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Nanofibrous Snake Venom Hemostat

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Departments of Chemistry and Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77030, United States
*E-mail: [email protected]. Tel: (713) 348-4142. Department of Chemistry, Department of Bioengineering, Rice University, Mail Stop 60, 6100 Main St., Houston, TX 77030, United States.
Cite this: ACS Biomater. Sci. Eng. 2015, 1, 12, 1300–1305
Publication Date (Web):October 22, 2015
https://doi.org/10.1021/acsbiomaterials.5b00356
Copyright © 2015 American Chemical Society

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    Abstract

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    Controlling perioperative bleeding is of critical importance to minimize hemorrhaging and fatality. Patients on anticoagulant therapy such as heparin have diminished clotting potential and are at risk for hemorrhaging. Here we describe a self-assembling nanofibrous peptide hydrogel (termed SLac) that on its own can act as a physical barrier to blood loss. SLac was loaded with snake-venom derived Batroxobin (50 μg/mL) yielding a drug-loaded hydrogel (SB50). SB50 was potentiated to enhance clotting even in the presence of heparin. In vitro evaluation of fibrin and whole blood clotting helped identify appropriate concentrations for hemostasis in vivo. Batroxobin-loaded hydrogels rapidly (within 20s) stop bleeding in both normal and heparin-treated rats in a lateral liver incision model. Compared to standard of care, Gelfoam, and investigational hemostats such as Puramatrix, only SB50 showed rapid liver incision hemostasis post surgical application. This snake venom-loaded peptide hydrogel can be applied via syringe and conforms to the wound site resulting in hemostasis. This demonstrates a facile method for surgical hemostasis even in the presence of anticoagulant therapies.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsbiomaterials.5b00356

    • Video demonstrating bleeding from the new incision compared to maintenance of hemostasis in the first SB50 clotted wound: double bleed experiment (AVI)

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    7. Biplab Sarkar, Zain Siddiqui, Peter K. Nguyen, Namita Dube, Wanyi Fu, Steven Park, Shivani Jaisinghani, Reshma Paul, Stephen D. Kozuch, Daiyong Deng, Patricia Iglesias-Montoro, Mengyan Li, David Sabatino, David S. Perlin, Wen Zhang, Jagannath Mondal, Vivek A. Kumar. Membrane-Disrupting Nanofibrous Peptide Hydrogels. ACS Biomaterials Science & Engineering 2019, 5 (9) , 4657-4670. https://doi.org/10.1021/acsbiomaterials.9b00967
    8. Amanda N. Moore and Jeffrey D. Hartgerink . Self-Assembling Multidomain Peptide Nanofibers for Delivery of Bioactive Molecules and Tissue Regeneration. Accounts of Chemical Research 2017, 50 (4) , 714-722. https://doi.org/10.1021/acs.accounts.6b00553
    9. Juan Cheng, Shibin Feng, Songling Han, Xiangjun Zhang, Yidan Chen, Xing Zhou, Ruibing Wang, Xiaohui Li, Houyuan Hu, and Jianxiang Zhang . Facile Assembly of Cost-Effective and Locally Applicable or Injectable Nanohemostats for Hemorrhage Control. ACS Nano 2016, 10 (11) , 9957-9973. https://doi.org/10.1021/acsnano.6b04124
