Deterministic Encapsulation of Human Cardiac Stem Cells in Variable Composition Nanoporous Gel Cocoons To Enhance Therapeutic Repair of Injured MyocardiumClick to copy article linkArticle link copied!
- Pushpinder KandaPushpinder KandaUniversity of Ottawa Heart Institute, Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, Canada K1Y4W7More by Pushpinder Kanda
- Emilio I. AlarconEmilio I. AlarconDivision of Cardiac Surgery, Department of Surgery, University of Ottawa Heart Institute, University of Ottawa, Ottawa, Canada K1Y4W7Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada K1H8M5More by Emilio I. Alarcon
- Tanya YeuchykTanya YeuchykUniversity of Ottawa Heart Institute, Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, Canada K1Y4W7More by Tanya Yeuchyk
- Sandrine ParentSandrine ParentUniversity of Ottawa Heart Institute, Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, Canada K1Y4W7More by Sandrine Parent
- Robert A. de KempRobert A. de KempUniversity of Ottawa Heart Institute, Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, Canada K1Y4W7More by Robert A. de Kemp
- Fabio VariolaFabio VariolaDepartment of Mechanical Engineering, University of Ottawa, Ottawa, Canada K1N6N5Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada K1H8M5More by Fabio Variola
- David CourtmanDavid CourtmanRegenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada K1H8L6More by David Courtman
- Duncan J. StewartDuncan J. StewartUniversity of Ottawa Heart Institute, Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, Canada K1Y4W7Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada K1H8M5Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada K1H8L6More by Duncan J. Stewart
- Darryl R. Davis*Darryl R. Davis*E-mail: [email protected]University of Ottawa Heart Institute, Division of Cardiology, Department of Medicine, University of Ottawa, Ottawa, Canada K1Y4W7Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada K1H8M5More by Darryl R. Davis
Abstract

Although cocooning explant-derived cardiac stem cells (EDCs) in protective nanoporous gels (NPGs) prior to intramyocardial injection boosts long-term cell retention, the number of EDCs that finally engraft is trivial and unlikely to account for salutary effects on myocardial function and scar size. As such, we investigated the effect of varying the NPG content within capsules to alter the physical properties of cocoons without influencing cocoon dimensions. Increasing NPG concentration enhanced cell migration and viability while improving cell-mediated repair of injured myocardium. Given that the latter occurred with NPG content having no detectable effect on the long-term engraftment of transplanted cells, we found that changing the physical properties of cocoons prompted explant-derived cardiac stem cells to produce greater amounts of cytokines, nanovesicles, and microRNAs that boosted the generation of new blood vessels and new cardiomyocytes. Thus, by altering the physical properties of cocoons by varying NPG content, the paracrine signature of encapsulated cells can be enhanced to promote greater endogenous repair of injured myocardium.
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