI AG)A review. The Lateral Flow Immunoassay (LFIA) is by far one of the most successful anal. platforms to perform the on-site detection of target substances. LFIA can be considered as a sort of lab-in-a-hand and, together with other point-of-need tests, has represented a paradigm shift from sample-to-lab to lab-to-sample aiming to improve decision making and turnaround time. The features of LFIAs made them a very attractive tool in clin. diagnostic where they can improve patient care by enabling more prompt diagnosis and treatment decisions. The rapidity, simplicity, relative cost-effectiveness, and the possibility to be used by nonskilled personnel contributed to the wide acceptance of LFIAs. As a consequence, from the detection of mols., organisms, and (bio)markers for clin. purposes, the LFIA application has been rapidly extended to other fields, including food and feed safety, veterinary medicine, environmental control, and many others. This review aims to provide readers with a 10-years overview of applications, outlining the trends for the main application fields and the relative compounded annual growth rates. Moreover, future perspectives and challenges are discussed.
- 34Khlebtsov, B.; Khlebtsov, N. Surface-Enhanced Raman Scattering-Based Lateral-Flow Immunoassay. Nanomaterials 2020, 10 (11), 2228, DOI: 10.3390/nano1011222834https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXpvFWjtQ%253D%253D&md5=ad91e149a34d0b6bbacebb724493e2fdSurface-enhanced Raman scattering-based lateral-flow immunoassayKhlebtsov, Boris; Khlebtsov, NikolaiNanomaterials (2020), 10 (11), 2228CODEN: NANOKO; ISSN:2079-4991. (MDPI AG)A review. Lateral flow immunoassays (LFIAs) have been developed and used in a wide range of applications, in point-of-care disease diagnoses, environmental safety, and food control. However, in its classical version, it has low sensitivity and can only perform semiquant. detection, based on colorimetric signals. Over the past decade, surface-enhanced Raman scattering (SERS) tags have been developed in order to decrease the detection limit and enable the quant. anal. of analytes. Of note, these tags needed new readout systems and signal processing algorithms, while the LFIA design remained unchanged. This review highlights SERS strategies of signal enhancement for LFIAs. The types of labels used, the possible gain in sensitivity from their use, methods of reading and processing the signal, and the prospects for use are discussed.
- 35Yadav, S.; Sadique Mohd, A.; Ranjan, P.; Kumar, N.; Singhal, A.; Srivastava, A. K.; Khan, R. SERS Based Lateral Flow Immunoassay for Point-of-Care Detection of SARS-CoV-2 in Clinical Samples. ACS Appl. Bio Mater. 2021, 4 (4), 2974– 2995, DOI: 10.1021/acsabm.1c0010235https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXmsFWhsbs%253D&md5=9052ed9f82af0653b617a56a18ae99d7SERS Based Lateral Flow Immunoassay for Point-of-Care Detection of SARS-CoV-2 in Clinical SamplesYadav, Shalu; Sadique, Mohd. Abubakar; Ranjan, Pushpesh; Kumar, Neeraj; Singhal, Ayushi; Srivastava, Avanish K.; Khan, RajuACS Applied Bio Materials (2021), 4 (4), 2974-2995CODEN: AABMCB; ISSN:2576-6422. (American Chemical Society)A review. The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role in combating the outbreak. Although conventional methods such as PCR, RT-PCR, and ELISA, etc., offer a gold-std. soln. to manage the pandemic, they cannot be implemented as a point-of-care (POC) testing arrangement. Moreover, surface-enhanced Raman spectroscopy (SERS) having a high enhancement factor provides quant. results with high specificity, sensitivity, and multiplex detection ability but lacks in POC setup. In contrast, POC devices such as lateral flow immunoassay (LFIA) offer rapid, simple-to-use, cost-effective, reliable platform. However, LFIA has limitations in quant. and sensitive analyses of SARS-CoV-2 detection. To resolve these concerns, we discuss a unique modality that is an integration of SERS with LFIA for quant. analyses of SARS-CoV-2. The miniaturization ability of SERS-based devices makes them promising in biosensor application and has the potential to make a better alternative of conventional diagnostic methods. This review also demonstrates the com. available and FDA/ICMR approved LFIA kits for on-site diagnosis of SARS-CoV-2.
