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In Situ Forming Silk Sericin-Based Hydrogel: A Novel Wound Healing Biomaterial
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    Tissue Engineering and Regenerative Medicine

    In Situ Forming Silk Sericin-Based Hydrogel: A Novel Wound Healing Biomaterial
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    • Sara Baptista-Silva*
      Sara Baptista-Silva
      Universidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
      *Email: [email protected]
    • Sandra Borges
      Sandra Borges
      Universidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
    • Ana Rita Costa-Pinto
      Ana Rita Costa-Pinto
      Universidade do Porto, i3S—Instituto de Investigação e Inovação em Saúde, Porto 4200-135, Portugal
    • Raquel Costa
      Raquel Costa
      Department of Biomedicine, Faculty of Medicine, University of Porto, Porto 4099-002, Portugal
      More by Raquel Costa
    • Manuela Amorim
      Manuela Amorim
      Universidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
    • Juliana R. Dias
      Juliana R. Dias
      Centre for Rapid and Sustainable Product Development, Instituto Politécnico de Leiria, Zona Industrial, Marinha Grande 2430-028, Portugal
    • Óscar Ramos
      Óscar Ramos
      Universidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
      More by Óscar Ramos
    • Paulo Alves
      Paulo Alves
      Centre for Interdisciplinary Research in Health (CIIS)—Wounds Research Lab, Universidade Católica Portuguesa, ICS—Instituto de Ciências da Saúde, Porto 4169-005, Portugal
      More by Paulo Alves
    • Pedro Lopes Granja
      Pedro Lopes Granja
      Universidade do Porto, i3S—Instituto de Investigação e Inovação em Saúde, Porto 4200-135, Portugal
    • Raquel Soares
      Raquel Soares
      Department of Biomedicine, Faculty of Medicine, University of Porto, Porto 4099-002, Portugal
    • Manuela Pintado
      Manuela Pintado
      Universidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
    • Ana Leite Oliveira*
      Ana Leite Oliveira
      Universidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
      *Email: [email protected]
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    ACS Biomaterials Science & Engineering

    Cite this: ACS Biomater. Sci. Eng. 2021, 7, 4, 1573–1586
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    https://doi.org/10.1021/acsbiomaterials.0c01745
    Published March 17, 2021
    Copyright © 2021 American Chemical Society

    Abstract

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    In situ cross-linked hydrogels have the advantage of effectively fulfilling the wound in its shape and depth. Amongst the new generation of natural-based biopolymers being proposed for wound care and skin regeneration, silk sericin is particularly interesting due to its exceptional properties such as biocompatibility, biodegradability, and antioxidant behavior, among others. In this study, a new enzyme-mediated cross-linked hydrogel composed of silk sericin is proposed for the first time. The developed hydrogel cross-linking strategy was performed via horseradish peroxidase, under physiological conditions, and presented gelling kinetics under 3 min, as demonstrated by its rheological behavior. The hydrogels presented a high degree of transparency, mainly due to their amorphous conformation. Degradation studies revealed that the hydrogels were stable in phosphate buffer solution (PBS) (pH 7.4) for 17 days, while in the presence of protease XIV (3.5 U/mg) and under acute and chronic physiological pH values, the stability decreased to 7 and 4 days, respectively. During protease degradation, the present sericin hydrogels demonstrated antioxidant activity. In vitro studies using an L929 fibroblast cell line demonstrated that these hydrogels were noncytotoxic, promoting cell adhesion and massive cell colonization after 7 days of culture, demonstrating that cells maintained their viability and proliferation. In addition, the application of sericin-based hydrogel in an in vivo diabetic wound model validated the feasibility of the in situ methodology and demonstrated a local anti-inflammatory effect, promoting the healing process. This study presents a simple, fast, and practical in situ approach to produce a sericin-based hydrogel able to be applied in low exudative chronic wounds. Moreover, the study herein reported fosters the valorization of a textile industrial by-product by its integration in the biomedical field.

    Copyright © 2021 American Chemical Society

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    Cited By

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    This article is cited by 52 publications.

