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Real-Time Noninvasive Analysis of Biocatalytic PET Degradation

  • Ronny Frank
    Ronny Frank
    Centre for Biotechnology and Biomedicine, Molecular Biological-Biochemical Processing Technology, Leipzig University, Deutscher Platz 5, D-04103 Leipzig, Germany
    More by Ronny Frank
  • Dana Krinke
    Dana Krinke
    Centre for Biotechnology and Biomedicine, Molecular Biological-Biochemical Processing Technology, Leipzig University, Deutscher Platz 5, D-04103 Leipzig, Germany
    More by Dana Krinke
  • Christian Sonnendecker
    Christian Sonnendecker
    Institute of Analytical Chemistry, Leipzig University, Johannisallee 29, D-04103 Leipzig, Germany
  • Wolfgang Zimmermann
    Wolfgang Zimmermann
    Institute of Analytical Chemistry, Leipzig University, Johannisallee 29, D-04103 Leipzig, Germany
  • , and 
  • Heinz-Georg Jahnke*
    Heinz-Georg Jahnke
    Centre for Biotechnology and Biomedicine, Molecular Biological-Biochemical Processing Technology, Leipzig University, Deutscher Platz 5, D-04103 Leipzig, Germany
    *Email: [email protected]
Cite this: ACS Catal. 2022, 12, 1, 25–35
Publication Date (Web):December 9, 2021
https://doi.org/10.1021/acscatal.1c03963
Copyright © 2021 The Authors. Published by American Chemical Society

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    Abstract

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    The Earth has entered the Anthropocene, which is branded by ubiquitous and devastating environmental pollution from plastics such as polyethylene terephthalate (PET). Ecofriendly and at the same time economical solutions for plastic recycling and reuse are being sought more urgently now than ever. With the possibility to recover its building blocks, the hydrolysis of PET waste by its selective biodegradation with polyester hydrolases is an appealing solution. We demonstrate how changing the dielectric properties of PET films can be used to evaluate the performance of polyester hydrolases. For this purpose, a PET film separates two reaction chambers in an impedimetric setup to quantify the film thickness- and surface area-dependent change in capacitance caused by the enzyme. The derived degradation rates determined for the polyester hydrolases PHL7 and LCC were similar to those obtained by gravimetric and vertical scanning interferometry measurements. Compared to optical methods, this technique is also insensitive to changes in the solution composition. AFM and FEM simulations further supported that impedance spectroscopy is a powerful tool for the detailed analysis of the enzymatic hydrolysis process of PET films. The developed monitoring system enabled both high-temporal resolution and parallel processing suitable for the analysis of the enzymatic degradability of polyester films and the properties of the biocatalysts.

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acscatal.1c03963.

    • Geometry details of the polypropylene (PP) insert, impedance data of a fully open PP insert, capacitance data, FEM simulations, AFM images, degradation profile of R-PET, deviation of the time of the pore formation event, and influence of the solvent content on the capacitance analyzed by FEM simulation (PDF)

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    Cited By

    This article is cited by 9 publications.

    1. William Brinch-Pedersen, Malene Billeskov Keller, Robin Dorau, Bijoya Paul, Kenneth Jensen, Kim Borch, Peter Westh. Discovery and Surface Charge Engineering of Fungal Cutinases for Enhanced Activity on Poly(ethylene terephthalate). ACS Sustainable Chemistry & Engineering 2024, 12 (19) , 7329-7337. https://doi.org/10.1021/acssuschemeng.4c00060
    2. Zichen Wang, Manqi Xiang, Bingjie Huo, Jingxue Wang, Lina Yang, Wei Ma, Jianguang Qi, Yinglong Wang, Zhaoyou Zhu, Fanqing Meng. A novel ZnO/CQDs/PVDF piezoelectric system for efficiently degradation of antibiotics by using water flow energy in pipeline: Performance and mechanism. Nano Energy 2023, 107 , 108162. https://doi.org/10.1016/j.nanoen.2022.108162
    3. Marco Orlando, Gianluca Molla, Pietro Castellani, Valentina Pirillo, Vincenzo Torretta, Navarro Ferronato. Microbial Enzyme Biotechnology to Reach Plastic Waste Circularity: Current Status, Problems and Perspectives. International Journal of Molecular Sciences 2023, 24 (4) , 3877. https://doi.org/10.3390/ijms24043877
    4. Robert F. Dierkes, Alan Wypych, Pablo Pérez-García, Dominik Danso, Jennifer Chow, Wolfgang R. Streit, . An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Applied and Environmental Microbiology 2023, 89 (1) https://doi.org/10.1128/aem.01603-22
    5. Boyong Ye, Ruru Zhou, Caili Wang, Zixuan Wang, Zixin Zhong, Zhaoyin Hou. Alcoholysis of waste PLA-based plastics to methyl lactate over sulfated ZrO2/SiO2 catalyst. Applied Catalysis A: General 2023, 649 , 118936. https://doi.org/10.1016/j.apcata.2022.118936
    6. Thore Bach Thomsen, Sune W. Schubert, Cameron J. Hunt, Peter Westh, Anne S. Meyer. A new continuous assay for quantitative assessment of enzymatic degradation of poly(ethylene terephthalate) (PET). Enzyme and Microbial Technology 2023, 162 , 110142. https://doi.org/10.1016/j.enzmictec.2022.110142
    7. Rodrigo Andler, Till Tiso, Lars Blank, Christina Andreeßen, Jessica Zampolli, Vivian D’Afonseca, Camila Guajardo, Alvaro Díaz-Barrera. Current progress on the biodegradation of synthetic plastics: from fundamentals to biotechnological applications. Reviews in Environmental Science and Bio/Technology 2022, 21 (4) , 829-850. https://doi.org/10.1007/s11157-022-09631-2
    8. Holger Lippold, Laura Kahle, Christian Sonnendecker, Jörg Matysik, Cornelius Fischer. Temporal and spatial evolution of enzymatic degradation of amorphous PET plastics. npj Materials Degradation 2022, 6 (1) https://doi.org/10.1038/s41529-022-00305-6
    9. Anjima James, Susmita De. Cation–π and hydrophobic interaction controlled PET recognition in double mutated cutinase – identification of a novel binding subsite for better catalytic activity. RSC Advances 2022, 12 (32) , 20563-20577. https://doi.org/10.1039/D2RA03394A