- 36Langer, J.; Jimenez de Aberasturi, D.; Aizpurua, J.; Alvarez-Puebla, R. A.; Auguié, B.; Baumberg, J. J.; Bazan, G. C.; Bell, S. E. J.; Boisen, A.; Brolo, A. G.; Choo, J.; Cialla-May, D.; Deckert, V.; Fabris, L.; Faulds, K.; García de Abajo, F. J.; Goodacre, R.; Graham, D.; Haes, A. J.; Haynes, C. L.; Huck, C.; Itoh, T.; Käll, M.; Kneipp, J.; Kotov, N. A.; Kuang, H.; Le Ru, E. C.; Lee, H. K.; Li, J.-F.; Ling, X. Y.; Maier, S. A.; Mayerhöfer, T.; Moskovits, M.; Murakoshi, K.; Nam, J.-M.; Nie, S.; Ozaki, Y.; Pastoriza-Santos, I.; Perez-Juste, J.; Popp, J.; Pucci, A.; Reich, S.; Ren, B.; Schatz, G. C.; Shegai, T.; Schlücker, S.; Tay, L.-L.; Thomas, K. G.; Tian, Z.-Q.; Van Duyne, R. P.; Vo-Dinh, T.; Wang, Y.; Willets, K. A.; Xu, C.; Xu, H.; Xu, Y.; Yamamoto, Y. S.; Zhao, B.; Liz-Marzán, L. M. Present and Future of Surface-Enhanced Raman Scattering. ACS Nano 2020, 14 (1), 28– 117, DOI: 10.1021/acsnano.9b0422436https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1MXhs12jt7%252FN&md5=b9a563416413785a9548d12fcaa5d21aPresent and Future of Surface-Enhanced Raman ScatteringLanger, Judith; Jimenez de Aberasturi, Dorleta; Aizpurua, Javier; Alvarez-Puebla, Ramon A.; Auguie, Baptiste; Baumberg, Jeremy J.; Bazan, Guillermo C.; Bell, Steven E. J.; Boisen, Anja; Brolo, Alexandre G.; Choo, Jaebum; Cialla-May, Dana; Deckert, Volker; Fabris, Laura; Faulds, Karen; Garcia de Abajo, F. Javier; Goodacre, Royston; Graham, Duncan; Haes, Amanda J.; Haynes, Christy L.; Huck, Christian; Itoh, Tamitake; Kall, Mikael; Kneipp, Janina; Kotov, Nicholas A.; Kuang, Hua; Le Ru, Eric C.; Lee, Hiang Kwee; Li, Jian-Feng; Ling, Xing Yi; Maier, Stefan A.; Mayerhofer, Thomas; Moskovits, Martin; Murakoshi, Kei; Nam, Jwa-Min; Nie, Shuming; Ozaki, Yukihiro; Pastoriza-Santos, Isabel; Perez-Juste, Jorge; Popp, Juergen; Pucci, Annemarie; Reich, Stephanie; Ren, Bin; Schatz, George C.; Shegai, Timur; Schlucker, Sebastian; Tay, Li-Lin; Thomas, K. George; Tian, Zhong-Qun; Van Duyne, Richard P.; Vo-Dinh, Tuan; Wang, Yue; Willets, Katherine A.; Xu, Chuanlai; Xu, Hongxing; Xu, Yikai; Yamamoto, Yuko S.; Zhao, Bing; Liz-Marzan, Luis M.ACS Nano (2020), 14 (1), 28-117CODEN: ANCAC3; ISSN:1936-0851. (American Chemical Society)A review. The discovery of the enhancement of Raman scattering by mols. adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and anal. techniques. Significant exptl. and theor. effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technol., but addnl. efforts are still needed before it can be routinely used anal. and in com. products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technol. development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the prepn. of this article.
- 37Ali, A.; Nettey-Oppong, E. E.; Effah, E.; Yu, C. Y.; Muhammad, R.; Soomro, T. A.; Byun, K. M.; Choi, S. H. Miniaturized Raman Instruments for SERS-Based Point-of-Care Testing on Respiratory Viruses. Biosensors (Basel) 2022, 12 (8), 590, DOI: 10.3390/bios12080590There is no corresponding record for this reference.
- 38Tran, V.; Walkenfort, B.; König, M.; Salehi, M.; Schlücker, S. Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical Chemistry. Angew. Chem., Int. Ed. 2019, 58 (2), 442– 446, DOI: 10.1002/anie.20181091738https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1cXitFeisbvM&md5=48768fea253a50a2331556d0f1294322Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical ChemistryTran, Vi; Walkenfort, Bernd; Koenig, Matthias; Salehi, Mohammad; Schluecker, SebastianAngewandte Chemie, International Edition (2019), 58 (2), 442-446CODEN: ACIEF5; ISSN:1433-7851. (Wiley-VCH Verlag GmbH & Co. KGaA)The design of a portable Raman/SERS-LFA reader with line illumination using a custom-made fiber optic probe for rapid, quant., and ultrasensitive point-of-care testing (POCT) is presented. The pregnancy hormone human chorionic gonadotropin (hCG) is detectable in clin. samples within only 2-5 s down to approx. 1.6 mIU mL-1. This acquisition time is several orders of magnitude shorter than those of existing approaches requiring expensive Raman instrumentation, and the method is 15-times more sensitive than a com. available lateral flow assay (LFA) as the gold std. The SERS-LFA technol. paves the way for affordable, quant., and ultrasensitive POCT with multiplexing potential in real-world applications, ranging from clin. chem. to food and environmental anal. as well as drug and biowarfare agent testing.
- 39Sloan-Dennison, S.; O’Connor, E.; Dear, J. W.; Graham, D.; Faulds, K. Towards Quantitative Point of Care Detection Using SERS Lateral Flow Immunoassays. Anal Bioanal Chem. 2022, 414 (16), 4541– 4549, DOI: 10.1007/s00216-022-03933-839https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB38Xislymtb4%253D&md5=30fef49c6cbe8b741032a282cc29e9f6Towards quantitative point of care detection using SERS lateral flow immunoassaysSloan-Dennison, Sian; O'Connor, Emma; Dear, James W.; Graham, Duncan; Faulds, KarenAnalytical and Bioanalytical Chemistry (2022), 414 (16), 4541-4549CODEN: ABCNBP; ISSN:1618-2642. (Springer)A review. The rapid detection of biomols. in a point of care (POC) setting is very important for diagnostic purposes. A platform which can provide this, while still being low cost and simple to use, is paper-based lateral flow immunoassays (LFIA). LFIA combine immunol. and chromatog. to detect a target by forming an immunocomplex with a label which traps them in a test zone. Qual. anal. can be performed using the naked eye while quant. anal. takes place by measuring the optical signal provided by the label at the test zone. There are numerous detection methods available; however, many suffer from low sensitivity and lack of multiplexing capabilities or are poor at providing POC quant. anal. An attractive method to overcome this is to use nanoparticles coated in Raman reporters as the labeled species and to analyze test zones using surface-enhanced Raman scattering (SERS). Due to the wide variety of metal nanoparticles, Raman reporter and laser excitations that are available, SERS-based LFIA have been adapted to identify and quantify multiple targets at once. Large Raman microscopes combined with long mapping times have limited the platform to the lab; however, by transferring the anal. to portable Raman instruments, rapid and quant. measurements can be taken at the POC without any loss in sensitivity. Portable or handheld SERS-LFIA platforms can therefore be used anywhere, from modern clinics to remote and resource-poor settings. This review will present an overview of SERS-based LFIA platforms and the major recent advancements in multiplexing and portable and handheld detection with an outlook on the future of the platform.