    1. Nicolas Deroose, Laurens Parmentier, Bart Devreese, Peter Dubruel. Enhanced Tunability of Photo-Cross-Linkable Silk Sericins from Bombyx mori. Biomacromolecules 2024, Article ASAP.
    2. Sneha Sabu Mathew, Hanna J. Maria, Benjamin J. Allardyce, Rangam Rajkhowa, Sabu Thomas. Waste to Wealth: Exploring the Versatile Prospects of Discarded Silk Sericin. ACS Sustainable Chemistry & Engineering 2024, 12 (3) , 1165-1184. https://doi.org/10.1021/acssuschemeng.3c04264
    3. Ziyuan Li, Fuping Lu, Yihan Liu. A Review of the Mechanism, Properties, and Applications of Hydrogels Prepared by Enzymatic Cross-linking. Journal of Agricultural and Food Chemistry 2023, 71 (27) , 10238-10249. https://doi.org/10.1021/acs.jafc.3c01162
    4. Soo in Kim, Ga Yeong Jeon, Se Eun Kim, Seung Ho Choe, Seung Jae Kim, Jin Sol Seo, Tae Woong Kang, Jeong Eun Song, Gilson Khang. Injectable Hydrogel Based on Gellan Gum/Silk Sericin for Application as a Retinal Pigment Epithelium Cell Carrier. ACS Omega 2022, 7 (45) , 41331-41340. https://doi.org/10.1021/acsomega.2c05113
    5. Vinay Kumar, Amit Kumar, Narendra Singh Chauhan, Govind Yadav, Mayank Goswami, Gopinath Packirisamy. Design and Fabrication of a Dual Protein-Based Trilayered Nanofibrous Scaffold for Efficient Wound Healing. ACS Applied Bio Materials 2022, 5 (6) , 2726-2740. https://doi.org/10.1021/acsabm.2c00200
    6. Rony Aad, Ivana Dragojlov, Simone Vesentini. Sericin Protein: Structure, Properties, and Applications. Journal of Functional Biomaterials 2024, 15 (11) , 322. https://doi.org/10.3390/jfb15110322
    7. Mohashin Kabir, Mohammed Kamrul Hasan, Mohammed Nuramurtada Rafi, Md. Reazuddin Repon, Tarikul Islam, Joykrisna Saha, Mahbubur Rahman. Biowaste Transformation to Functional Materials: Structural Properties, Extraction Methods, Applications, and Challenges of Silk Sericin. ChemistrySelect 2024, 9 (42) https://doi.org/10.1002/slct.202403777
    8. Zixuan Zhou, Zheng Chen, Chao Ji, Chenghao Wu, Jingzhu Li, Yicheng Ma, Shunxin Jin, Xiaowan Fang, Yixin Wu, Jingnan Xun, Shichu Xiao, Shige Wang, Yongjun Zheng. A dopamine-assisted antioxidative in situ-forming hydrogel with photothermal therapy for enhancing scarless burn wound healing. Chemical Engineering Journal 2024, 498 , 155389. https://doi.org/10.1016/j.cej.2024.155389
    9. Ana Borges, María Luisa Martín Calvo, Josiana A. Vaz, Ricardo C. Calhelha. Enhancing Wound Healing: A Comprehensive Review of Sericin and Chelidonium majus L. as Potential Dressings. Materials 2024, 17 (17) , 4199. https://doi.org/10.3390/ma17174199
    10. Yu Xuan Fong, Catherine Pakrath, Fathima Shana Pattar Kadavan, Tien Thanh Nguyen, Trong Quan Luu, Borislav Stoilov, Richard Bright, Manh Tuong Nguyen, Neethu Ninan, Youhong Tang, Krasimir Vasilev, Vi Khanh Truong. Antibacterial Electrospun Membrane with Hierarchical Bead-on-String Structured Fibres for Wound Infections. Nanomaterials 2024, 14 (17) , 1429. https://doi.org/10.3390/nano14171429
    11. Mohamed A. Hassan, Amal A. Basha, Mohamed Eraky, Eman Abbas, Lamia M. El-Samad. Advancements in silk fibroin and silk sericin-based biomaterial applications for cancer therapy and wound dressing formulation: A comprehensive review. International Journal of Pharmaceutics 2024, 662 , 124494. https://doi.org/10.1016/j.ijpharm.2024.124494