    10. Quanshi Guo, Yihang Ding, Lisha Yu, Zongrui Tong, Zhengwei Mao. Bioactive Hydrogels with Pro‐coagulation Effect for Hemostasis †. Chinese Journal of Chemistry 2024, 42 (1) , 87-103. https://doi.org/10.1002/cjoc.202300380
    11. Jian Du, Jingzhong Wang, Tao Xu, Hai Yao, Lili Yu, Da Huang. Hemostasis Strategies and Recent Advances in Nanomaterials for Hemostasis. Molecules 2023, 28 (13) , 5264. https://doi.org/10.3390/molecules28135264
    12. Yayuan Guo, Nanqiong Cheng, Hongxiao Sun, Jianing Hou, Yuchen Zhang, Du Wang, Wei Zhang, Zhuoyue Chen. Advances in the development and optimization strategies of the hemostatic biomaterials. Frontiers in Bioengineering and Biotechnology 2023, 10 https://doi.org/10.3389/fbioe.2022.1062676
    13. Chen-Yu Zou, Qian-Jin Li, Juan-Juan Hu, Yu-Ting Song, Qing-Yi Zhang, Rong Nie, Jesse Li-Ling, Hui-Qi Xie. Design of biopolymer-based hemostatic material: Starting from molecular structures and forms. Materials Today Bio 2022, 17 , 100468. https://doi.org/10.1016/j.mtbio.2022.100468
    14. Ruonan Dong, Hualei Zhang, Baolin Guo. Emerging hemostatic materials for non-compressible hemorrhage control. National Science Review 2022, 9 (11) https://doi.org/10.1093/nsr/nwac162
    15. Celestine Hong, Bradley D. Olsen, Paula T. Hammond. A review of treatments for non-compressible torso hemorrhage (NCTH) and internal bleeding. Biomaterials 2022, 283 , 121432. https://doi.org/10.1016/j.biomaterials.2022.121432
    16. Bingxin Wu, Fanglin Du, Wenjing A, Guofeng Li, Xing Wang. Graphene-based hemostatic sponge. Chinese Chemical Letters 2022, 33 (2) , 703-713. https://doi.org/10.1016/j.cclet.2021.06.029
    17. Henry T. Beaman, Ellen Shepherd, Joshua Satalin, Sarah Blair, Harry Ramcharran, Serenella Serinelli, Lorenzo Gitto, Katheryn Shi Dong, David Fikhman, Gary Nieman, Steven G. Schauer, Mary Beth B. Monroe. Hemostatic shape memory polymer foams with improved survival in a lethal traumatic hemorrhage model. Acta Biomaterialia 2022, 137 , 112-123. https://doi.org/10.1016/j.actbio.2021.10.005
    18. Amanda Acevedo-Jake, Siyu Shi, Zain Siddiqui, Sreya Sanyal, Rebecca Schur, Simon Kaja, Alex Yuan, Vivek A. Kumar. Preclinical Efficacy of Pro- and Anti-Angiogenic Peptide Hydrogels to Treat Age-Related Macular Degeneration. Bioengineering 2021, 8 (12) , 190. https://doi.org/10.3390/bioengineering8120190
    19. Dedai Lu, Yuanyuan Jin, Xiangya Wang, Liyuan Xie, Qianqian Liu, Yamin Chen, Hui Wang, Ziqiang Lei. Heparin-like anticoagulant polypeptides with tunable activity: Synthesis, characterization, anticoagulative properties and clot solubilities in vitro. Materials Science and Engineering: C 2021, 129 , 112405. https://doi.org/10.1016/j.msec.2021.112405
    20. KaKyung Kim, Aryan Mahajan, Kamiya Patel, Shareef Syed, Amanda M. Acevedo‐Jake, Vivek A. Kumar. Materials and Cytokines in the Healing of Diabetic Foot Ulcers. Advanced Therapeutics 2021, 4 (9) https://doi.org/10.1002/adtp.202100075
    21. Yicheng Guo, Ying Wang, Xiaohong Zhao, Xue Li, Quan Wang, Wen Zhong, Kibret Mequanint, Rixing Zhan, Malcolm Xing, Gaoxing Luo. Snake extract–laden hemostatic bioadhesive gel cross-linked by visible light. Science Advances 2021, 7 (29) https://doi.org/10.1126/sciadv.abf9635
    22. , , , . Hydrogels for Drug Delivery. 2021, 90-124. https://doi.org/10.2174/9781681088235121010006