- 40Fu, X.; Cheng, Z.; Yu, J.; Choo, P.; Chen, L.; Choo, J. A SERS-Based Lateral Flow Assay Biosensor for Highly Sensitive Detection of HIV-1 DNA. Biosens Bioelectron 2016, 78, 530– 537, DOI: 10.1016/j.bios.2015.11.09940https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2MXhvFOmurfO&md5=3fba9a9b74e9c8232dea87776e247a68A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNAFu, Xiuli; Cheng, Ziyi; Yu, Jimin; Choo, Priscilla; Chen, Lingxin; Choo, JaebumBiosensors & Bioelectronics (2016), 78 (), 530-537CODEN: BBIOE4; ISSN:0956-5663. (Elsevier B.V.)User-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quant. anal. because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems assocd. with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quant. anal. of a specific biomarker in the low concn. range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quant. analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quant. detect a broad range of genetic diseases with high sensitivity.
- 41Hwang, J.; Lee, S.; Choo, J. Application of a SERS-Based Lateral Flow Immunoassay Strip for the Rapid and Sensitive Detection of Staphylococcal Enterotoxin B. Nanoscale 2016, 8 (22), 11418– 11425, DOI: 10.1039/C5NR07243C41https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC28Xlt1GgtQ%253D%253D&md5=1ac172fc6d6f779ae283bfb54e4ba64fApplication of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin BHwang, Joonki; Lee, Sangyeop; Choo, JaebumNanoscale (2016), 8 (22), 11418-11425CODEN: NANOHL; ISSN:2040-3372. (Royal Society of Chemistry)A novel surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFA) biosensor was developed to resolve problems assocd. with conventional LFA strips (e.g., limits in quant. anal. and low sensitivity). In our SERS-based biosensor, Raman reporter-labeled hollow gold nanospheres (HGNs) were used as SERS detection probes instead of gold nanoparticles. With the proposed SERS-based LFA strip, the presence of a target antigen can be identified through a color change in the test zone. Furthermore, highly sensitive quant. evaluation is possible by measuring SERS signals from the test zone. To verify the feasibility of the SERS-based LFA strip platform, an immunoassay of staphylococcal enterotoxin B (SEB) was performed as a model reaction. The limit of detection (LOD) for SEB, as detd. with the SERS-based LFA strip, was estd. to be 0.001 ng mL-1. This value is approx. three orders of magnitude more sensitive than that achieved with the corresponding ELISA-based method. The proposed SERS-based LFA strip sensor shows significant potential for the rapid and sensitive detection of target markers in a simplified manner.
- 42Tran, V.; Walkenfort, B.; Kçnig, M.; Salehi, M.; Schlücker, S. Point-of-Care Testing Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical Chemistry. Angew. Chem. Int. Ed 2019, 58, 442– 446, DOI: 10.1002/ange.20181091742https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1cXitFeisbvM&md5=48768fea253a50a2331556d0f1294322Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical ChemistryTran, Vi; Walkenfort, Bernd; Koenig, Matthias; Salehi, Mohammad; Schluecker, SebastianAngewandte Chemie, International Edition (2019), 58 (2), 442-446CODEN: ACIEF5; ISSN:1433-7851. (Wiley-VCH Verlag GmbH & Co. KGaA)The design of a portable Raman/SERS-LFA reader with line illumination using a custom-made fiber optic probe for rapid, quant., and ultrasensitive point-of-care testing (POCT) is presented. The pregnancy hormone human chorionic gonadotropin (hCG) is detectable in clin. samples within only 2-5 s down to approx. 1.6 mIU mL-1. This acquisition time is several orders of magnitude shorter than those of existing approaches requiring expensive Raman instrumentation, and the method is 15-times more sensitive than a com. available lateral flow assay (LFA) as the gold std. The SERS-LFA technol. paves the way for affordable, quant., and ultrasensitive POCT with multiplexing potential in real-world applications, ranging from clin. chem. to food and environmental anal. as well as drug and biowarfare agent testing.
- 43Wang, Y.; Yan, B.; Chen, L. SERS Tags: Novel Optical Nanoprobes for Bioanalysis. Chem. Rev. 2013, 113 (3), 1391– 1428, DOI: 10.1021/cr300120g43https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3sXitFWh&md5=b5a595679e1b9f09774b6e8dbbd5c345SERS Tags: Novel Optical Nanoprobes for BioanalysisWang, Yunqing; Yan, Bing; Chen, LingxinChemical Reviews (Washington, DC, United States) (2013), 113 (3), 1391-1428CODEN: CHREAY; ISSN:0009-2665. (American Chemical Society)A review on most recent advances in the use of surface enhanced Raman scattering tags.