    12. Namitha K. Preman, Nikhitha Amin, Sandesh G. Sanjeeva, Suprith Surya, Sukesh Kumar B, Manjunath M. Shenoy, Rajesh P. Shastry, Renjith P. Johnson. Essential Oil Components Incorporated Emulsion Hydrogels for Eradicating Dermatophytosis Caused by Pathogenic Fungi Trichophyton mentagrophytes and Microsporum canis. Advanced Healthcare Materials 2024, 19 https://doi.org/10.1002/adhm.202400811
    13. Euiseok Jeong, Seungae Lee. Conversion of Biopolymer to UV‐Cross‐Linkable Conductive Ink with High Conductivity, Biocompatibility, and Biodegradability. Advanced Materials Technologies 2024, 9 (16) https://doi.org/10.1002/admt.202302163
    14. Łukasz Mazurek, Mateusz Rybka, Jan Jurak, Jakub Frankowski, Marek Konop. Silk Sericin and Its Effect on Skin Wound Healing: A State of the Art. Macromolecular Bioscience 2024, 39 https://doi.org/10.1002/mabi.202400145
    15. Anabela Veiga, Olivia Foster, David L. Kaplan, Ana Leite Oliveira. Expanding the boundaries of silk sericin biomaterials in biomedical applications. Journal of Materials Chemistry B 2024, 12 (29) , 7020-7040. https://doi.org/10.1039/D4TB00386A
    16. Zhijie Tian, Chuncao Zhao, Ting Huang, Lining Yu, Yijie Sun, Yian Tao, Yunfeng Cao, Ruofei Du, Wenhui Lin, Jia Zeng. Silkworm Cocoon: Dual Functions as a Traditional Chinese Medicine and the Raw Material of Promising Biocompatible Carriers. Pharmaceuticals 2024, 17 (7) , 817. https://doi.org/10.3390/ph17070817
    17. Anabela Veiga, Inês V. Silva, Juliana R. Dias, Nuno M. Alves, Ana L. Oliveira, Viviana P. Ribeiro. Streamlining Skin Regeneration: A Ready-To-Use Silk Bilayer Wound Dressing. Gels 2024, 10 (7) , 439. https://doi.org/10.3390/gels10070439
    18. Jian Wang, Huan Liu, Xiaolei Shi, Sumei Qin, Jingwei Liu, Qiying Lv, Jia Liu, Qilin's Li, Zheng Wang, Lin Wang. Development and Application of an Advanced Biomedical Material‐Silk Sericin. Advanced Materials 2024, 36 (23) https://doi.org/10.1002/adma.202311593
    19. Lea Sleiman, Andreea-Daniela Lazăr (Popa), Mădălina Albu-Kaya, Minodora Maria Marin, Durmuș Alpaslan Kaya, Otilia-Ruxandra Vasile, Sorina Dinescu. Development and Investigation of an Innovative 3D Biohybrid Based on Collagen and Silk Sericin Enriched with Flavonoids for Potential Wound Healing Applications. Polymers 2024, 16 (12) , 1627. https://doi.org/10.3390/polym16121627
    20. Shouying Chen, Yiren Wang, Shuilan Bao, Li Yao, Xiao Fu, Yang Yu, Hongbin Lyu, Haowen Pang, Shengmin Guo, Hongwei Zhang, Ping Zhou, Yun Zhou. Cerium oxide nanoparticles in wound care: a review of mechanisms and therapeutic applications. Frontiers in Bioengineering and Biotechnology 2024, 12 https://doi.org/10.3389/fbioe.2024.1404651
    21. Yimin Zhang, Longdi Cheng, Ruiyun Zhang, Wanwan Ma, Zhihui Qin. Effect of rheological behaviors of polyacrylonitrile grafted sericin solution on film structure and mechanical properties. International Journal of Biological Macromolecules 2024, 266 , 131102. https://doi.org/10.1016/j.ijbiomac.2024.131102
