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Multifunctionalization of Cells with a Self-Assembling Molecule to Enhance Cell Engraftment

Cite this: ACS Chem. Biol. 2019, 14, 4, 775–783
Publication Date (Web):February 26, 2019
https://doi.org/10.1021/acschembio.9b00109
Copyright © 2019 American Chemical Society

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    Abstract

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    Cell-based therapy is a promising approach to restoring lost functions to compromised organs. However, the issue of inefficient cell engraftment remains to be resolved. Herein, we take a chemical approach to facilitate cell engraftment by using self-assembling molecules which modify two cellular traits: cell survival and invasiveness. In this system, the self-assembling molecule induces syndecan-4 clusters on the cellular surface, leading to enhanced cell viability. Further integration with Halo-tag technology provided this self-assembly structure with matrix metalloproteinase-2 to functionalize cells with cell-invasion activity. In vivo experiments showed that the pretreated cells were able to survive injection and then penetrate and engraft into the host tissue, demonstrating that the system enhances cell engraftment. Therefore, cell-surface modification via an alliance between self-assembling molecules and ligation technologies may prove to be a promising method for cell engraftment.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acschembio.9b00109.

    • Full materials and procedures for the synthesis of the compounds, experimental details of the characterization (ITC, CD, DLS, and EM), details for cellular experiments (cell viability assay, Western blotting, immunostaining, and flow cytometry), DNA construction (design and characterization of the recombinant protein), and animal experiments (PDF)

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