- 44Altafini, A.; Roncada, P.; Guerrini, A.; Sonfack, G. M.; Accurso, D.; Caprai, E. Development of Histamine in Fresh and Canned Tuna Steaks Stored under Different Experimental Temperature Conditions. Foods 2022, 11 (24), 4034, DOI: 10.3390/foods1124403444https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhvVWrtrg%253D&md5=0e666f6f3875c0fbfe98552439d7bb10Development of Histamine in Fresh and Canned Tuna Steaks Stored under Different Experimental Temperature ConditionsAltafini, Alberto; Roncada, Paola; Guerrini, Alessandro; Sonfack, Gaetan Minkoumba; Accurso, Damiano; Caprai, ElisabettaFoods (2022), 11 (24), 4034CODEN: FOODBV; ISSN:2304-8158. (MDPI AG)Among biogenic amines, histamine is most frequently involved in foodborne intoxication. To evaluate histamine formation in tuna, several storage conditions were reproduced. An LC-MS/MS method was used for anal. detns. Fresh tuna samples (not contaminated and grafted with tuna muscle naturally incurred with histamine at 6000 mg/kg) were stored at 4, 12, and 20 °C, and daily samples were collected for 6 days. The development of histamine was obsd. only in grafted tuna samples. At 4 °C, histamine formation progressed from 12.8 mg/kg (day 1) up to 68.2 mg/kg (day 6). At 12 °C, higher concns. developed (23.9 mg/kg on day 1 up to 2721.3 mg/kg on day 6) relative to 20 °C (from 12.0 to 1681.0 mg/kg). It was found that at 4 °C, if grafted tuna was submerged in oil, histamine formation progressed more slowly. In a naturally contaminated sample, it was obsd. that the histamine distribution was uniform, while the normal cooking process did not affect the histamine level. Furthermore, it was found that the use of histamine-contaminated equipment for food handling may result in histamine formation in food. These results confirm the importance of implementing good hygiene practices and respecting the cold chain.
- 45Dwidar, M.; Yokobayashi, Y. Development of a Histamine Aptasensor for Food Safety Monitoring. Sci. Rep 2019, 9 (1), 16659, DOI: 10.1038/s41598-019-52876-145https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB3MjoslymtQ%253D%253D&md5=d1152075c9d0aa76692fc36d84ad7cb2Development of a histamine aptasensor for food safety monitoringDwidar Mohammed; Yokobayashi Yohei; Dwidar MohammedScientific reports (2019), 9 (1), 16659 ISSN:.Histamine produced by bacteria through decarboxylation of histidine in spoiled foods such as fish is known to cause food poisoning. Therefore, accurate and facile measurement of histamine is of practical importance. Using the recently discovered RNA aptamer that specifically recognizes histamine (A1-949 aptamer), we developed an aptasensor based on the structure-switching mechanism. Specifically, the aptamer A1-949 was fluorescently labeled at the 5' end and hybridized with a short quencher DNA strand that is partially complementary to the aptamer. The quencher strand was modified with a fluorescence quencher at its 3' terminus. Displacement of the quencher strand upon histamine binding results in an increased fluorescence. After optimizing the assay condition, the enantiomeric version of the aptasensor (L-RNA and L-DNA) was synthesized which could detect the achiral analyte with identical sensitivity and improved biochemical stability. The aptasensor performance was validated by measuring fish samples spiked with known concentrations of histamine. Finally, histamine content in spoiled fish samples was measured, and the results were compared with the measurements using a commercial enzymatic assay kit.
- 46Shimoji, K.; Isono, E.; Bakke, M. Modified Enzymatic Assays for the Determination of Histamine in Fermented Foods. J. Food Prot 2020, 83 (8), 1430– 1437, DOI: 10.4315/JFP-20-08246https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXitF2qtrrN&md5=3adb30d0aa3479ac716e54f5e68c31ddModified enzymatic assays for the determination of histamine in fermented foodsShimoji, Kazuhiko; Isono, Erika; Bakke, MikioJournal of Food Protection (2020), 83 (8), 1430-1437CODEN: JFPRDR; ISSN:0362-028X. (International Association for Food Protection)Histamine is a biogenic amine, produced in spoiled fish and some fermented products, which causes a foodborne disease similar to an allergic reaction. Because regulatory levels on histamine in food have been set by many countries or organizations, a quick and accurate anal. of histamine is of great interest. An enzymic histamine detn. method on the basis of a colorimetric assay has been used to detect histamine for raw and canned tuna due to its simplicity and rapidity. However, note that some compds. in fermented foods interfere with assay results. In this study, the pretreatments and conditions of the assay for fermented foods were evaluated. Lowering the reaction temp. from 37 to 23°C was considerably effective in reducing the interference. As a result, histamine in salami and sauerkraut (≥5 to 10 mg/kg) could be detd. with a 25-fold diln., as in the manufacturer's instructions. Histamine in soy sauce (≥10 to 20 mg/L) could also be detd. with a 100-fold diln. Removing fat and protein in cheese samples by using perchloric acid with a resultant 25-fold diln. and removing polyphenol with polyvinylpolypyrrolidone for red wine with a fivefold diln. were feasible; the limits of quantification were 5 mg/kg and 1 mg/L, resp. Good recovery rates, precision repeatability, and correlations with a high-performance liq. chromatog. method were confirmed. These protocols are expected to be applicable for histamine detn. in various foods and useful for preventing histamine food poisoning.
- 47Klueber, J.; Schrama, D.; Rodrigues, P.; Dickel, H.; Kuehn, A. Fish Allergy Management: From Component-Resolved Diagnosis to Unmet Diagnostic Needs. Curr. Treat Options Allergy 2019, 6 (4), 322– 337, DOI: 10.1007/s40521-019-00235-wThere is no corresponding record for this reference.
- 48Visciano, P.; Schirone, M.; Tofalo, R.; Suzzi, G. Histamine Poisoning and Control Measures in Fish and Fishery Products. Front Microbiol 2014, 5, 5, DOI: 10.3389/fmicb.2014.00500There is no corresponding record for this reference.
