ACS Publications. Most Trusted. Most Cited. Most Read
Three-Dimensional Sulfite Oxidase Bioanodes Based on Graphene Functionalized Carbon Paper for Sulfite/O2 Biofuel Cells

OR SEARCH CITATIONS

My Activity
Publications

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:ACS Catal. 2019, 9, 7, 6543-6554
    Research Article

    Three-Dimensional Sulfite Oxidase Bioanodes Based on Graphene Functionalized Carbon Paper for Sulfite/O2 Biofuel Cells
    Click to copy article linkArticle link copied!

    Other Access OptionsSupporting Information (1)

    ACS Catalysis

    Cite this: ACS Catal. 2019, 9, 7, 6543–6554
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acscatal.9b01715
    Published June 11, 2019
    Copyright © 2019 American Chemical Society
    Request reuse permissions

    Abstract

    Click to copy section linkSection link copied!
    Abstract Image

    We have developed a three-dimensional (3D) graphene electrode suitable for the immobilization of human sulfite oxidase (hSO), which catalyzes the electrochemical oxidation of sulfite via direct electron transfer (DET). The electrode is fabricated by drop-casting graphene-polyethylenimine (G-P) composites on carbon papers (CPs) precoated with graphene oxide (GO). The negatively charged hSO can be adsorbed electrostatically on the positively charged matrix (G-P) on CP electrodes coated with GO (CPG), with a proper orientation for accelerated DET. Notably, further electrochemical reduction of G-P on CPG electrodes leads to a 9-fold increase of the saturation catalytic current density (jm) for sulfite oxidation reaching 24.4 ± 0.3 μA cm–2, the highest value among reported DET-based hSO bioelectrodes. The increased electron transfer rate plays a dominating role in the enhancement of direct enzymatic current because of the improved electric contact of hSO with the electrode. The optimized hSO bioelectrode shows a significant catalytic rate (kcat: 25.6 ± 0.3 s–1) and efficiency (kcat/Km: 0.231 ± 0.003 s–1 μM–1) compared to the reported hSO bioelectrodes. The assembly of the hSO bioanode and a commercial platinum biocathode allows the construction of sulfite/O2 enzymatic biofuel cells (EBFCs) with flowing fuels. The optimized EBFC displays an open-circuit voltage (OCV) of 0.64 ± 0.01 V and a maximum power density of 61 ± 6 μW cm–2 (122 ± 12 mW m–3) at 30 °C, which exceeds the best reported value by more than 6 times.

    Copyright © 2019 American Chemical Society

    Keywords

    what are keywords

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Supporting Information

    Click to copy section linkSection link copied!

    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acscatal.9b01715.

    • Additional data and figures including preparation of hSO bioelectrodes, EBFC assembly, AFM image, XPS and UV–vis spectra, and electrochemical data, summary of relative peak area percentage of carbon bindings, ζ potentials, key voltammetry parameters, EIS fitting parameters, ECSAs, comparison of ECSA and Rct, bioelectrode activity assay, and enzyme kinetic parameters (PDF)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    Click to copy section linkSection link copied!
    Citation Statements
    Explore this article's citation statements on scite.ai
    powered by  Scite

    This article is cited by 39 publications.

