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    X-rays Reveal the Internal Structure of Keratin Bundles in Whole Cells
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    † Institute for X-ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany
    ‡ Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany
    Institute of Structural Physics, Technische Universität Dresden, Zellescher Weg 16, 01069 Dresden, Germany
    § European Synchrotron Radiation Facility, 71, Avenue des Martyrs, 38043 Grenoble, France
    ∥ Department of Analytical Chemistry, Ghent University, Krijgslaan 281, 9000 Ghent, Belgium
    ⊥ Institute for Nanostructure and Solid State Physics, Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany
    *E-mail: [email protected]
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    ACS Nano

    Cite this: ACS Nano 2016, 10, 3, 3553–3561
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    https://doi.org/10.1021/acsnano.5b07871
    Published February 23, 2016
    Copyright © 2016 American Chemical Society
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    Abstract

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    In recent years, X-ray imaging of biological cells has emerged as a complementary alternative to fluorescence and electron microscopy. Different techniques were established and successfully applied to macromolecular assemblies and structures in cells. However, while the resolution is reaching the nanometer scale, the dose is increasing. It is essential to develop strategies to overcome or reduce radiation damage. Here we approach this intrinsic problem by combing two different X-ray techniques, namely ptychography and nanodiffraction, in one experiment and on the same sample. We acquire low dose ptychography overview images of whole cells at a resolution of 65 nm. We subsequently record high-resolution nanodiffraction data from regions of interest. By comparing images from the two modalities, we can exclude strong effects of radiation damage on the specimen. From the diffraction data we retrieve quantitative structural information from intracellular bundles of keratin intermediate filaments such as a filament radius of 5 nm, hexagonal geometric arrangement with an interfilament distance of 14 nm and bundle diameters on the order of 70 nm. Thus, we present an appealing combined approach to answer a broad range of questions in soft-matter physics, biophysics and biology.

    Copyright © 2016 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsnano.5b07871.

    • Additional images of ptychogram reconstruction and corresponding dark field images; fitting procedure of the 2D patterns and examples of fitting results. (PDF)

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    This article is cited by 32 publications.

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    10. Reinhard Windoffer, Nicole Schwarz, Sungjun Yoon, Teodora Piskova, Michael Scholkemper, Johannes Stegmaier, Andrea Bönsch, Jacopo Di Russo, Rudolf E Leube. Quantitative mapping of keratin networks in 3D. eLife 2022, 11 https://doi.org/10.7554/eLife.75894
    11. Ehud Haimov, Reinhard Windoffer, Rudolf E. Leube, Michael Urbakh, Michael M. Kozlov. Model for Bundling of Keratin Intermediate Filaments. Biophysical Journal 2020, 119 (1) , 65-74. https://doi.org/10.1016/j.bpj.2020.05.024
    12. Chiara Cassini, Andrew Wittmeier, Gerrit Brehm, Manuela Denz, Manfred Burghammer, Sarah Köster. Large field-of-view scanning small-angle X-ray scattering of mammalian cells. Journal of Synchrotron Radiation 2020, 27 (4) , 1059-1068. https://doi.org/10.1107/S1600577520006864
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    14. , , , , Sungjun Yoon, Rudolf E. Leube. Keratin intermediate filaments: intermediaries of epithelial cell migration. Essays in Biochemistry 2019, 63 (5) , 521-533. https://doi.org/10.1042/EBC20190017
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    ACS Nano

    Cite this: ACS Nano 2016, 10, 3, 3553–3561
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsnano.5b07871
    Published February 23, 2016
    Copyright © 2016 American Chemical Society
    Request reuse permissions

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