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Nanoporous Anodic Alumina-Based Sensor for miR-99a-5p Detection as an Effective Early Breast Cancer Diagnostic Tool

  • Iris Garrido-Cano
    Iris Garrido-Cano
    Biomedical Research Institute INCLIVA, 46010 Valencia, Spain
    CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28029 Madrid, Spain
  • Luis Pla
    Luis Pla
    CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28029 Madrid, Spain
    Unidad Mixta de Investigación en Nanomedicina y Sensores, Universitat Politècnica de València, Instituto deInvestigación Sanitaria La Fe, 46026 Valencia, Spain
    Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46010 Valencia, Spain
    More by Luis Pla
  • Sara Santiago-Felipe*
    Sara Santiago-Felipe
    CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28029 Madrid, Spain
    Unidad Mixta de Investigación en Nanomedicina y Sensores, Universitat Politècnica de València, Instituto deInvestigación Sanitaria La Fe, 46026 Valencia, Spain
    Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46010 Valencia, Spain
    *Email: [email protected]
  • Soraya Simón
    Soraya Simón
    Biomedical Research Institute INCLIVA, 46010 Valencia, Spain
    Clinical Oncology Department, Hospital Clínico Universitario de Valencia, 46010 Valencia, Spain
  • Belen Ortega
    Belen Ortega
    Biomedical Research Institute INCLIVA, 46010 Valencia, Spain
    Clinical Oncology Department, Hospital Clínico Universitario de Valencia, 46010 Valencia, Spain
    More by Belen Ortega
  • Begoña Bermejo
    Begoña Bermejo
    Biomedical Research Institute INCLIVA, 46010 Valencia, Spain
    Clinical Oncology Department, Hospital Clínico Universitario de Valencia, 46010 Valencia, Spain
  • Ana Lluch
    Ana Lluch
    Biomedical Research Institute INCLIVA, 46010 Valencia, Spain
    Clinical Oncology Department, Hospital Clínico Universitario de Valencia, 46010 Valencia, Spain
    Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 46010 Valencia, Spain
    Universitat de València, 46010 Valencia, Spain
    More by Ana Lluch
  • Juan Miguel Cejalvo
    Juan Miguel Cejalvo
    Biomedical Research Institute INCLIVA, 46010 Valencia, Spain
    Clinical Oncology Department, Hospital Clínico Universitario de Valencia, 46010 Valencia, Spain
    Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 46010 Valencia, Spain
  • Pilar Eroles
    Pilar Eroles
    Biomedical Research Institute INCLIVA, 46010 Valencia, Spain
    Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 46010 Valencia, Spain
    COST Action CA15204, 1210 Brussels, Belgium
    More by Pilar Eroles
  • , and 
  • Ramón Martínez-Máñez*
    Ramón Martínez-Máñez
    CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28029 Madrid, Spain
    Unidad Mixta de Investigación en Nanomedicina y Sensores, Universitat Politècnica de València, Instituto deInvestigación Sanitaria La Fe, 46026 Valencia, Spain
    Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46010 Valencia, Spain
    Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València, Centro de Investigación Príncipe, 46012 Valencia, Spain
    *Email: [email protected]
Cite this: ACS Sens. 2021, 6, 3, 1022–1029
Publication Date (Web):February 18, 2021
https://doi.org/10.1021/acssensors.0c02222
Copyright © 2021 American Chemical Society

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    Abstract

    Abstract Image

    Circulating microRNAs have emerged as potential diagnostic biomarkers. The deregulation of the microRNA miR-99a-5p has been previously described as an effective biomarker of early breast cancer. Herein, we present a new nanoporous anodic alumina (NAA)-based biosensor that can detect plasma miR-99a-5p with high sensitivity and selectivity. NAA pores are loaded with rhodamine B and capped with a specific oligonucleotide that is able to block cargo release until the target is present. In the presence of miR-99a-5p, the capping oligonucleotide recognizes the miR-99a-5p sequence and displaces it allowing the release of the encapsulated dye. This method is able to successfully distinguish healthy controls from breast cancer patients, even at early stages with high efficiency, showing the presented system as a promising tool for breast cancer detection.

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    Cited By

    This article is cited by 10 publications.

    1. Dario Siniscalco, Umberto Galderisi, Gianfranco Peluso, Mauro Finicelli. Circulating microRNAs in Cancer: A 5-Year Update with a Focus on Breast and Lung Cancers. International Journal of Molecular Sciences 2024, 25 (6) , 3140. https://doi.org/10.3390/ijms25063140
    2. Masood Abu-Halima, Andreas Keller, Lea Simone Becker, Ulrike Fischer, Annika Engel, Nicole Ludwig, Fabian Kern, Trine B. Rounge, Hilde Langseth, Eckart Meese, Verena Keller. Dynamic and static circulating cancer microRNA biomarkers – a validation study. RNA Biology 2023, 20 (1) , 1-9. https://doi.org/10.1080/15476286.2022.2154470
    3. W. Yu, X. Nan, M. Schroyen, Y. Wang, B. Xiong. Inulin-induced differences on serum extracellular vesicles derived miRNAs in dairy cows suffering from subclinical mastitis. animal 2023, 17 (9) , 100954. https://doi.org/10.1016/j.animal.2023.100954
    4. Ali Akbar Ashkarran, Zijin Lin, Jatin Rana, Harvey Bumpers, Lorenzo Sempere, Morteza Mahmoudi. Impact of Nanomedicine in Women's Metastatic Breast Cancer. Small 2023, 20 https://doi.org/10.1002/smll.202301385
    5. Guoliang Shang, Dongxue Bi, Vladimir Semenovich Gorelik, Guangtao Fei, Lide Zhang. Anodic alumina photonic crystals: Structure engineering, optical properties and prospective applications. Materials Today Communications 2023, 34 , 105052. https://doi.org/10.1016/j.mtcomm.2022.105052
    6. Elisabet Xifre‐Perez, Josep Ferre‐Borrull, Lluis F. Marsal. Oligonucleotic Probes and Immunosensors Based on Nanoporous Anodic Alumina for Screening of Diseases. Advanced Materials Technologies 2022, 7 (9) https://doi.org/10.1002/admt.202101591
    7. Xiangrong Liu, Dimitri Papukashvili, Zhixiang Wang, Yan Liu, Xiaoxia Chen, Jianrong Li, Zhiyuan Li, Linjie Hu, Zheng Li, Nino Rcheulishvili, Xiaoqing Lu, Jinfeng Ma. Potential utility of miRNAs for liquid biopsy in breast cancer. Frontiers in Oncology 2022, 12 https://doi.org/10.3389/fonc.2022.940314
    8. S. Sornambikai, H. Amir, G. Bhuvaneshwari, N. Ponpandian, C. Viswanathan. Review—Systematic Review on Electrochemical Biosensing of Breast Cancer miRNAs to Develop Alternative DCIS Diagnostic Tool. ECS Sensors Plus 2022, 1 (2) , 021602. https://doi.org/10.1149/2754-2726/ac75c5
    9. Fenglin Li, Xixin Wang, Miao Zhu, Dongxin Liu, Dong Liu, Jianling Zhao. Fluorescence properties of fluorescein and rhodamine supported on alumina nanowire films. Ceramics International 2022, 48 (8) , 11181-11191. https://doi.org/10.1016/j.ceramint.2021.12.338
    10. Meng Yang, Binbin Yang, Danqi Deng. Targeting of EIF4EBP1 by miR‐99a‐3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression. Journal of Cellular and Molecular Medicine 2021, 25 (21) , 10291-10305. https://doi.org/10.1111/jcmm.16991

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