Cross-Platform Cancer Cell Identification Using Telomerase-Specific Spherical Nucleic Acids
- Zhengjie LiuZhengjie LiuCAS Center for Excellence in Nanoscience, Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaDepartment of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Zhengjie Liu,
- Jun ZhaoJun ZhaoCAS Center for Excellence in Nanoscience, Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaMore by Jun Zhao,
- Ruilong ZhangRuilong ZhangSchool of Chemistry and Chemical Engineering, Anhui University, Hefei, Anhui 230601, ChinaMore by Ruilong Zhang,
- Guangmei HanGuangmei HanCAS Center for Excellence in Nanoscience, Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaDepartment of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, ChinaMore by Guangmei Han,
- Cheng ZhangCheng ZhangCAS Center for Excellence in Nanoscience, Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaMore by Cheng Zhang,
- Bianhua LiuBianhua LiuCAS Center for Excellence in Nanoscience, Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaMore by Bianhua Liu,
- Zhongping Zhang*Zhongping Zhang*E-mail: [email protected]CAS Center for Excellence in Nanoscience, Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaSchool of Chemistry and Chemical Engineering, Anhui University, Hefei, Anhui 230601, ChinaMore by Zhongping Zhang,
- Ming-Yong HanMing-Yong HanCAS Center for Excellence in Nanoscience, Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaInstitute of Materials Research and Engineering, A-STAR, 3 Research Link, Singapore 117602More by Ming-Yong Han, and
- Xiaohu Gao*Xiaohu Gao*E-mail: [email protected]Department of Bioengineering, University of Washington, Seattle, Washington 98195, United StatesMore by Xiaohu Gao
Abstract
Distinguishing tumor cells from normal cells holds the key to precision diagnosis and effective intervention of cancers. The fundamental difficulties, however, are the heterogeneity of tumor cells and the lack of truly specific and ideally universal cancer biomarkers. Here, we report a concept of tumor cell detection, bypassing the specific genotypic and phenotypic features of different tumor cell types and directly going toward the hallmark of cancer, uncontrollable growth. Combining spherical nucleic acids (SNAs) with exquisitely engineered molecular beacons (SNA beacons, dubbed SNAB technology) is capable of identifying tumor cells from normal cells based on the molecular phenotype of telomerase activity, largely bypassing the heterogeneity problem of cancers. Owing to the cell-entry capability of SNAs, the SNAB probe readily achieves tumor cell detection across multiple platforms, ranging from solution-based assay, to single cell imaging and in vivo solid tumor imaging (unlike PCR that is restricted to cell lysates). We envision the SNAB technology will impact cancer diagnosis, therapeutic response assessment, and image-guided surgery.
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