- 49Kobayashi, Y.; Yang, T.; Yu, C.-T.; Ume, C.; Kubota, H.; Shimakura, K.; Shiomi, K.; Hamada-Sato, N. Quantification of Major Allergen Parvalbumin in 22 Species of Fish by SDS–PAGE. Food Chem. 2016, 194, 345– 353, DOI: 10.1016/j.foodchem.2015.08.03749https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2MXhtlentLvI&md5=8baeeb501168fc1d49dfedfa5a3c85dbQuantification of major allergen parvalbumin in 22 species of fish by SDS-PAGEKobayashi, Yukihiro; Yang, Tao; Yu, Cheng-Tao; Ume, Chiaki; Kubota, Hiroyuki; Shimakura, Kuniyoshi; Shiomi, Kazuo; Hamada-Sato, NaokoFood Chemistry (2016), 194 (), 345-353CODEN: FOCHDJ; ISSN:0308-8146. (Elsevier Ltd.)Fish is an important causative material of food allergy. Although the allergenicity of fish is considered to correlate with the content of parvalbumin, the major fish allergen, available information about the parvalbumin content in fish is limited. In this study, a simple and reliable quantification method for fish parvalbumin by SDS-PAGE was first established. Application of the SDS-PAGE method to 22 species of fish revealed a marked variation in parvalbumin content among fish. Furthermore, the parvalbumin content was found to be higher in dorsal white muscle than in ventral white muscle, in rostral part of white muscle than in caudal part of white muscle and in white muscle than in dark muscle. IgE reactivity of fish was roughly proportional to parvalbumin content. Interestingly, large-sized migratory fish, such as salmon, swordfish and tuna, were commonly very low in both parvalbumin content and IgE reactivity.
- 50Lim, D. L.-C.; Neo, K. H.; Goh, D. L.-M.; Shek, L. P.-C.; Lee, B. W. Missing Parvalbumin: Implications in Diagnostic Testing for Tuna Allergy. Journal of Allergy and Clinical Immunology 2005, 115 (4), 874– 875, DOI: 10.1016/j.jaci.2004.12.111750https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BD2MXivV2is7Y%253D&md5=95faf2dd2dc0ed19d3c92e2d9ab03c1dMissing parvalbumin: Implications in diagnostic testing for tuna allergyLim, Dawn Li-Chern; Neo, Keng Hwee; Goh, Denise Li-Meng; Shek, Lynette Pei-Chi; Lee, Bee WahJournal of Allergy and Clinical Immunology (2005), 115 (4), 874-875CODEN: JACIBY; ISSN:0091-6749. (Elsevier Inc.)This study aims at establishing the reasons for inconsistent findings regarding the presence and absence of parvalbumin, a 12-kd protein, in tuna. A fresh whole tuna, Thunnus tonggol (Bleeker) of about 400-mm fork length (FL) was skinned and cut transversely into sections along the length, beginning about 20 mm posterior to the edge of the operculum, with each section measuring about 25 mm in thickness. Parvalbumin was found in the white muscle of tuna and was absent in the red. It was also unequally distributed, with ventral white muscle contg. slightly more parvalbumin than the dorsal white muscle. The amt. of parvalbumin decreased from the rostral region to the caudal region. The findings provide an explanation for the inconsistencies in the earlier reports of parvalbumin in tuna. It is likely that different parts of the fish were sampled, and those reporting absence of parvalbumin were likely to have used a substantial portion of the red muscle. This observation is likely to be unique to fish with large portions of red muscle in sections of its torso. Although white meat of tuna is favored when eaten raw, both white and red meat of the fish are consumed in the cooked form. Therefore, these findings have significant implications when prepg. tuna fish diagnostic allergen exts. from raw material.
- 51Schrama, D.; Raposo de Magalhães, C.; Cerqueira, M.; Carrilho, R.; Revets, D.; Kuehn, A.; Engrola, S.; Rodrigues, P. M. Fish Processing and Digestion Affect Parvalbumins Detectability in Gilthead Seabream and European Seabass. Animals 2022, 12 (21), 3022, DOI: 10.3390/ani12213022There is no corresponding record for this reference.
- 52Aksun Tümerkan, E. T. Detection of Parvalbumin Fish Allergen in Canned Tuna by Real-Time PCR Driven by Tuna Species and Can-Filling Medium. Molecules 2022, 27 (17), 5674, DOI: 10.3390/molecules27175674There is no corresponding record for this reference.
- 53Taki, A. C.; Ruethers, T.; Nugraha, R.; Karnaneedi, S.; Williamson, N. A.; Nie, S.; Leeming, M. G.; Mehr, S. S.; Campbell, D. E.; Lopata, A. L. Thermostable Allergens in Canned Fish: Evaluating Risks for Fish Allergy. Allergy 2023, 78, 3221– 3234, DOI: 10.1111/all.1586453https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhvVGlt77M&md5=fcaf2186cc0c16f70a7c64a0d3eb92f3Thermostable allergens in canned fish: Evaluating risks for fish allergyTaki, Aya C.; Ruethers, Thimo; Nugraha, Roni; Karnaneedi, Shaymaviswanathan; Williamson, Nicholas A.; Nie, Shuai; Leeming, Michael G.; Mehr, Sam S.; Campbell, Dianne E.; Lopata, Andreas L.Allergy (Oxford, United Kingdom) (2023), 78 (12), 3221-3234CODEN: LLRGDY; ISSN:0105-4538. (Wiley-Blackwell)Major fish allergens, including parvalbumin (PV), are heat stable and can withstand extensive cooking processes. Thus, the management of fish allergy generally relies on complete avoidance. Fish-allergic patients may be advised to consume canned fish, as some fish-allergic individuals have reported tolerance to canned fish. However, the safety of consuming canned fish has not been evaluated with comprehensive immunol. and mol. anal. of canned fish products. We characterized the in vitro immunoreactivity of serum obtained from fish-allergic subjects to canned fish. Seventeen canned fish products (salmon n = 8; tuna n = 7; sardine n = 2) were assessed for the content and integrity of PV using allergen-specific antibodies. Subsequently, the sIgE binding of five selected products was evaluated for individual fish-allergic patients (n = 53). Finally, sIgE-binding proteins were identified by mass spectrometry. The canned fish showed a markedly reduced PV content and binding to PV-specific antibodies compared with conventionally cooked fish. However, PV and other heat-stable fish allergens, including tropomyosin and collagen, still maintained their sIgE-binding capacity. Of 53 patients, 66% showed sIgE binding to canned fish proteins. The canned sardine contained proteins bound to sIgE from 51% of patients, followed by canned salmon (43%-45%) and tuna (8%-17%). PV was the major allergen in canned salmon and sardine. Tropomyosin and/or collagen also showed sIgE binding. We showed that canned fish products may not be safe for all fish-allergic patients. Canned fish products should only be considered into the diet of individuals with fish allergy, after detailed evaluation which may include in vitro diagnostics to various heat-stable fish allergens and food challenge conducted in suitable environments.