    23. Ayça Bal-Ozturk, Berivan Cecen, Meltem Avci-Adali, Seda Nur Topkaya, Emine Alarcin, Gokcen Yasayan, Yi-Chen Ethan Li, Bunyamin Bulkurcuoglu, Ali Akpek, Huseyin Avci, Kun Shi, Su Ryon Shin, Shabir Hassan. Tissue adhesives: From research to clinical translation. Nano Today 2021, 36 , 101049. https://doi.org/10.1016/j.nantod.2020.101049
    24. Henry T. Peng. Hemostatic agents for prehospital hemorrhage control: a narrative review. Military Medical Research 2020, 7 (1) https://doi.org/10.1186/s40779-020-00241-z
    25. Mustafa O. Guler, Ruslan Garifullin. Design and Synthesis of Peptides for Developing Biomaterials. 2020, 1-18. https://doi.org/10.1039/9781839161148-00001
    26. Xiaowei Wu, Zonghao Tang, Xiaoming Liao, Zhengchao Wang, Haiqing Liu. Fabrication of chitosan@calcium alginate microspheres with porous core and compact shell, and application as a quick traumatic hemostat. Carbohydrate Polymers 2020, 247 , 116669. https://doi.org/10.1016/j.carbpol.2020.116669
    27. Snehasish Ghosh, Archana Tripathi, Paramita Gayen, Rituparna Sinha Roy. Peptide-based topical agents and intravenous hemostat for rapid hemostasis. RSC Medicinal Chemistry 2020, 11 (10) , 1100-1111. https://doi.org/10.1039/D0MD00204F
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    29. Xiaotang Ma, Agnieszka Agas, Zain Siddiqui, KaKyung Kim, Patricia Iglesias-Montoro, Jagathi Kalluru, Vivek Kumar, James Haorah. Angiogenic peptide hydrogels for treatment of traumatic brain injury. Bioactive Materials 2020, 5 (1) , 124-132. https://doi.org/10.1016/j.bioactmat.2020.01.005
    30. Xiao‐Xiong Wang, Qi Liu, Jin‐Xia Sui, Seeram Ramakrishna, Miao Yu, Yu Zhou, Xing‐Yu Jiang, Yun‐Ze Long. Recent Advances in Hemostasis at the Nanoscale. Advanced Healthcare Materials 2019, 8 (23) https://doi.org/10.1002/adhm.201900823
    31. Xiao Yang, Wen Liu, Yifen Shi, Guanghui Xi, Mingshan Wang, Bin Liang, Yakai Feng, Xiangkui Ren, Changcan Shi. Peptide-immobilized starch/PEG sponge with rapid shape recovery and dual-function for both uncontrolled and noncompressible hemorrhage. Acta Biomaterialia 2019, 99 , 220-235. https://doi.org/10.1016/j.actbio.2019.08.039
    32. Ziran Zhai, Keming Xu, Leixia Mei, Can Wu, Jing Liu, Ziqi Liu, Lanxin Wan, Wenying Zhong. Co-assembled supramolecular hydrogels of cell adhesive peptide and alginate for rapid hemostasis and efficacious wound healing. Soft Matter 2019, 15 (42) , 8603-8610. https://doi.org/10.1039/C9SM01296F
    33. Isabel Gómez-Betancur, Vedanjali Gogineni, Andrea Salazar-Ospina, Francisco León. Perspective on the Therapeutics of Anti-Snake Venom. Molecules 2019, 24 (18) , 3276. https://doi.org/10.3390/molecules24183276
    34. Lulu Wang, Kehou Pan, Jing Li, Yun Li, Baohua Zhu, Yanan Wang, Chao Feng, Jichang Han. Influence of the physicochemical characteristics of diatom frustules on hemorrhage control. Biomaterials Science 2019, 7 (5) , 1833-1841. https://doi.org/10.1039/C9BM00099B
    35. Karel Petrak, Ravi Vissapragada, Siyu Shi, Zain Siddiqui, Ka Kim, Biplab Sarkar, Vivek Kumar. Challenges in Translating from Bench to Bed-Side: Pro-Angiogenic Peptides for Ischemia Treatment. Molecules 2019, 24 (7) , 1219. https://doi.org/10.3390/molecules24071219
