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3D Printing of Neural Tissues Derived from Human Induced Pluripotent Stem Cells Using a Fibrin-Based Bioink

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    Methods/Protocols

    3D Printing of Neural Tissues Derived from Human Induced Pluripotent Stem Cells Using a Fibrin-Based Bioink
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    • Emily Abelseth
      Emily Abelseth
      Biomedical Engineering Program, University of Victoria, 3800 Finnerty Road, Victoria, British Columbia V8W 2Y2, Canada
      More by Emily Abelseth
    • Laila Abelseth
      Laila Abelseth
      Biomedical Engineering Program, University of Victoria, 3800 Finnerty Road, Victoria, British Columbia V8W 2Y2, Canada
      More by Laila Abelseth
    • Laura De la Vega
      Laura De la Vega
      Department of Mechanical Engineering, University of Victoria, 3800 Finnerty Road, Victoria, British Columbia V8W 2Y2, Canada
      More by Laura De la Vega
    • Simon T. Beyer
      Simon T. Beyer
      Aspect Biosystems, 1781 W 75th Avenue, Vancouver, British Columbia V6P 6P2, Canada
      More by Simon T. Beyer
    • Samuel J. Wadsworth
      Samuel J. Wadsworth
      Aspect Biosystems, 1781 W 75th Avenue, Vancouver, British Columbia V6P 6P2, Canada
      More by Samuel J. Wadsworth
    • Stephanie M. Willerth*
      Stephanie M. Willerth
      Department of Mechanical Engineering  and  Division of Medical Sciences, University of Victoria, 3800 Finnerty Road, Victoria, British Columbia V8W 2Y2, Canada
      *Email: [email protected]
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      Orcidhttp://orcid.org/0000-0002-1665-7723
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    ACS Biomaterials Science & Engineering

    Cite this: ACS Biomater. Sci. Eng. 2019, 5, 1, 234–243
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    https://doi.org/10.1021/acsbiomaterials.8b01235
    Published November 26, 2018
    Copyright © 2018 American Chemical Society
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    Abstract

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    3D bioprinting offers the opportunity to automate the process of tissue engineering, which combines biomaterial scaffolds and cells to generate substitutes for diseased or damaged tissues. These bioprinting methods construct tissue replacements by positioning cells encapsulated in bioinks into specific locations in the resulting constructs. Human induced pluripotent stem cells (hiPSCs) serve as an important tool when engineering neural tissues. These cells can be expanded indefinitely and differentiated into the cell types found in the central nervous systems, including neurons. One common method for differentiating hiPSCs into neural tissue requires the formation of aggregates inside of defined diameter microwells cultured in chemically defined media. However, 3D bioprinting of such hiPSC-derived aggregates has not been previously reported in the literature, as it requires the development of specialized bioinks for supporting cell survival and differentiation into mature neural phenotypes. Here we detail methods including preparing base material components of the bioink, producing the bioink, and the steps involved in printing 3D neural tissues derived from hiPSC-derived neural aggregates using Aspect Biosystems’ novel RX1 printer and their lab-on-a-printer (LOP) technology.

    Copyright © 2018 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsbiomaterials.8b01235.

    • Movie S1, bioprinting with the RX1 printer step 1 (MPG)

    • Movie S2, bioprinting with the RX1 printer step 2 (MPG)

    • Movie S3, bioprinting with the RX1 printer step 3 (MPG)