    1. Yuetong Kang, Yun Zhang, Xinrui Li, Xiaoyu Wang, Jingqi Zhang, Lidong Li. Protein-Assisted Molybdenum Disulfide as Biomimetic Nanozyme for Antibacterial Application. ACS Applied Bio Materials 2023, 6 (7) , 2898-2904. https://doi.org/10.1021/acsabm.3c00341
    2. Phan Gia Le, Qilu Wu, Do Yun Kong, Jun Ge, Moon Il Kim. Tailoring Nanostructured Supports to Achieve High Performance in Enzymatic Biofuel Cells. ACS Applied Energy Materials 2022, 5 (11) , 13113-13127. https://doi.org/10.1021/acsaem.2c02426
    3. Zilin Chen, Yao Yao, Tian Lv, Yunlong Yang, Yanan Liu, Tao Chen. Flexible and Stretchable Enzymatic Biofuel Cell with High Performance Enabled by Textile Electrodes and Polymer Hydrogel Electrolyte. Nano Letters 2022, 22 (1) , 196-202. https://doi.org/10.1021/acs.nanolett.1c03621
    4. Tajiguli Yimamumaimaiti, Xuanzhao Lu, Jian-Rong Zhang, Linlin Wang, Jun-Jie Zhu. Efficient Blood-toleration Enzymatic Biofuel Cell via In Situ Protection of an Enzyme Catalyst. ACS Applied Materials & Interfaces 2020, 12 (37) , 41429-41436. https://doi.org/10.1021/acsami.0c11186
    5. Yuan Chen, Xiaoju Wu, Tongming Chen, Guowei Yang. Hot Carriers and Photothermal Effects of Monolayer MoOx for Promoting Sulfite Oxidase Mimetic Activity. ACS Applied Materials & Interfaces 2020, 12 (17) , 19357-19368. https://doi.org/10.1021/acsami.0c04987
    6. Ling Chen, Mayowa Oyebanji, Haizhu Yu, Manzhou Zhu. A mini-review on oxidoreductase-mimicking nanomaterials. Emergent Materials 2025, 8 (1) , 85-111. https://doi.org/10.1007/s42247-024-00939-z
    7. Henrik Bohr, Irene Shim, Jens Ulstrup, Xinxin Xiao. Protein Quakes in Redox Metalloenzymes: Clues to Molecular Enzyme Conductivity Triggered by Binding of Small Substrate Molecules. ChemistryOpen 2024, 13 (12) https://doi.org/10.1002/open.202400190
    8. Xin Zhou, Yang Liu, Chuanshu He, Yuqi Zhang, Yujia Xiang, Jialong Yin, Shuai Yang, Zirong Li, Yue Yuan, Peng Zhou, Yi Ren, Heng Zhang, Bo Lai. Influence of S(IV) reduction on the reaction mechanisms and the generation of reactive oxygen species in B-Cu@Fe/PPS system. Journal of Hazardous Materials 2024, 480 , 136441. https://doi.org/10.1016/j.jhazmat.2024.136441
    9. Sabeela Beevi Ummalyma, Thallada Bhaskar. Recent advances in the role of biocatalyst in biofuel cells and its application: An overview. Biotechnology and Genetic Engineering Reviews 2024, 40 (3) , 2051-2089. https://doi.org/10.1080/02648725.2023.2197715
    10. Indrani Chakraborty, Richard T. Olsson, Richard L. Andersson, Annu Pandey. Glucose-based biofuel cells and their applications in medical implants: A review. Heliyon 2024, 10 (13) , e33615. https://doi.org/10.1016/j.heliyon.2024.e33615
    11. Peng Yu, Juan Tan, Zhiguo Wang, Chun Zhang, Qiongchao Wang, Keming Zhu, Chao Peng, Xinxin Xiao, Wei Huang. Enhanced electron transfer pathway of zero-valent iron particles immobilized on coconut shell derived carbon for prolonged Cr(VI) removal. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2024, 682 , 132863. https://doi.org/10.1016/j.colsurfa.2023.132863
    12. Jingsheng Cai, Fei Shen, Jianqing Zhao, Xinxin Xiao. Enzymatic biofuel cell: A potential power source for self-sustained smart textiles. iScience 2024, 27 (2) , 108998. https://doi.org/10.1016/j.isci.2024.108998
    13. Iftikhar Ali, Marzia Dulal, Nazmul Karim, Shaila Afroj. 2D Material‐Based Wearable Energy Harvesting Textiles: A Review. Small Structures 2024, 5 (1) https://doi.org/10.1002/sstr.202300282
    14. Atif Khurshid Wani, Anjli Anjli, Ira Tripathi, Tahir ul Gani Mir. Interaction of food and packaging. 2024, 289-318. https://doi.org/10.1016/B978-0-443-15388-4.00011-0
