Citrus sinensis Essential Oil-Based Microemulsions: Green Synthesis, Characterization, and Antibacterial and Larvicide ActivitiesClick to copy article linkArticle link copied!
- Jônatas SouzaJônatas SouzaInstitute of Technology and Research (ITP), Avenida Murilo Dantas 300, 49010-390 Aracaju, BrazilIndustrial Biotechnology, Tiradentes University (UNIT) Avenida Murilo Dantas 300, 49032-490 Aracaju, BrazilMore by Jônatas Souza
- Glaciene S. SantosGlaciene S. SantosFederal University of Sergipe (UFS), Avenida Marechal Rondon, 49100-000 São Cristovão, BrazilMore by Glaciene S. Santos
- Thallysson C. BarbosaThallysson C. BarbosaInstitute of Technology and Research (ITP), Avenida Murilo Dantas 300, 49010-390 Aracaju, BrazilIndustrial Biotechnology, Tiradentes University (UNIT) Avenida Murilo Dantas 300, 49032-490 Aracaju, BrazilMore by Thallysson C. Barbosa
- André L. S. SantosAndré L. S. SantosDepartamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, 21941, Rio de Janeiro, RJ BrazilMore by André L. S. Santos
- Lucas G. MenezesLucas G. MenezesDepartamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, 21941, Rio de Janeiro, RJ BrazilMore by Lucas G. Menezes
- Luciana N. AndradeLuciana N. AndradeFederal University of Sergipe (UFS), Avenida Marechal Rondon, 49100-000 São Cristovão, BrazilMore by Luciana N. Andrade
- Cristiane BaniCristiane BaniFederal University of Sergipe (UFS), Avenida Marechal Rondon, 49100-000 São Cristovão, BrazilMore by Cristiane Bani
- Victor H. V. SarmentoVictor H. V. SarmentoFederal University of Sergipe (UFS), Avenida Marechal Rondon, 49100-000 São Cristovão, BrazilMore by Victor H. V. Sarmento
- Rogéria NunesRogéria NunesFederal University of Sergipe (UFS), Avenida Marechal Rondon, 49100-000 São Cristovão, BrazilMore by Rogéria Nunes
- Aleksandra ZielinskaAleksandra ZielinskaDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, PortugalInstitute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznań, PolandMore by Aleksandra Zielinska
- Marcelo C. MendonçaMarcelo C. MendonçaInstitute of Technology and Research (ITP), Avenida Murilo Dantas 300, 49010-390 Aracaju, BrazilIndustrial Biotechnology, Tiradentes University (UNIT) Avenida Murilo Dantas 300, 49032-490 Aracaju, BrazilEmpresa de Desenvolvimento Agropecuário de Sergipe (ENDAGRO), Avenida Carlos Rodrigues da Cruz, s/n - Bairro Capucho, 49081-015, Aracaju, Sergipe BrazilMore by Marcelo C. Mendonça
- Juliana C. CardosoJuliana C. CardosoInstitute of Technology and Research (ITP), Avenida Murilo Dantas 300, 49010-390 Aracaju, BrazilIndustrial Biotechnology, Tiradentes University (UNIT) Avenida Murilo Dantas 300, 49032-490 Aracaju, BrazilMore by Juliana C. Cardoso
- Antonello Santini*Antonello Santini*E-mail: [email protected]Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131, Napoli, ItalyMore by Antonello Santini
- Eliana B. Souto*Eliana B. Souto*Email: [email protected]Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, PortugalCEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalMore by Eliana B. Souto
- Patrícia Severino*Patrícia Severino*E-mail: [email protected]Institute of Technology and Research (ITP), Avenida Murilo Dantas 300, 49010-390 Aracaju, BrazilIndustrial Biotechnology, Tiradentes University (UNIT) Avenida Murilo Dantas 300, 49032-490 Aracaju, BrazilTiradentes Institute, 150 Mt, Vernon Street, Dorchester, Massachusetts 02125, Unites StatesDivision of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women’s Hospital, Cambridge, Massachusetts 02139, Unites StatesMore by Patrícia Severino
Abstract
The aim of this study was the development and characterization of a new microemulsion composed of sweet orange (Citrus sinensis L.) essential oils (EOCs) and evaluate its antibacterial and larvicide profiles. The optimal combination of EOCs, surfactant (Kolliphor EL), and water was achieved using a ternary phase diagram. EOCs were obtained from orange peel by steam-distillation and analyzed by gas chromatograph–mass spectrometer (GC-MS). A total of 25 compounds were identified, among which 90 wt % was d-limonene. In order to determine antimicrobial activity of the formulation, minimum inhibitory concentration (MIC) assay was done. d-Limonene, limonene 1,2-epoxide, and β-mircene showed a high antibacterial activity. Cell viability studies were performed in L929 murine fibroblast as a cellular line, using the MTT assay. A cell viability of 118% for EOCs and 117.7% for microemulsion after 72 h was recorded indicating no relevant cytotoxic effect. In vivo studies, based on a wax moth, Galleria mellonella (G. mellonella), larvae as a model system showed 100% of larvae mortality under the influence of the microemulsion with EOCs, while a second efficiency rating of 45% for larvae mortality was reached for pure EOCs. These tests confirm a strong larvicide activity for the essential oil, characterized by the highest concentration of limonene as the larvicide agent. The loading of EOCs into microemulsions offered an improved approach for the delivery of this oil which retained its bioactivity, as shown in vitro and in vivo.
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