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Incorporation of Nanoalumina Improves Mechanical Properties and Osteogenesis of Hydroxyapatite Bioceramics

  • Hossein Tavassoli
    Hossein Tavassoli
    School of Metallurgy and Materials Engineering, Iran University of Science and Technology, P.O. Box 16846-13114, Tehran, Iran
    National Cell Bank of Iran, Pasteur Institute of Iran, P.O. Box 13169-43551, Tehran, Iran
    Department of Biomedical Engineering, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
    School of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia
  • Jafar Javadpour
    Jafar Javadpour
    School of Metallurgy and Materials Engineering, Iran University of Science and Technology, P.O. Box 16846-13114, Tehran, Iran
  • Mahdiar Taheri
    Mahdiar Taheri
    School of Metallurgy and Materials Engineering, Iran University of Science and Technology, P.O. Box 16846-13114, Tehran, Iran
    ANU College of Engineering & Computer Science, The Australian National University, Canberra, Australian Capital Territory 2601, Australia
  • Morteza Mehrjou
    Morteza Mehrjou
    National Cell Bank of Iran, Pasteur Institute of Iran, P.O. Box 13169-43551, Tehran, Iran
  • Newsha Koushki
    Newsha Koushki
    Department of Bioengineering, McGill University, Montreal, Quebec, Canada H3A 0C3
  • Farzin Arianpour
    Farzin Arianpour
    School of Metallurgy and Materials Engineering, Iran University of Science and Technology, P.O. Box 16846-13114, Tehran, Iran
    Research and Application Center, Kastamonu University, 37100 Kastamonu, Turkey
  • Mohammad Majidi
    Mohammad Majidi
    National Cell Bank of Iran, Pasteur Institute of Iran, P.O. Box 13169-43551, Tehran, Iran
  • Jalal Izadi-Mobarakeh
    Jalal Izadi-Mobarakeh
    Physiology and Pharmacology Department, Pasteur Institute of Iran, P.O. Box 13169-43551, Tehran, Iran
  • Babak Negahdari
    Babak Negahdari
    School of Advanced Technologies in Medicine, Department of Medical Biotechnology, Tehran University of Medical Sciences, Tehran, Iran
  • Peggy Chan
    Peggy Chan
    Department of Biomedical Engineering, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia
    More by Peggy Chan
  • Majid Ebrahimi Warkiani*
    Majid Ebrahimi Warkiani
    School of Biomedical Engineering, University of Technology Sydney, Ultimo, New South Wales 2007 Australia
    *E-mail: [email protected] (M.E.W.).
  • , and 
  • Shahin Bonakdar*
    Shahin Bonakdar
    National Cell Bank of Iran, Pasteur Institute of Iran, P.O. Box 13169-43551, Tehran, Iran
    *E-mail: [email protected] (S.B.).
Cite this: ACS Biomater. Sci. Eng. 2018, 4, 4, 1324–1336
Publication Date (Web):March 5, 2018
https://doi.org/10.1021/acsbiomaterials.7b00754
Copyright © 2018 American Chemical Society

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    Abstract

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    A handful of work focused on improving the intrinsic low mechanical properties of hydroxyapatite (HA) by various reinforcing agents. However, the big challenge regarding improving mechanical properties is maintaining bioactivity. To address this issue, we report fabrication of apatite-based composites by incorporation of alumina nanoparticles (n-Al2O3). Although numerous studies have used micron or submicron alumina for reinforcing hydroxyapatite, only few reports are available about the use of n-Al2O3. In this study, spark plasma sintering (SPS) method was utilized to develop HA-nAl2O3 dense bodies. Compared to the conventional sintering, decomposition of HA and formation of calcium aluminates phases are restricted using SPS. Moreover, n-Al2O3 acts as a bioactive agent while its conventional form is an inert bioceramics. The addition of n-Al2O3 resulted in 40% improvement in hardness along with a 110% increase in fracture toughness, while attaining nearly full dense bodies. The in vitro characterization of nanocomposite demonstrated improved bone-specific cell function markers as evidenced by cell attachment and proliferation, alkaline phosphatase activity, calcium and collagen detection and nitric oxide production. Specifically, gene expression analysis demonstrated that introduction of n-Al2O3 in HA matrix resulted in accelerated osteogenic differentiation of osteoblast and mesenchymal stem cells, as expression of Runx-2 and OSP showed 2.5 and 19.6 fold increase after 2 weeks (p < 0.05). Moreover, protein adsorption analysis showed enhanced adsorption of plasma proteins to HA-nAl2O3 sample compared to HA. These findings suggest that HA-nAl2O3 could be a prospective candidate for orthopedic applications due to its improved mechanical and osteogenic properties.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsbiomaterials.7b00754.

    • Table S1, a review of all the relevant publication about HA-Al2O3 composites from 1992 up to now, presents various approaches for synthesis, sintering, phase characterization, and mechanical and biological properties evaluation in each study, to highlight the significance of the present work compared to previous studies; Table S2, primers for real-time PCR; Figure S1, SEM images and EDAX of the starting powders and composite powder; Figure S2, SEM images of cell attachment after 4 and 72 h in different magnifications (PDF)

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