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Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics

  • Baoliang Ge
    Baoliang Ge
    Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    More by Baoliang Ge
  • Qing Zhang
    Qing Zhang
    Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    More by Qing Zhang
  • Rui Zhang
    Rui Zhang
    Department of Physics, The Hong Kong University of Science and Technology, Hong Kong, 999077, China
    More by Rui Zhang
  • Jing-Tang Lin
    Jing-Tang Lin
    Department of Physics, National Taiwan University, Taipei 106, Taiwan, Republic of China
  • Po-Hang Tseng
    Po-Hang Tseng
    Department of Physics, National Taiwan University, Taipei 106, Taiwan, Republic of China
  • Che-Wei Chang
    Che-Wei Chang
    Department of Physics, National Taiwan University, Taipei 106, Taiwan, Republic of China
  • Chen-Yuan Dong
    Chen-Yuan Dong
    Department of Physics, National Taiwan University, Taipei 106, Taiwan, Republic of China
  • Renjie Zhou
    Renjie Zhou
    Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, 999077, China
    More by Renjie Zhou
  • Zahid Yaqoob
    Zahid Yaqoob
    Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    More by Zahid Yaqoob
  • Irmgard Bischofberger
    Irmgard Bischofberger
    Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
  • , and 
  • Peter T. C. So*
    Peter T. C. So
    Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States
    *E-mail: [email protected]
Cite this: ACS Photonics 2021, 8, 12, 3440–3447
Publication Date (Web):November 17, 2021
https://doi.org/10.1021/acsphotonics.1c00788
Copyright © 2021 American Chemical Society

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    Abstract

    Abstract Image

    Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350 × 350 μm2 and a diffraction-limit spatial resolution of ∼2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions.

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acsphotonics.1c00788.

    • A description of the validation of PSIM’s measurement accuracy using a rotating wave plate; a comparison of imaging results of PSIM and conventional Polarization Light Microscopy (PLM); an analysis of the temporal sensitivity and spatial sensitivity; the calibration procedure for the retrieved parameters; a description of the image registration method; a visualization of the retrieved parameters via tracking the temporal evolution of patterns emerging in flowing DSCG solutions (PDF)

    • Visualization of the emerging patterns in flowing DSCG Video 1: The quiver plot of a selected low retardance region’s change(AVI)

    • Visualization of the emerging patterns in flowing DSCG Video 2: The tracking of a selected low-retardance region (AVI)

    • Visualization of the emerging patterns in flowing DSCG Video 3: The quiver plot of the DSCG flow within the entire field-of-view(AVI)

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    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 4 publications.

    1. Mohit Rathor, Shivam Kumar Chaubey, Rupen Tamang, Biplob Koch, Rakesh Kumar Singh. Single-Shot Quantitative Polarization Imaging of Live Cancer Cells. ACS Photonics 2023, 10 (10) , 3755-3762. https://doi.org/10.1021/acsphotonics.3c00963
    2. Qing Zhang, Shuang Zhou, Rui Zhang, Irmgard Bischofberger. Dendritic patterns from shear-enhanced anisotropy in nematic liquid crystals. Science Advances 2023, 9 (2) https://doi.org/10.1126/sciadv.abq6820
    3. Juheon Lee, Seungwoo Shin, Herve Hugonnet, YongKeun Park. Spatially multiplexed dielectric tensor tomography. Optics Letters 2022, 47 (23) , 6205. https://doi.org/10.1364/OL.474969
    4. Shiqi Xu, Xiang Dai, Xi Yang, Kevin C. Zhou, Kanghyun Kim, Vinayak Pathak, Carolyn Glass, Roarke Horstmeyer. Tensorial tomographic differential phase-contrast microscopy. 2022, 1-11. https://doi.org/10.1109/ICCP54855.2022.9887674