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Tobacco Mosaic Virus Functionalized Alginate Hydrogel Scaffolds for Bone Regeneration in Rats with Cranial Defect

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    Tobacco Mosaic Virus Functionalized Alginate Hydrogel Scaffolds for Bone Regeneration in Rats with Cranial Defect
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    † Department of Chemistry and Biochemistry, University of South Carolina, 631 Sumter Street, Columbia, South Carolina 29208, United States
    ‡ Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok, Thailand 10330
    § Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, 50 Ngamwongwan Road, Lat Yao, Chatuchak, Bangkok, Thailand 10903
    ∥ Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 801 Ferst Drive, Atlanta, Georgia 30332, United States
    ⊥ Medical Chronobiology Laboratory and Center for Colon Cancer Research, WJB Dorn VA Medical Center, 6439 Garners Ferry Road, Columbia, South Carolina 29209, United States
    *(A.G.) Phone: 1-404-894-9384. Fax: 1-404-894-2291. E-mail: [email protected]
    *(Q.W.) Phone: +1-803-777-8436. Fax: +1-803-777-9521. E-mail: [email protected]
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    ACS Biomaterials Science & Engineering

    Cite this: ACS Biomater. Sci. Eng. 2016, 2, 4, 606–615
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    https://doi.org/10.1021/acsbiomaterials.5b00561
    Published March 4, 2016
    Copyright © 2016 American Chemical Society
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    Abstract

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    Plant viruses have been highlighted among material research due to their well-defined structures in nanoscale, monodispersity, stability, and chemical functionalities. Each of the thousands coat protein subunits on a viral nanoparticle can be homogeneously modified, chemically and genetically, with a functional ligand leading to a high-density and spatial distribution of ligands on each particle (multivalency). Previous reports from our group have evidenced that substrates coated with Tobacco mosaic virus (TMV) and its mutant promote early osteogenesis of mesenchymal stem cells (MSCs). We then fabricated a three-dimensional (3D) biopolymeric scaffold with rod-like TMV in the form of a sponge-like hydrogel for tissue engineering purposes. The hydrogel was functionalized with the cellular recognition peptide, arginine–glycine–aspartic acid (RGD), through an incorporation of an RGD mutant of TMV (TMV-RGD). The virus-functionalized hydrogel materials were shown to aid bone differentiation of MSCs in vitro. Herein, we performed an in vivo study based on the TMV and TMV-RGD hydrogels in Sprague–Dawley rats with cranial bone defects. This report substantiated the hypothesis that TMV-functionalized hydrogel scaffolds did not cause systemic toxicity when implanted in the defect site and that the TMV-based hydrogel platform can support cell localization and can be further optimized for bone regeneration and repair.

    Copyright © 2016 American Chemical Society

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsbiomaterials.5b00561.

    • Animal postoperative care and monitoring, individual titer for each rat at different time points, MicroCT images with two- and three-dimensional constructs of bone excisions from rats with PAH, TMV-PAH, and RGD-PAH implanted, and descriptive statistics table of histology variables from trichrome stained sections according to the type of hydrogel implant (PDF)

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    ACS Biomaterials Science & Engineering

    Cite this: ACS Biomater. Sci. Eng. 2016, 2, 4, 606–615
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsbiomaterials.5b00561
    Published March 4, 2016
    Copyright © 2016 American Chemical Society
    Request reuse permissions

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