Preparation of Stepwise Adipogenesis-Mimicking ECM-Deposited PLGA–Collagen Hybrid Meshes and Their Influence on Adipogenic Differentiation of hMSCs
- Yazhou ChenYazhou ChenResearch Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 3050044, JapanDepartment of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, JapanMore by Yazhou Chen,
- Kyubae LeeKyubae LeeResearch Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 3050044, JapanDepartment of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, JapanMore by Kyubae Lee,
- Ying ChenYing ChenResearch Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 3050044, JapanDepartment of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, JapanMore by Ying Chen,
- Yingnan YangYingnan YangGraduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, JapanMore by Yingnan Yang,
- Naoki KawazoeNaoki KawazoeResearch Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 3050044, JapanMore by Naoki Kawazoe, and
- Guoping Chen*Guoping Chen*E-mail: [email protected]. Tel: +81-29-860-4496. Fax: +81-29-860-4673.Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 3050044, JapanDepartment of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, JapanMore by Guoping Chen
Abstract
Extracellular matrixes (ECMs) play a vital role in controlling cell functions because of their similarity to the in vivo microenvironment. The composition of ECMs is not constant but dynamically remolded during stem cell differentiation and tissue development. Development of three-dimensional (3D) biomimetic ECM scaffolds is desirable for investigation of ECM–cell interactions and tissue engineering applications. Here, 3D ECM scaffolds that mimicked the dynamic ECM remodeling during stepwise adipogenesis of human mesenchymal stem cells (hMSCs) were developed. A biodegradable hybrid mesh of poly-(dl-lactic-co-glycolic acid) and collagen was used as a template for cell culture. hMSCs were cultured in the hybrid mesh, and their adipogenic differentiation was controlled at early, late, and undifferentiated stages. Three types of stepwise 3D ECM hybrid scaffolds were prepared from the cultured cells after decellularization. They are mesenchymal stem cell ECM scaffold (SC-ECM scaffold), early-stage adipogenesis-mimicking ECM scaffold (EA-ECM scaffold), and late-stage adipogenesis-mimicking ECM scaffold (LA-ECM scaffold). The stepwise 3D ECM scaffolds had a different composition that was dependent on the differentiation stage of hMSCs. They also showed a different influence on the adipogenic differentiation of hMSCs. The EA-ECM scaffold promoted, while the SC-ECM and LA-ECM scaffolds inhibited the adipogenic differentiation of hMSCs.
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- Yazhou Chen, Kyubae Lee, Naoki Kawazoe, Yingnan Yang, Guoping Chen. PLGA–collagen–ECM hybrid scaffolds functionalized with biomimetic extracellular matrices secreted by mesenchymal stem cells during stepwise osteogenesis- co -adipogenesis. Journal of Materials Chemistry B 2019, 7 (45) , 7195-7206. https://doi.org/10.1039/C9TB01959F