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Nanofibrous Snake Venom Hemostat

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Departments of Chemistry and Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77030, United States
*E-mail: [email protected]. Tel: (713) 348-4142. Department of Chemistry, Department of Bioengineering, Rice University, Mail Stop 60, 6100 Main St., Houston, TX 77030, United States.
Cite this: ACS Biomater. Sci. Eng. 2015, 1, 12, 1300–1305
Publication Date (Web):October 22, 2015
Copyright © 2015 American Chemical Society

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    Abstract Image

    Controlling perioperative bleeding is of critical importance to minimize hemorrhaging and fatality. Patients on anticoagulant therapy such as heparin have diminished clotting potential and are at risk for hemorrhaging. Here we describe a self-assembling nanofibrous peptide hydrogel (termed SLac) that on its own can act as a physical barrier to blood loss. SLac was loaded with snake-venom derived Batroxobin (50 μg/mL) yielding a drug-loaded hydrogel (SB50). SB50 was potentiated to enhance clotting even in the presence of heparin. In vitro evaluation of fibrin and whole blood clotting helped identify appropriate concentrations for hemostasis in vivo. Batroxobin-loaded hydrogels rapidly (within 20s) stop bleeding in both normal and heparin-treated rats in a lateral liver incision model. Compared to standard of care, Gelfoam, and investigational hemostats such as Puramatrix, only SB50 showed rapid liver incision hemostasis post surgical application. This snake venom-loaded peptide hydrogel can be applied via syringe and conforms to the wound site resulting in hemostasis. This demonstrates a facile method for surgical hemostasis even in the presence of anticoagulant therapies.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsbiomaterials.5b00356

    • Video demonstrating bleeding from the new incision compared to maintenance of hemostasis in the first SB50 clotted wound: double bleed experiment (AVI)

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