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Cannabinoids from Cannabis sativa L.: A New Tool Based on HPLC–DAD–MS/MS for a Rational Use in Medicinal Chemistry

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    Cannabinoids from Cannabis sativa L.: A New Tool Based on HPLC–DAD–MS/MS for a Rational Use in Medicinal Chemistry
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    • Michele Protti
      Michele Protti
      Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
      More by Michele Protti
    • Virginia Brighenti
      Virginia Brighenti
      Department of Life Sciences (DSV), University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
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    • Maria Rita Battaglia
      Maria Rita Battaglia
      Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
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    • Lisa Anceschi
      Lisa Anceschi
      Department of Life Sciences (DSV), University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
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    • Federica Pellati*
      Federica Pellati
      Department of Life Sciences (DSV), University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
      *Tel: +39-059-2058565. E-mail: [email protected]
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      Orcidhttp://orcid.org/0000-0002-9822-6862
    • Laura Mercolini
      Laura Mercolini
      Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy
      More by Laura Mercolini
      Orcidhttp://orcid.org/0000-0002-0644-9461
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    ACS Medicinal Chemistry Letters

    Cite this: ACS Med. Chem. Lett. 2019, 10, 4, 539–544
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    https://doi.org/10.1021/acsmedchemlett.8b00571
    Published January 29, 2019
    Copyright © 2019 American Chemical Society
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    Abstract

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    Cannabis sativa L. represents one of the most widely used source of drugs and drugs of abuse worldwide. Its biologically active compounds are mainly cannabinoids, including Δ9-tetrahydrocannabinol (THC), which is responsible for the psychoactive effects, tetrahydrocannabinolic acid (THCA), cannabinol (CBN), cannabidiol (CBD), and cannabidiolic acid (CBDA). Together with recreational and drug-type (or medicinal) Cannabis, some new products have been recently released into the market as fiber-type Cannabis variants (also known as hemp or industrial hemp) with low THC content and high content of nonpsychoactive CBD. In this research work, the aim was to characterize Cannabis recreational and drug-type samples by quantifying their active principles, after the development and validation of a suitable analytical method. In addition to the Cannabis samples described above, fiber-type plant varieties were also analyzed to monitor their content of nonpsychoactive compounds for both pharmaceutical and nutraceutical purposes. To do this, a highly efficient HPLC–DAD–MS/MS method, with an electrospray ionization (ESI) source and a triple-quadrupole mass analyzer acquiring in the multiple reaction monitoring (MRM) mode also coupled to a diode array detector (DAD), was developed and applied. Satisfactory validation results were obtained in terms of precision (RSD < 6.0% for all the analytes) and accuracy (>92.1% for all the compounds). The proposed methodology represents a versatile and reliable tool to assess both psychoactive and nonpsychoactive cannabinoid levels in Cannabis samples for a more rational use in both medicinal chemistry and nutraceutics.

    Copyright © 2019 American Chemical Society

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    ACS Medicinal Chemistry Letters

    Cite this: ACS Med. Chem. Lett. 2019, 10, 4, 539–544
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsmedchemlett.8b00571
    Published January 29, 2019
    Copyright © 2019 American Chemical Society
    Request reuse permissions

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