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Injectable Erythrocyte Gel Loaded with Bulleyaconitine A for the Treatment of Rheumatoid Arthritis
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    Injectable Erythrocyte Gel Loaded with Bulleyaconitine A for the Treatment of Rheumatoid Arthritis
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    • Hao Chen
      Hao Chen
      Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu215004, China
      Department of Orthopedics, The Affiliated Suqian Hospital of Xuzhou Medical University, Suqian, Jiangsu223800, China
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
      More by Hao Chen
    • Bo Tian
      Bo Tian
      Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu215004, China
      More by Bo Tian
    • Xiyao Fang
      Xiyao Fang
      Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu215004, China
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    • Jinyu Bai*
      Jinyu Bai
      Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu215004, China
      *Jinyu Bai email: [email protected]
      More by Jinyu Bai
    • Qingle Ma
      Qingle Ma
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
      More by Qingle Ma
    • Yue Zhang
      Yue Zhang
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
      More by Yue Zhang
    • Jialu Xu
      Jialu Xu
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
      More by Jialu Xu
    • Beilei Wang
      Beilei Wang
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
      More by Beilei Wang
    • Qin Fan
      Qin Fan
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
      More by Qin Fan
    • Ziying Fei
      Ziying Fei
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
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    • Huaxing Dai
      Huaxing Dai
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
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    • Huajian Shan
      Huajian Shan
      Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu215004, China
      More by Huajian Shan
    • Xiang Gao
      Xiang Gao
      Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu215004, China
      More by Xiang Gao
    • Qirong Dong
      Qirong Dong
      Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu215004, China
      More by Qirong Dong
    • Chao Wang*
      Chao Wang
      Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu215123, China
      *Chao Wang email: [email protected]
      More by Chao Wang
    • Xiaozhong Zhou*
      Xiaozhong Zhou
      Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu215004, China
      *Xiaozhong Zhou email: [email protected]
    Other Access OptionsSupporting Information (1)

    ACS Biomaterials Science & Engineering

    Cite this: ACS Biomater. Sci. Eng. 2021, 7, 12, 5706–5716
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    https://doi.org/10.1021/acsbiomaterials.1c01175
    Published November 29, 2021
    Copyright © 2021 American Chemical Society

    Abstract

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    Abstract Image

    Rheumatoid arthritis (RA) is a systemic autoimmune disease with clinical manifestations including joint cartilage, synovitis, and bone damage. Here we developed an injectable erythrocyte gel loaded with Bulleyaconitine A (BLA) for the treatment of RA and demonstrated its anti-inflammatory effects in vivo and in vitro. In vitro experiments showed that BLA could effectively down-regulate the expression of pro-inflammatory factor in activated macrophages through the nuclear factor-κB (NF-κB) pathway. In vivo experiments have shown that the injection of BLA@RBCs in the inflammatory joints of CIA mice increases the local concentration of BLA in a long time. Improved therapeutic outcomes and reduced toxicity of BLA are demonstrated in our work. Together, the developed BLA@RBCs drug delivery system provides an alternative strategy to treat RA joints and shows high potential in clinical RA treatment.

    Copyright © 2021 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsbiomaterials.1c01175.

    • Primer sequences used for RT-PCR; anti-inflammatory (M2) phenotype (flow cytometry, ELISA, RT-PCR); RAW264.7 cells apoptosis assay; materials for photograph; the sustained release of RBCs-gel in vitro and in vivo; representative flow cytometric analysis of immune cells; SEM of RBCs-gel; Western blot original data (PDF)

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    Cited By

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    This article is cited by 9 publications.

    1. Yitong Chen, Yue Zhang, Beilei Wang, Qin Fan, Qianyu Yang, Jialu Xu, Huaxing Dai, Fang Xu, Chao Wang. Blood Clot Scaffold Loaded with Liposome Vaccine and siRNAs Targeting PD-L1 and TIM-3 for Effective DC Activation and Cancer Immunotherapy. ACS Nano 2023, 17 (1) , 760-774. https://doi.org/10.1021/acsnano.2c10797
    2. Ruoyu Huang, Qiuyu Pang, Lexin Zheng, Rui Duan, Ying Wang, Zhiyong Wang, Tao Wang. Acute aconitine poisoning resulting from the ingestion of medicinal liquor. Journal of Forensic Sciences 2024, 69 (6) , 2317-2324. https://doi.org/10.1111/1556-4029.15621
    3. Andrea Bezze, Carlotta Mattioda, Gianluca Ciardelli, Clara Mattu. Harnessing cells to improve transport of nanomedicines. European Journal of Pharmaceutics and Biopharmaceutics 2024, 203 , 114446. https://doi.org/10.1016/j.ejpb.2024.114446
    4. Shile Wang, Kehui Li, Yaxin Deng, Jingxin Gou, Haibing He, Tian Yin, Xing Tang, Mohamed El-Shazly, Yu Zhang. Long-acting bulleyaconitine A microspheres via intra-articular delivery for multidimensional therapy of rheumatoid arthritis. International Journal of Pharmaceutics 2024, 661 , 124414. https://doi.org/10.1016/j.ijpharm.2024.124414
    5. Ying Zhang, Hua Zhou, Xu Chen, Ningning Wang, Yunfei Zhan, Ziyi Huang, Kaiyi Ruan, Qiulan Qi, Min Deng, Yuxin Jiang. A novel tRNA-derived fragment tRF-3023b suppresses inflammation in RAW264.7 cells by targeting Cul4a through NF-κB signaling. Functional & Integrative Genomics 2024, 24 (1) https://doi.org/10.1007/s10142-024-01285-3
    6. Mirza Muhammad Faran Ashraf Baig, Lee Ki Wong, Abdul Wasy Zia, Hongkai Wu. Development of biomedical hydrogels for rheumatoid arthritis treatment. Asian Journal of Pharmaceutical Sciences 2024, 19 (1) , 100887. https://doi.org/10.1016/j.ajps.2024.100887
    7. Gelin Xiang, Nan Xing, Shaohui Wang, Yi Zhang. Antitumor effects and potential mechanisms of aconitine based on preclinical studies: an updated systematic review and meta-analysis. Frontiers in Pharmacology 2023, 14 https://doi.org/10.3389/fphar.2023.1172939
    8. Zhiqiang Luo, Lingyu Sun, Feika Bian, Yu Wang, Yunru Yu, Zhuxiao Gu, Yuanjin Zhao. Erythrocyte‐Inspired Functional Materials for Biomedical Applications. Advanced Science 2023, 10 (6) https://doi.org/10.1002/advs.202206150
    9. Aida Calderon-Rivera, Santiago Loya-Lopez, Kimberly Gomez, Rajesh Khanna. Plant and fungi derived analgesic natural products targeting voltage-gated sodium and calcium channels. Channels 2022, 16 (1) , 198-215. https://doi.org/10.1080/19336950.2022.2103234

    ACS Biomaterials Science & Engineering

    Cite this: ACS Biomater. Sci. Eng. 2021, 7, 12, 5706–5716
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsbiomaterials.1c01175
    Published November 29, 2021
    Copyright © 2021 American Chemical Society

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