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    74. Josie Chrenek, Rebecca Kirsch, Kali Scheck, Stephanie M. Willerth. Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead. STAR Protocols 2022, 3 (2) , 101348. https://doi.org/10.1016/j.xpro.2022.101348
    75. Nureddin Ashammakhi, Amin GhavamiNejad, Rumeysa Tutar, Annabelle Fricker, Ipsita Roy, Xanthippi Chatzistavrou, Ehsanul Hoque Apu, Kim-Lien Nguyen, Taby Ahsan, Ippokratis Pountos, Edward J. Caterson. Highlights on Advancing Frontiers in Tissue Engineering. Tissue Engineering Part B: Reviews 2022, 28 (3) , 633-664. https://doi.org/10.1089/ten.teb.2021.0012
    76. Ali Malekpour, Xiongbiao Chen. Printability and Cell Viability in Extrusion-Based Bioprinting from Experimental, Computational, and Machine Learning Views. Journal of Functional Biomaterials 2022, 13 (2) , 40. https://doi.org/10.3390/jfb13020040
    77. Fei Xu, Chloe Dawson, Makenzie Lamb, Eva Mueller, Evan Stefanek, Mohsen Akbari, Todd Hoare. Hydrogels for Tissue Engineering: Addressing Key Design Needs Toward Clinical Translation. Frontiers in Bioengineering and Biotechnology 2022, 10 https://doi.org/10.3389/fbioe.2022.849831
    78. Nicolas Germain, Melanie Dhayer, Salim Dekiouk, Philippe Marchetti. Current Advances in 3D Bioprinting for Cancer Modeling and Personalized Medicine. International Journal of Molecular Sciences 2022, 23 (7) , 3432. https://doi.org/10.3390/ijms23073432
    79. Alp Ozgun, David Lomboni, Hallie Arnott, William A. Staines, John Woulfe, Fabio Variola. Biomaterials-based strategies for in vitro neural models. Biomaterials Science 2022, 10 (5) , 1134-1165. https://doi.org/10.1039/D1BM01361K
    80. Monika Rajput, Pritiranjan Mondal, Parul Yadav, Kaushik Chatterjee. Light-based 3D bioprinting of bone tissue scaffolds with tunable mechanical properties and architecture from photocurable silk fibroin. International Journal of Biological Macromolecules 2022, 202 , 644-656. https://doi.org/10.1016/j.ijbiomac.2022.01.081
    81. Ahmed Fatimi, Oseweuba Valentine Okoro, Daria Podstawczyk, Julia Siminska-Stanny, Amin Shavandi. Natural Hydrogel-Based Bio-Inks for 3D Bioprinting in Tissue Engineering: A Review. Gels 2022, 8 (3) , 179. https://doi.org/10.3390/gels8030179
    82. Abigail Newman Frisch, Lior Debbi, Margarita Shuhmaher, Shaowei Guo, Shulamit Levenberg. Advances in vascularization and innervation of constructs for neural tissue engineering. Current Opinion in Biotechnology 2022, 73 , 188-197. https://doi.org/10.1016/j.copbio.2021.08.012
    83. David A Yefroyev, Sha Jin. Induced Pluripotent Stem Cells for Treatment of Alzheimer’s and Parkinson’s Diseases. Biomedicines 2022, 10 (2) , 208. https://doi.org/10.3390/biomedicines10020208
    84. Alen Trubelja, F. Kurtis Kasper, Mary C. Farach-Carson, Daniel A. Harrington. Bringing hydrogel-based craniofacial therapies to the clinic. Acta Biomaterialia 2022, 138 , 1-20. https://doi.org/10.1016/j.actbio.2021.10.056
    85. Zhouquan Fu, Liliang Ouyang, Runze Xu, Yang Yang, Wei Sun. Responsive biomaterials for 3D bioprinting: A review. Materials Today 2022, 52 , 112-132. https://doi.org/10.1016/j.mattod.2022.01.001
    86. Gabriele Griffanti, Rayan Fairag, Derek H. Rosenzweig, Lisbet Haglund, Showan N. Nazhat. Automated biofabrication of anisotropic dense fibrin gels accelerate osteoblastic differentiation of seeded mesenchymal stem cells. Journal of Materials Research 2021, 36 (24) , 4867-4882. https://doi.org/10.1557/s43578-021-00433-w
    87. Ruchi Sharma, Claire Benwood, Stephanie M. Willerth. Drug‐releasing Microspheres for Stem Cell Differentiation. Current Protocols 2021, 1 (12) https://doi.org/10.1002/cpz1.331