- 54Blanco-Covián, L.; Montes-García, V.; Girard, A.; Fernández-Abedul, M. T.; Pérez-Juste, J.; Pastoriza-Santos, I.; Faulds, K.; Graham, D.; Blanco-López, M. C. Au@Ag SERRS Tags Coupled to a Lateral Flow Immunoassay for the Sensitive Detection of Pneumolysin. Nanoscale 2017, 9 (5), 2051– 2058, DOI: 10.1039/C6NR08432J54https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2sXhtFCisQ%253D%253D&md5=b59479379103d80b4279fca476a3df7fAu@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysinBlanco-Covian, Lucia; Montes-Garcia, Veronica; Girard, Alexandre; Fernandez-Abedul, M. Teresa; Perez-Juste, Jorge; Pastoriza-Santos, Isabel; Faulds, Karen; Graham, Duncan; Blanco-Lopez, M. CarmenNanoscale (2017), 9 (5), 2051-2058CODEN: NANOHL; ISSN:2040-3372. (Royal Society of Chemistry)Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labeled probe. The combination of Au@Ag core-shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL-1. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.
- 55Fernández-Lodeiro, C.; Fernández-Lodeiro, J.; Carbó-Argibay, E.; Lodeiro, C.; Pérez-Juste, J.; Pastoriza-Santos, I. The Versatility of Fe(II) in the Synthesis of Uniform Citrate-Stabilized Plasmonic Nanoparticles with Tunable Size at Room Temperature. Nano Res. 2020, 13 (9), 2351– 2355, DOI: 10.1007/s12274-020-2854-155https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3cXhsVGjtL%252FE&md5=9c57363f2ca6a5095a70a13267ef549eThe versatility of Fe (II) in the synthesis of uniform citrate-stabilized plasmonic nanoparticles with tunable size at room temperatureFernandez-Lodeiro, Carlos; Fernandez-Lodeiro, Javier; Carbo-Argibay, Enrique; Lodeiro, Carlos; Perez-Juste, Jorge; Pastoriza-Santos, IsabelNano Research (2020), 13 (9), 2351-2355CODEN: NRAEB5; ISSN:1998-0000. (Springer GmbH)A highly versatile seed-mediated approach for the synthesis of citrate-stabilized gold, silver and palladium nanoparticles (NPs) with size control is reported. The use of iron(II) as a reducing agent enables the fabrication of monodisperse NPs in a wide range of sizes (from 15 nm to at least 120 nm (90 nm for Pd)) at room temp. The citrate as capping ligand on the NPs surface facilitates its further surface modification with proteins and thiolated mols.
- 56Rodbard, D. Statistical Quality Control and Routine Data Processing for Radioimmunoassays and Immunoradiometric Assays. Clin Chem. 1974, 20 (10), 1255– 1270, DOI: 10.1093/clinchem/20.10.125556https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADyaE2MXisFGmuw%253D%253D&md5=993d52b7426cda4df8232c848f481ef2Statistical quality control and routine data processing for radioimmunoassays and immunoradiometric assaysRodbard, DavidClinical Chemistry (Washington, DC, United States) (1974), 20 (10), 1255-70CODEN: CLCHAU; ISSN:0009-9147.A review with 43 refs.
- 57Moyano, A.; Salvador, M.; Martínez-García, J. C.; Socoliuc, V.; Vékás, L.; Peddis, D.; Alvarez, M. A.; Fernández, M.; Rivas, M.; Blanco-López, M. C. Magnetic Immunochromatographic Test for Histamine Detection in Wine. Anal Bioanal Chem. 2019, 411 (25), 6615– 6624, DOI: 10.1007/s00216-019-02031-657https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC1MXhsVGqtLvJ&md5=aa80973a002119243c41e8b9a9b2b31cMagnetic immunochromatographic test for histamine detection in wineMoyano, Amanda; Salvador, Maria; Martinez-Garcia, Jose C.; Socoliuc, Vlad; Vekas, Ladislau; Peddis, Davide; Alvarez, Miguel A.; Fernandez, Maria; Rivas, Montserrat; Blanco-Lopez, M. CarmenAnalytical and Bioanalytical Chemistry (2019), 411 (25), 6615-6624CODEN: ABCNBP; ISSN:1618-2642. (Springer)Histamine, a biogenic amine, is abundant in fermented foods and beverages, notably wine. A high intake of this monoamine may produce adverse reactions in humans, which may be severe in individuals with a reduced capacity to catabolise extrinsic histamine. Thus, control of histamine concn. during wine prodn. and before distribution is advisable. Simple, rapid, point-of-use bioanal. platforms are needed because traditional methods for the detection and quantification of histamine are expensive and time-consuming. This work applies the lateral flow immunoassay technique to histamine detection. Superparamagnetic particle labels, and an inductive sensor designed to read the test line in the immunoassay, enable magnetic quantification of the mol. The system is calibrated with histamine stds. in the interval of interest for wine prodn. A com. optical strip reader is used for comparison measurements. The lateral flow system has a limit of detection of 1.2 and 1.5 mg/L for the inductive and optical readers, resp. The capability of the inductive system for histamine quantification is demonstrated for wine samples at different processing points (at the end of alc. fermn., at the end of malolactic fermn., in freshly bottled wine, and in reserve wine). The results are validated by ultra-high-performance liq. chromatog.