    15. Sascha Morlock, Senthil Kumar Subramanian, Athina Zouni, Fred Lisdat. Bio-inorganic hybrid structures for direct electron transfer to photosystem I in photobioelectrodes. Biosensors and Bioelectronics 2022, 214 , 114495. https://doi.org/10.1016/j.bios.2022.114495
    16. Xiaomei Yan, Jing Tang, Su Ma, David Tanner, Roland Ludwig, Jens Ulstrup, Xinxin Xiao. Engineering bio-interfaces for the direct electron transfer of Myriococcum thermophilum cellobiose dehydrogenase: Towards a mediator-less biosupercapacitor/biofuel cell hybrid. Biosensors and Bioelectronics 2022, 210 , 114337. https://doi.org/10.1016/j.bios.2022.114337
    17. Xinxin Xiao, Xiaomei Yan, Jens Ulstrup. Enhancement of bioelectrochemical dioxygen reduction with oxygen-enriching materials. Current Opinion in Electrochemistry 2022, 34 , 100966. https://doi.org/10.1016/j.coelec.2022.100966
    18. Sanath Kondaveeti, Gi Dae Park, Ramasamy Shanmugam, Raviteja Pagolu, Sanjay K.S. Patel, Aarti Bisht, Dong Rip Kim, Yun Chan Kang, Jung-Kul Lee. Investigating the role of metals loaded on nitrogen-doped carbon-nanotube electrodes in electroenzymatic alcohol dehydrogenation. Applied Catalysis B: Environmental 2022, 307 , 121195. https://doi.org/10.1016/j.apcatb.2022.121195
    19. Biplab K Maiti. Cross‐talk Between (Hydrogen)Sulfite and Metalloproteins: Impact on Human Health. Chemistry – A European Journal 2022, 28 (23) https://doi.org/10.1002/chem.202104342
    20. Chengcheng Gu, Panpan Gai, Feng Li. Construction of biofuel cells-based self-powered biosensors via design of nanocatalytic system. Nano Energy 2022, 93 , 106806. https://doi.org/10.1016/j.nanoen.2021.106806
    21. Wei Huang, Chao Peng, Jing Tang, Fangyuan Diao, Murat Nulati Yesibolati, Hongyu Sun, Christian Engelbrekt, Jingdong Zhang, Xinxin Xiao, Kristian S. Mølhave. Electronic structure modulation with ultrafine Fe3O4 nanoparticles on 2D Ni-based metal-organic framework layers for enhanced oxygen evolution reaction. Journal of Energy Chemistry 2022, 65 , 78-88. https://doi.org/10.1016/j.jechem.2021.05.030
    22. Xinxin Xiao. The direct use of enzymatic biofuel cells as functional bioelectronics. eScience 2022, 2 (1) , 1-9. https://doi.org/10.1016/j.esci.2021.12.005
    23. Yuchen Hui, Huixin Wang, Wei Zuo, Xiaoyan Ma. Spider nest shaped multi-scale three-dimensional enzymatic electrodes for glucose/oxygen biofuel cells. International Journal of Hydrogen Energy 2022, 47 (9) , 6187-6199. https://doi.org/10.1016/j.ijhydene.2021.11.210
    24. Xiaomei Yan, Su Ma, Jing Tang, David Tanner, Jens Ulstrup, Xinxin Xiao, Jingdong Zhang. Direct electron transfer of fructose dehydrogenase immobilized on thiol-gold electrodes. Electrochimica Acta 2021, 392 , 138946. https://doi.org/10.1016/j.electacta.2021.138946
    25. M. Wu, K. Yao, D. Li, X. Huang, Y. Liu, L. Wang, E. Song, J. Yu, X. Yu. Self-powered skin electronics for energy harvesting and healthcare monitoring. Materials Today Energy 2021, 21 , 100786. https://doi.org/10.1016/j.mtener.2021.100786
    26. Wei Huang, Jing Tang, Fangyuan Diao, Shuo Li, Hongyu Sun, Xinxin Xiao. CoP Nanoparticles Fabricated Through the Nanoscale Kirkendall Effect Immobilized in 3D Hollow Carbon Frameworks for Oxygen Evolution Reaction. Journal of The Electrochemical Society 2021, 168 (9) , 094501. https://doi.org/10.1149/1945-7111/ac2090
    27. Linlin Wang, Xiaoge Wu, B. S. Qi‐wen Su, Rongbin Song, Jian-Rong Zhang, Jun-Jie Zhu. Enzymatic Biofuel Cell: Opportunities and Intrinsic Challenges in Futuristic Applications. Advanced Energy and Sustainability Research 2021, 2 (8) https://doi.org/10.1002/aesr.202100031