    22. Katarzyna Adamiak, Vivian A. Gaida, Jasmin Schäfer, Lina Bosse, Clara Diemer, Russel J. Reiter, Andrzej T. Slominski, Kerstin Steinbrink, Alina Sionkowska, Konrad Kleszczyński. Melatonin/Sericin Wound Healing Patches: Implications for Melanoma Therapy. International Journal of Molecular Sciences 2024, 25 (9) , 4858. https://doi.org/10.3390/ijms25094858
    23. Neha Jain, Yuvika Singh, Aynaz Nouri, Unnati Garg, Manisha Pandey. Assessment of healing capacity of glucose-responsive smart gels on the diabetic wound: A comprehensive review. Journal of Drug Delivery Science and Technology 2024, 93 , 105403. https://doi.org/10.1016/j.jddst.2024.105403
    24. Beatriz G. Bernardes, Anabela Veiga, Joana Barros, Carlos A. García-González, Ana Leite Oliveira. Sustainable Silk-Based Particulate Systems for the Controlled Release of Pharmaceuticals and Bioactive Agents in Wound Healing and Skin Regeneration. International Journal of Molecular Sciences 2024, 25 (6) , 3133. https://doi.org/10.3390/ijms25063133
    25. Charlotte E. Berry, Camille Brenac, Caroline E. Gonzalez, Carter B. Kendig, Thalia Le, Nicholas An, Michelle F. Griffin. Natural Compounds and Biomimetic Engineering to Influence Fibroblast Behavior in Wound Healing. International Journal of Molecular Sciences 2024, 25 (6) , 3274. https://doi.org/10.3390/ijms25063274
    26. Sheng-Lan Wang, Xiao-Wei Li, Wei Xu, Quan-You Yu, Shou-Min Fang. Advances of regenerated and functionalized silk biomaterials and application in skin wound healing. International Journal of Biological Macromolecules 2024, 254 , 128024. https://doi.org/10.1016/j.ijbiomac.2023.128024
    27. Eduarda P. Oliveira, F. Raquel Maia, Rui L. Reis, Joaquim M. Oliveira. Engineering enzymatic- and photo-crosslinked silk-based hydrogels for regenerative medicine. 2024, 221-239. https://doi.org/10.1016/B978-0-323-96017-5.00009-1
    28. Simone S. Silva, Rui L. Reis, Subhas C. Kundu. Trends in silk biomaterials. 2024, 9-39. https://doi.org/10.1016/B978-0-323-96017-5.00007-8
    29. Harish Bhardwaj, Sulekha Khute, Ram Sahu, Rajendra Kumar Jangde. Advanced Drug Delivery System for Management of Chronic Diabetes Wound Healing. Current Drug Targets 2023, 24 (16) , 1239-1259. https://doi.org/10.2174/0113894501260002231101080505
    30. Yiren Wang, Lei Yang, Bo Liu, Shuang Liao, Xiao Fu, Yun Zhou, Ping Zhou. Radiation Skin Injury Care in Radiotherapy for Oncology: Mechanisms, Drug Therapy and Novel Biomaterial Application Strategies. Advanced Therapeutics 2023, 6 (11) https://doi.org/10.1002/adtp.202300024
    31. T. Shalini, G. Pavithraa, R. Ajay Rakkesh, S. Balakumar. Unravelling the nature-inspired silk sericin - Calcium phosphate hybrid nanocomposites: A promising sustainable biomaterial for hard tissue regeneration applications. Surfaces and Interfaces 2023, 42 , 103396. https://doi.org/10.1016/j.surfin.2023.103396
    32. Ankur Sood, Rakesh Bhaskar, So Yeon Won, Yong Joo Seok, Anuj Kumar, Sung Soo Han. Disulfide bond-driven hyaluronic acid/sericin nanoparticles for wound-healing application. Journal of Nanostructure in Chemistry 2023, 13 (4) , 463-480. https://doi.org/10.1007/s40097-022-00505-1