    88. Quan Li, Guangyan Qi, Xuming Liu, Jianfa Bai, Jikai Zhao, Guosheng Tang, Yu Shrike Zhang, Ruby Chen‐Tsai, Meng Zhang, Donghai Wang, Yuanyuan Zhang, Anthony Atala, Jia‐Qiang He, Xiuzhi Susan Sun. Universal Peptide Hydrogel for Scalable Physiological Formation and Bioprinting of 3D Spheroids from Human Induced Pluripotent Stem Cells. Advanced Functional Materials 2021, 31 (41) https://doi.org/10.1002/adfm.202104046
    89. Salwa, Lalit Kumar. Engrafted stem cell therapy for Alzheimer's disease: A promising treatment strategy with clinical outcome. Journal of Controlled Release 2021, 338 , 837-857. https://doi.org/10.1016/j.jconrel.2021.09.007
    90. Aleeza Zilberman, R. Chase Cornelison. Microphysiological models of the central nervous system with fluid flow. Brain Research Bulletin 2021, 174 , 72-83. https://doi.org/10.1016/j.brainresbull.2021.05.015
    91. Anabela Veiga, Inês V. Silva, Marta M. Duarte, Ana L. Oliveira. Current Trends on Protein Driven Bioinks for 3D Printing. Pharmaceutics 2021, 13 (9) , 1444. https://doi.org/10.3390/pharmaceutics13091444
    92. Melissa Cadena, Liqun Ning, Alexia King, Boeun Hwang, Linqi Jin, Vahid Serpooshan, Steven A. Sloan. 3D Bioprinting of Neural Tissues. Advanced Healthcare Materials 2021, 10 (15) https://doi.org/10.1002/adhm.202001600
    93. Laura De la Vega, Laila Abelseth, Ruchi Sharma, Juan Triviño-Paredes, Milena Restan, Stephanie M. Willerth. 3D Bioprinting Human‐Induced Pluripotent Stem Cells and Drug‐Releasing Microspheres to Produce Responsive Neural Tissues. Advanced NanoBiomed Research 2021, 1 (8) https://doi.org/10.1002/anbr.202000077
    94. Jagoda Litowczenko, Marta J. Woźniak-Budych, Katarzyna Staszak, Karolina Wieszczycka, Stefan Jurga, Bartosz Tylkowski. Milestones and current achievements in development of multifunctional bioscaffolds for medical application. Bioactive Materials 2021, 6 (8) , 2412-2438. https://doi.org/10.1016/j.bioactmat.2021.01.007
    95. Milena Restan Perez, Ruchi Sharma, Nadia Zeina Masri, Stephanie Michelle Willerth. 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink. Biomolecules 2021, 11 (8) , 1250. https://doi.org/10.3390/biom11081250
    96. Janko Kajtez, Fredrik Nilsson, Alessandro Fiorenzano, Malin Parmar, Jenny Emnéus. 3D biomaterial models of human brain disease. Neurochemistry International 2021, 147 , 105043. https://doi.org/10.1016/j.neuint.2021.105043
    97. Ruchi Sharma, Rebecca Kirsch, Karolina Papera Valente, Milena Restan Perez, Stephanie Michelle Willerth. Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications. Processes 2021, 9 (7) , 1205. https://doi.org/10.3390/pr9071205
    98. Wufei Dai, Yating Yang, Yumin Yang, Wei Liu. Material advancement in tissue-engineered nerve conduit. Nanotechnology Reviews 2021, 10 (1) , 488-503. https://doi.org/10.1515/ntrev-2021-0028
    99. Shahram Amini, Hossein Salehi, Mohsen Setayeshmehr, Masoud Ghorbani. Natural and synthetic polymeric scaffolds used in peripheral nerve tissue engineering: Advantages and disadvantages. Polymers for Advanced Technologies 2021, 32 (6) , 2267-2289. https://doi.org/10.1002/pat.5263
    100. Danielle Warren, Eva Tomaskovic-Crook, Gordon G. Wallace, Jeremy M. Crook. Engineering in vitro human neural tissue analogs by 3D bioprinting and electrostimulation. APL Bioengineering 2021, 5 (2) https://doi.org/10.1063/5.0032196
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    Cite this: ACS Biomater. Sci. Eng. 2019, 5, 1, 234–243
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