Visualizing and Profiling Lipids in the OVLT of Fat-1 and Wild Type Mouse Brains during LPS-Induced Systemic Inflammation Using AP-SMALDI MSIClick to copy article linkArticle link copied!
- Janne BredehöftJanne BredehöftInstitute of Veterinary Physiology and Biochemistry, Justus Liebig University Giessen, Frankfurter Strasse 100, D-35392 Giessen, GermanyMore by Janne Bredehöft
- Dhaka Ram BhandariDhaka Ram BhandariInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, D-35392 Giessen, GermanyMore by Dhaka Ram Bhandari
- Fabian Johannes PfliegerFabian Johannes PfliegerInstitute of Veterinary Physiology and Biochemistry, Justus Liebig University Giessen, Frankfurter Strasse 100, D-35392 Giessen, GermanyMore by Fabian Johannes Pflieger
- Sabine SchulzSabine SchulzInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, D-35392 Giessen, GermanyMore by Sabine Schulz
- Jing X. KangJing X. KangLaboratory for Lipid Medicine and Technology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129, United StatesMore by Jing X. Kang
- Sophie LayéSophie LayéUMR 1286, NutriNeuro: Laboratoire Nutrition et Neurobiologie Intégrée, Institut National de la Recherche Agronomique, Université de Bordeaux, Bordeaux 33076, FranceMore by Sophie Layé
- Joachim RothJoachim RothInstitute of Veterinary Physiology and Biochemistry, Justus Liebig University Giessen, Frankfurter Strasse 100, D-35392 Giessen, GermanyCenter for Mind, Brain and Behavior (CMBB), University of Marburg and Justus Liebig University Giessen, Marburg 35032, GermanyMore by Joachim Roth
- Rüdiger GerstbergerRüdiger GerstbergerInstitute of Veterinary Physiology and Biochemistry, Justus Liebig University Giessen, Frankfurter Strasse 100, D-35392 Giessen, GermanyMore by Rüdiger Gerstberger
- Konstantin MayerKonstantin MayerUniversity of Giessen and Marburg Lung Center (UGMLC), Justus Liebig University Giessen, Klinikstrasse 33, Giessen D-35392, GermanyMore by Konstantin Mayer
- Bernhard SpenglerBernhard SpenglerInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, D-35392 Giessen, GermanyMore by Bernhard Spengler
- Christoph Rummel*Christoph Rummel*E-mail: [email protected]Institute of Veterinary Physiology and Biochemistry, Justus Liebig University Giessen, Frankfurter Strasse 100, D-35392 Giessen, GermanyCenter for Mind, Brain and Behavior (CMBB), University of Marburg and Justus Liebig University Giessen, Marburg 35032, GermanyMore by Christoph Rummel
Abstract

Lipids, including omega-3 polyunsaturated fatty acids (n-3-PUFAs), modulate brain-intrinsic inflammation during systemic inflammation. The vascular organ of the lamina terminalis (OVLT) is a brain structure important for immune-to-brain communication. We, therefore, aimed to profile the distribution of several lipids (e.g., phosphatidyl-choline/ethanolamine, PC/PE), including n-3-PUFA-carrying lipids (esterified in phospholipids), in the OVLT during systemic lipopolysaccharide(LPS)-induced inflammation. We injected wild type and endogenously n-3-PUFA producing fat-1 transgenic mice with LPS (i.p., 2.5 mg/kg) or PBS. Brain samples were analyzed using immunohistochemistry and high-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization orbital trapping mass spectrometry imaging (AP-SMALDI-MSI) for spatial resolution of lipids. Depending on genotype and treatment, several distinct distribution patterns were observed for lipids [e.g., lyso(L)PC (16:0)/(18:0)] proposed to be involved in inflammation. The distribution patterns ranged from being homogeneously disseminated [LPC (18:1)], absent/reduced signaling within the OVLT relative to adjacent preoptic tissue [PE (38:6)], either treatment- and genotype-dependent or independent low signal intensities [LPC (18:0)], treatment- and genotype-dependent [PC 38:6)] or independent accumulation in the OVLT [PC (38:7)], and accumulation in commissures, e.g., nerve fibers like the optic nerve [LPE (18:1)]. Overall, screening of lipid distribution patterns revealed distinct inflammation-induced changes in the OVLT, highlighting the prominent role of lipid metabolism in brain inflammation. Moreover, known and novel candidates for brain inflammation and immune-to-brain communication were detected specifically within this pivotal brain structure, a window between the periphery and the brain. The biological significance of these newly identified lipids abundant in the OVLT and the adjacent preoptic area remains to be further analyzed.
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