    33. Yanwei Li, Susu Wang, Yurong Li, Guozheng Zhang, Tangfeng Wu, Yongkang Wei, Xinyu Cao, Hui Yan, Peisheng Liang, Zihan Yan, Yanan Guo, Yeshun Zhang. Resveratrol loaded native silk fiber-sericin hydrogel double interpenetrating bioactive wound dressing facilitates full-thickness skin wound healing. Biomedical Materials 2023, 18 (4) , 045007. https://doi.org/10.1088/1748-605X/acd318
    34. Yanwei Li, Tangfeng Wu, Guozheng Zhang, Ai Fang, Yurong Li, Susu Wang, Hui Yan, Peisheng Liang, Jialin Lian, Yeshun Zhang. A native sericin wound dressing spun directly from silkworms enhances wound healing. Colloids and Surfaces B: Biointerfaces 2023, 225 , 113228. https://doi.org/10.1016/j.colsurfb.2023.113228
    35. Pan Du, Ling Diao, Yichi Lu, Chenyang Liu, Jin Li, Yang Chen, Junfeng Chen, Guozhong Lv, Xue Chen. Heparin-based sericin hydrogel–encapsulated basic fibroblast growth factor for in vitro and in vivo skin repair. Heliyon 2023, 9 (3) , e13554. https://doi.org/10.1016/j.heliyon.2023.e13554
    36. Doudou Hu, Tiandong Li, Wen’an Liang, Yeyuan Wang, Min Feng, Jingchen Sun. Silk sericin as building blocks of bioactive materials for advanced therapeutics. Journal of Controlled Release 2023, 353 , 303-316. https://doi.org/10.1016/j.jconrel.2022.11.019
    37. Chao Yang, Liang Yao, Lei Zhang. Silk sericin-based biomaterials shine in food and pharmaceutical industries. Smart Materials in Medicine 2023, 4 , 447-459. https://doi.org/10.1016/j.smaim.2023.01.003
    38. Antara Biswas, Namrata Banerjee, Anirudh Gururaj Patil, S. Aishwarya, Sunil S. More, Kounaina Khan, Subrahmanya Padyana, J. Madhavi, Ajar Nath Yadav, H. Ravish, P.R. Manjunath, Bindia Sahu, A.V. Raghu, Farhan Zameer. Silk-based biomaterials for biomedical applications. 2023, 157-189. https://doi.org/10.1016/B978-0-323-90545-9.00002-1
    39. Andreas S. Croft, Eugenia Spessot, Promita Bhattacharjee, Yuejiao Yang, Antonella Motta, Michael Wöltje, Benjamin Gantenbein. Biomedical applications of silk and its role for intervertebral disc repair. JOR SPINE 2022, 5 (4) https://doi.org/10.1002/jsp2.1225
    40. Paulami Dam, Sevde Altuntas, Rittick Mondal, José Roberto Vega Baudrit, Ahmet Kati, Suvankar Ghorai, Abdul Sadat, Debnirmalya Gangopadhyay, Shubhajit Shaw, Octavio L. Franco, Nimet Temur, Yavuz Nuri Ertas, Amit Kumar Mandal, Ismail Ocsoy. Silk-based nano-biocomposite scaffolds for skin organogenesis. Materials Letters 2022, 327 , 133024. https://doi.org/10.1016/j.matlet.2022.133024
    41. Andreia S. Silva, Elisabete C. Costa, Sara Reis, Carina Spencer, Ricardo C. Calhelha, Sónia P. Miguel, Maximiano P. Ribeiro, Lillian Barros, Josiana A. Vaz, Paula Coutinho. Silk Sericin: A Promising Sustainable Biomaterial for Biomedical and Pharmaceutical Applications. Polymers 2022, 14 (22) , 4931. https://doi.org/10.3390/polym14224931
    42. Karen Yuanting Tang, Jerry Zhi Xiong Heng, Casandra Hui Teng Chai, Chui Yu Chan, Beverly Qian Ling Low, Serene Ming En Chong, Hong Yi Loh, Zibiao Li, Enyi Ye, Xian Jun Loh. Modified Bacterial Cellulose for Biomedical Applications. Chemistry – An Asian Journal 2022, 17 (19) https://doi.org/10.1002/asia.202200598