- 58Wang, Q.; Haughey, S. A.; Sun, Y.-M.; Eremin, S. A.; Li, Z.-F.; Liu, H.; Xu, Z.-L.; Shen, Y.-D.; Lei, H.-T. Development of a Fluorescence Polarization Immunoassay for the Detection of Melamine in Milk and Milk Powder. Anal Bioanal Chem. 2011, 399 (6), 2275– 2284, DOI: 10.1007/s00216-010-4599-258https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC3MXjtFKitQ%253D%253D&md5=060fde82fd0f3e07c38af90eb21c1a3bDevelopment of a fluorescence polarization immunoassay for the detection of melamine in milk and milk powderWang, Qiang; Haughey, Simon A.; Sun, Yuan-Ming; Eremin, Sergei A.; Li, Zhen-Feng; Liu, Hui; Xu, Zhen-Lin; Shen, Yu-Dong; Lei, Hong-TaoAnalytical and Bioanalytical Chemistry (2011), 399 (6), 2275-2284CODEN: ABCNBP; ISSN:1618-2642. (Springer)A fluorescence polarization immunoassay (FPIA) based on a polyclonal antibody was developed for the detn. of melamine in milk. To obtain an antibody with improved sensitivity and specificity, 6-hydrazinyl-1,3,5-triazine-2,4-diamine was coupled to bovine serum albumin and used as the immunogen for the rabbit immunization. Three fluorescein-labeled melamine tracers with different structures and spacer bridges were synthesized. The structural effect of the tracers on the assay characteristics was investigated. 6-(4,6-Diamino-1,3,5-triazin-2-ylamino)-N-(2-(3-(3',6'-dihydroxy-3-oxo-2,3-dihydrospiro[indene-1,9'-xanthene]-5-yl)thioureido)ethyl)hexanamide demonstrated better sensitivity than 5-(2-(4,6-diamino-1,3,5-triazin-2-yl)hydrazinecarbothioamido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid and 3-(4,6-diamino-1,3,5-triazin-2-ylthio)-N-(2-(3-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-yl)thioureido)ethyl)propanamide. The limit of detection (10% inhibition) of the FPIA was 9.3 ng mL-1 and the IC50 (50% inhibition) value was 164.7 ng mL-1. The antibody in the FPIA showed 21.2% cross-reactivity to the fly-killing insecticide cyromazine, but had no cross-reactivity to other natural structurally related compds. Recoveries, measured in spiked milk and milk powder samples, ranged from 79.4 to 119.0%. Milk samples fortified with melamine were analyzed by this method and confirmed by high-performance liq. chromatog.-mass spectrometry. Excellent recoveries and correlation with spiked levels were obsd., suggesting that this immunoassay could be applied to the screening of melamine residues in milk and milk powder after a simple diln. procedure.
- 59Guo, M.; Zhang, J.; Lv, J.; Ke, T.; Tian, J.; Miao, K.; Wang, Y.; Kong, D.; Ruan, H.; Luo, J.; Yang, M. Development of Broad-Specific Monoclonal Antibody-Based Immunoassays for Simultaneous Ochratoxin Screening in Medicinal and Edible Herbs. Food Control 2023, 148, 109626 DOI: 10.1016/j.foodcont.2023.10962659https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3sXhsFSmtb0%253D&md5=4a3dc28cfbc2225b36be7da5e89d0dbdDevelopment of broad-specific monoclonal antibody-based immunoassays for simultaneous ochratoxin screening in medicinal and edible herbsGuo, Mengyue; Jing, zhang; Lv, Jianxin; Ke, Tongwei; Tian, Jiao; Miao, Kun; Wang, Yudan; Kong, Dandan; Ruan, Haonan; Luo, Jiaoyang; Yang, MeihuaFood Control (2023), 148 (), 109626CODEN: FOOCEV; ISSN:0956-7135. (Elsevier Ltd.)The frequent occurrence of ochratoxins in food and herbs poses a considerable threat to consumer and animal health. Here, we generated a broad-specificity monoclonal antibody against three ochratoxins. Concns. for 50% inhibition (IC50) for OTA, OTB, and OTC were 0.37, 0.23, and 2.24 ng/mL, resp. In the two matrixes, the proposed indirect competitive ELISA (ic-ELISA) exhibited a limit of detection (LOD) of 1.5μg/kg for OTA and 1.0μg/kg for OTB, resp., whereas the cut-off levels of lateral flow immunoassay (LFIA) for OTA and OTB were resp. detd. to be 1 and 0.25 ng/mL. As for OTC, the LOD of ic-ELISA and cut-off concn. of LFIA were 15.5μg/kg and 8 ng/mL for milkvetch root and 37.5μg/kg and 16 ng/mL for ginger, resp. The real sample detection results by ic-ELISA and LFIA methods were validated via LC-MS/MS anal. Therefore, the developed immunoassays are useful and effective tools for high-throughput screening and rapid on-site detn. of ochratoxins in complex matrixes.