    28. Phan Gia Le, Moon Il Kim. Research Progress and Prospects of Nanozyme-Based Glucose Biofuel Cells. Nanomaterials 2021, 11 (8) , 2116. https://doi.org/10.3390/nano11082116
    29. Nabila A. Karim, Hsiharng Yang. Mini-Review: Recent Technologies of Electrode and System in the Enzymatic Biofuel Cell (EBFC). Applied Sciences 2021, 11 (11) , 5197. https://doi.org/10.3390/app11115197
    30. Aysu Yarman, Sevinc Kurbanoglu, Cem Erkmen, Bengi Uslu, Frieder W. Scheller. Quantum dot-based electrochemical molecularly imprinted polymer sensors: potentials and challenges. 2021, 121-153. https://doi.org/10.1016/B978-0-12-821670-5.00013-0
    31. Xiaomei Yan, Jing Tang, David Tanner, Jens Ulstrup, Xinxin Xiao. Direct Electrochemical Enzyme Electron Transfer on Electrodes Modified by Self-Assembled Molecular Monolayers. Catalysts 2020, 10 (12) , 1458. https://doi.org/10.3390/catal10121458
    32. Jing Tang, Xiaomei Yan, Wei Huang, Christian Engelbrekt, Jens Øllgaard Duus, Jens Ulstrup, Xinxin Xiao, Jingdong Zhang. Bilirubin oxidase oriented on novel type three-dimensional biocathodes with reduced graphene aggregation for biocathode. Biosensors and Bioelectronics 2020, 167 , 112500. https://doi.org/10.1016/j.bios.2020.112500
    33. Jing Tang, Xiaomei Yan, Christian Engelbrekt, Jens Ulstrup, Edmond Magner, Xinxin Xiao, Jingdong Zhang. Development of graphene-based enzymatic biofuel cells: A minireview. Bioelectrochemistry 2020, 134 , 107537. https://doi.org/10.1016/j.bioelechem.2020.107537
    34. Morten M. C. H. van Schie, Alexander T. Kaczmarek, Florian Tieves, Patricia Gomez de Santos, Caroline E. Paul, Isabel W. C. E. Arends, Miguel Alcalde, Günter Schwarz, Frank Hollmann. Selective Oxyfunctionalisation Reactions Driven by Sulfite Oxidase‐Catalysed In Situ Generation of H 2 O 2. ChemCatChem 2020, 12 (12) , 3186-3189. https://doi.org/10.1002/cctc.201902297
    35. Panpan Gai, Chengcheng Gu, Xinke Kong, Feng Li. Anode-Driven Controlled Release of Cathodic Fuel via pH Response for Smart Enzymatic Biofuel Cell. iScience 2020, 23 (6) , 101133. https://doi.org/10.1016/j.isci.2020.101133
    36. Aurelia Magdalena Pisoschi, Aneta Pop, Iuliana Gajaila, Florin Iordache, Razvan Dobre, Iuliana Cazimir, Andreea Iren Serban. Analytical methods applied to the assay of sulfur-containing preserving agents. Microchemical Journal 2020, 155 , 104681. https://doi.org/10.1016/j.microc.2020.104681
    37. Nicolas Mano. Recent advances in high surface area electrodes for bioelectrochemical applications. Current Opinion in Electrochemistry 2020, 19 , 8-13. https://doi.org/10.1016/j.coelec.2019.09.003
    38. Ievgen Mazurenko, Vivek Pratap Hitaishi, Elisabeth Lojou. Recent advances in surface chemistry of electrodes to promote direct enzymatic bioelectrocatalysis. Current Opinion in Electrochemistry 2020, 19 , 113-121. https://doi.org/10.1016/j.coelec.2019.11.004
    39. Rebecka Maria Larsen Werchmeister, Jing Tang, Xinxin Xiao, Ulla Wollenberger, Hans Aage Hjuler, Jens Ulstrup, Jingdong Zhang. Three-Dimensional Bioelectrodes Utilizing Graphene Based Bioink. Journal of The Electrochemical Society 2019, 166 (16) , G170-G177. https://doi.org/10.1149/2.0261916jes
    Download PDF
    Go to ACS Catalysis
    Get e-Alerts
    Get e-Alerts

    ACS Catalysis

    Cite this: ACS Catal. 2019, 9, 7, 6543–6554
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acscatal.9b01715
    Published June 11, 2019
    Copyright © 2019 American Chemical Society
    Request reuse permissions

    Article Views

    1460

    Altmetric

    -

    Citations

    39
    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.