    36. Seuk Keun Choi, Chan Wha Kim, Jong-Tak Kim, Young Seomun, Min Soo Park, Choon Ok Kim. Coagulant Effect and Tolerability of Yeast-Produced Recombinant Batroxobin in Healthy Adult Subjects. Clinical Drug Investigation 2018, 38 (9) , 829-835. https://doi.org/10.1007/s40261-018-0673-x
    37. Mary Beth Browning Monroe, Alexandra D. Easley, Katie Grant, Grace K. Fletcher, Calla Boyer, Duncan J. Maitland. Multifunctional Shape‐Memory Polymer Foams with Bio‐inspired Antimicrobials. ChemPhysChem 2018, 19 (16) , 1999-2008. https://doi.org/10.1002/cphc.201701015
    38. Gyeung Mi Seon, Mi Hee Lee, Byeong-Ju Kwon, Min Sung Kim, Min-Ah Koo, Young Seomun, Jong-Tak Kim, Tae Hee Kim, Jong-Chul Park. Recombinant batroxobin-coated nonwoven chitosan as hemostatic dressing for initial hemorrhage control. International Journal of Biological Macromolecules 2018, 113 , 757-763. https://doi.org/10.1016/j.ijbiomac.2018.03.017
    39. Meng Pan, Zonghao Tang, Jianbing Tu, Zhengchao Wang, Qinhui Chen, Rongdong Xiao, Haiqing Liu. Porous chitosan microspheres containing zinc ion for enhanced thrombosis and hemostasis. Materials Science and Engineering: C 2018, 85 , 27-36. https://doi.org/10.1016/j.msec.2017.12.015
    40. Amanda N. Moore, Tania L. Lopez Silva, Nicole C. Carrejo, Carlos A. Origel Marmolejo, I-Che Li, Jeffrey D. Hartgerink. Nanofibrous peptide hydrogel elicits angiogenesis and neurogenesis without drugs, proteins, or cells. Biomaterials 2018, 161 , 154-163. https://doi.org/10.1016/j.biomaterials.2018.01.033
    41. Yasutaka Baba, Michiyo Higashi, Kazuo Awai. A new embolic liquid agent comprised of amino acid. Minimally Invasive Therapy & Allied Technologies 2018, 27 (1) , 17-21. https://doi.org/10.1080/13645706.2017.1416409
    42. Jixiang Li, Xiaowei Wu, Yanqing Wu, Zonghao Tang, Xun Sun, Meng Pan, Yufeng Chen, Juanjuan Li, Rongdong Xiao, Zhengchao Wang, Haiqing Liu. Porous chitosan microspheres for application as quick in vitro and in vivo hemostat. Materials Science and Engineering: C 2017, 77 , 411-419. https://doi.org/10.1016/j.msec.2017.03.276
    43. Zeynep Karahaliloğlu, Murat Demirbilek, İbrahim Ulusoy, Berrak Gümüşkaya, Emir Baki Denkbaş. Active nano/microbilayer hemostatic agents for diabetic rat bleeding model. Journal of Biomedical Materials Research Part B: Applied Biomaterials 2017, 105 (6) , 1573-1585. https://doi.org/10.1002/jbm.b.33696
    44. Jiyun Lee, Jieun Park, Jia Yeom, Eun Hye Han, Young-Hee Lim. Inhibitory effect of bee venom on blood coagulation via anti-serine protease activity. Journal of Asia-Pacific Entomology 2017, 20 (2) , 599-604. https://doi.org/10.1016/j.aspen.2017.03.023
    45. Matthew J. Webber, Robert Langer. Drug delivery by supramolecular design. Chemical Society Reviews 2017, 46 (21) , 6600-6620. https://doi.org/10.1039/C7CS00391A
    46. Jordan J. Green, Jennifer H. Elisseeff. Mimicking biological functionality with polymers for biomedical applications. Nature 2016, 540 (7633) , 386-394. https://doi.org/10.1038/nature21005
    47. Weijuan Huang, Yixiang Wang, Yun Chen, Yanteng Zhao, Qiang Zhang, Xiang Zheng, Lingyun Chen, Lina Zhang. Strong and Rapidly Self‐Healing Hydrogels: Potential Hemostatic Materials. Advanced Healthcare Materials 2016, 5 (21) , 2813-2822. https://doi.org/10.1002/adhm.201600720

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