    43. Juin-Hong Cherng, Shu-Jen Chang, Yaw-Kwan Chiu, Yu-Hsiang Chiu, Tong-Jing Fang, Hsiang-Cheng Chen. Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration. Frontiers in Bioengineering and Biotechnology 2022, 10 https://doi.org/10.3389/fbioe.2022.925197
    44. Alap Ali Zahid, Aishik Chakraborty, Yasmeen Shamiya, Shruthi Polla Ravi, Arghya Paul. Leveraging the advancements in functional biomaterials and scaffold fabrication technologies for chronic wound healing applications. Materials Horizons 2022, 9 (7) , 1850-1865. https://doi.org/10.1039/D2MH00115B
    45. Yeshun Zhang, Wu Tangfeng, Congyi Shen, Gang Xu, Heguang Chen, Hui Yan, Meng Xiong, Guozheng Zhang. A Robust Sericin Hydrogel Formed by a Native Sericin from Silkworm Bodies. Fibers and Polymers 2022, 23 (7) , 1826-1833. https://doi.org/10.1007/s12221-022-4066-7
    46. Bhagyashree Biswal, Aritra Kumar Dan, Atanu Sengupta, Manoja Das, Birendra Kumar Bindhani, Debadutta Das, Pankaj Kumar Parhi. Extraction of Silk Fibroin with Several Sericin Removal Processes and its Importance in Tissue Engineering: A Review. Journal of Polymers and the Environment 2022, 30 (6) , 2222-2253. https://doi.org/10.1007/s10924-022-02381-w
    47. Sara Baptista-Silva, Beatriz G. Bernardes, Sandra Borges, Ilda Rodrigues, Rui Fernandes, Susana Gomes-Guerreiro, Marta Teixeira Pinto, Manuela Pintado, Raquel Soares, Raquel Costa, Ana Leite Oliveira. Exploring Silk Sericin for Diabetic Wounds: An In Situ-Forming Hydrogel to Protect against Oxidative Stress and Improve Tissue Healing and Regeneration. Biomolecules 2022, 12 (6) , 801. https://doi.org/10.3390/biom12060801
    48. Anabela Veiga, Rui Magalhães, Marta M. Duarte, Juliana R. Dias, Nuno M. Alves, Ana Rita Costa-Pinto, Filipa Castro, Fernando Rocha, Ana L. Oliveira. Continuous Production of Highly Tuned Silk/Calcium-Based Composites: Exploring New Pathways for Skin Regeneration. Molecules 2022, 27 (7) , 2249. https://doi.org/10.3390/molecules27072249
    49. Marcin Sobczak. Enzyme-Responsive Hydrogels as Potential Drug Delivery Systems—State of Knowledge and Future Prospects. International Journal of Molecular Sciences 2022, 23 (8) , 4421. https://doi.org/10.3390/ijms23084421
    50. Supamas Napavichayanun, Prompong Pienpinijtham, Narendra Reddy, Pornanong Aramwit. Superior Technique for the Production of Agarose Dressing Containing Sericin and Its Wound Healing Property. Polymers 2021, 13 (19) , 3370. https://doi.org/10.3390/polym13193370
    51. Anabela Veiga, Inês V. Silva, Marta M. Duarte, Ana L. Oliveira. Current Trends on Protein Driven Bioinks for 3D Printing. Pharmaceutics 2021, 13 (9) , 1444. https://doi.org/10.3390/pharmaceutics13091444
    52. Ionuț-Cristian Radu, Cătălin Zaharia, Ariana Hudiță, Eugenia Tanasă, Octav Ginghină, Minodora Marin, Bianca Gălățeanu, Marieta Costache. In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage. Polymers 2021, 13 (13) , 2047. https://doi.org/10.3390/polym13132047

    ACS Biomaterials Science & Engineering

    Cite this: ACS Biomater. Sci. Eng. 2021, 7, 4, 1573–1586
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsbiomaterials.0c01745
    Published March 17, 2021
    Copyright © 2021 American Chemical Society

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