- 60Yang, F.; Xu, L.; Dias, A. C. P.; Zhang, X. A Sensitive Sandwich ELISA Using a Modified Biotin-Streptavidin Amplified System for Histamine Detection in Fish Prawn and Crab. Food Chem. 2021, 350, 129196 DOI: 10.1016/j.foodchem.2021.12919660https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BB3MXktF2qsbo%253D&md5=2ce29bc2794b9bc0d98821ea81b8be26A sensitive sandwich ELISA using a modified biotin-streptavidin amplified system for histamine detection in fish, prawn and crabYang, Fanfan; Xu, Long; Dias, Alberto C. P.; Zhang, XiaoyingFood Chemistry (2021), 350 (), 129196CODEN: FOCHDJ; ISSN:0308-8146. (Elsevier Ltd.)Histamine poisoning from seafood is a significant public health and safety concern. To detect histamine sensitively and accurately, a novel competitive sandwich immunoassay using a modified biotin-streptavidin system coupling with polylysine was developed. Using this strategy, a sandwich ELISA with an IC50 value of 112.8 ng mL-1 and a broad linear range of 11.7-1500 ng mL-1 with a correlation coeff. of 0.9942 was validated. Without any sample derivatization procedure, the recovery of histamine ranged from 80.19% to 108.3% with a coeff. of variation of 1.43-11.7% in tuna, prawn and crab. The sandwich ELISA had a detection limit of 5.86 ng mL-1, which was 15-fold lower than an indirect competitive ELISA (ic-ELISA). This simple, sensitive and accurate method can be applied to detect histamine in routine seafood samples.
- 61DeBeeR, J.; Bell, J. W.; Nolte, F.; Arcieri, J.; Correa, G. Histamine Limits by Country: A Survey and Review. J. Food Prot 2021, 84 (9), 1610– 1628, DOI: 10.4315/JFP-21-12961https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A280%3ADC%252BB2c%252FjvFSktQ%253D%253D&md5=dfa80b4c23dd8f1fffd5146d88991365Histamine Limits by Country: A Survey and ReviewDeBEER John; Bell Jon W; Nolte Fred; Arcieri Julian; Correa GersonJournal of food protection (2021), 84 (9), 1610-1628 ISSN:.Histamine is a biogenic amine and a food safety hazard, and it is the only biogenic amine regulated by statute or hazard analysis and critical control point guidance. This article reviews the regulations for histamine levels in fish in countries around the world, including maximum limits or levels and sampling procedures in different fish preparations. The maximum histamine levels, sampling plans, and fish products are listed. The country-by-country regulations for maximum histamine acceptance levels in some food products vary by a factor of 8, from 50 ppm in some countries to a maximum of 400 ppm in other countries. For similar food products, the maximum histamine levels vary by a factor of 4 (from 50 ppm to 200 ppm) in, for example, fresh tuna. The country-by-country sampling plans vary widely as well, and these, too, are covered in detail.
- 62Schulz, F.; Homolka, T.; Bastús, N. G.; Puntes, V.; Weller, H.; Vossmeyer, T. Little Adjustments Significantly Improve the Turkevich Synthesis of Gold Nanoparticles. Langmuir 2014, 30 (35), 10779– 10784, DOI: 10.1021/la503209b62https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC2cXhtlOntLvF&md5=50501206d9a841afa7243b2b24826428Little Adjustments Significantly Improve the Turkevich Synthesis of Gold NanoparticlesSchulz, Florian; Homolka, Torge; Bastus, Neus G.; Puntes, Victor; Weller, Horst; Vossmeyer, TobiasLangmuir (2014), 30 (35), 10779-10784CODEN: LANGD5; ISSN:0743-7463. (American Chemical Society)The classical and widely used Turkevich synthesis can be improved significantly by simple adjustments. The Au nanoparticles (AuNPs) produced with the optimized protocol have a much narrower size distribution (5-8% std. deviation), and their diams. can be reproduced with unrivaled little variation (<3%). Also, large vols. of these particles can be produced in one synthesis; the authors routinely synthesize 1000 mL of ∼3.5 nM AuNPs. The key features of the improved protocol are the control of the pH by using a citrate buffer instead of a citrate soln. as the reducing agent or stabilizer and optimized mixing of reagents. Further, the shape uniformity of the particles can be improved by addn. of 0.02 mM EDTA. While the proposed protocol is as straightforward as the original Turkevich protocol, it is more tolerant against variations in precursor concn.
- 63Carrera, M.; Cañas, B.; Gallardo, J. M. Rapid Direct Detection of the Major Fish Allergen, Parvalbumin, by Selected MS/MS Ion Monitoring Mass Spectrometry. J. Proteomics 2012, 75 (11), 3211– 3220, DOI: 10.1016/j.jprot.2012.03.03063https://chemport.cas.org/services/resolver?origin=ACS&resolution=options&coi=1%3ACAS%3A528%3ADC%252BC38XlvFymtrs%253D&md5=faeaacef2d1ccd790e5fe85c11b6732eRapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometryCarrera, Monica; Canas, Benito; Gallardo, Jose M.Journal of Proteomics (2012), 75 (11), 3211-3220CODEN: JPORFQ; ISSN:1874-3919. (Elsevier B.V.)Parvalbumins beta (β-PRVBs) are considered the major fish allergens. A new strategy for the rapid and direct detection of these allergens in any foodstuff is presented in this work. The proposed methodol. is based on the purifn. of β-PRVBs by treatment with heat, the use of accelerated in-soln. trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the direct detection of the presence of fish β-PRVBs in any food product in less than 2 h.
Supporting Information
Supporting Information
The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsanm.3c04696.
SERS spectra of the SERS tags and their assignments; optimization of running buffers and pHs; calibration curves; and fittings for parvalbumin and histamine (PDF)
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