In Vitro Reconstitution of a Bacterial Ergothioneine Sulfonate Catabolic PathwayClick to copy article linkArticle link copied!
- Qiongxiang YanQiongxiang YanGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Ecological Science, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaMore by Qiongxiang Yan
- Hua Huang*Hua Huang*Email: [email protected]Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Ecological Science, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaMore by Hua Huang
- Xinshuai Zhang*Xinshuai Zhang*Email: [email protected]Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Ecological Science, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaMore by Xinshuai Zhang
Abstract
Naturally occurring l-ergothioneine is a ubiquitous histidine-derived sulfur metabolite produced by bacteria and fungi. Plants acquire ergothioneine from their soil environment, whereas animals accumulate it through dietary intake. It is widely believed that ergothioneine functions as a protective antioxidant to eradicate reactive oxygen and nitrogen species. Ergothioneine oxidation leads to the metabolite ergothioneine sulfonate. Using a “genomic enzymology” strategy, we have determined the chemical steps for the ergothioneine sulfonate catabolic pathway, which involves a previously unknown reductive desulfonation reaction catalyzed by members of ornithine cyclodeaminase superfamily. In vitro reconstitution of the full pathway was demonstrated using the cloned and purified pathway enzymes fromAgrobacterium radiobacter K84. Through the identification of pathway gene homologues, the biological range of the ergothioneine sulfonate pathway was found to include α-,γ-proteobacterial, and actinobacterial species.
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This article is cited by 8 publications.
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